AT2G21750

Egg cell-secreted protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0008373
Physical Location & Seq
Reverse (-)
9283367 .. 9283774
408 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G21750.1

Sequence Viewer

Length: 378 bp
ATGGCTTCTAACACAAGTTTCCTCTTTGTCACCGTCACTCTTCTCCTCGTTCTCAACGTCTCCAGCAGAGCACTCCCGCCCGTGGCGGATTCCACCAACATAGCGGCTAGACTAACCGGAGGAGGACTGATGCAGTGTTGGGATGCACTCTACGAGCTGAAGTCATGTACTAATGAGATCGTTCTCTTCTTTCTCAACGGTGAGACCAAACTCGGCTACGGTTGCTGCAACGCCGTTGATGTCATTACCACTGATTGTTGGCCGGCGATGCTTACTTCTCTTGGCTTTACACTGGAGGAAACCAATGTCCTCCGTGGTTTCTGTCAATCTCCGAACTCCGGCGGTTCTTCTCCAGCTCTTTCCCCTGTCAAACTTTGA

Protein Analysis

125

Amino Acids

13.27

Weight (kDa)

4.61

Isoelectric Point (pI)

44.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Prolamin_like PF05617 45 - 108 3.2e-19 Prolamin-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 77, 86, 104, 342
AcoI YGGCCR 1 cut(s) 260
AcuI CTGAAG 1 cut(s) 179
AfaI GTAC 1 cut(s) 169
AfiI CCNNNNNNNGG 2 cut(s) 82, 338
AluBI AGCT 2 cut(s) 157, 356
AluI AGCT 2 cut(s) 157, 356
Alw21I GWGCWC 1 cut(s) 73
Alw26I GTCTC 2 cut(s) 64, 197
AoxI GGCC 1 cut(s) 260
ApeKI GCWGC 1 cut(s) 225
AsuHPI GGTGA 2 cut(s) 22, 212
Bbv12I GWGCWC 1 cut(s) 73
BbvI GCAGC 1 cut(s) 212
BceAI ACGGC 1 cut(s) 218
BcoDI GTCTC 2 cut(s) 64, 197
BfaI CTAG 1 cut(s) 108
BisI GCNGC 2 cut(s) 105, 226
BlsI GCNGC 2 cut(s) 106, 227
BmsI GCATC 3 cut(s) 120, 133, 258
BpmI CTGGAG 3 cut(s) 46, 314, 336
BsaI GGTCTC 1 cut(s) 197
BsaJI CCNNGG 2 cut(s) 81, 313
BsaWI WCCGGW 1 cut(s) 116
Bsc4I CCNNNNNNNGG 2 cut(s) 82, 338
Bse118I RCCGGY 1 cut(s) 262
Bse1I ACTGG 1 cut(s) 297
BseDI CCNNGG 2 cut(s) 81, 313
BseGI GGATG 1 cut(s) 148
BseLI CCNNNNNNNGG 2 cut(s) 82, 338
BseNI ACTGG 1 cut(s) 297
BseRI GAGGAG 2 cut(s) 35, 135
BseXI GCAGC 1 cut(s) 212
BshFI GGCC 1 cut(s) 262
BsiHKAI GWGCWC 1 cut(s) 73
BsiSI CCGG 3 cut(s) 117, 263, 339
BslI CCNNNNNNNGG 2 cut(s) 82, 338
BsmAI GTCTC 2 cut(s) 64, 197
BsmBI CGTCTC 1 cut(s) 64
BsnI GGCC 1 cut(s) 262
Bso31I GGTCTC 1 cut(s) 197
Bsp1286I GDGCHC 1 cut(s) 73
Bsp143I GATC 1 cut(s) 177
BspACI CCGC 4 cut(s) 77, 86, 104, 342
BspANI GGCC 1 cut(s) 262
BspTNI GGTCTC 1 cut(s) 197
BsrFI RCCGGY 1 cut(s) 262
BsrI ACTGG 1 cut(s) 297
BssAI RCCGGY 1 cut(s) 262
BssECI CCNNGG 2 cut(s) 81, 313
BssMI GATC 1 cut(s) 177
Bst4CI ACNGT 3 cut(s) 34, 200, 221
Bst6I CTCTTC 2 cut(s) 45, 191
BstC8I GCNNGC 1 cut(s) 264
BstDSI CCRYGG 2 cut(s) 81, 313
BstF5I GGATG 1 cut(s) 148
BstKTI GATC 1 cut(s) 180
BstMAI GTCTC 2 cut(s) 64, 197
BstMBI GATC 1 cut(s) 177
BstMWI GCNNNNNNNGC 2 cut(s) 222, 268
BstV1I GCAGC 1 cut(s) 212
BsuRI GGCC 1 cut(s) 262
BtgI CCRYGG 2 cut(s) 81, 313
BtgZI GCGATG 1 cut(s) 281
BtsCI GGATG 1 cut(s) 148
BtsI GCAGTG 1 cut(s) 140
BtsIMutI CAGTG 3 cut(s) 140, 249, 290
Cac8I GCNNGC 1 cut(s) 264
Cfr10I RCCGGY 1 cut(s) 262
Csp6I GTAC 1 cut(s) 168
CviAII CATG 1 cut(s) 165
CviJI RGCY 7 cut(s) 5, 107, 157, 216, 262, 285, 356
CviKI_1 RGCY 7 cut(s) 5, 107, 157, 216, 262, 285, 356
CviQI GTAC 1 cut(s) 168
DpnI GATC 1 cut(s) 179
DpnII GATC 1 cut(s) 177
EaeI YGGCCR 1 cut(s) 260
Eam1104I CTCTTC 2 cut(s) 45, 191
EarI CTCTTC 2 cut(s) 45, 191
EciI GGCGGA 1 cut(s) 101
Eco31I GGTCTC 1 cut(s) 197
Eco57I CTGAAG 1 cut(s) 179
Esp3I CGTCTC 1 cut(s) 64
FaeI CATG 1 cut(s) 168
FaiI YATR 2 cut(s) 101, 166
FatI CATG 1 cut(s) 164
FauI CCCGC 1 cut(s) 84
Fnu4HI GCNGC 2 cut(s) 105, 226
FokI GGATG 1 cut(s) 155
Fsp4HI GCNGC 2 cut(s) 105, 226
FspBI CTAG 1 cut(s) 108
GluI GCNGC 2 cut(s) 105, 226
GsuI CTGGAG 3 cut(s) 46, 314, 336
HaeIII GGCC 1 cut(s) 262
HapII CCGG 3 cut(s) 117, 263, 339
Hin1II CATG 1 cut(s) 168
HinfI GANTC 1 cut(s) 89
HpaII CCGG 3 cut(s) 117, 263, 339
HphI GGTGA 2 cut(s) 22, 212
Hpy188I TCNGA 1 cut(s) 333
HpyCH4III ACNGT 3 cut(s) 34, 200, 221
HpyCH4IV ACGT 1 cut(s) 57
HpyCH4V TGCA 3 cut(s) 133, 146, 228
HpyF10VI GCNNNNNNNGC 2 cut(s) 222, 268
HpySE526I ACGT 1 cut(s) 57
Hsp92II CATG 1 cut(s) 168
KroI GCCGGC 1 cut(s) 262
KroNI GCCGGC 1 cut(s) 264
Kzo9I GATC 1 cut(s) 177
LpnPI CCDG 6 cut(s) 76, 130, 276, 278, 352, 366
Lsp1109I GCAGC 1 cut(s) 212
LweI GCATC 3 cut(s) 120, 133, 258
MaeI CTAG 1 cut(s) 108
MaeII ACGT 1 cut(s) 57
MaeIII GTNAC 2 cut(s) 28, 34
MalI GATC 1 cut(s) 179
MboI GATC 1 cut(s) 177
MboII GAAGA 3 cut(s) 32, 178, 339
MhlI GDGCHC 1 cut(s) 73
MnlI CCTC 6 cut(s) 32, 56, 113, 116, 289, 320
MroNI GCCGGC 1 cut(s) 262
MspI CCGG 3 cut(s) 117, 263, 339
MwoI GCNNNNNNNGC 2 cut(s) 222, 268
NaeI GCCGGC 1 cut(s) 264
NdeII GATC 1 cut(s) 177
NgoMIV GCCGGC 1 cut(s) 262
NlaIII CATG 1 cut(s) 168
NmeAIII GCCGAG 1 cut(s) 192
NmuCI GTSAC 2 cut(s) 28, 34
PcsI WCGNNNNNNNCGW 1 cut(s) 54
PdiI GCCGGC 1 cut(s) 264
PfeI GAWTC 1 cut(s) 89
PkrI GCNGC 2 cut(s) 106, 227
RsaI GTAC 1 cut(s) 169
RsaNI GTAC 1 cut(s) 168
SatI GCNGC 2 cut(s) 105, 226
Sau3AI GATC 1 cut(s) 177
SduI GDGCHC 1 cut(s) 73
SetI ASST 3 cut(s) 60, 159, 358
SfaNI GCATC 3 cut(s) 120, 133, 258
SsiI CCGC 4 cut(s) 77, 86, 104, 342
SspMI CTAG 1 cut(s) 108
TaaI ACNGT 3 cut(s) 34, 200, 221
TaiI ACGT 1 cut(s) 60
TatI WGTACW 1 cut(s) 167
TauI GCSGC 1 cut(s) 107
TfiI GAWTC 1 cut(s) 89
TscAI CASTG 3 cut(s) 140, 256, 297
TseFI GTSAC 2 cut(s) 28, 34
TseI GCWGC 1 cut(s) 225
Tsp45I GTSAC 2 cut(s) 28, 34
TspGWI ACGGA 1 cut(s) 302
TspRI CASTG 3 cut(s) 140, 256, 297
XspI CTAG 1 cut(s) 108
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.