MD10G1300900.v1.1

Secretory carrier-associated membrane protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0003098
Physical Location & Seq
Forward (+)
38818114 .. 38822065
3952 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1300900.v1.1.491

Sequence Viewer

Length: 870 bp
ATGGCTGGTCGCTACGACAGTAACCCTTTTGCCGAAGAAGAAGAAGTCAATCCTTTCTCGAATCCTGGAATTGTTGCTCCTGCCAAAAACTCAAGACTTTCACCTCTTCCTCCTGAACGTGCTGGTTTCAACTACGGCCTTGGTGACCCCGTTGATATACCTATCGATGGAAATGCAGATTTGAAAAAGAGGGAGAGGGAACTCCAAGCTAAAGAAGCTGAATTGAGAAAGCGGGAGGAGATTGTAAGACGGAAAGAAGAAGCTGCCGCACGTGCTGGAATTGTTCTTGAGGAAAAAAATTGGCCACCTTTCTTCCCAATTATCCATCATGATATTGCAAATGAAATACCAATTCATTTGCAGAGGGTGCAGTATGTTGCATTCACAACATGGTTAGGACTTGTTCTTTGCCTTTTCTGGAATGTAATAGCTGTTACTACGGCATGGATTAAGGGGGAAGGGGTAAAAATTTGGTTCCTTGCTGTTATCTACTTCATATCAGGGGCTCCTGGATCCTATGTCTTGTGGTACCGTCCACTGTACCGTGTTTTCAGGTCCGAGAGTGCTTTAAAGTATGGATGGTTTTTCTTGTTTTATCTGATCCACATTGGCTTCTGCATCTTTGCTGCGGTTGCTCCTCCGATATTCTTCAAAGGAAAATCTCTCACTGGCATTCTGCCTGCAATTGATGTTCTTGGTGACCATGTTTTGGTTGGGATCTTCTACTTCATTGGATTTGGAATGTTCTGTCTTGAATCAGTGCTGAGCATCTGGGTCATTCAGCAAGTATACATGTATTTCCGAGGAAGCGGTAAAGCTGCTGAAATGAGGCGTGAAGGAGCGAGAGGAGTCGTGAGGGCAGCACTTTGA

Protein Analysis

290

Amino Acids

32.75

Weight (kDa)

8.66

Isoelectric Point (pI)

45.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SCAMP PF04144 98 - 269 2.7e-59 SCAMP family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 528
AccB1I GGYRCC 1 cut(s) 528
AccB7I CCANNNNNTGG 1 cut(s) 709
AccI GTMKAC 1 cut(s) 789
AciI CCGC 4 cut(s) 232, 267, 629, 810
AclWI GGATC 4 cut(s) 507, 520, 595, 725
AcoI YGGCCR 1 cut(s) 302
AcsI RAATTY 1 cut(s) 468
AcvI CACGTG 1 cut(s) 272
AfaI GTAC 2 cut(s) 530, 542
AfiI CCNNNNNNNGG 2 cut(s) 167, 709
AflIII ACRYGT 1 cut(s) 792
AgsI TTSAA 4 cut(s) 130, 184, 652, 755
AjnI CCWGG 2 cut(s) 64, 508
AluBI AGCT 5 cut(s) 209, 218, 263, 431, 818
AluI AGCT 5 cut(s) 209, 218, 263, 431, 818
AlwI GGATC 4 cut(s) 507, 520, 595, 725
AoxI GGCC 2 cut(s) 136, 302
ApeKI GCWGC 4 cut(s) 263, 626, 818, 860
ApoI RAATTY 1 cut(s) 468
ArsI GACNNNNNNTTYG 2 cut(s) 390, 422
Asp718I GGTACC 1 cut(s) 528
AspS9I GGNCC 1 cut(s) 555
AsuHPI GGTGA 3 cut(s) 93, 155, 710
AvaII GGWCC 1 cut(s) 555
BalI TGGCCA 1 cut(s) 304
BamHI GGATCC 1 cut(s) 512
BanI GGYRCC 1 cut(s) 528
BanII GRGCYC 1 cut(s) 508
BbrPI CACGTG 1 cut(s) 272
BbvI GCAGC 3 cut(s) 250, 613, 805
BccI CCATC 3 cut(s) 161, 333, 573
BceAI ACGGC 2 cut(s) 151, 456
BcgI CGANNNNNNTGC 2 cut(s) 155, 189
BciT130I CCWGG 2 cut(s) 66, 510
BisI GCNGC 5 cut(s) 264, 267, 627, 819, 861
BlpI GCTNAGC 1 cut(s) 764
BlsI GCNGC 5 cut(s) 265, 268, 628, 820, 862
Bme1390I CCNGG 2 cut(s) 66, 510
Bme18I GGWCC 1 cut(s) 555
BmgT120I GGNCC 1 cut(s) 555
BmiI GGNNCC 4 cut(s) 476, 507, 514, 530
BmrFI CCNGG 2 cut(s) 66, 510
BmsI GCATC 2 cut(s) 627, 777
Bpu1102I GCTNAGC 1 cut(s) 764
BpuEI CTTGAG 2 cut(s) 76, 308
Bsa29I ATCGAT 1 cut(s) 165
BsaAI YACGTR 1 cut(s) 272
BsaJI CCNNGG 2 cut(s) 139, 802
Bsc4I CCNNNNNNNGG 2 cut(s) 167, 709
Bse1I ACTGG 1 cut(s) 673
BseBI CCWGG 2 cut(s) 66, 510
BseCI ATCGAT 1 cut(s) 165
BseDI CCNNGG 2 cut(s) 139, 802
BseGI GGATG 1 cut(s) 584
BseLI CCNNNNNNNGG 2 cut(s) 167, 709
BseMII CTCAG 1 cut(s) 755
BseNI ACTGG 1 cut(s) 673
BseRI GAGGAG 3 cut(s) 251, 627, 861
BseXI GCAGC 3 cut(s) 250, 613, 805
BsgI GTGCAG 1 cut(s) 389
BshFI GGCC 2 cut(s) 138, 304
BshNI GGYRCC 1 cut(s) 528
BshVI ATCGAT 1 cut(s) 165
BslI CCNNNNNNNGG 2 cut(s) 167, 709
BsmI GAATGC 2 cut(s) 380, 672
BsnI GGCC 2 cut(s) 138, 304
Bsp1286I GDGCHC 1 cut(s) 508
Bsp143I GATC 3 cut(s) 512, 600, 717
Bsp1720I GCTNAGC 1 cut(s) 764
BspACI CCGC 4 cut(s) 232, 267, 629, 810
BspANI GGCC 2 cut(s) 138, 304
BspCNI CTCAG 1 cut(s) 756
BspDI ATCGAT 1 cut(s) 165
BspHI TCATGA 1 cut(s) 328
BspLI GGNNCC 4 cut(s) 476, 507, 514, 530
BspPI GGATC 4 cut(s) 507, 520, 595, 725
BspT107I GGYRCC 1 cut(s) 528
BsrI ACTGG 1 cut(s) 673
BssECI CCNNGG 2 cut(s) 139, 802
BssMI GATC 3 cut(s) 512, 600, 717
BssNAI GTATAC 1 cut(s) 790
BssT1I CCWWGG 1 cut(s) 139
Bst1107I GTATAC 1 cut(s) 790
Bst2UI CCWGG 2 cut(s) 66, 510
Bst4CI ACNGT 4 cut(s) 20, 533, 540, 545
Bst6I CTCTTC 1 cut(s) 111
BstAPI GCANNNNNTGC 1 cut(s) 367
BstBAI YACGTR 1 cut(s) 272
BstC8I GCNNGC 1 cut(s) 681
BstDEI CTNAG 1 cut(s) 764
BstEII GGTNACC 2 cut(s) 143, 698
BstF5I GGATG 1 cut(s) 584
BstKTI GATC 3 cut(s) 515, 603, 720
BstMBI GATC 3 cut(s) 512, 600, 717
BstMWI GCNNNNNNNGC 4 cut(s) 215, 272, 367, 632
BstNI CCWGG 2 cut(s) 66, 510
BstNSI RCATGY 1 cut(s) 796
BstPI GGTNACC 2 cut(s) 143, 698
BstSCI CCNGG 2 cut(s) 64, 508
BstV1I GCAGC 3 cut(s) 250, 613, 805
BstX2I RGATCY 2 cut(s) 512, 717
BstYI RGATCY 2 cut(s) 512, 717
BstZ17I GTATAC 1 cut(s) 790
Bsu15I ATCGAT 1 cut(s) 165
BsuRI GGCC 2 cut(s) 138, 304
BsuTUI ATCGAT 1 cut(s) 165
BtsCI GGATG 1 cut(s) 584
BtsIMutI CAGTG 3 cut(s) 536, 666, 765
Cac8I GCNNGC 1 cut(s) 681
CciI TCATGA 1 cut(s) 328
Cfr13I GGNCC 1 cut(s) 555
ClaI ATCGAT 1 cut(s) 165
Csp6I GTAC 2 cut(s) 529, 541
CviAII CATG 5 cut(s) 329, 390, 444, 704, 793
CviQI GTAC 2 cut(s) 529, 541
DdeI CTNAG 1 cut(s) 764
DpnI GATC 3 cut(s) 514, 602, 719
DpnII GATC 3 cut(s) 512, 600, 717
DraI TTTAAA 1 cut(s) 570
EaeI YGGCCR 1 cut(s) 302
Eam1104I CTCTTC 1 cut(s) 111
EarI CTCTTC 1 cut(s) 111
Eco130I CCWWGG 1 cut(s) 139
Eco24I GRGCYC 1 cut(s) 508
Eco47I GGWCC 1 cut(s) 555
Eco72I CACGTG 1 cut(s) 272
Eco91I GGTNACC 2 cut(s) 143, 698
EcoO65I GGTNACC 2 cut(s) 143, 698
EcoRII CCWGG 2 cut(s) 64, 508
EcoT14I CCWWGG 1 cut(s) 139
EcoT38I GRGCYC 1 cut(s) 508
ErhI CCWWGG 1 cut(s) 139
FaeI CATG 5 cut(s) 332, 393, 447, 707, 796
FatI CATG 5 cut(s) 328, 389, 443, 703, 792
FauI CCCGC 1 cut(s) 225
FblI GTMKAC 1 cut(s) 789
Fnu4HI GCNGC 5 cut(s) 264, 267, 627, 819, 861
FokI GGATG 1 cut(s) 591
FriOI GRGCYC 1 cut(s) 508
Fsp4HI GCNGC 5 cut(s) 264, 267, 627, 819, 861
GluI GCNGC 5 cut(s) 264, 267, 627, 819, 861
HaeIII GGCC 2 cut(s) 138, 304
Hin1II CATG 5 cut(s) 332, 393, 447, 707, 796
HinfI GANTC 3 cut(s) 61, 755, 849
HphI GGTGA 3 cut(s) 93, 155, 710
Hpy166II GTNNAC 2 cut(s) 536, 790
Hpy188I TCNGA 4 cut(s) 559, 600, 642, 803
Hpy188III TCNNGA 8 cut(s) 58, 93, 113, 287, 329, 418, 752, 853
Hpy8I GTNNAC 2 cut(s) 536, 790
HpyAV CCTTC 2 cut(s) 452, 830
HpyCH4III ACNGT 4 cut(s) 20, 533, 540, 545
HpyCH4IV ACGT 2 cut(s) 118, 271
HpyCH4V TGCA 7 cut(s) 176, 338, 361, 370, 380, 618, 683
HpyF10VI GCNNNNNNNGC 4 cut(s) 215, 272, 367, 632
HpyF3I CTNAG 1 cut(s) 764
HpySE526I ACGT 2 cut(s) 118, 271
Hsp92II CATG 5 cut(s) 332, 393, 447, 707, 796
KpnI GGTACC 1 cut(s) 532
Kzo9I GATC 3 cut(s) 512, 600, 717
LmnI GCTCC 4 cut(s) 82, 511, 640, 839
Lsp1109I GCAGC 3 cut(s) 250, 613, 805
LweI GCATC 2 cut(s) 627, 777
MaeII ACGT 2 cut(s) 118, 271
MaeIII GTNAC 4 cut(s) 20, 143, 433, 698
MalI GATC 3 cut(s) 514, 602, 719
MboI GATC 3 cut(s) 512, 600, 717
MboII GAAGA 8 cut(s) 47, 50, 53, 98, 269, 304, 640, 712
MfeI CAATTG 1 cut(s) 684
MflI RGATCY 2 cut(s) 512, 717
MhlI GDGCHC 1 cut(s) 508
MlsI TGGCCA 1 cut(s) 304
MluCI AATT 8 cut(s) 69, 221, 279, 298, 318, 351, 468, 684
MluNI TGGCCA 1 cut(s) 304
MlyI GAGTC 1 cut(s) 858
Mox20I TGGCCA 1 cut(s) 304
MscI TGGCCA 1 cut(s) 304
MseI TTAA 2 cut(s) 450, 569
Msp20I TGGCCA 1 cut(s) 304
MspR9I CCNGG 2 cut(s) 66, 510
MunI CAATTG 1 cut(s) 684
Mva1269I GAATGC 2 cut(s) 380, 672
MvaI CCWGG 2 cut(s) 66, 510
MwoI GCNNNNNNNGC 4 cut(s) 215, 272, 367, 632
NdeII GATC 3 cut(s) 512, 600, 717
NlaIII CATG 5 cut(s) 332, 393, 447, 707, 796
NlaIV GGNNCC 4 cut(s) 476, 507, 514, 530
NmuCI GTSAC 2 cut(s) 143, 698
NspI RCATGY 1 cut(s) 796
PagI TCATGA 1 cut(s) 328
PciI ACATGT 1 cut(s) 792
PctI GAATGC 2 cut(s) 380, 672
PfeI GAWTC 2 cut(s) 61, 755
PflMI CCANNNNNTGG 1 cut(s) 709
PfoI TCCNGGA 2 cut(s) 64, 508
PkrI GCNGC 5 cut(s) 265, 268, 628, 820, 862
PleI GAGTC 1 cut(s) 857
PmaCI CACGTG 1 cut(s) 272
PmlI CACGTG 1 cut(s) 272
PpsI GAGTC 1 cut(s) 857
Ppu21I YACGTR 1 cut(s) 272
PscI ACATGT 1 cut(s) 792
Psp6I CCWGG 2 cut(s) 64, 508
PspCI CACGTG 1 cut(s) 272
PspEI GGTNACC 2 cut(s) 143, 698
PspGI CCWGG 2 cut(s) 64, 508
PspN4I GGNNCC 4 cut(s) 476, 507, 514, 530
PspPI GGNCC 1 cut(s) 555
PsuI RGATCY 2 cut(s) 512, 717
RsaI GTAC 2 cut(s) 530, 542
RsaNI GTAC 2 cut(s) 529, 541
SaqAI TTAA 2 cut(s) 450, 569
SatI GCNGC 5 cut(s) 264, 267, 627, 819, 861
Sau3AI GATC 3 cut(s) 512, 600, 717
Sau96I GGNCC 1 cut(s) 555
SchI GAGTC 1 cut(s) 858
ScrFI CCNGG 2 cut(s) 66, 510
SduI GDGCHC 1 cut(s) 508
SfaNI GCATC 2 cut(s) 627, 777
SinI GGWCC 1 cut(s) 555
SmlI CTYRAG 2 cut(s) 91, 287
SmoI CTYRAG 2 cut(s) 91, 287
Sse9I AATT 8 cut(s) 69, 221, 279, 298, 318, 351, 468, 684
SsiI CCGC 4 cut(s) 232, 267, 629, 810
StyD4I CCNGG 2 cut(s) 64, 508
StyI CCWWGG 1 cut(s) 139
TaaI ACNGT 4 cut(s) 20, 533, 540, 545
TaiI ACGT 2 cut(s) 121, 274
TaqI TCGA 2 cut(s) 59, 165
TasI AATT 8 cut(s) 69, 221, 279, 298, 318, 351, 468, 684
TauI GCSGC 1 cut(s) 269
TfiI GAWTC 2 cut(s) 61, 755
Tru1I TTAA 2 cut(s) 450, 569
Tru9I TTAA 2 cut(s) 450, 569
TscAI CASTG 3 cut(s) 543, 673, 765
TseFI GTSAC 2 cut(s) 143, 698
TseI GCWGC 4 cut(s) 263, 626, 818, 860
Tsp45I GTSAC 2 cut(s) 143, 698
TspDTI ATGAA 4 cut(s) 344, 357, 484, 718
TspGWI ACGGA 1 cut(s) 265
TspRI CASTG 3 cut(s) 543, 673, 765
Van91I CCANNNNNTGG 1 cut(s) 709
VpaK11BI GGWCC 1 cut(s) 555
XapI RAATTY 1 cut(s) 468
XceI RCATGY 1 cut(s) 796
XcmI CCANNNNNNNNNTGG 1 cut(s) 710
XmiI GTMKAC 1 cut(s) 789
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.