Rroxscaffold_2G00124190

Egg cell-secreted protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0008373
Physical Location & Seq
Forward (+)
58289224 .. 58290824
1601 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00124190.1

Sequence Viewer

Length: 519 bp
ATGGCTTTGAAGAATTTGGTTTTTCTTTCCATGGTGACACTGATCGCATCCCTCATAATGAGTGCAAGTAATGCAACTGCTACTAGGAAAATTTCTGCAGGGATCAAAACGAGCGGTGAAGGCGAAGGCGGGCTAGTGGAATGCGGAAATGCGCTGGTGGAGCTGAAATCTTGCTCGCAAGAGATTGTTGTGTATTTCCTGAATGGGAAGGCTAATATCGGCCCAGACTGTTGCAAAGCTATCGCCACCATTACACGTCACTGCTGGCCTGCAATGCTTACTTCCCTTGGCTTCACTGCAGAACAAGCGAGACTCCATGTAAGTAGAGTCATTCATCTGGGCATACACTCCACATTTGAAAGCATGAGACGTTCCTCCATGACCATTTGCCTCGATTTTGAGACAGCCGTCAATTTAGAGTCTGACTTTCGCAGCATCAACGTCGTGAACCACATTGATCTTGAACCATTTATTGTAGATGTTTGGAACCAGTACTGGAGCCCTGTAGTAGGTGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Prolamin_like

Protein Analysis

172

Amino Acids

18.78

Weight (kDa)

5.94

Isoelectric Point (pI)

41.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Prolamin_like PF05617 47 - 100 1.7e-15 Prolamin-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 114
AciI CCGC 3 cut(s) 114, 129, 144
AclWI GGATC 1 cut(s) 110
AcsI RAATTY 2 cut(s) 13, 90
AfaI GTAC 1 cut(s) 494
AfiI CCNNNNNNNGG 1 cut(s) 509
AflIII ACRYGT 1 cut(s) 254
AgsI TTSAA 3 cut(s) 10, 359, 464
AjiI CACGTC 1 cut(s) 257
AluBI AGCT 2 cut(s) 163, 239
AluI AGCT 2 cut(s) 163, 239
Alw26I GTCTC 3 cut(s) 304, 361, 395
AlwI GGATC 1 cut(s) 110
AoxI GGCC 2 cut(s) 220, 266
ApeKI GCWGC 1 cut(s) 432
ApoI RAATTY 2 cut(s) 13, 90
AspLEI GCGC 1 cut(s) 154
AspS9I GGNCC 1 cut(s) 221
AsuHPI GGTGA 2 cut(s) 46, 128
BanII GRGCYC 1 cut(s) 503
BbvI GCAGC 1 cut(s) 444
BceAI ACGGC 1 cut(s) 392
BcgI CGANNNNNNTGC 4 cut(s) 223, 257, 424, 458
BcoDI GTCTC 3 cut(s) 304, 361, 395
BfaI CTAG 2 cut(s) 84, 134
BfmI CTRYAG 3 cut(s) 96, 297, 504
BisI GCNGC 1 cut(s) 433
BlsI GCNGC 1 cut(s) 434
BmcAI AGTACT 1 cut(s) 494
BmgBI CACGTC 1 cut(s) 257
BmgT120I GGNCC 1 cut(s) 221
BmiI GGNNCC 2 cut(s) 488, 500
BmsI GCATC 2 cut(s) 56, 444
BoxI GACNNNNGTC 1 cut(s) 407
BpmI CTGGAG 1 cut(s) 517
BsaJI CCNNGG 2 cut(s) 30, 286
Bsc4I CCNNNNNNNGG 1 cut(s) 509
Bse1I ACTGG 2 cut(s) 490, 500
Bse3DI GCAATG 1 cut(s) 279
BseDI CCNNGG 2 cut(s) 30, 286
BseGI GGATG 1 cut(s) 47
BseLI CCNNNNNNNGG 1 cut(s) 509
BseMI GCAATG 1 cut(s) 279
BseNI ACTGG 2 cut(s) 490, 500
BseXI GCAGC 1 cut(s) 444
BshFI GGCC 2 cut(s) 222, 268
BslI CCNNNNNNNGG 1 cut(s) 509
BsmAI GTCTC 3 cut(s) 304, 361, 395
BsmBI CGTCTC 1 cut(s) 361
BsmI GAATGC 1 cut(s) 146
BsnI GGCC 2 cut(s) 222, 268
Bsp1286I GDGCHC 1 cut(s) 503
Bsp143I GATC 3 cut(s) 42, 102, 457
Bsp19I CCATGG 1 cut(s) 30
BspACI CCGC 3 cut(s) 114, 129, 144
BspANI GGCC 2 cut(s) 222, 268
BspLI GGNNCC 2 cut(s) 488, 500
BspMAI CTGCAG 2 cut(s) 100, 301
BspPI GGATC 1 cut(s) 110
BsrBI CCGCTC 1 cut(s) 114
BsrDI GCAATG 1 cut(s) 279
BsrI ACTGG 2 cut(s) 490, 500
BssECI CCNNGG 2 cut(s) 30, 286
BssMI GATC 3 cut(s) 42, 102, 457
BssT1I CCWWGG 2 cut(s) 30, 286
Bst4CI ACNGT 1 cut(s) 230
BstAPI GCANNNNNTGC 1 cut(s) 71
BstC8I GCNNGC 4 cut(s) 131, 176, 266, 270
BstDSI CCRYGG 1 cut(s) 30
BstENI CCTNNNNNAGG 1 cut(s) 507
BstF5I GGATG 1 cut(s) 47
BstHHI GCGC 1 cut(s) 154
BstKTI GATC 3 cut(s) 45, 105, 460
BstMAI GTCTC 3 cut(s) 304, 361, 395
BstMBI GATC 3 cut(s) 42, 102, 457
BstMWI GCNNNNNNNGC 5 cut(s) 71, 120, 160, 274, 305
BstPAI GACNNNNGTC 1 cut(s) 407
BstSFI CTRYAG 3 cut(s) 96, 297, 504
BstV1I GCAGC 1 cut(s) 444
BsuRI GGCC 2 cut(s) 222, 268
BtgI CCRYGG 1 cut(s) 30
BtrI CACGTC 1 cut(s) 257
BtsCI GGATG 1 cut(s) 47
BtsI GCAGTG 2 cut(s) 259, 294
BtsIMutI CAGTG 3 cut(s) 38, 259, 294
Cac8I GCNNGC 4 cut(s) 131, 176, 266, 270
CfoI GCGC 1 cut(s) 154
Cfr13I GGNCC 1 cut(s) 221
Csp6I GTAC 1 cut(s) 493
CviAII CATG 4 cut(s) 31, 317, 364, 379
CviQI GTAC 1 cut(s) 493
DpnI GATC 3 cut(s) 44, 104, 459
DpnII GATC 3 cut(s) 42, 102, 457
Eco130I CCWWGG 2 cut(s) 30, 286
Eco24I GRGCYC 1 cut(s) 503
EcoNI CCTNNNNNAGG 1 cut(s) 507
EcoT14I CCWWGG 2 cut(s) 30, 286
EcoT38I GRGCYC 1 cut(s) 503
ErhI CCWWGG 2 cut(s) 30, 286
Esp3I CGTCTC 1 cut(s) 361
FaeI CATG 4 cut(s) 34, 320, 367, 382
FaiI YATR 6 cut(s) 32, 56, 318, 344, 365, 380
FatI CATG 4 cut(s) 30, 316, 363, 378
FauI CCCGC 1 cut(s) 122
Fnu4HI GCNGC 1 cut(s) 433
FokI GGATG 1 cut(s) 34
FriOI GRGCYC 1 cut(s) 503
Fsp4HI GCNGC 1 cut(s) 433
FspBI CTAG 2 cut(s) 84, 134
GlaI GCGC 1 cut(s) 153
GluI GCNGC 1 cut(s) 433
GsuI CTGGAG 1 cut(s) 517
HaeIII GGCC 2 cut(s) 222, 268
HhaI GCGC 1 cut(s) 154
Hin1II CATG 4 cut(s) 34, 320, 367, 382
Hin6I GCGC 1 cut(s) 152
HinP1I GCGC 1 cut(s) 152
HinfI GANTC 3 cut(s) 312, 327, 419
HphI GGTGA 2 cut(s) 46, 128
Hpy166II GTNNAC 1 cut(s) 448
Hpy188I TCNGA 1 cut(s) 424
Hpy188III TCNNGA 3 cut(s) 199, 445, 461
Hpy8I GTNNAC 1 cut(s) 448
Hpy99I CGWCG 1 cut(s) 446
HpyAV CCTTC 3 cut(s) 113, 119, 202
HpyCH4III ACNGT 1 cut(s) 230
HpyCH4IV ACGT 3 cut(s) 256, 370, 441
HpyCH4V TGCA 6 cut(s) 65, 74, 98, 234, 272, 299
HpyF10VI GCNNNNNNNGC 5 cut(s) 71, 120, 160, 274, 305
HpySE526I ACGT 3 cut(s) 256, 370, 441
Hsp92II CATG 4 cut(s) 34, 320, 367, 382
HspAI GCGC 1 cut(s) 152
Kzo9I GATC 3 cut(s) 42, 102, 457
LmnI GCTCC 2 cut(s) 160, 498
LpnPI CCDG 9 cut(s) 84, 140, 212, 237, 250, 282, 323, 481, 503
Lsp1109I GCAGC 1 cut(s) 444
LweI GCATC 2 cut(s) 56, 444
MaeI CTAG 2 cut(s) 84, 134
MaeII ACGT 3 cut(s) 256, 370, 441
MaeIII GTNAC 2 cut(s) 34, 257
MalI GATC 3 cut(s) 44, 104, 459
MbiI CCGCTC 1 cut(s) 114
MboI GATC 3 cut(s) 42, 102, 457
MboII GAAGA 1 cut(s) 22
MhlI GDGCHC 1 cut(s) 503
MluCI AATT 3 cut(s) 13, 90, 412
MlyI GAGTC 3 cut(s) 306, 336, 428
MnlI CCTC 3 cut(s) 62, 385, 401
Mva1269I GAATGC 1 cut(s) 146
MwoI GCNNNNNNNGC 5 cut(s) 71, 120, 160, 274, 305
NcoI CCATGG 1 cut(s) 30
NdeII GATC 3 cut(s) 42, 102, 457
NlaIII CATG 4 cut(s) 34, 320, 367, 382
NlaIV GGNNCC 2 cut(s) 488, 500
NmuCI GTSAC 2 cut(s) 34, 257
PctI GAATGC 1 cut(s) 146
PkrI GCNGC 1 cut(s) 434
PleI GAGTC 3 cut(s) 306, 335, 427
PpsI GAGTC 3 cut(s) 306, 335, 427
PshAI GACNNNNGTC 1 cut(s) 407
PspN4I GGNNCC 2 cut(s) 488, 500
PspPI GGNCC 1 cut(s) 221
PstI CTGCAG 2 cut(s) 100, 301
RsaI GTAC 1 cut(s) 494
RsaNI GTAC 1 cut(s) 493
SatI GCNGC 1 cut(s) 433
Sau3AI GATC 3 cut(s) 42, 102, 457
Sau96I GGNCC 1 cut(s) 221
ScaI AGTACT 1 cut(s) 494
SchI GAGTC 3 cut(s) 306, 336, 428
SduI GDGCHC 1 cut(s) 503
SetI ASST 6 cut(s) 165, 241, 259, 373, 444, 514
SfaNI GCATC 2 cut(s) 56, 444
SfcI CTRYAG 3 cut(s) 96, 297, 504
Sse9I AATT 3 cut(s) 13, 90, 412
SsiI CCGC 3 cut(s) 114, 129, 144
SspMI CTAG 2 cut(s) 84, 134
StyI CCWWGG 2 cut(s) 30, 286
TaaI ACNGT 1 cut(s) 230
TaiI ACGT 3 cut(s) 259, 373, 444
TaqI TCGA 1 cut(s) 393
TasI AATT 3 cut(s) 13, 90, 412
TatI WGTACW 1 cut(s) 492
TscAI CASTG 3 cut(s) 45, 266, 301
TseFI GTSAC 2 cut(s) 34, 257
TseI GCWGC 1 cut(s) 432
Tsp45I GTSAC 2 cut(s) 34, 257
TspDTI ATGAA 1 cut(s) 323
TspRI CASTG 3 cut(s) 45, 266, 301
XagI CCTNNNNNAGG 1 cut(s) 507
XapI RAATTY 2 cut(s) 13, 90
XspI CTAG 2 cut(s) 84, 134
ZrmI AGTACT 1 cut(s) 494
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.