RchiOBHm_Chr5g0002131

ribosomal protein L4

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0002609
Physical Location & Seq
Forward (+)
1217875 .. 1221763
3889 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ28351

Sequence Viewer

Length: 924 bp
ATGGCTTTATCGGTTTCCAGAAGGCTTCTACGTTCATTTGGTTCTGTTTTGGCATCAGGCAGCTATGACTCTCCGAGTATATTATATCCATCGTTTCGCGCTTCTCATGGGGTTATCTTTTCCTCTGAGGAATCTTCGGCTGTTCCCAAGGGTTGGTCATCTTCTTATGCTTATCGAAGGCTTTCAACTCTGACTCCTGAATCAAGTGGAGGTGCATTTCCCTCTGATCTATTATCCAAAAAGCCTGTGGTAGCGTCAGAGCATAAAATAGGAAATTATCAAGACCTTGTAATTCCAGTGACAAATTTTCATAATGAAGAAAAAGGCTTGATGGTCTTGCCTGGTGATGTTTTTGATGTTCCTATTAGAAAGGATATTGTTCACCGGGTTGTACTATGGCAGCTAGCAAAAAGGCAACAGGGAACACATTCAACAAAAACTATCAGTGAGGTCAGCGGGACTGGGAGAAAGCCATGGAATCAGAAGGGTACTGGTCGAGCGAGGCATGGGACTTTGCGTGGTCCTCAGTTCCGAGGAGGTGCGACAATGCATGGTCCTAAGCCACGAAGTCATGCTATCAAGCTGAATAAGAAGGTTCGGCGTCTTGGGTTAAAAGTTGCTCTGACAGCTCGAGCAGCAGAGGGAAAGCTTCTTGTTTTCGAGGATTTGGAAATCCCCACACATAAAACCAAGAATATTGTGAACTATGTCAAGCAAATGGAGAACACCAAGAAAGTTCTAGTGGTGGATGGCGGCCCCATAAGTGATAAACTCAAGCTGGCAACTCAAAACATACATTATGTGAACGTGCTGCCCTCAATTGGTTTGAATGTCTACAGCATCCTACAGCATGACACACTGGTGATGTCTCGCGATGCTATAGACAAAATTGTTGAACGGATGCACACTCCAATTAACCGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L4

Protein Analysis

307

Amino Acids

33.93

Weight (kDa)

10.2

Isoelectric Point (pI)

50.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L4 PF00573 113 - 300 1.5e-70 Ribosomal protein L4/L1 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 153
AccI GTMKAC 1 cut(s) 834
AccII CGCG 2 cut(s) 99, 873
AciI CCGC 2 cut(s) 456, 753
AcsI RAATTY 1 cut(s) 304
AcyI GRCGYC 1 cut(s) 601
AfaI GTAC 2 cut(s) 393, 490
AfiI CCNNNNNNNGG 2 cut(s) 153, 821
AgsI TTSAA 4 cut(s) 186, 432, 829, 896
AjnI CCWGG 1 cut(s) 340
AleI CACNNNNGTG 1 cut(s) 860
AluBI AGCT 6 cut(s) 63, 403, 583, 629, 649, 778
AluI AGCT 6 cut(s) 63, 403, 583, 629, 649, 778
Alw26I GTCTC 1 cut(s) 873
Ama87I CYCGRG 1 cut(s) 630
AoxI GGCC 1 cut(s) 754
ApeKI GCWGC 4 cut(s) 60, 400, 635, 811
ApoI RAATTY 1 cut(s) 304
Asp700I GAANNNNTTC 2 cut(s) 181, 427
AspLEI GCGC 1 cut(s) 101
AspS9I GGNCC 3 cut(s) 521, 554, 755
AsuC2I CCSGG 1 cut(s) 386
AsuHPI GGTGA 3 cut(s) 356, 374, 874
AsuNHI GCTAGC 1 cut(s) 403
AvaI CYCGRG 1 cut(s) 630
AvaII GGWCC 2 cut(s) 521, 554
BbvI GCAGC 4 cut(s) 72, 412, 647, 798
BccI CCATC 3 cut(s) 97, 325, 743
BciT130I CCWGG 1 cut(s) 342
BcnI CCSGG 1 cut(s) 386
BcoDI GTCTC 1 cut(s) 873
BfaI CTAG 2 cut(s) 404, 740
BfmI CTRYAG 3 cut(s) 835, 845, 879
BisI GCNGC 5 cut(s) 61, 401, 636, 754, 812
BlsI GCNGC 5 cut(s) 62, 402, 637, 755, 813
Bme1390I CCNGG 2 cut(s) 342, 386
Bme18I GGWCC 2 cut(s) 521, 554
BmeT110I CYCGRG 1 cut(s) 630
BmgT120I GGNCC 3 cut(s) 521, 554, 755
BmiI GGNNCC 1 cut(s) 757
BmrFI CCNGG 2 cut(s) 342, 386
BmrI ACTGGG 1 cut(s) 471
BmsI GCATC 4 cut(s) 62, 849, 865, 891
BmtI GCTAGC 1 cut(s) 407
BmuI ACTGGG 1 cut(s) 471
Bpu10I CCTNAGC 1 cut(s) 558
BpuEI CTTGAG 1 cut(s) 758
BpuMI CCSGG 1 cut(s) 386
BsaHI GRCGYC 1 cut(s) 601
BsaJI CCNNGG 3 cut(s) 147, 473, 532
BsaXI ACNNNNNCTCC 2 cut(s) 178, 208
Bsc4I CCNNNNNNNGG 2 cut(s) 153, 821
Bse1I ACTGG 4 cut(s) 296, 466, 496, 864
BseBI CCWGG 1 cut(s) 342
BseDI CCNNGG 3 cut(s) 147, 473, 532
BseGI GGATG 3 cut(s) 754, 840, 906
BseLI CCNNNNNNNGG 2 cut(s) 153, 821
BseMII CTCAG 2 cut(s) 117, 539
BseNI ACTGG 4 cut(s) 296, 466, 496, 864
BseRI GAGGAG 1 cut(s) 549
BseXI GCAGC 4 cut(s) 72, 412, 647, 798
Bsh1236I CGCG 2 cut(s) 99, 873
BshFI GGCC 1 cut(s) 756
BsiHKCI CYCGRG 1 cut(s) 630
BsiSI CCGG 1 cut(s) 385
BslFI GGGAC 2 cut(s) 472, 523
BslI CCNNNNNNNGG 2 cut(s) 153, 821
BsmAI GTCTC 1 cut(s) 873
BsmFI GGGAC 2 cut(s) 472, 523
BsnI GGCC 1 cut(s) 756
BsoBI CYCGRG 1 cut(s) 630
Bsp143I GATC 1 cut(s) 226
Bsp19I CCATGG 1 cut(s) 473
Bsp68I TCGCGA 1 cut(s) 873
BspACI CCGC 2 cut(s) 456, 753
BspANI GGCC 1 cut(s) 756
BspCNI CTCAG 2 cut(s) 118, 538
BspFNI CGCG 2 cut(s) 99, 873
BspLI GGNNCC 1 cut(s) 757
BspOI GCTAGC 1 cut(s) 407
BsrI ACTGG 4 cut(s) 296, 466, 496, 864
BssECI CCNNGG 3 cut(s) 147, 473, 532
BssMI GATC 1 cut(s) 226
BssNI GRCGYC 1 cut(s) 601
BssT1I CCWWGG 2 cut(s) 147, 473
Bst2UI CCWGG 1 cut(s) 342
Bst4CI ACNGT 1 cut(s) 920
BstACI GRCGYC 1 cut(s) 601
BstC8I GCNNGC 2 cut(s) 405, 780
BstDEI CTNAG 3 cut(s) 126, 525, 558
BstDSI CCRYGG 1 cut(s) 473
BstF5I GGATG 3 cut(s) 754, 840, 906
BstFNI CGCG 2 cut(s) 99, 873
BstHHI GCGC 1 cut(s) 101
BstKTI GATC 1 cut(s) 229
BstMAI GTCTC 1 cut(s) 873
BstMBI GATC 1 cut(s) 226
BstMWI GCNNNNNNNGC 2 cut(s) 626, 635
BstNI CCWGG 1 cut(s) 342
BstSCI CCNGG 2 cut(s) 340, 384
BstSFI CTRYAG 3 cut(s) 835, 845, 879
BstUI CGCG 2 cut(s) 99, 873
BstV1I GCAGC 4 cut(s) 72, 412, 647, 798
BsuRI GGCC 1 cut(s) 756
BtgI CCRYGG 1 cut(s) 473
BtgZI GCGATG 1 cut(s) 888
BtsCI GGATG 3 cut(s) 754, 840, 906
BtsIMutI CAGTG 3 cut(s) 303, 451, 857
BtuMI TCGCGA 1 cut(s) 873
Cac8I GCNNGC 2 cut(s) 405, 780
CfoI GCGC 1 cut(s) 101
Cfr13I GGNCC 3 cut(s) 521, 554, 755
CseI GACGC 2 cut(s) 243, 590
Csp6I GTAC 2 cut(s) 392, 489
CviAII CATG 6 cut(s) 107, 474, 506, 551, 572, 851
CviQI GTAC 2 cut(s) 392, 489
DdeI CTNAG 3 cut(s) 126, 525, 558
DpnI GATC 1 cut(s) 228
DpnII GATC 1 cut(s) 226
Eco130I CCWWGG 2 cut(s) 147, 473
Eco47I GGWCC 2 cut(s) 521, 554
Eco88I CYCGRG 1 cut(s) 630
EcoRII CCWGG 1 cut(s) 340
EcoT14I CCWWGG 2 cut(s) 147, 473
EcoT22I ATGCAT 1 cut(s) 552
ErhI CCWWGG 2 cut(s) 147, 473
FaeI CATG 6 cut(s) 110, 477, 509, 554, 575, 854
FaqI GGGAC 2 cut(s) 472, 523
FatI CATG 6 cut(s) 106, 473, 505, 550, 571, 850
FauI CCCGC 1 cut(s) 449
FblI GTMKAC 1 cut(s) 834
Fnu4HI GCNGC 5 cut(s) 61, 401, 636, 754, 812
FokI GGATG 3 cut(s) 761, 827, 913
Fsp4HI GCNGC 5 cut(s) 61, 401, 636, 754, 812
FspBI CTAG 2 cut(s) 404, 740
GlaI GCGC 1 cut(s) 100
GluI GCNGC 5 cut(s) 61, 401, 636, 754, 812
HaeIII GGCC 1 cut(s) 756
HapII CCGG 1 cut(s) 385
HgaI GACGC 2 cut(s) 243, 590
HhaI GCGC 1 cut(s) 101
Hin1I GRCGYC 1 cut(s) 601
Hin1II CATG 6 cut(s) 110, 477, 509, 554, 575, 854
Hin6I GCGC 1 cut(s) 99
HinP1I GCGC 1 cut(s) 99
HindIII AAGCTT 1 cut(s) 647
HinfI GANTC 5 cut(s) 68, 131, 193, 200, 478
HpaII CCGG 1 cut(s) 385
HphI GGTGA 3 cut(s) 356, 374, 874
Hpy166II GTNNAC 4 cut(s) 382, 703, 805, 835
Hpy188I TCNGA 8 cut(s) 75, 127, 192, 226, 259, 483, 533, 624
Hpy188III TCNNGA 4 cut(s) 18, 197, 281, 872
Hpy8I GTNNAC 4 cut(s) 382, 703, 805, 835
HpyAV CCTTC 4 cut(s) 15, 171, 478, 586
HpyCH4III ACNGT 1 cut(s) 920
HpyCH4IV ACGT 2 cut(s) 31, 807
HpyCH4V TGCA 3 cut(s) 215, 550, 904
HpyF10VI GCNNNNNNNGC 2 cut(s) 626, 635
HpyF3I CTNAG 3 cut(s) 126, 525, 558
HpySE526I ACGT 2 cut(s) 31, 807
Hsp92I GRCGYC 1 cut(s) 601
Hsp92II CATG 6 cut(s) 110, 477, 509, 554, 575, 854
HspAI GCGC 1 cut(s) 99
Kzo9I GATC 1 cut(s) 226
Lsp1109I GCAGC 4 cut(s) 72, 412, 647, 798
LweI GCATC 4 cut(s) 62, 849, 865, 891
MaeI CTAG 2 cut(s) 404, 740
MaeII ACGT 2 cut(s) 31, 807
MaeIII GTNAC 1 cut(s) 298
MalI GATC 1 cut(s) 228
MboI GATC 1 cut(s) 226
MboII GAAGA 3 cut(s) 126, 153, 329
MfeI CAATTG 1 cut(s) 819
MluCI AATT 6 cut(s) 274, 291, 304, 819, 888, 912
MlyI GAGTC 2 cut(s) 62, 187
Mph1103I ATGCAT 1 cut(s) 552
MroXI GAANNNNTTC 2 cut(s) 181, 427
MseI TTAA 2 cut(s) 611, 915
MslI CAYNNNNRTG 1 cut(s) 860
MspA1I CMGCKG 1 cut(s) 456
MspI CCGG 1 cut(s) 385
MspR9I CCNGG 2 cut(s) 342, 386
MunI CAATTG 1 cut(s) 819
MvaI CCWGG 1 cut(s) 342
MvnI CGCG 2 cut(s) 99, 873
MwoI GCNNNNNNNGC 2 cut(s) 626, 635
NciI CCSGG 1 cut(s) 386
NcoI CCATGG 1 cut(s) 473
NdeII GATC 1 cut(s) 226
NheI GCTAGC 1 cut(s) 403
NlaIII CATG 6 cut(s) 110, 477, 509, 554, 575, 854
NlaIV GGNNCC 1 cut(s) 757
NmuCI GTSAC 1 cut(s) 298
NruI TCGCGA 1 cut(s) 873
NsiI ATGCAT 1 cut(s) 552
OliI CACNNNNGTG 1 cut(s) 860
PaeR7I CTCGAG 1 cut(s) 630
PdmI GAANNNNTTC 2 cut(s) 181, 427
PfeI GAWTC 3 cut(s) 131, 200, 478
PflMI CCANNNNNTGG 1 cut(s) 153
PkrI GCNGC 5 cut(s) 62, 402, 637, 755, 813
PleI GAGTC 2 cut(s) 62, 187
PpsI GAGTC 2 cut(s) 62, 187
Psp6I CCWGG 1 cut(s) 340
PspGI CCWGG 1 cut(s) 340
PspN4I GGNNCC 1 cut(s) 757
PspPI GGNCC 3 cut(s) 521, 554, 755
PspXI VCTCGAGB 1 cut(s) 630
RruI TCGCGA 1 cut(s) 873
RsaI GTAC 2 cut(s) 393, 490
RsaNI GTAC 2 cut(s) 392, 489
RseI CAYNNNNRTG 1 cut(s) 860
SaqAI TTAA 2 cut(s) 611, 915
SatI GCNGC 5 cut(s) 61, 401, 636, 754, 812
Sau3AI GATC 1 cut(s) 226
Sau96I GGNCC 3 cut(s) 521, 554, 755
SchI GAGTC 2 cut(s) 62, 187
ScrFI CCNGG 2 cut(s) 342, 386
SfaNI GCATC 4 cut(s) 62, 849, 865, 891
SfcI CTRYAG 3 cut(s) 835, 845, 879
Sfr274I CTCGAG 1 cut(s) 630
SinI GGWCC 2 cut(s) 521, 554
SlaI CTCGAG 1 cut(s) 630
SmiMI CAYNNNNRTG 1 cut(s) 860
SmlI CTYRAG 2 cut(s) 630, 773
SmoI CTYRAG 2 cut(s) 630, 773
Sse9I AATT 6 cut(s) 274, 291, 304, 819, 888, 912
SsiI CCGC 2 cut(s) 456, 753
SspI AATATT 1 cut(s) 697
SspMI CTAG 2 cut(s) 404, 740
StyD4I CCNGG 2 cut(s) 340, 384
StyI CCWWGG 2 cut(s) 147, 473
TaaI ACNGT 1 cut(s) 920
TaiI ACGT 2 cut(s) 34, 810
TaqI TCGA 4 cut(s) 175, 496, 631, 660
TasI AATT 6 cut(s) 274, 291, 304, 819, 888, 912
TatI WGTACW 1 cut(s) 391
TauI GCSGC 1 cut(s) 756
TfiI GAWTC 3 cut(s) 131, 200, 478
Tru1I TTAA 2 cut(s) 611, 915
Tru9I TTAA 2 cut(s) 611, 915
TscAI CASTG 3 cut(s) 303, 451, 864
TseFI GTSAC 1 cut(s) 298
TseI GCWGC 4 cut(s) 60, 400, 635, 811
Tsp45I GTSAC 1 cut(s) 298
TspDTI ATGAA 3 cut(s) 24, 299, 330
TspGWI ACGGA 1 cut(s) 913
TspRI CASTG 3 cut(s) 303, 451, 864
Van91I CCANNNNNTGG 1 cut(s) 153
VpaK11BI GGWCC 2 cut(s) 521, 554
XapI RAATTY 1 cut(s) 304
XcmI CCANNNNNNNNNTGG 1 cut(s) 244
XhoI CTCGAG 1 cut(s) 630
XmiI GTMKAC 1 cut(s) 834
XmnI GAANNNNTTC 2 cut(s) 181, 427
XspI CTAG 2 cut(s) 404, 740
Zsp2I ATGCAT 1 cut(s) 552
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.