RLG00000018490

Egg cell-secreted protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0008373
Physical Location & Seq
Reverse (-)
30677508 .. 30677909
402 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000018490

Sequence Viewer

Length: 402 bp
ATGGCTTTGAAGAATTTGGTTTTGGTTTCCATGGTGACACTGATCGCATCCCTCATACTGAGTGCAAGTAATGCAACTGCTACTAGGAAAATTTATGACAAAACGAGCGGTGAAGGCGAAGGCTGGCTAGTGGAATGCGGAAATGCACTGGTGGAGCTGAAATCTTGCTCGCAAGAGATTGTTCTGTATTTCCTGAATGGGAAGGCTAATATCGGTCCACACTGTTGCAAAGCTATCGCCACCATTACACGTCACTGCTGGCCTGCAATGCTTACTTCCCTTGGCTTCACTGCAGAACAAGGTGACGTCTTACGAGGCTACTGTGATGCTGCGGCTGCTGTGACTACTACTGCTCCTGCGGCAGCGCCACTTGCTCGTACAACACCTCTTACCAGTTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Prolamin_like

Protein Analysis

134

Amino Acids

13.92

Weight (kDa)

7.56

Isoelectric Point (pI)

35.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Prolamin_like PF05617 45 - 109 1.1e-18 Prolamin-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 309
AccBSI CCGCTC 1 cut(s) 108
AciI CCGC 4 cut(s) 108, 138, 332, 359
AcsI RAATTY 2 cut(s) 13, 90
AcyI GRCGYC 1 cut(s) 306
AfaI GTAC 1 cut(s) 379
AflIII ACRYGT 1 cut(s) 248
AgsI TTSAA 1 cut(s) 10
AjiI CACGTC 1 cut(s) 251
AjuI GAANNNNNNNTTGG 1 cut(s) 37
AluBI AGCT 2 cut(s) 157, 233
AluI AGCT 2 cut(s) 157, 233
AoxI GGCC 1 cut(s) 260
ApeKI GCWGC 3 cut(s) 329, 335, 362
ApoI RAATTY 2 cut(s) 13, 90
AspLEI GCGC 1 cut(s) 367
AspS9I GGNCC 1 cut(s) 215
AsuHPI GGTGA 3 cut(s) 46, 122, 314
AvaII GGWCC 1 cut(s) 215
BbvI GCAGC 3 cut(s) 316, 322, 374
BcgI CGANNNNNNTGC 2 cut(s) 217, 251
BfaI CTAG 2 cut(s) 84, 128
BfmI CTRYAG 1 cut(s) 291
BfoI RGCGCY 1 cut(s) 368
BisI GCNGC 5 cut(s) 330, 333, 336, 360, 363
BlsI GCNGC 5 cut(s) 331, 334, 337, 361, 364
Bme18I GGWCC 1 cut(s) 215
BmgBI CACGTC 1 cut(s) 251
BmgT120I GGNCC 1 cut(s) 215
BmsI GCATC 2 cut(s) 56, 316
BsaHI GRCGYC 1 cut(s) 306
BsaJI CCNNGG 2 cut(s) 30, 280
BsaXI ACNNNNNCTCC 2 cut(s) 337, 367
Bse1I ACTGG 2 cut(s) 153, 393
Bse3DI GCAATG 1 cut(s) 273
BseDI CCNNGG 2 cut(s) 30, 280
BseGI GGATG 1 cut(s) 47
BseMI GCAATG 1 cut(s) 273
BseMII CTCAG 1 cut(s) 50
BseNI ACTGG 2 cut(s) 153, 393
BseXI GCAGC 3 cut(s) 316, 322, 374
BshFI GGCC 1 cut(s) 262
BsmI GAATGC 1 cut(s) 140
BsnI GGCC 1 cut(s) 262
Bsp143I GATC 1 cut(s) 42
Bsp19I CCATGG 1 cut(s) 30
BspACI CCGC 4 cut(s) 108, 138, 332, 359
BspANI GGCC 1 cut(s) 262
BspCNI CTCAG 1 cut(s) 51
BspMAI CTGCAG 1 cut(s) 295
BsrBI CCGCTC 1 cut(s) 108
BsrDI GCAATG 1 cut(s) 273
BsrI ACTGG 2 cut(s) 153, 393
BssECI CCNNGG 2 cut(s) 30, 280
BssMI GATC 1 cut(s) 42
BssNI GRCGYC 1 cut(s) 306
BssT1I CCWWGG 2 cut(s) 30, 280
Bst4CI ACNGT 2 cut(s) 224, 323
BstACI GRCGYC 1 cut(s) 306
BstAPI GCANNNNNTGC 1 cut(s) 71
BstC8I GCNNGC 4 cut(s) 125, 170, 260, 264
BstDEI CTNAG 1 cut(s) 59
BstDSI CCRYGG 1 cut(s) 30
BstF5I GGATG 1 cut(s) 47
BstH2I RGCGCY 1 cut(s) 368
BstHHI GCGC 1 cut(s) 367
BstKTI GATC 1 cut(s) 45
BstMBI GATC 1 cut(s) 42
BstMWI GCNNNNNNNGC 6 cut(s) 71, 114, 268, 335, 359, 371
BstSFI CTRYAG 1 cut(s) 291
BstV1I GCAGC 3 cut(s) 316, 322, 374
BsuRI GGCC 1 cut(s) 262
BtgI CCRYGG 1 cut(s) 30
BtrI CACGTC 1 cut(s) 251
BtsCI GGATG 1 cut(s) 47
BtsI GCAGTG 2 cut(s) 253, 288
BtsIMutI CAGTG 5 cut(s) 38, 146, 220, 253, 288
Cac8I GCNNGC 4 cut(s) 125, 170, 260, 264
CfoI GCGC 1 cut(s) 367
Cfr13I GGNCC 1 cut(s) 215
Csp6I GTAC 1 cut(s) 378
CviAII CATG 1 cut(s) 31
CviQI GTAC 1 cut(s) 378
DdeI CTNAG 1 cut(s) 59
DpnI GATC 1 cut(s) 44
DpnII GATC 1 cut(s) 42
Eco130I CCWWGG 2 cut(s) 30, 280
Eco47I GGWCC 1 cut(s) 215
EcoT14I CCWWGG 2 cut(s) 30, 280
ErhI CCWWGG 2 cut(s) 30, 280
FaeI CATG 1 cut(s) 34
FaiI YATR 3 cut(s) 32, 56, 96
FatI CATG 1 cut(s) 30
Fnu4HI GCNGC 5 cut(s) 330, 333, 336, 360, 363
FokI GGATG 1 cut(s) 34
Fsp4HI GCNGC 5 cut(s) 330, 333, 336, 360, 363
FspBI CTAG 2 cut(s) 84, 128
GlaI GCGC 1 cut(s) 366
GluI GCNGC 5 cut(s) 330, 333, 336, 360, 363
HaeII RGCGCY 1 cut(s) 368
HaeIII GGCC 1 cut(s) 262
HhaI GCGC 1 cut(s) 367
Hin1I GRCGYC 1 cut(s) 306
Hin1II CATG 1 cut(s) 34
Hin6I GCGC 1 cut(s) 365
HinP1I GCGC 1 cut(s) 365
HphI GGTGA 3 cut(s) 46, 122, 314
Hpy166II GTNNAC 1 cut(s) 218
Hpy188III TCNNGA 1 cut(s) 193
Hpy8I GTNNAC 1 cut(s) 218
HpyAV CCTTC 3 cut(s) 107, 113, 196
HpyCH4III ACNGT 2 cut(s) 224, 323
HpyCH4IV ACGT 2 cut(s) 250, 306
HpyCH4V TGCA 6 cut(s) 65, 74, 146, 228, 266, 293
HpyF10VI GCNNNNNNNGC 6 cut(s) 71, 114, 268, 335, 359, 371
HpyF3I CTNAG 1 cut(s) 59
HpySE526I ACGT 2 cut(s) 250, 306
Hsp92I GRCGYC 1 cut(s) 306
Hsp92II CATG 1 cut(s) 34
HspAI GCGC 1 cut(s) 365
Kzo9I GATC 1 cut(s) 42
LmnI GCTCC 2 cut(s) 154, 358
LpnPI CCDG 6 cut(s) 109, 134, 206, 244, 276, 369
Lsp1109I GCAGC 3 cut(s) 316, 322, 374
LweI GCATC 2 cut(s) 56, 316
MaeI CTAG 2 cut(s) 84, 128
MaeII ACGT 2 cut(s) 250, 306
MaeIII GTNAC 4 cut(s) 34, 251, 302, 340
MalI GATC 1 cut(s) 44
MbiI CCGCTC 1 cut(s) 108
MboI GATC 1 cut(s) 42
MboII GAAGA 1 cut(s) 22
MluCI AATT 2 cut(s) 13, 90
MnlI CCTC 3 cut(s) 62, 308, 396
Mva1269I GAATGC 1 cut(s) 140
MwoI GCNNNNNNNGC 6 cut(s) 71, 114, 268, 335, 359, 371
NcoI CCATGG 1 cut(s) 30
NdeII GATC 1 cut(s) 42
NlaIII CATG 1 cut(s) 34
NmuCI GTSAC 4 cut(s) 34, 251, 302, 340
PctI GAATGC 1 cut(s) 140
PkrI GCNGC 5 cut(s) 331, 334, 337, 361, 364
PspPI GGNCC 1 cut(s) 215
PstI CTGCAG 1 cut(s) 295
RsaI GTAC 1 cut(s) 379
RsaNI GTAC 1 cut(s) 378
SatI GCNGC 5 cut(s) 330, 333, 336, 360, 363
Sau3AI GATC 1 cut(s) 42
Sau96I GGNCC 1 cut(s) 215
SetI ASST 6 cut(s) 159, 235, 253, 304, 309, 388
SfaNI GCATC 2 cut(s) 56, 316
SfcI CTRYAG 1 cut(s) 291
SinI GGWCC 1 cut(s) 215
Sse9I AATT 2 cut(s) 13, 90
SsiI CCGC 4 cut(s) 108, 138, 332, 359
SspMI CTAG 2 cut(s) 84, 128
StyI CCWWGG 2 cut(s) 30, 280
TaaI ACNGT 2 cut(s) 224, 323
TaiI ACGT 2 cut(s) 253, 309
TaqII GACCGA 1 cut(s) 203
TasI AATT 2 cut(s) 13, 90
TauI GCSGC 2 cut(s) 335, 362
TscAI CASTG 5 cut(s) 45, 153, 227, 260, 295
TseFI GTSAC 4 cut(s) 34, 251, 302, 340
TseI GCWGC 3 cut(s) 329, 335, 362
Tsp45I GTSAC 4 cut(s) 34, 251, 302, 340
TspRI CASTG 5 cut(s) 45, 153, 227, 260, 295
VpaK11BI GGWCC 1 cut(s) 215
XapI RAATTY 2 cut(s) 13, 90
XspI CTAG 2 cut(s) 84, 128
ZraI GACGTC 1 cut(s) 307
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.