MD01G1001000.v1.1

Egg cell-secreted protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0008373
Physical Location & Seq
Forward (+)
264113 .. 264565
453 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1001000.v1.1.491

Sequence Viewer

Length: 453 bp
ATGGCTTCCCTAAACGTGGCTTTCCCGGTGGCAGTATGCCTAATATTCTTGGGTGCAGACCACGCACTTGCAGCAAGAAACGTGATTAATCCCATGCAGCCAGGCTACAACATCCCCATCGCGGCAAGGATCAAAACTGGAAACGAAGGGGATGGTAGACTAGCGAATTGCTGGAATGCGCTTGTGGAGCTGAAATCATGCTCCAATGAGGTGGTTCTGTTTTTCCTGAATGGGCAGGCTGATATAGGGCCCGACTGTTGCAAAGCCATTGCCACTATTACACGCCACTGCTGGCCTGCAATGCTCACTTCGATTGGTTTCACGGCTGAGGAGGGTAACATTTTGCGAGGCTACTGTGATGCCGCTGCATCTGCTACTAGTACTAATTCCTCCGCCCCTTCTTCACCACCTCTTGCTGCCGCCGCTACCACGCCTGTTCCTCCCGCCCACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Prolamin_like

Protein Analysis

151

Amino Acids

15.52

Weight (kDa)

5.82

Isoelectric Point (pI)

40.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Prolamin_like PF05617 56 - 120 1.8e-20 Prolamin-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 157
AccII CGCG 1 cut(s) 122
AciI CCGC 6 cut(s) 122, 363, 393, 420, 423, 444
AclWI GGATC 1 cut(s) 137
AfaI GTAC 1 cut(s) 382
AfiI CCNNNNNNNGG 2 cut(s) 16, 121
AhlI ACTAGT 1 cut(s) 377
AjnI CCWGG 1 cut(s) 100
AluBI AGCT 1 cut(s) 190
AluI AGCT 1 cut(s) 190
AlwI GGATC 1 cut(s) 137
AoxI GGCC 2 cut(s) 248, 293
ApaI GGGCCC 1 cut(s) 252
ApeKI GCWGC 4 cut(s) 71, 97, 365, 416
AseI ATTAAT 1 cut(s) 87
AspLEI GCGC 1 cut(s) 181
AspS9I GGNCC 2 cut(s) 248, 249
AsuC2I CCSGG 1 cut(s) 26
AsuHPI GGTGA 1 cut(s) 396
BaeGI GKGCMC 1 cut(s) 252
BanII GRGCYC 1 cut(s) 252
BbvCI CCTCAGC 1 cut(s) 327
BbvI GCAGC 4 cut(s) 83, 109, 352, 403
BccI CCATC 2 cut(s) 125, 146
BceAI ACGGC 1 cut(s) 339
BciT130I CCWGG 1 cut(s) 102
BcnI CCSGG 1 cut(s) 26
BcuI ACTAGT 1 cut(s) 377
BfaI CTAG 2 cut(s) 161, 378
BisI GCNGC 8 cut(s) 72, 98, 123, 363, 366, 417, 420, 423
BlsI GCNGC 8 cut(s) 73, 99, 124, 364, 367, 418, 421, 424
BmcAI AGTACT 1 cut(s) 382
Bme1390I CCNGG 2 cut(s) 26, 102
BmgT120I GGNCC 2 cut(s) 248, 249
BmiI GGNNCC 1 cut(s) 250
BmrFI CCNGG 2 cut(s) 26, 102
BmsI GCATC 2 cut(s) 349, 377
Bpu10I CCTNAGC 1 cut(s) 327
BpuMI CCSGG 1 cut(s) 26
Bsc4I CCNNNNNNNGG 2 cut(s) 16, 121
Bse1I ACTGG 1 cut(s) 142
Bse3DI GCAATG 2 cut(s) 267, 306
BseBI CCWGG 1 cut(s) 102
BseGI GGATG 2 cut(s) 111, 157
BseLI CCNNNNNNNGG 2 cut(s) 16, 121
BseMI GCAATG 2 cut(s) 267, 306
BseMII CTCAG 1 cut(s) 318
BseNI ACTGG 1 cut(s) 142
BseRI GAGGAG 1 cut(s) 344
BseSI GKGCMC 1 cut(s) 252
BseXI GCAGC 4 cut(s) 83, 109, 352, 403
BsgI GTGCAG 1 cut(s) 75
Bsh1236I CGCG 1 cut(s) 122
BshFI GGCC 2 cut(s) 250, 295
BsiSI CCGG 1 cut(s) 26
BslI CCNNNNNNNGG 2 cut(s) 16, 121
BsmI GAATGC 1 cut(s) 181
BsnI GGCC 2 cut(s) 250, 295
Bsp120I GGGCCC 1 cut(s) 248
Bsp1286I GDGCHC 1 cut(s) 252
Bsp143I GATC 1 cut(s) 129
BspACI CCGC 6 cut(s) 122, 363, 393, 420, 423, 444
BspANI GGCC 2 cut(s) 250, 295
BspCNI CTCAG 1 cut(s) 319
BspFNI CGCG 1 cut(s) 122
BspLI GGNNCC 1 cut(s) 250
BspPI GGATC 1 cut(s) 137
BsrDI GCAATG 2 cut(s) 267, 306
BsrI ACTGG 1 cut(s) 142
BssMI GATC 1 cut(s) 129
Bst2UI CCWGG 1 cut(s) 102
Bst4CI ACNGT 2 cut(s) 257, 356
BstC8I GCNNGC 3 cut(s) 237, 293, 297
BstDEI CTNAG 1 cut(s) 327
BstF5I GGATG 2 cut(s) 111, 157
BstFNI CGCG 1 cut(s) 122
BstHHI GCGC 1 cut(s) 181
BstKTI GATC 1 cut(s) 132
BstMBI GATC 1 cut(s) 129
BstMWI GCNNNNNNNGC 6 cut(s) 62, 71, 187, 301, 371, 422
BstNI CCWGG 1 cut(s) 102
BstSCI CCNGG 2 cut(s) 24, 100
BstSLI GKGCMC 1 cut(s) 252
BstUI CGCG 1 cut(s) 122
BstV1I GCAGC 4 cut(s) 83, 109, 352, 403
BstXI CCANNNNNNTGG 1 cut(s) 211
BsuRI GGCC 2 cut(s) 250, 295
BtgZI GCGATG 1 cut(s) 103
BtsCI GGATG 2 cut(s) 111, 157
BtsI GCAGTG 1 cut(s) 286
BtsIMutI CAGTG 1 cut(s) 286
Cac8I GCNNGC 3 cut(s) 237, 293, 297
CfoI GCGC 1 cut(s) 181
Cfr13I GGNCC 2 cut(s) 248, 249
Csp6I GTAC 1 cut(s) 381
CviAII CATG 2 cut(s) 94, 198
CviQI GTAC 1 cut(s) 381
DdeI CTNAG 1 cut(s) 327
DpnI GATC 1 cut(s) 131
DpnII GATC 1 cut(s) 129
EciI GGCGGA 1 cut(s) 382
Eco24I GRGCYC 1 cut(s) 252
EcoO109I RGGNCCY 1 cut(s) 248
EcoRII CCWGG 1 cut(s) 100
EcoT38I GRGCYC 1 cut(s) 252
FaeI CATG 2 cut(s) 97, 201
FaiI YATR 4 cut(s) 37, 95, 199, 245
FatI CATG 2 cut(s) 93, 197
FauI CCCGC 1 cut(s) 451
FblI GTMKAC 1 cut(s) 157
Fnu4HI GCNGC 8 cut(s) 72, 98, 123, 363, 366, 417, 420, 423
FokI GGATG 2 cut(s) 98, 164
FriOI GRGCYC 1 cut(s) 252
Fsp4HI GCNGC 8 cut(s) 72, 98, 123, 363, 366, 417, 420, 423
FspBI CTAG 2 cut(s) 161, 378
GlaI GCGC 1 cut(s) 180
GluI GCNGC 8 cut(s) 72, 98, 123, 363, 366, 417, 420, 423
HaeIII GGCC 2 cut(s) 250, 295
HapII CCGG 1 cut(s) 26
HhaI GCGC 1 cut(s) 181
Hin1II CATG 2 cut(s) 97, 201
Hin6I GCGC 1 cut(s) 179
HinP1I GCGC 1 cut(s) 179
HpaII CCGG 1 cut(s) 26
HphI GGTGA 1 cut(s) 396
Hpy166II GTNNAC 1 cut(s) 158
Hpy188III TCNNGA 1 cut(s) 226
Hpy8I GTNNAC 1 cut(s) 158
HpyAV CCTTC 2 cut(s) 140, 408
HpyCH4III ACNGT 2 cut(s) 257, 356
HpyCH4IV ACGT 2 cut(s) 15, 81
HpyCH4V TGCA 6 cut(s) 56, 71, 97, 261, 299, 368
HpyF10VI GCNNNNNNNGC 6 cut(s) 62, 71, 187, 301, 371, 422
HpyF3I CTNAG 1 cut(s) 327
HpySE526I ACGT 2 cut(s) 15, 81
Hsp92II CATG 2 cut(s) 97, 201
HspAI GCGC 1 cut(s) 179
Kzo9I GATC 1 cut(s) 129
LmnI GCTCC 2 cut(s) 187, 206
Lsp1109I GCAGC 4 cut(s) 83, 109, 352, 403
LweI GCATC 2 cut(s) 349, 377
MaeI CTAG 2 cut(s) 161, 378
MaeII ACGT 2 cut(s) 15, 81
MaeIII GTNAC 1 cut(s) 335
MalI GATC 1 cut(s) 131
MboI GATC 1 cut(s) 129
MboII GAAGA 1 cut(s) 393
MhlI GDGCHC 1 cut(s) 252
MluCI AATT 2 cut(s) 166, 385
MnlI CCTC 7 cut(s) 202, 322, 325, 341, 400, 420, 450
MseI TTAA 1 cut(s) 87
MspA1I CMGCKG 1 cut(s) 365
MspI CCGG 1 cut(s) 26
MspR9I CCNGG 2 cut(s) 26, 102
Mva1269I GAATGC 1 cut(s) 181
MvaI CCWGG 1 cut(s) 102
MvnI CGCG 1 cut(s) 122
MwoI GCNNNNNNNGC 6 cut(s) 62, 71, 187, 301, 371, 422
NciI CCSGG 1 cut(s) 26
NdeII GATC 1 cut(s) 129
NlaIII CATG 2 cut(s) 97, 201
NlaIV GGNNCC 1 cut(s) 250
PctI GAATGC 1 cut(s) 181
PkrI GCNGC 8 cut(s) 73, 99, 124, 364, 367, 418, 421, 424
PshBI ATTAAT 1 cut(s) 87
Psp6I CCWGG 1 cut(s) 100
PspGI CCWGG 1 cut(s) 100
PspN4I GGNNCC 1 cut(s) 250
PspOMI GGGCCC 1 cut(s) 248
PspPI GGNCC 2 cut(s) 248, 249
RsaI GTAC 1 cut(s) 382
RsaNI GTAC 1 cut(s) 381
SaqAI TTAA 1 cut(s) 87
SatI GCNGC 8 cut(s) 72, 98, 123, 363, 366, 417, 420, 423
Sau3AI GATC 1 cut(s) 129
Sau96I GGNCC 2 cut(s) 248, 249
ScaI AGTACT 1 cut(s) 382
ScrFI CCNGG 2 cut(s) 26, 102
SduI GDGCHC 1 cut(s) 252
SetI ASST 5 cut(s) 18, 84, 192, 213, 412
SfaNI GCATC 2 cut(s) 349, 377
SpeI ACTAGT 1 cut(s) 377
Sse9I AATT 2 cut(s) 166, 385
SsiI CCGC 6 cut(s) 122, 363, 393, 420, 423, 444
SspI AATATT 1 cut(s) 45
SspMI CTAG 2 cut(s) 161, 378
StyD4I CCNGG 2 cut(s) 24, 100
TaaI ACNGT 2 cut(s) 257, 356
TaiI ACGT 2 cut(s) 18, 84
TaqI TCGA 1 cut(s) 311
TasI AATT 2 cut(s) 166, 385
TatI WGTACW 1 cut(s) 380
TauI GCSGC 4 cut(s) 125, 365, 422, 425
Tru1I TTAA 1 cut(s) 87
Tru9I TTAA 1 cut(s) 87
TscAI CASTG 1 cut(s) 293
TseI GCWGC 4 cut(s) 71, 97, 365, 416
TspRI CASTG 1 cut(s) 293
VspI ATTAAT 1 cut(s) 87
XmiI GTMKAC 1 cut(s) 157
XspI CTAG 2 cut(s) 161, 378
ZrmI AGTACT 1 cut(s) 382
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.