Rroxscaffold_2G00124100

Egg cell-secreted protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0008373
Physical Location & Seq
Forward (+)
58220128 .. 58220499
372 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00124100.1

Sequence Viewer

Length: 372 bp
ATGGTGACACTGATCGCATCCCTCATACTGAGTGCAAGTAATGCAACTGCTACTAGGAAAATTTCTGACAAAACAAGCGGTGAAGGCGAAGGCGGGCTAGTGGAATGCGGAAATGCACTGGTGGAGCTGAAATCTTGCTCGCAAGAGATTGTTGTGTATTTCCTGAATGGGAAGGCTAATATCGGTCCAGACTGTTGCAAAGCTATCGCCACCATTACACGTCACTGCTGGCCTGCAATGCTTACTTCCCTTGGCTTCACTGCAGAACAAGGTGACGTCTTACGAGGCTACTGTGATGCTGCGGCGGCTGTGACTACTACTGCTCCTGCTGCAGCGCCACTTGCTCGTACAACACCTCTTACCAGTTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Prolamin_like

Protein Analysis

123

Amino Acids

12.61

Weight (kDa)

5.71

Isoelectric Point (pI)

33.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Prolamin_like PF05617 35 - 99 1.9e-18 Prolamin-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 279
AciI CCGC 5 cut(s) 78, 93, 108, 302, 305
AcsI RAATTY 1 cut(s) 60
AcyI GRCGYC 1 cut(s) 276
AfaI GTAC 1 cut(s) 349
AflIII ACRYGT 1 cut(s) 218
AjiI CACGTC 1 cut(s) 221
AluBI AGCT 2 cut(s) 127, 203
AluI AGCT 2 cut(s) 127, 203
AoxI GGCC 1 cut(s) 230
ApeKI GCWGC 3 cut(s) 299, 329, 332
ApoI RAATTY 1 cut(s) 60
AspLEI GCGC 1 cut(s) 337
AspS9I GGNCC 1 cut(s) 185
AsuHPI GGTGA 3 cut(s) 16, 92, 284
AvaII GGWCC 1 cut(s) 185
BbvI GCAGC 3 cut(s) 286, 316, 344
BcgI CGANNNNNNTGC 2 cut(s) 187, 221
BfaI CTAG 2 cut(s) 54, 98
BfmI CTRYAG 2 cut(s) 261, 330
BfoI RGCGCY 1 cut(s) 338
BisI GCNGC 5 cut(s) 300, 303, 306, 330, 333
BlsI GCNGC 5 cut(s) 301, 304, 307, 331, 334
Bme18I GGWCC 1 cut(s) 185
BmgBI CACGTC 1 cut(s) 221
BmgT120I GGNCC 1 cut(s) 185
BmsI GCATC 2 cut(s) 26, 286
BsaHI GRCGYC 1 cut(s) 276
BsaJI CCNNGG 1 cut(s) 250
BsaXI ACNNNNNCTCC 2 cut(s) 307, 337
Bse1I ACTGG 2 cut(s) 123, 363
Bse3DI GCAATG 1 cut(s) 243
BseDI CCNNGG 1 cut(s) 250
BseGI GGATG 1 cut(s) 17
BseMI GCAATG 1 cut(s) 243
BseMII CTCAG 1 cut(s) 20
BseNI ACTGG 2 cut(s) 123, 363
BseXI GCAGC 3 cut(s) 286, 316, 344
BshFI GGCC 1 cut(s) 232
BsmI GAATGC 1 cut(s) 110
BsnI GGCC 1 cut(s) 232
Bsp143I GATC 1 cut(s) 12
BspACI CCGC 5 cut(s) 78, 93, 108, 302, 305
BspANI GGCC 1 cut(s) 232
BspCNI CTCAG 1 cut(s) 21
BspMAI CTGCAG 2 cut(s) 265, 334
BsrDI GCAATG 1 cut(s) 243
BsrI ACTGG 2 cut(s) 123, 363
BssECI CCNNGG 1 cut(s) 250
BssMI GATC 1 cut(s) 12
BssNI GRCGYC 1 cut(s) 276
BssT1I CCWWGG 1 cut(s) 250
Bst4CI ACNGT 2 cut(s) 194, 293
BstACI GRCGYC 1 cut(s) 276
BstAPI GCANNNNNTGC 1 cut(s) 41
BstC8I GCNNGC 4 cut(s) 95, 140, 230, 234
BstDEI CTNAG 1 cut(s) 29
BstF5I GGATG 1 cut(s) 17
BstH2I RGCGCY 1 cut(s) 338
BstHHI GCGC 1 cut(s) 337
BstKTI GATC 1 cut(s) 15
BstMBI GATC 1 cut(s) 12
BstMWI GCNNNNNNNGC 6 cut(s) 41, 84, 238, 305, 329, 341
BstSFI CTRYAG 2 cut(s) 261, 330
BstV1I GCAGC 3 cut(s) 286, 316, 344
BsuRI GGCC 1 cut(s) 232
BtrI CACGTC 1 cut(s) 221
BtsCI GGATG 1 cut(s) 17
BtsI GCAGTG 2 cut(s) 223, 258
BtsIMutI CAGTG 4 cut(s) 8, 116, 223, 258
Cac8I GCNNGC 4 cut(s) 95, 140, 230, 234
CfoI GCGC 1 cut(s) 337
Cfr13I GGNCC 1 cut(s) 185
Csp6I GTAC 1 cut(s) 348
CviJI RGCY 8 cut(s) 97, 127, 176, 203, 232, 255, 288, 308
CviKI_1 RGCY 8 cut(s) 97, 127, 176, 203, 232, 255, 288, 308
CviQI GTAC 1 cut(s) 348
DdeI CTNAG 1 cut(s) 29
DpnI GATC 1 cut(s) 14
DpnII GATC 1 cut(s) 12
Eco130I CCWWGG 1 cut(s) 250
Eco47I GGWCC 1 cut(s) 185
EcoT14I CCWWGG 1 cut(s) 250
ErhI CCWWGG 1 cut(s) 250
FaiI YATR 1 cut(s) 26
FauI CCCGC 1 cut(s) 86
Fnu4HI GCNGC 5 cut(s) 300, 303, 306, 330, 333
FokI GGATG 1 cut(s) 4
Fsp4HI GCNGC 5 cut(s) 300, 303, 306, 330, 333
FspBI CTAG 2 cut(s) 54, 98
GlaI GCGC 1 cut(s) 336
GluI GCNGC 5 cut(s) 300, 303, 306, 330, 333
HaeII RGCGCY 1 cut(s) 338
HaeIII GGCC 1 cut(s) 232
HhaI GCGC 1 cut(s) 337
Hin1I GRCGYC 1 cut(s) 276
Hin6I GCGC 1 cut(s) 335
HinP1I GCGC 1 cut(s) 335
HphI GGTGA 3 cut(s) 16, 92, 284
Hpy188I TCNGA 1 cut(s) 67
Hpy188III TCNNGA 2 cut(s) 163, 188
HpyAV CCTTC 3 cut(s) 77, 83, 166
HpyCH4III ACNGT 2 cut(s) 194, 293
HpyCH4IV ACGT 2 cut(s) 220, 276
HpyCH4V TGCA 7 cut(s) 35, 44, 116, 198, 236, 263, 332
HpyF10VI GCNNNNNNNGC 6 cut(s) 41, 84, 238, 305, 329, 341
HpyF3I CTNAG 1 cut(s) 29
HpySE526I ACGT 2 cut(s) 220, 276
Hsp92I GRCGYC 1 cut(s) 276
HspAI GCGC 1 cut(s) 335
Kzo9I GATC 1 cut(s) 12
LmnI GCTCC 2 cut(s) 124, 328
LpnPI CCDG 6 cut(s) 104, 176, 201, 214, 246, 339
Lsp1109I GCAGC 3 cut(s) 286, 316, 344
LweI GCATC 2 cut(s) 26, 286
MaeI CTAG 2 cut(s) 54, 98
MaeII ACGT 2 cut(s) 220, 276
MaeIII GTNAC 4 cut(s) 4, 221, 272, 310
MalI GATC 1 cut(s) 14
MboI GATC 1 cut(s) 12
MluCI AATT 1 cut(s) 60
MnlI CCTC 3 cut(s) 32, 278, 366
Mva1269I GAATGC 1 cut(s) 110
MwoI GCNNNNNNNGC 6 cut(s) 41, 84, 238, 305, 329, 341
NdeII GATC 1 cut(s) 12
NmuCI GTSAC 4 cut(s) 4, 221, 272, 310
PctI GAATGC 1 cut(s) 110
PkrI GCNGC 5 cut(s) 301, 304, 307, 331, 334
PspPI GGNCC 1 cut(s) 185
PstI CTGCAG 2 cut(s) 265, 334
RsaI GTAC 1 cut(s) 349
RsaNI GTAC 1 cut(s) 348
SatI GCNGC 5 cut(s) 300, 303, 306, 330, 333
Sau3AI GATC 1 cut(s) 12
Sau96I GGNCC 1 cut(s) 185
SetI ASST 6 cut(s) 129, 205, 223, 274, 279, 358
SfaNI GCATC 2 cut(s) 26, 286
SfcI CTRYAG 2 cut(s) 261, 330
SinI GGWCC 1 cut(s) 185
Sse9I AATT 1 cut(s) 60
SsiI CCGC 5 cut(s) 78, 93, 108, 302, 305
SspMI CTAG 2 cut(s) 54, 98
StyI CCWWGG 1 cut(s) 250
TaaI ACNGT 2 cut(s) 194, 293
TaiI ACGT 2 cut(s) 223, 279
TaqII GACCGA 1 cut(s) 173
TasI AATT 1 cut(s) 60
TauI GCSGC 2 cut(s) 305, 308
TscAI CASTG 4 cut(s) 15, 123, 230, 265
TseFI GTSAC 4 cut(s) 4, 221, 272, 310
TseI GCWGC 3 cut(s) 299, 329, 332
Tsp45I GTSAC 4 cut(s) 4, 221, 272, 310
TspRI CASTG 4 cut(s) 15, 123, 230, 265
VpaK11BI GGWCC 1 cut(s) 185
XapI RAATTY 1 cut(s) 60
XspI CTAG 2 cut(s) 54, 98
ZraI GACGTC 1 cut(s) 277
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.