MD15G1340900.v1.1

Egg cell-secreted protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0008373
Physical Location & Seq
Forward (+)
38602975 .. 38603424
450 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1340900.v1.1.491

Sequence Viewer

Length: 450 bp
ATGGCTTTACAAAATGTAGCTCTCCTGGTGACAGTATGCCTAATATTCACCGGTGCAAACTATGCACTTGCAGCAAGGAACGTGATTGATCCCATTCAGCCAGCCTACAGCATCCCCATCACAGCGAGAATCAAAACTGGAAGTGAAGGAAATGGCGGGCTAGTCGATTGCTGGAATGCGCTTGCGGAGCTGAAATCATGCTCCAATGAGGTGGTTCTGTTTTTCCTGAATGGGCAGGCTGATATAGGGCCTGATTGTTGCCAAGCCATTGCCACTATTACGCGCCACTGCTGGCCTGCAATGCTGACTTCCATCGGTTTCACGACTGAGGAGGGTAACATTTTGCTAGGCTACTGTGATGCCGCTGCATCTGCTACTACTACTAATTCCTCCGCCCCTGCTTCATCTCCTCTTGCTGCCGCCACCACGCCTACTCCTCCCGCCAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Prolamin_like

Protein Analysis

150

Amino Acids

15.35

Weight (kDa)

4.45

Isoelectric Point (pI)

38.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Prolamin_like PF05617 56 - 120 3.5e-20 Prolamin-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 283
AciI CCGC 6 cut(s) 156, 185, 363, 393, 420, 441
AclWI GGATC 1 cut(s) 83
AgeI ACCGGT 1 cut(s) 50
AjnI CCWGG 1 cut(s) 24
AluBI AGCT 2 cut(s) 20, 190
AluI AGCT 2 cut(s) 20, 190
AlwI GGATC 1 cut(s) 83
AoxI GGCC 2 cut(s) 248, 293
ApeKI GCWGC 3 cut(s) 71, 365, 416
AsiGI ACCGGT 1 cut(s) 50
AspLEI GCGC 2 cut(s) 181, 285
AspS9I GGNCC 1 cut(s) 248
AsuHPI GGTGA 2 cut(s) 40, 40
BbvI GCAGC 3 cut(s) 83, 352, 403
BccI CCATC 2 cut(s) 125, 320
BciT130I CCWGG 1 cut(s) 26
BfaI CTAG 2 cut(s) 161, 347
BfmI CTRYAG 1 cut(s) 106
BisI GCNGC 5 cut(s) 72, 363, 366, 417, 420
BlsI GCNGC 5 cut(s) 73, 364, 367, 418, 421
Bme1390I CCNGG 1 cut(s) 26
BmgT120I GGNCC 1 cut(s) 248
BmrFI CCNGG 1 cut(s) 26
BmsI GCATC 3 cut(s) 120, 349, 377
BsaWI WCCGGW 1 cut(s) 50
BsaXI ACNNNNNCTCC 2 cut(s) 418, 448
Bse118I RCCGGY 1 cut(s) 50
Bse1I ACTGG 1 cut(s) 142
Bse3DI GCAATG 2 cut(s) 267, 306
BseBI CCWGG 1 cut(s) 26
BseGI GGATG 1 cut(s) 111
BseMI GCAATG 2 cut(s) 267, 306
BseMII CTCAG 1 cut(s) 318
BseNI ACTGG 1 cut(s) 142
BseRI GAGGAG 3 cut(s) 344, 399, 426
BseXI GCAGC 3 cut(s) 83, 352, 403
Bsh1236I CGCG 1 cut(s) 283
BshFI GGCC 2 cut(s) 250, 295
BshTI ACCGGT 1 cut(s) 50
BsiSI CCGG 1 cut(s) 51
BsmI GAATGC 1 cut(s) 181
BsnI GGCC 2 cut(s) 250, 295
Bsp143I GATC 1 cut(s) 88
BspACI CCGC 6 cut(s) 156, 185, 363, 393, 420, 441
BspANI GGCC 2 cut(s) 250, 295
BspCNI CTCAG 1 cut(s) 319
BspFNI CGCG 1 cut(s) 283
BspPI GGATC 1 cut(s) 83
BsrDI GCAATG 2 cut(s) 267, 306
BsrFI RCCGGY 1 cut(s) 50
BsrI ACTGG 1 cut(s) 142
BssAI RCCGGY 1 cut(s) 50
BssMI GATC 1 cut(s) 88
Bst2UI CCWGG 1 cut(s) 26
Bst4CI ACNGT 2 cut(s) 34, 356
BstAPI GCANNNNNTGC 1 cut(s) 62
BstC8I GCNNGC 6 cut(s) 102, 158, 183, 237, 293, 297
BstDEI CTNAG 1 cut(s) 327
BstF5I GGATG 1 cut(s) 111
BstFNI CGCG 1 cut(s) 283
BstHHI GCGC 2 cut(s) 181, 285
BstKTI GATC 1 cut(s) 91
BstMBI GATC 1 cut(s) 88
BstMWI GCNNNNNNNGC 5 cut(s) 62, 71, 187, 301, 371
BstNI CCWGG 1 cut(s) 26
BstSCI CCNGG 1 cut(s) 24
BstSFI CTRYAG 1 cut(s) 106
BstUI CGCG 1 cut(s) 283
BstV1I GCAGC 3 cut(s) 83, 352, 403
BstXI CCANNNNNNTGG 1 cut(s) 211
BsuRI GGCC 2 cut(s) 250, 295
BtsCI GGATG 1 cut(s) 111
BtsI GCAGTG 1 cut(s) 286
BtsIMutI CAGTG 1 cut(s) 286
Cac8I GCNNGC 6 cut(s) 102, 158, 183, 237, 293, 297
CfoI GCGC 2 cut(s) 181, 285
Cfr10I RCCGGY 1 cut(s) 50
Cfr13I GGNCC 1 cut(s) 248
CspAI ACCGGT 1 cut(s) 50
CviAII CATG 1 cut(s) 198
DdeI CTNAG 1 cut(s) 327
DpnI GATC 1 cut(s) 90
DpnII GATC 1 cut(s) 88
EciI GGCGGA 1 cut(s) 382
EcoO109I RGGNCCY 1 cut(s) 248
EcoRII CCWGG 1 cut(s) 24
FaeI CATG 1 cut(s) 201
FaiI YATR 4 cut(s) 37, 63, 199, 245
FatI CATG 1 cut(s) 197
FauI CCCGC 2 cut(s) 149, 448
Fnu4HI GCNGC 5 cut(s) 72, 363, 366, 417, 420
FokI GGATG 1 cut(s) 98
Fsp4HI GCNGC 5 cut(s) 72, 363, 366, 417, 420
FspBI CTAG 2 cut(s) 161, 347
GlaI GCGC 2 cut(s) 180, 284
GluI GCNGC 5 cut(s) 72, 363, 366, 417, 420
HaeIII GGCC 2 cut(s) 250, 295
HapII CCGG 1 cut(s) 51
HhaI GCGC 2 cut(s) 181, 285
Hin1II CATG 1 cut(s) 201
Hin6I GCGC 2 cut(s) 179, 283
HinP1I GCGC 2 cut(s) 179, 283
HinfI GANTC 1 cut(s) 129
HpaII CCGG 1 cut(s) 51
HphI GGTGA 2 cut(s) 40, 40
Hpy188III TCNNGA 2 cut(s) 226, 322
HpyAV CCTTC 1 cut(s) 140
HpyCH4III ACNGT 2 cut(s) 34, 356
HpyCH4IV ACGT 1 cut(s) 81
HpyCH4V TGCA 5 cut(s) 56, 65, 71, 299, 368
HpyF10VI GCNNNNNNNGC 5 cut(s) 62, 71, 187, 301, 371
HpyF3I CTNAG 1 cut(s) 327
HpySE526I ACGT 1 cut(s) 81
Hsp92II CATG 1 cut(s) 201
HspAI GCGC 2 cut(s) 179, 283
Kzo9I GATC 1 cut(s) 88
LmnI GCTCC 2 cut(s) 187, 206
Lsp1109I GCAGC 3 cut(s) 83, 352, 403
LweI GCATC 3 cut(s) 120, 349, 377
MaeI CTAG 2 cut(s) 161, 347
MaeII ACGT 1 cut(s) 81
MaeIII GTNAC 2 cut(s) 28, 335
MalI GATC 1 cut(s) 90
MboI GATC 1 cut(s) 88
MluCI AATT 1 cut(s) 385
MnlI CCTC 6 cut(s) 202, 322, 325, 400, 420, 447
MspA1I CMGCKG 1 cut(s) 365
MspI CCGG 1 cut(s) 51
MspR9I CCNGG 1 cut(s) 26
Mva1269I GAATGC 1 cut(s) 181
MvaI CCWGG 1 cut(s) 26
MvnI CGCG 1 cut(s) 283
MwoI GCNNNNNNNGC 5 cut(s) 62, 71, 187, 301, 371
NdeII GATC 1 cut(s) 88
NlaIII CATG 1 cut(s) 201
NmuCI GTSAC 1 cut(s) 28
PctI GAATGC 1 cut(s) 181
PfeI GAWTC 1 cut(s) 129
PinAI ACCGGT 1 cut(s) 50
PkrI GCNGC 5 cut(s) 73, 364, 367, 418, 421
Psp6I CCWGG 1 cut(s) 24
PspGI CCWGG 1 cut(s) 24
PspPI GGNCC 1 cut(s) 248
SatI GCNGC 5 cut(s) 72, 363, 366, 417, 420
Sau3AI GATC 1 cut(s) 88
Sau96I GGNCC 1 cut(s) 248
ScrFI CCNGG 1 cut(s) 26
SetI ASST 4 cut(s) 22, 84, 192, 213
SfaNI GCATC 3 cut(s) 120, 349, 377
SfcI CTRYAG 1 cut(s) 106
SgrAI CRCCGGYG 1 cut(s) 50
Sse9I AATT 1 cut(s) 385
SsiI CCGC 6 cut(s) 156, 185, 363, 393, 420, 441
SspI AATATT 1 cut(s) 45
SspMI CTAG 2 cut(s) 161, 347
StyD4I CCNGG 1 cut(s) 24
TaaI ACNGT 2 cut(s) 34, 356
TaiI ACGT 1 cut(s) 84
TaqI TCGA 1 cut(s) 165
TasI AATT 1 cut(s) 385
TauI GCSGC 2 cut(s) 365, 422
TfiI GAWTC 1 cut(s) 129
TscAI CASTG 1 cut(s) 293
TseFI GTSAC 1 cut(s) 28
TseI GCWGC 3 cut(s) 71, 365, 416
Tsp45I GTSAC 1 cut(s) 28
TspDTI ATGAA 1 cut(s) 393
TspRI CASTG 1 cut(s) 293
XspI CTAG 2 cut(s) 161, 347
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.