AT2G20840

Probably involved in membrane trafficking

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0003098
Physical Location & Seq
Reverse (-)
8971696 .. 8974555
2860 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G20840.2

Sequence Viewer

Length: 849 bp
ATGTCTCGCTACCAGTCTCATTCCTTTGATGACGGAGAAATTAATCCTTTTGCGAATCCTACAAGTGTCCCAGCTGCAACGTCAAAGCTTTCTCCTCTTCCCCCTGAGCCGTACGACCGTGGTGCCACAATGGATATTCCTCTTGACTCTGGAAAGGATCTGAAAGCTAAGGAGAAGGAACTCCGGGAAAAGGAGGCTGAATTGAAGAGGCGGGAGCAGGAAATCAAGCGGAAAGAAGATGCTATAGCACAGGCCGGAATTGTGATAGAAGAGAAAAACTGGCCACCGTTTTTTCCTCTCATCCATCATGACATTTCAAATGAGATACCCATCCATCTGCAGAGAATCCAATATGTTGCTTTCACATCAATGCTGGGTCTTGTGGTGTGCCTGTTATGGAACATTGTCGCAGTTACTACTGCGTGGATTAAGGGAGAAGGTCCAACAATATGGTTTCTAGCTATTATCTATTTCATATCAGGGGTTCCTGGAGCGTATGTTATGTGGTATCGCCCTCTTTATCGTGCCATGAGGACTGATAGTGCTCTGAAGTTTGGGTGGTTTTTCTTCACGTATCTGTTTCACATAGCGTTTTGTGTGTTTGCGGCGGTGGCTCCTCCAATTATCTTCAAGGGAAAATCGCTGACAGGAATATTGCCGGCGATCGACGTATTGAGTGGGAATATCTTGGTCGGGATATTTTACTTCATTGGGTTTGGGTTCTTCTGTCTGGAGTCACTCGTCAGCATCTGGGTTATTCAGCAAGTGTACATGTACTTCAGGGGGAGCGGGAAAGCAGCAGAGATGAAGCAGGAGGCCACAAGACGAGCAATGATGGCAGCTCTCTGA

Protein Analysis

282

Amino Acids

31.96

Weight (kDa)

8.32

Isoelectric Point (pI)

48.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SCAMP PF04144 48 - 219 5.1e-60 SCAMP family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 122
AccBSI CCGCTC 1 cut(s) 789
AciI CCGC 5 cut(s) 211, 229, 605, 608, 789
AclWI GGATC 1 cut(s) 165
AcoI YGGCCR 1 cut(s) 281
AcuI CTGAAG 2 cut(s) 569, 763
AfaI GTAC 3 cut(s) 113, 770, 776
AfiI CCNNNNNNNGG 1 cut(s) 190
AflIII ACRYGT 1 cut(s) 771
AgsI TTSAA 3 cut(s) 205, 318, 631
AjnI CCWGG 1 cut(s) 487
AluBI AGCT 5 cut(s) 74, 88, 167, 461, 842
AluI AGCT 5 cut(s) 74, 88, 167, 461, 842
Alw21I GWGCWC 1 cut(s) 547
Alw26I GTCTC 2 cut(s) 9, 21
AlwI GGATC 1 cut(s) 165
AlwNI CAGNNNCTG 1 cut(s) 750
AoxI GGCC 3 cut(s) 252, 281, 816
ApeKI GCWGC 3 cut(s) 74, 797, 839
AseI ATTAAT 1 cut(s) 42
AspS9I GGNCC 1 cut(s) 440
AsuC2I CCSGG 1 cut(s) 185
AvaII GGWCC 1 cut(s) 440
BalI TGGCCA 1 cut(s) 283
BanI GGYRCC 1 cut(s) 122
BarI GAAGNNNNNNTAC 2 cut(s) 761, 793
Bbv12I GWGCWC 1 cut(s) 547
BbvI GCAGC 2 cut(s) 61, 809
BccI CCATC 4 cut(s) 312, 338, 342, 829
BceAI ACGGC 1 cut(s) 94
BcgI CGANNNNNNTGC 2 cut(s) 104, 138
BciT130I CCWGG 1 cut(s) 489
BcnI CCSGG 1 cut(s) 185
BcoDI GTCTC 2 cut(s) 9, 21
BfaI CTAG 1 cut(s) 458
BfmI CTRYAG 2 cut(s) 243, 338
BisI GCNGC 4 cut(s) 75, 606, 798, 840
BlsI GCNGC 4 cut(s) 76, 607, 799, 841
Bme1390I CCNGG 2 cut(s) 185, 489
Bme18I GGWCC 1 cut(s) 440
BmgT120I GGNCC 1 cut(s) 440
BmiI GGNNCC 3 cut(s) 124, 486, 615
BmrFI CCNGG 2 cut(s) 185, 489
BmsI GCATC 2 cut(s) 229, 756
BpmI CTGGAG 2 cut(s) 510, 752
Bpu10I CCTNAGC 2 cut(s) 105, 168
BpuMI CCSGG 1 cut(s) 185
BsaAI YACGTR 1 cut(s) 573
BsaBI GATNNNNATC 1 cut(s) 329
BsaJI CCNNGG 1 cut(s) 118
BsaXI ACNNNNNCTCC 2 cut(s) 483, 513
Bsc4I CCNNNNNNNGG 1 cut(s) 190
Bse118I RCCGGY 1 cut(s) 658
Bse1I ACTGG 2 cut(s) 13, 284
Bse3DI GCAATG 1 cut(s) 837
Bse8I GATNNNNATC 1 cut(s) 329
BseBI CCWGG 1 cut(s) 489
BseDI CCNNGG 1 cut(s) 118
BseGI GGATG 2 cut(s) 300, 330
BseJI GATNNNNATC 1 cut(s) 329
BseLI CCNNNNNNNGG 1 cut(s) 190
BseMI GCAATG 1 cut(s) 837
BseMII CTCAG 1 cut(s) 96
BseNI ACTGG 2 cut(s) 13, 284
BseRI GAGGAG 2 cut(s) 84, 606
BseXI GCAGC 2 cut(s) 61, 809
BseYI CCCAGC 2 cut(s) 70, 373
Bsh1285I CGRYCG 2 cut(s) 118, 666
BshFI GGCC 3 cut(s) 254, 283, 818
BshNI GGYRCC 1 cut(s) 122
BsiEI CGRYCG 2 cut(s) 118, 666
BsiHKAI GWGCWC 1 cut(s) 547
BsiSI CCGG 3 cut(s) 184, 255, 659
BsiWI CGTACG 1 cut(s) 111
BslFI GGGAC 1 cut(s) 53
BslI CCNNNNNNNGG 1 cut(s) 190
BsmAI GTCTC 2 cut(s) 9, 21
BsmFI GGGAC 1 cut(s) 53
BsnI GGCC 3 cut(s) 254, 283, 818
Bsp1286I GDGCHC 1 cut(s) 547
Bsp1407I TGTACA 1 cut(s) 768
Bsp143I GATC 2 cut(s) 157, 663
BspACI CCGC 5 cut(s) 211, 229, 605, 608, 789
BspANI GGCC 3 cut(s) 254, 283, 818
BspCNI CTCAG 1 cut(s) 97
BspHI TCATGA 1 cut(s) 307
BspLI GGNNCC 3 cut(s) 124, 486, 615
BspMAI CTGCAG 1 cut(s) 342
BspPI GGATC 1 cut(s) 165
BspT107I GGYRCC 1 cut(s) 122
BsrBI CCGCTC 1 cut(s) 789
BsrDI GCAATG 1 cut(s) 837
BsrFI RCCGGY 1 cut(s) 658
BsrGI TGTACA 1 cut(s) 768
BsrI ACTGG 2 cut(s) 13, 284
BssAI RCCGGY 1 cut(s) 658
BssECI CCNNGG 1 cut(s) 118
BssMI GATC 2 cut(s) 157, 663
Bst2UI CCWGG 1 cut(s) 489
Bst4CI ACNGT 2 cut(s) 119, 288
Bst6I CTCTTC 3 cut(s) 102, 200, 264
BstAUI TGTACA 1 cut(s) 768
BstBAI YACGTR 1 cut(s) 573
BstC8I GCNNGC 1 cut(s) 660
BstDEI CTNAG 2 cut(s) 105, 168
BstDSI CCRYGG 1 cut(s) 118
BstF5I GGATG 2 cut(s) 300, 330
BstKTI GATC 2 cut(s) 160, 666
BstMAI GTCTC 2 cut(s) 9, 21
BstMBI GATC 2 cut(s) 157, 663
BstMCI CGRYCG 2 cut(s) 118, 666
BstMWI GCNNNNNNNGC 2 cut(s) 611, 836
BstNI CCWGG 1 cut(s) 489
BstNSI RCATGY 1 cut(s) 775
BstSCI CCNGG 2 cut(s) 183, 487
BstSFI CTRYAG 2 cut(s) 243, 338
BstV1I GCAGC 2 cut(s) 61, 809
BstX2I RGATCY 1 cut(s) 157
BstXI CCANNNNNNTGG 1 cut(s) 450
BstYI RGATCY 1 cut(s) 157
BsuRI GGCC 3 cut(s) 254, 283, 818
BtgI CCRYGG 1 cut(s) 118
BtsCI GGATG 2 cut(s) 300, 330
Cac8I GCNNGC 1 cut(s) 660
CaiI CAGNNNCTG 1 cut(s) 750
CciI TCATGA 1 cut(s) 307
Cfr10I RCCGGY 1 cut(s) 658
Cfr13I GGNCC 1 cut(s) 440
Csp6I GTAC 3 cut(s) 112, 769, 775
CviAII CATG 3 cut(s) 308, 529, 772
CviQI GTAC 3 cut(s) 112, 769, 775
DdeI CTNAG 2 cut(s) 105, 168
DpnI GATC 2 cut(s) 159, 665
DpnII GATC 2 cut(s) 157, 663
EaeI YGGCCR 1 cut(s) 281
Eam1104I CTCTTC 3 cut(s) 102, 200, 264
EarI CTCTTC 3 cut(s) 102, 200, 264
Eco47I GGWCC 1 cut(s) 440
Eco57I CTGAAG 2 cut(s) 569, 763
EcoRII CCWGG 1 cut(s) 487
FaeI CATG 3 cut(s) 311, 532, 775
FaqI GGGAC 1 cut(s) 53
FatI CATG 3 cut(s) 307, 528, 771
FauI CCCGC 2 cut(s) 204, 782
Fnu4HI GCNGC 4 cut(s) 75, 606, 798, 840
FokI GGATG 2 cut(s) 287, 317
Fsp4HI GCNGC 4 cut(s) 75, 606, 798, 840
FspBI CTAG 1 cut(s) 458
GluI GCNGC 4 cut(s) 75, 606, 798, 840
GsaI CCCAGC 2 cut(s) 74, 377
GsuI CTGGAG 2 cut(s) 510, 752
HaeIII GGCC 3 cut(s) 254, 283, 818
HapII CCGG 3 cut(s) 184, 255, 659
Hin1II CATG 3 cut(s) 311, 532, 775
HindIII AAGCTT 1 cut(s) 86
HinfI GANTC 4 cut(s) 55, 146, 345, 734
HpaII CCGG 3 cut(s) 184, 255, 659
Hpy166II GTNNAC 1 cut(s) 769
Hpy188I TCNGA 3 cut(s) 162, 549, 848
Hpy188III TCNNGA 5 cut(s) 143, 150, 308, 694, 731
Hpy8I GTNNAC 1 cut(s) 769
Hpy99I CGWCG 1 cut(s) 671
HpyAV CCTTC 2 cut(s) 169, 431
HpyCH4III ACNGT 2 cut(s) 119, 288
HpyCH4IV ACGT 3 cut(s) 80, 572, 669
HpyCH4V TGCA 2 cut(s) 77, 340
HpyF10VI GCNNNNNNNGC 2 cut(s) 611, 836
HpyF3I CTNAG 2 cut(s) 105, 168
HpySE526I ACGT 3 cut(s) 80, 572, 669
Hsp92II CATG 3 cut(s) 311, 532, 775
KroI GCCGGC 1 cut(s) 658
KroNI GCCGGC 1 cut(s) 660
Kzo9I GATC 2 cut(s) 157, 663
LmnI GCTCC 4 cut(s) 214, 491, 619, 786
Lsp1109I GCAGC 2 cut(s) 61, 809
LweI GCATC 2 cut(s) 229, 756
MaeI CTAG 1 cut(s) 458
MaeII ACGT 3 cut(s) 80, 572, 669
MaeIII GTNAC 2 cut(s) 412, 735
MalI GATC 2 cut(s) 159, 665
MbiI CCGCTC 1 cut(s) 789
MboI GATC 2 cut(s) 157, 663
MboII GAAGA 7 cut(s) 89, 217, 248, 281, 559, 619, 715
MflI RGATCY 1 cut(s) 157
MhlI GDGCHC 1 cut(s) 547
MlsI TGGCCA 1 cut(s) 283
MluCI AATT 4 cut(s) 39, 200, 258, 621
MluNI TGGCCA 1 cut(s) 283
MlyI GAGTC 2 cut(s) 140, 743
MmeI TCCRAC 1 cut(s) 467
MnlI CCTC 9 cut(s) 105, 150, 187, 201, 306, 525, 525, 627, 808
Mox20I TGGCCA 1 cut(s) 283
MroNI GCCGGC 1 cut(s) 658
MscI TGGCCA 1 cut(s) 283
MseI TTAA 2 cut(s) 42, 429
MslI CAYNNNNRTG 1 cut(s) 368
Msp20I TGGCCA 1 cut(s) 283
MspA1I CMGCKG 1 cut(s) 74
MspI CCGG 3 cut(s) 184, 255, 659
MspR9I CCNGG 2 cut(s) 185, 489
MvaI CCWGG 1 cut(s) 489
MwoI GCNNNNNNNGC 2 cut(s) 611, 836
NaeI GCCGGC 1 cut(s) 660
NciI CCSGG 1 cut(s) 185
NdeII GATC 2 cut(s) 157, 663
NgoMIV GCCGGC 1 cut(s) 658
NlaIII CATG 3 cut(s) 311, 532, 775
NlaIV GGNNCC 3 cut(s) 124, 486, 615
NmuCI GTSAC 1 cut(s) 735
NspI RCATGY 1 cut(s) 775
PagI TCATGA 1 cut(s) 307
PciI ACATGT 1 cut(s) 771
PdiI GCCGGC 1 cut(s) 660
PfeI GAWTC 2 cut(s) 55, 345
Pfl23II CGTACG 1 cut(s) 111
PfoI TCCNGGA 2 cut(s) 183, 487
PkrI GCNGC 4 cut(s) 76, 607, 799, 841
Ple19I CGATCG 1 cut(s) 666
PleI GAGTC 2 cut(s) 140, 742
PpsI GAGTC 2 cut(s) 140, 742
Ppu21I YACGTR 1 cut(s) 573
PscI ACATGT 1 cut(s) 771
PshBI ATTAAT 1 cut(s) 42
Psp6I CCWGG 1 cut(s) 487
PspFI CCCAGC 2 cut(s) 70, 373
PspGI CCWGG 1 cut(s) 487
PspLI CGTACG 1 cut(s) 111
PspN4I GGNNCC 3 cut(s) 124, 486, 615
PspPI GGNCC 1 cut(s) 440
PstI CTGCAG 1 cut(s) 342
PstNI CAGNNNCTG 1 cut(s) 750
PsuI RGATCY 1 cut(s) 157
PvuI CGATCG 1 cut(s) 666
PvuII CAGCTG 1 cut(s) 74
RsaI GTAC 3 cut(s) 113, 770, 776
RsaNI GTAC 3 cut(s) 112, 769, 775
RseI CAYNNNNRTG 1 cut(s) 368
SaqAI TTAA 2 cut(s) 42, 429
SatI GCNGC 4 cut(s) 75, 606, 798, 840
Sau3AI GATC 2 cut(s) 157, 663
Sau96I GGNCC 1 cut(s) 440
SchI GAGTC 2 cut(s) 140, 743
ScrFI CCNGG 2 cut(s) 185, 489
SduI GDGCHC 1 cut(s) 547
SetI ASST 9 cut(s) 76, 83, 90, 169, 442, 463, 575, 672, 844
SfaNI GCATC 2 cut(s) 229, 756
SfcI CTRYAG 2 cut(s) 243, 338
SinI GGWCC 1 cut(s) 440
SmiMI CAYNNNNRTG 1 cut(s) 368
Sse9I AATT 4 cut(s) 39, 200, 258, 621
SsiI CCGC 5 cut(s) 211, 229, 605, 608, 789
SspI AATATT 1 cut(s) 654
SspMI CTAG 1 cut(s) 458
StyD4I CCNGG 2 cut(s) 183, 487
TaaI ACNGT 2 cut(s) 119, 288
TaiI ACGT 3 cut(s) 83, 575, 672
TaqI TCGA 1 cut(s) 666
TasI AATT 4 cut(s) 39, 200, 258, 621
TatI WGTACW 2 cut(s) 768, 774
TauI GCSGC 1 cut(s) 608
TfiI GAWTC 2 cut(s) 55, 345
Tru1I TTAA 2 cut(s) 42, 429
Tru9I TTAA 2 cut(s) 42, 429
TseFI GTSAC 1 cut(s) 735
TseI GCWGC 3 cut(s) 74, 797, 839
Tsp45I GTSAC 1 cut(s) 735
TspDTI ATGAA 3 cut(s) 463, 697, 821
TspGWI ACGGA 1 cut(s) 48
VpaK11BI GGWCC 1 cut(s) 440
VspI ATTAAT 1 cut(s) 42
XceI RCATGY 1 cut(s) 775
XspI CTAG 1 cut(s) 458
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.