RchiOBHm_Chr2g0119721

Egg cell-secreted protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0008373
Physical Location & Seq
Reverse (-)
32270886 .. 32271293
408 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ49241

Sequence Viewer

Length: 408 bp
ATGGCTTTGAAGAATTTGGTTTTTCTTTCCATGGTGACACTGATCGCATCCCTCATAATGAGTGCAAGTAATGCAACTGCTACCAGGAAAATTTCTGCAGGGATCAAAACGAGCGGTGAAGGCGAAGGCGGGCTAGTGGAATGCGGAAATGCGCTGGAGGAGCTGAAATCTTGCTCGCAAGAGATTGTTGTGTATTTCCTGAATGGGAAGGCTAATATCGGCCCAGACTGTTGCAAAGCTATCGCCACCATTACACGTCACTGCTGGCCTGCAATGCTTACTTCCCTTGGCTTCACTGCAGAACAAGGTGACATCTTACGAGGCTACTGTGATGCTGCGGCTGCCGTGACTACTACTGCTCCTGCTGCGGCGCCACTTGCTCGTACAACACCTCTTACCAGTTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Prolamin_like

Protein Analysis

135

Amino Acids

13.92

Weight (kDa)

6.54

Isoelectric Point (pI)

36.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Prolamin_like PF05617 47 - 111 1.2e-18 Prolamin-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 370
AccBSI CCGCTC 1 cut(s) 114
AciI CCGC 5 cut(s) 114, 129, 144, 338, 368
AclWI GGATC 1 cut(s) 110
AcsI RAATTY 2 cut(s) 13, 90
AcyI GRCGYC 1 cut(s) 371
AfaI GTAC 1 cut(s) 385
AflIII ACRYGT 1 cut(s) 254
AgsI TTSAA 1 cut(s) 10
AjiI CACGTC 1 cut(s) 257
AjnI CCWGG 1 cut(s) 83
AluBI AGCT 2 cut(s) 163, 239
AluI AGCT 2 cut(s) 163, 239
AlwI GGATC 1 cut(s) 110
AoxI GGCC 2 cut(s) 220, 266
ApeKI GCWGC 3 cut(s) 335, 341, 365
ApoI RAATTY 2 cut(s) 13, 90
AspLEI GCGC 2 cut(s) 154, 373
AspS9I GGNCC 1 cut(s) 221
AsuHPI GGTGA 3 cut(s) 46, 128, 320
BanI GGYRCC 1 cut(s) 370
BbvI GCAGC 3 cut(s) 322, 328, 352
BceAI ACGGC 1 cut(s) 329
BcgI CGANNNNNNTGC 2 cut(s) 223, 257
BciT130I CCWGG 1 cut(s) 85
BfaI CTAG 1 cut(s) 134
BfmI CTRYAG 2 cut(s) 96, 297
BfoI RGCGCY 1 cut(s) 374
BisI GCNGC 5 cut(s) 336, 339, 342, 366, 369
BlsI GCNGC 5 cut(s) 337, 340, 343, 367, 370
Bme1390I CCNGG 1 cut(s) 85
BmgBI CACGTC 1 cut(s) 257
BmgT120I GGNCC 1 cut(s) 221
BmiI GGNNCC 1 cut(s) 372
BmrFI CCNGG 1 cut(s) 85
BmsI GCATC 2 cut(s) 56, 322
BpmI CTGGAG 1 cut(s) 176
BsaHI GRCGYC 1 cut(s) 371
BsaJI CCNNGG 2 cut(s) 30, 286
BsaXI ACNNNNNCTCC 2 cut(s) 343, 373
Bse1I ACTGG 1 cut(s) 399
Bse3DI GCAATG 1 cut(s) 279
BseBI CCWGG 1 cut(s) 85
BseDI CCNNGG 2 cut(s) 30, 286
BseGI GGATG 1 cut(s) 47
BseMI GCAATG 1 cut(s) 279
BseNI ACTGG 1 cut(s) 399
BseRI GAGGAG 1 cut(s) 173
BseXI GCAGC 3 cut(s) 322, 328, 352
BshFI GGCC 2 cut(s) 222, 268
BshNI GGYRCC 1 cut(s) 370
BsmI GAATGC 1 cut(s) 146
BsnI GGCC 2 cut(s) 222, 268
Bsp143I GATC 2 cut(s) 42, 102
Bsp19I CCATGG 1 cut(s) 30
BspACI CCGC 5 cut(s) 114, 129, 144, 338, 368
BspANI GGCC 2 cut(s) 222, 268
BspLI GGNNCC 1 cut(s) 372
BspMAI CTGCAG 2 cut(s) 100, 301
BspPI GGATC 1 cut(s) 110
BspT107I GGYRCC 1 cut(s) 370
BsrBI CCGCTC 1 cut(s) 114
BsrDI GCAATG 1 cut(s) 279
BsrI ACTGG 1 cut(s) 399
BssECI CCNNGG 2 cut(s) 30, 286
BssMI GATC 2 cut(s) 42, 102
BssNI GRCGYC 1 cut(s) 371
BssT1I CCWWGG 2 cut(s) 30, 286
Bst2UI CCWGG 1 cut(s) 85
Bst4CI ACNGT 2 cut(s) 230, 329
BstACI GRCGYC 1 cut(s) 371
BstAPI GCANNNNNTGC 1 cut(s) 71
BstC8I GCNNGC 4 cut(s) 131, 176, 266, 270
BstDSI CCRYGG 1 cut(s) 30
BstF5I GGATG 1 cut(s) 47
BstH2I RGCGCY 1 cut(s) 374
BstHHI GCGC 2 cut(s) 154, 373
BstKTI GATC 2 cut(s) 45, 105
BstMBI GATC 2 cut(s) 42, 102
BstMWI GCNNNNNNNGC 7 cut(s) 71, 120, 160, 274, 341, 365, 377
BstNI CCWGG 1 cut(s) 85
BstSCI CCNGG 1 cut(s) 83
BstSFI CTRYAG 2 cut(s) 96, 297
BstV1I GCAGC 3 cut(s) 322, 328, 352
BsuRI GGCC 2 cut(s) 222, 268
BtgI CCRYGG 1 cut(s) 30
BtrI CACGTC 1 cut(s) 257
BtsCI GGATG 1 cut(s) 47
BtsI GCAGTG 2 cut(s) 259, 294
BtsIMutI CAGTG 3 cut(s) 38, 259, 294
Cac8I GCNNGC 4 cut(s) 131, 176, 266, 270
CfoI GCGC 2 cut(s) 154, 373
Cfr13I GGNCC 1 cut(s) 221
Csp6I GTAC 1 cut(s) 384
CviAII CATG 1 cut(s) 31
CviQI GTAC 1 cut(s) 384
DinI GGCGCC 1 cut(s) 372
DpnI GATC 2 cut(s) 44, 104
DpnII GATC 2 cut(s) 42, 102
Eco130I CCWWGG 2 cut(s) 30, 286
EcoRII CCWGG 1 cut(s) 83
EcoT14I CCWWGG 2 cut(s) 30, 286
EgeI GGCGCC 1 cut(s) 372
EheI GGCGCC 1 cut(s) 372
ErhI CCWWGG 2 cut(s) 30, 286
FaeI CATG 1 cut(s) 34
FaiI YATR 2 cut(s) 32, 56
FatI CATG 1 cut(s) 30
FauI CCCGC 1 cut(s) 122
Fnu4HI GCNGC 5 cut(s) 336, 339, 342, 366, 369
FokI GGATG 1 cut(s) 34
Fsp4HI GCNGC 5 cut(s) 336, 339, 342, 366, 369
FspBI CTAG 1 cut(s) 134
GlaI GCGC 2 cut(s) 153, 372
GluI GCNGC 5 cut(s) 336, 339, 342, 366, 369
GsuI CTGGAG 1 cut(s) 176
HaeII RGCGCY 1 cut(s) 374
HaeIII GGCC 2 cut(s) 222, 268
HhaI GCGC 2 cut(s) 154, 373
Hin1I GRCGYC 1 cut(s) 371
Hin1II CATG 1 cut(s) 34
Hin6I GCGC 2 cut(s) 152, 371
HinP1I GCGC 2 cut(s) 152, 371
HphI GGTGA 3 cut(s) 46, 128, 320
Hpy188III TCNNGA 1 cut(s) 199
HpyAV CCTTC 3 cut(s) 113, 119, 202
HpyCH4III ACNGT 2 cut(s) 230, 329
HpyCH4IV ACGT 1 cut(s) 256
HpyCH4V TGCA 6 cut(s) 65, 74, 98, 234, 272, 299
HpyF10VI GCNNNNNNNGC 7 cut(s) 71, 120, 160, 274, 341, 365, 377
HpySE526I ACGT 1 cut(s) 256
Hsp92I GRCGYC 1 cut(s) 371
Hsp92II CATG 1 cut(s) 34
HspAI GCGC 2 cut(s) 152, 371
KasI GGCGCC 1 cut(s) 370
Kzo9I GATC 2 cut(s) 42, 102
LmnI GCTCC 2 cut(s) 160, 364
LpnPI CCDG 9 cut(s) 70, 84, 97, 140, 212, 237, 250, 282, 375
Lsp1109I GCAGC 3 cut(s) 322, 328, 352
LweI GCATC 2 cut(s) 56, 322
MaeI CTAG 1 cut(s) 134
MaeII ACGT 1 cut(s) 256
MaeIII GTNAC 4 cut(s) 34, 257, 308, 346
MalI GATC 2 cut(s) 44, 104
MbiI CCGCTC 1 cut(s) 114
MboI GATC 2 cut(s) 42, 102
MboII GAAGA 1 cut(s) 22
MluCI AATT 2 cut(s) 13, 90
Mly113I GGCGCC 1 cut(s) 371
MnlI CCTC 4 cut(s) 62, 151, 314, 402
MspR9I CCNGG 1 cut(s) 85
Mva1269I GAATGC 1 cut(s) 146
MvaI CCWGG 1 cut(s) 85
MwoI GCNNNNNNNGC 7 cut(s) 71, 120, 160, 274, 341, 365, 377
NarI GGCGCC 1 cut(s) 371
NcoI CCATGG 1 cut(s) 30
NdeII GATC 2 cut(s) 42, 102
NlaIII CATG 1 cut(s) 34
NlaIV GGNNCC 1 cut(s) 372
NmuCI GTSAC 4 cut(s) 34, 257, 308, 346
PctI GAATGC 1 cut(s) 146
PkrI GCNGC 5 cut(s) 337, 340, 343, 367, 370
PluTI GGCGCC 1 cut(s) 374
Psp6I CCWGG 1 cut(s) 83
PspGI CCWGG 1 cut(s) 83
PspN4I GGNNCC 1 cut(s) 372
PspPI GGNCC 1 cut(s) 221
PstI CTGCAG 2 cut(s) 100, 301
RsaI GTAC 1 cut(s) 385
RsaNI GTAC 1 cut(s) 384
SatI GCNGC 5 cut(s) 336, 339, 342, 366, 369
Sau3AI GATC 2 cut(s) 42, 102
Sau96I GGNCC 1 cut(s) 221
ScrFI CCNGG 1 cut(s) 85
SetI ASST 5 cut(s) 165, 241, 259, 310, 394
SfaNI GCATC 2 cut(s) 56, 322
SfcI CTRYAG 2 cut(s) 96, 297
SfoI GGCGCC 1 cut(s) 372
Sse9I AATT 2 cut(s) 13, 90
SsiI CCGC 5 cut(s) 114, 129, 144, 338, 368
SspDI GGCGCC 1 cut(s) 370
SspMI CTAG 1 cut(s) 134
StyD4I CCNGG 1 cut(s) 83
StyI CCWWGG 2 cut(s) 30, 286
TaaI ACNGT 2 cut(s) 230, 329
TaiI ACGT 1 cut(s) 259
TasI AATT 2 cut(s) 13, 90
TauI GCSGC 2 cut(s) 341, 371
TscAI CASTG 3 cut(s) 45, 266, 301
TseFI GTSAC 4 cut(s) 34, 257, 308, 346
TseI GCWGC 3 cut(s) 335, 341, 365
Tsp45I GTSAC 4 cut(s) 34, 257, 308, 346
TspRI CASTG 3 cut(s) 45, 266, 301
XapI RAATTY 2 cut(s) 13, 90
XspI CTAG 1 cut(s) 134
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.