Rh2BG292600

Egg cell-secreted protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0008373
Physical Location & Seq
Reverse (-)
32927896 .. 32928297
402 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG292600.1

Sequence Viewer

Length: 402 bp
ATGGCTGTGAAGAATTTGGTTTTGGTTTCCATGGTGACACTGATCGCATCCCTCATACTGAGTGCAAGTAATGCAACTGCTACTAGGAAAATTTCTGACAAAACGAGCAGTGAAGGCGAAGGCGGGCTAGTGGAATGCGGAAATGCACTGGTGGAGCTGAAATCTTGTTCGCAAGACATTGTTGTGTATTTCCTGAATGGGAAGGCTAATATCGGTCCAGACTGTTGCAAAGCTATCGCCACCATTACACGTCACTGCTGGCCTGCAATGCTTACTTCCCTTGGCTTCACTGCAGAACAAGGTGATGTCTTACGAGGCTACTGTGATGCTGCGGCTGATGTGACCACTACTGCCCCTGCTGCAGCGCCACTTGCTCGAACAACACCTCTTACCAGTTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Prolamin_like

Protein Analysis

133

Amino Acids

13.72

Weight (kDa)

5.71

Isoelectric Point (pI)

33.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Prolamin_like PF05617 45 - 109 5.9e-18 Prolamin-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 123, 138, 332
AcsI RAATTY 2 cut(s) 13, 90
AflIII ACRYGT 1 cut(s) 248
AjiI CACGTC 1 cut(s) 251
AjuI GAANNNNNNNTTGG 1 cut(s) 37
AluBI AGCT 2 cut(s) 157, 233
AluI AGCT 2 cut(s) 157, 233
AoxI GGCC 1 cut(s) 260
ApeKI GCWGC 3 cut(s) 329, 359, 362
ApoI RAATTY 2 cut(s) 13, 90
AspLEI GCGC 1 cut(s) 367
AspS9I GGNCC 1 cut(s) 215
AsuHPI GGTGA 2 cut(s) 46, 314
AvaII GGWCC 1 cut(s) 215
BbvI GCAGC 3 cut(s) 316, 346, 374
BcgI CGANNNNNNTGC 2 cut(s) 217, 251
BfaI CTAG 2 cut(s) 84, 128
BfmI CTRYAG 2 cut(s) 291, 360
BfoI RGCGCY 1 cut(s) 368
BisI GCNGC 4 cut(s) 330, 333, 360, 363
BlsI GCNGC 4 cut(s) 331, 334, 361, 364
Bme18I GGWCC 1 cut(s) 215
BmgBI CACGTC 1 cut(s) 251
BmgT120I GGNCC 1 cut(s) 215
BmsI GCATC 2 cut(s) 56, 316
BsaJI CCNNGG 2 cut(s) 30, 280
Bse1I ACTGG 2 cut(s) 153, 393
Bse3DI GCAATG 1 cut(s) 273
BseDI CCNNGG 2 cut(s) 30, 280
BseGI GGATG 1 cut(s) 47
BseMI GCAATG 1 cut(s) 273
BseMII CTCAG 1 cut(s) 50
BseNI ACTGG 2 cut(s) 153, 393
BseXI GCAGC 3 cut(s) 316, 346, 374
BshFI GGCC 1 cut(s) 262
BsmI GAATGC 1 cut(s) 140
BsnI GGCC 1 cut(s) 262
Bsp143I GATC 1 cut(s) 42
Bsp19I CCATGG 1 cut(s) 30
BspACI CCGC 3 cut(s) 123, 138, 332
BspANI GGCC 1 cut(s) 262
BspCNI CTCAG 1 cut(s) 51
BspMAI CTGCAG 2 cut(s) 295, 364
BsrDI GCAATG 1 cut(s) 273
BsrI ACTGG 2 cut(s) 153, 393
BssECI CCNNGG 2 cut(s) 30, 280
BssMI GATC 1 cut(s) 42
BssT1I CCWWGG 2 cut(s) 30, 280
Bst4CI ACNGT 2 cut(s) 224, 323
BstAPI GCANNNNNTGC 1 cut(s) 71
BstC8I GCNNGC 3 cut(s) 125, 260, 264
BstDEI CTNAG 1 cut(s) 59
BstDSI CCRYGG 1 cut(s) 30
BstF5I GGATG 1 cut(s) 47
BstH2I RGCGCY 1 cut(s) 368
BstHHI GCGC 1 cut(s) 367
BstKTI GATC 1 cut(s) 45
BstMBI GATC 1 cut(s) 42
BstMWI GCNNNNNNNGC 5 cut(s) 71, 114, 268, 359, 371
BstSFI CTRYAG 2 cut(s) 291, 360
BstV1I GCAGC 3 cut(s) 316, 346, 374
BsuRI GGCC 1 cut(s) 262
BtgI CCRYGG 1 cut(s) 30
BtrI CACGTC 1 cut(s) 251
BtsCI GGATG 1 cut(s) 47
BtsI GCAGTG 3 cut(s) 115, 253, 288
BtsIMutI CAGTG 5 cut(s) 38, 115, 146, 253, 288
Cac8I GCNNGC 3 cut(s) 125, 260, 264
CfoI GCGC 1 cut(s) 367
Cfr13I GGNCC 1 cut(s) 215
CviAII CATG 1 cut(s) 31
CviJI RGCY 9 cut(s) 5, 127, 157, 206, 233, 262, 285, 318, 335
CviKI_1 RGCY 9 cut(s) 5, 127, 157, 206, 233, 262, 285, 318, 335
DdeI CTNAG 1 cut(s) 59
DpnI GATC 1 cut(s) 44
DpnII GATC 1 cut(s) 42
Eco130I CCWWGG 2 cut(s) 30, 280
Eco47I GGWCC 1 cut(s) 215
EcoT14I CCWWGG 2 cut(s) 30, 280
ErhI CCWWGG 2 cut(s) 30, 280
FaeI CATG 1 cut(s) 34
FaiI YATR 2 cut(s) 32, 56
FatI CATG 1 cut(s) 30
FauI CCCGC 1 cut(s) 116
Fnu4HI GCNGC 4 cut(s) 330, 333, 360, 363
FokI GGATG 1 cut(s) 34
Fsp4HI GCNGC 4 cut(s) 330, 333, 360, 363
FspBI CTAG 2 cut(s) 84, 128
GlaI GCGC 1 cut(s) 366
GluI GCNGC 4 cut(s) 330, 333, 360, 363
HaeII RGCGCY 1 cut(s) 368
HaeIII GGCC 1 cut(s) 262
HhaI GCGC 1 cut(s) 367
Hin1II CATG 1 cut(s) 34
Hin6I GCGC 1 cut(s) 365
HinP1I GCGC 1 cut(s) 365
HphI GGTGA 2 cut(s) 46, 314
Hpy188I TCNGA 1 cut(s) 97
Hpy188III TCNNGA 2 cut(s) 193, 218
HpyAV CCTTC 3 cut(s) 107, 113, 196
HpyCH4III ACNGT 2 cut(s) 224, 323
HpyCH4IV ACGT 1 cut(s) 250
HpyCH4V TGCA 7 cut(s) 65, 74, 146, 228, 266, 293, 362
HpyF10VI GCNNNNNNNGC 5 cut(s) 71, 114, 268, 359, 371
HpyF3I CTNAG 1 cut(s) 59
HpySE526I ACGT 1 cut(s) 250
Hsp92II CATG 1 cut(s) 34
HspAI GCGC 1 cut(s) 365
Kzo9I GATC 1 cut(s) 42
LmnI GCTCC 1 cut(s) 154
LpnPI CCDG 6 cut(s) 134, 206, 231, 244, 276, 369
Lsp1109I GCAGC 3 cut(s) 316, 346, 374
LweI GCATC 2 cut(s) 56, 316
MaeI CTAG 2 cut(s) 84, 128
MaeII ACGT 1 cut(s) 250
MaeIII GTNAC 3 cut(s) 34, 251, 340
MalI GATC 1 cut(s) 44
MboI GATC 1 cut(s) 42
MboII GAAGA 1 cut(s) 22
MluCI AATT 2 cut(s) 13, 90
MnlI CCTC 3 cut(s) 62, 308, 396
MslI CAYNNNNRTG 1 cut(s) 182
Mva1269I GAATGC 1 cut(s) 140
MwoI GCNNNNNNNGC 5 cut(s) 71, 114, 268, 359, 371
NcoI CCATGG 1 cut(s) 30
NdeII GATC 1 cut(s) 42
NlaIII CATG 1 cut(s) 34
NmuCI GTSAC 3 cut(s) 34, 251, 340
PctI GAATGC 1 cut(s) 140
PkrI GCNGC 4 cut(s) 331, 334, 361, 364
PspPI GGNCC 1 cut(s) 215
PstI CTGCAG 2 cut(s) 295, 364
RseI CAYNNNNRTG 1 cut(s) 182
SatI GCNGC 4 cut(s) 330, 333, 360, 363
Sau3AI GATC 1 cut(s) 42
Sau96I GGNCC 1 cut(s) 215
SetI ASST 5 cut(s) 159, 235, 253, 304, 388
SfaNI GCATC 2 cut(s) 56, 316
SfcI CTRYAG 2 cut(s) 291, 360
SinI GGWCC 1 cut(s) 215
SmiMI CAYNNNNRTG 1 cut(s) 182
Sse9I AATT 2 cut(s) 13, 90
SsiI CCGC 3 cut(s) 123, 138, 332
SspMI CTAG 2 cut(s) 84, 128
StyI CCWWGG 2 cut(s) 30, 280
TaaI ACNGT 2 cut(s) 224, 323
TaiI ACGT 1 cut(s) 253
TaqI TCGA 1 cut(s) 376
TaqII GACCGA 1 cut(s) 203
TasI AATT 2 cut(s) 13, 90
TauI GCSGC 1 cut(s) 335
TscAI CASTG 5 cut(s) 45, 115, 153, 260, 295
TseFI GTSAC 3 cut(s) 34, 251, 340
TseI GCWGC 3 cut(s) 329, 359, 362
Tsp45I GTSAC 3 cut(s) 34, 251, 340
TspRI CASTG 5 cut(s) 45, 115, 153, 260, 295
VpaK11BI GGWCC 1 cut(s) 215
XapI RAATTY 2 cut(s) 13, 90
XspI CTAG 2 cut(s) 84, 128
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.