FvH4_3g43740

ribosomal protein L4

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0002609
Physical Location & Seq
Reverse (-)
36431518 .. 36433718
2201 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g43740.t1

Sequence Viewer

Length: 474 bp
ATGAAAAGAAAGGATCCACAACTTTGGCTGGTGATGTTTATGATGCATCAATTACAATGGATTTGCTCGTCAGATCTTCTTCTTCTTTCGTTAGTTAAAATGTGGCAGCTAGCAAAAAGGCAACGGCCATCGAATTGGAAGGATAGTGGTCGAACCAGCCATGGAAGCTTGAGTGATCCCCAGCATAGAGGTGTCAACAGACGAAGTCATACTTCTAAGAATGTCGAACTTCTGGGACTAAAGATGGCTCTGTCATCTCGAGCGGCACAGGGAAAGCTTCTGGTTTTTGAGGATTTTGGAATCTACAGAAATAAACCCAATAACTATGTCAAAGAAAGAATGAAAAAGGAGAAGAAGAAAAAAATTCTGCTGGTGGATGGTGGTCCAGTAAGTAAAAAGCTCAAGTCGGCAACTGAACGTTTACGTCGTGTGACTGTGATGCCCTCAAGTGTAAGTCGCACATTCTGGTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L4

Protein Analysis

158

Amino Acids

18.33

Weight (kDa)

10.95

Isoelectric Point (pI)

54.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L4 PF00573 49 - 148 1.5e-07 Ribosomal protein L4/L1 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 263
AciI CCGC 1 cut(s) 263
AclI AACGTT 1 cut(s) 418
AclWI GGATC 3 cut(s) 8, 21, 170
AcoI YGGCCR 1 cut(s) 125
AcsI RAATTY 1 cut(s) 363
AluBI AGCT 4 cut(s) 109, 168, 277, 400
AluI AGCT 4 cut(s) 109, 168, 277, 400
AlwI GGATC 3 cut(s) 8, 21, 170
Ama87I CYCGRG 1 cut(s) 258
AoxI GGCC 1 cut(s) 125
ApeKI GCWGC 1 cut(s) 106
ApoI RAATTY 1 cut(s) 363
AspS9I GGNCC 1 cut(s) 383
AsuHPI GGTGA 1 cut(s) 43
AsuNHI GCTAGC 1 cut(s) 109
AvaI CYCGRG 1 cut(s) 258
AvaII GGWCC 1 cut(s) 383
BamHI GGATCC 1 cut(s) 13
BbvI GCAGC 1 cut(s) 118
BccI CCATC 3 cut(s) 136, 238, 371
BceAI ACGGC 1 cut(s) 140
BfaI CTAG 1 cut(s) 110
BfmI CTRYAG 1 cut(s) 304
BglII AGATCT 1 cut(s) 73
BisI GCNGC 2 cut(s) 107, 264
BlsI GCNGC 2 cut(s) 108, 265
Bme18I GGWCC 1 cut(s) 383
BmeT110I CYCGRG 1 cut(s) 258
BmgT120I GGNCC 1 cut(s) 383
BmiI GGNNCC 1 cut(s) 15
BmsI GCATC 3 cut(s) 33, 55, 429
BmtI GCTAGC 1 cut(s) 113
BpuEI CTTGAG 3 cut(s) 190, 386, 430
BsaJI CCNNGG 1 cut(s) 160
Bse1I ACTGG 1 cut(s) 386
BseDI CCNNGG 1 cut(s) 160
BseGI GGATG 1 cut(s) 382
BseNI ACTGG 1 cut(s) 386
BseXI GCAGC 1 cut(s) 118
BseYI CCCAGC 1 cut(s) 180
BshFI GGCC 1 cut(s) 127
BsiHKCI CYCGRG 1 cut(s) 258
BslFI GGGAC 1 cut(s) 249
BsmFI GGGAC 1 cut(s) 249
BsnI GGCC 1 cut(s) 127
BsoBI CYCGRG 1 cut(s) 258
Bsp143I GATC 3 cut(s) 13, 73, 175
Bsp19I CCATGG 1 cut(s) 160
BspACI CCGC 1 cut(s) 263
BspANI GGCC 1 cut(s) 127
BspLI GGNNCC 1 cut(s) 15
BspOI GCTAGC 1 cut(s) 113
BspPI GGATC 3 cut(s) 8, 21, 170
BsrBI CCGCTC 1 cut(s) 263
BsrI ACTGG 1 cut(s) 386
BssECI CCNNGG 1 cut(s) 160
BssMI GATC 3 cut(s) 13, 73, 175
BssT1I CCWWGG 1 cut(s) 160
Bst4CI ACNGT 1 cut(s) 436
BstC8I GCNNGC 1 cut(s) 111
BstDEI CTNAG 1 cut(s) 216
BstDSI CCRYGG 1 cut(s) 160
BstF5I GGATG 1 cut(s) 382
BstKTI GATC 3 cut(s) 16, 76, 178
BstMBI GATC 3 cut(s) 13, 73, 175
BstMWI GCNNNNNNNGC 1 cut(s) 165
BstSFI CTRYAG 1 cut(s) 304
BstV1I GCAGC 1 cut(s) 118
BstX2I RGATCY 2 cut(s) 13, 73
BstXI CCANNNNNNTGG 2 cut(s) 24, 135
BstYI RGATCY 2 cut(s) 13, 73
BsuRI GGCC 1 cut(s) 127
BtgI CCRYGG 1 cut(s) 160
BtsCI GGATG 1 cut(s) 382
Cac8I GCNNGC 1 cut(s) 111
Cfr13I GGNCC 1 cut(s) 383
CviAII CATG 1 cut(s) 161
CviJI RGCY 8 cut(s) 28, 109, 127, 159, 168, 248, 277, 400
CviKI_1 RGCY 8 cut(s) 28, 109, 127, 159, 168, 248, 277, 400
DdeI CTNAG 1 cut(s) 216
DpnI GATC 3 cut(s) 15, 75, 177
DpnII GATC 3 cut(s) 13, 73, 175
EaeI YGGCCR 1 cut(s) 125
Eco130I CCWWGG 1 cut(s) 160
Eco47I GGWCC 1 cut(s) 383
Eco88I CYCGRG 1 cut(s) 258
EcoT14I CCWWGG 1 cut(s) 160
EcoT22I ATGCAT 1 cut(s) 48
ErhI CCWWGG 1 cut(s) 160
FaeI CATG 1 cut(s) 164
FaiI YATR 5 cut(s) 41, 162, 186, 210, 327
FalI AAGNNNNNCTT 2 cut(s) 196, 228
FaqI GGGAC 1 cut(s) 249
FatI CATG 1 cut(s) 160
Fnu4HI GCNGC 2 cut(s) 107, 264
FokI GGATG 1 cut(s) 389
Fsp4HI GCNGC 2 cut(s) 107, 264
FspBI CTAG 1 cut(s) 110
GluI GCNGC 2 cut(s) 107, 264
GsaI CCCAGC 1 cut(s) 184
HaeIII GGCC 1 cut(s) 127
Hin1II CATG 1 cut(s) 164
HincII GTYRAC 1 cut(s) 196
HindII GTYRAC 1 cut(s) 196
HindIII AAGCTT 2 cut(s) 166, 275
HinfI GANTC 1 cut(s) 300
HphI GGTGA 1 cut(s) 43
Hpy166II GTNNAC 2 cut(s) 196, 422
Hpy188I TCNGA 1 cut(s) 73
Hpy188III TCNNGA 1 cut(s) 258
Hpy8I GTNNAC 2 cut(s) 196, 422
Hpy99I CGWCG 1 cut(s) 429
HpyAV CCTTC 1 cut(s) 133
HpyCH4III ACNGT 1 cut(s) 436
HpyCH4IV ACGT 2 cut(s) 418, 424
HpyCH4V TGCA 1 cut(s) 46
HpyF10VI GCNNNNNNNGC 1 cut(s) 165
HpyF3I CTNAG 1 cut(s) 216
HpySE526I ACGT 2 cut(s) 418, 424
Hsp92II CATG 1 cut(s) 164
Kzo9I GATC 3 cut(s) 13, 73, 175
LpnPI CCDG 9 cut(s) 14, 169, 194, 218, 254, 266, 356, 399, 451
Lsp1109I GCAGC 1 cut(s) 118
LweI GCATC 3 cut(s) 33, 55, 429
MaeI CTAG 1 cut(s) 110
MaeII ACGT 2 cut(s) 418, 424
MaeIII GTNAC 1 cut(s) 430
MalI GATC 3 cut(s) 15, 75, 177
MbiI CCGCTC 1 cut(s) 263
MboI GATC 3 cut(s) 13, 73, 175
MboII GAAGA 5 cut(s) 68, 71, 74, 364, 367
MflI RGATCY 2 cut(s) 13, 73
MluCI AATT 3 cut(s) 50, 133, 363
MnlI CCTC 3 cut(s) 182, 283, 454
Mph1103I ATGCAT 1 cut(s) 48
MseI TTAA 1 cut(s) 96
MslI CAYNNNNRTG 1 cut(s) 189
MwoI GCNNNNNNNGC 1 cut(s) 165
NcoI CCATGG 1 cut(s) 160
NdeII GATC 3 cut(s) 13, 73, 175
NheI GCTAGC 1 cut(s) 109
NlaIII CATG 1 cut(s) 164
NlaIV GGNNCC 1 cut(s) 15
NmuCI GTSAC 1 cut(s) 430
NsiI ATGCAT 1 cut(s) 48
PaeR7I CTCGAG 1 cut(s) 258
PcsI WCGNNNNNNNCGW 1 cut(s) 424
PfeI GAWTC 1 cut(s) 300
PflFI GACNNNGTC 1 cut(s) 204
PkrI GCNGC 2 cut(s) 108, 265
Psp1406I AACGTT 1 cut(s) 418
PspFI CCCAGC 1 cut(s) 180
PspN4I GGNNCC 1 cut(s) 15
PspPI GGNCC 1 cut(s) 383
PsuI RGATCY 2 cut(s) 13, 73
PsyI GACNNNGTC 1 cut(s) 204
RseI CAYNNNNRTG 1 cut(s) 189
SaqAI TTAA 1 cut(s) 96
SatI GCNGC 2 cut(s) 107, 264
Sau3AI GATC 3 cut(s) 13, 73, 175
Sau96I GGNCC 1 cut(s) 383
SetI ASST 7 cut(s) 111, 170, 193, 279, 402, 421, 427
SfaNI GCATC 3 cut(s) 33, 55, 429
SfcI CTRYAG 1 cut(s) 304
Sfr274I CTCGAG 1 cut(s) 258
SinI GGWCC 1 cut(s) 383
SlaI CTCGAG 1 cut(s) 258
SmiMI CAYNNNNRTG 1 cut(s) 189
SmlI CTYRAG 4 cut(s) 169, 258, 401, 445
SmoI CTYRAG 4 cut(s) 169, 258, 401, 445
Sse9I AATT 3 cut(s) 50, 133, 363
SsiI CCGC 1 cut(s) 263
SspMI CTAG 1 cut(s) 110
StyI CCWWGG 1 cut(s) 160
TaaI ACNGT 1 cut(s) 436
TaiI ACGT 2 cut(s) 421, 427
TaqI TCGA 4 cut(s) 131, 151, 225, 259
TasI AATT 3 cut(s) 50, 133, 363
TauI GCSGC 1 cut(s) 266
TfiI GAWTC 1 cut(s) 300
Tru1I TTAA 1 cut(s) 96
Tru9I TTAA 1 cut(s) 96
TseFI GTSAC 1 cut(s) 430
TseI GCWGC 1 cut(s) 106
Tsp45I GTSAC 1 cut(s) 430
TspDTI ATGAA 2 cut(s) 17, 356
Tth111I GACNNNGTC 1 cut(s) 204
VpaK11BI GGWCC 1 cut(s) 383
XapI RAATTY 1 cut(s) 363
XhoI CTCGAG 1 cut(s) 258
XspI CTAG 1 cut(s) 110
Zsp2I ATGCAT 1 cut(s) 48
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.