AT2G25770

Lachrymatory-factor synthase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0006645
Physical Location & Seq
Forward (+)
10992728 .. 10993652
925 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G25770.1

Sequence Viewer

Length: 504 bp
ATGGAAAAAGCTTCAAGCCCAGAAAAATGGCTAGCCAAAGTATCAGTTACGCTCACAAAGGCAAAGCCTGACGAAATCTGGCCTCTCTTCACCGATTTTTTCAATCTCCACAAGTGGCTTCCAACGCTAGCCACATGTCACGGCGTCCACGGCAACAACGGTGAACAGGGTTGCATCAGATTCTGTTCCGGCTTTTCCATAGGATCCAACGGTGTAGACTCCGCCGCGAGATGGTCAAAGGAGAAGCTTGTGGCCGTTAATCCTGTTGAACGTGTGATGAGATACGAGATAGTGGAGAGTAACACTGGATTCGAATCCTACGTTTCGACGGTGAAGATATTGCCACGTGGCGAAGATGGTTGTGTGATTGAGTGGAGTTTCACCGTTGATCCTGTGAGAGGATTGTCGTTAGAAAATTTGGTTAAGAAGTATGAGAAAGCATTAGAGATAATCACCAAGAACATGGAAGAAGATGCTTTGAGAAGAAGAGAGTCGTCTTCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Polyketide_cyc2

Protein Analysis

167

Amino Acids

18.8

Weight (kDa)

6.33

Isoelectric Point (pI)

38.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Polyketide_cyc2 PF10604 13 - 156 4.4e-25 Polyketide cyclase / dehydrase and lipid transport
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 216
AccII CGCG 1 cut(s) 227
AciI CCGC 2 cut(s) 222, 225
AclWI GGATC 3 cut(s) 198, 211, 383
AcoI YGGCCR 1 cut(s) 252
AcsI RAATTY 1 cut(s) 415
AcvI CACGTG 1 cut(s) 347
AcyI GRCGYC 1 cut(s) 144
AfiI CCNNNNNNNGG 2 cut(s) 231, 398
AflIII ACRYGT 2 cut(s) 134, 271
AgsI TTSAA 3 cut(s) 15, 103, 269
AluBI AGCT 2 cut(s) 11, 247
AluI AGCT 2 cut(s) 11, 247
AlwI GGATC 3 cut(s) 198, 211, 383
AlwNI CAGNNNCTG 1 cut(s) 183
AoxI GGCC 2 cut(s) 80, 252
ApoI RAATTY 1 cut(s) 415
AsuHPI GGTGA 5 cut(s) 82, 173, 343, 373, 445
AsuII TTCGAA 1 cut(s) 312
AsuNHI GCTAGC 2 cut(s) 31, 127
BamHI GGATCC 1 cut(s) 203
BbrPI CACGTG 1 cut(s) 347
BbsI GAAGAC 1 cut(s) 489
BccI CCATC 2 cut(s) 225, 350
BceAI ACGGC 3 cut(s) 157, 166, 239
BfaI CTAG 2 cut(s) 32, 128
BisI GCNGC 1 cut(s) 225
BlsI GCNGC 1 cut(s) 226
BmiI GGNNCC 1 cut(s) 205
BmsI GCATC 2 cut(s) 183, 463
BmtI GCTAGC 2 cut(s) 35, 131
BpiI GAAGAC 1 cut(s) 489
Bpu14I TTCGAA 1 cut(s) 312
BsaAI YACGTR 1 cut(s) 347
BsaBI GATNNNNATC 1 cut(s) 313
BsaHI GRCGYC 1 cut(s) 144
BsaJI CCNNGG 1 cut(s) 148
Bsc4I CCNNNNNNNGG 2 cut(s) 231, 398
Bse1I ACTGG 1 cut(s) 310
Bse8I GATNNNNATC 1 cut(s) 313
BseDI CCNNGG 1 cut(s) 148
BseJI GATNNNNATC 1 cut(s) 313
BseLI CCNNNNNNNGG 2 cut(s) 231, 398
BseNI ACTGG 1 cut(s) 310
Bsh1236I CGCG 1 cut(s) 227
BshFI GGCC 2 cut(s) 82, 254
BsiSI CCGG 1 cut(s) 189
BslI CCNNNNNNNGG 2 cut(s) 231, 398
BsnI GGCC 2 cut(s) 82, 254
Bsp119I TTCGAA 1 cut(s) 312
Bsp143I GATC 2 cut(s) 203, 388
BspACI CCGC 2 cut(s) 222, 225
BspANI GGCC 2 cut(s) 82, 254
BspFNI CGCG 1 cut(s) 227
BspLI GGNNCC 1 cut(s) 205
BspOI GCTAGC 2 cut(s) 35, 131
BspPI GGATC 3 cut(s) 198, 211, 383
BspT104I TTCGAA 1 cut(s) 312
BsrI ACTGG 1 cut(s) 310
BssECI CCNNGG 1 cut(s) 148
BssMI GATC 2 cut(s) 203, 388
BssNI GRCGYC 1 cut(s) 144
Bst4CI ACNGT 4 cut(s) 161, 212, 331, 385
Bst6I CTCTTC 2 cut(s) 92, 481
BstACI GRCGYC 1 cut(s) 144
BstBAI YACGTR 1 cut(s) 347
BstBI TTCGAA 1 cut(s) 312
BstC8I GCNNGC 2 cut(s) 33, 129
BstDEI CTNAG 1 cut(s) 501
BstDSI CCRYGG 1 cut(s) 148
BstENI CCTNNNNNAGG 1 cut(s) 396
BstFNI CGCG 1 cut(s) 227
BstKTI GATC 2 cut(s) 206, 391
BstMBI GATC 2 cut(s) 203, 388
BstMWI GCNNNNNNNGC 2 cut(s) 124, 150
BstNSI RCATGY 1 cut(s) 138
BstUI CGCG 1 cut(s) 227
BstV2I GAAGAC 1 cut(s) 489
BstX2I RGATCY 1 cut(s) 203
BstXI CCANNNNNNTGG 2 cut(s) 27, 463
BstYI RGATCY 1 cut(s) 203
BsuRI GGCC 2 cut(s) 82, 254
BtgI CCRYGG 1 cut(s) 148
BtsIMutI CAGTG 1 cut(s) 303
Cac8I GCNNGC 2 cut(s) 33, 129
CaiI CAGNNNCTG 1 cut(s) 183
CseI GACGC 1 cut(s) 133
CviAII CATG 2 cut(s) 135, 463
DdeI CTNAG 1 cut(s) 501
DpnI GATC 2 cut(s) 205, 390
DpnII GATC 2 cut(s) 203, 388
EaeI YGGCCR 1 cut(s) 252
Eam1104I CTCTTC 2 cut(s) 92, 481
EarI CTCTTC 2 cut(s) 92, 481
EciI GGCGGA 1 cut(s) 211
Eco72I CACGTG 1 cut(s) 347
EcoNI CCTNNNNNAGG 1 cut(s) 396
FaeI CATG 2 cut(s) 138, 466
FaiI YATR 4 cut(s) 136, 200, 432, 464
FatI CATG 2 cut(s) 134, 462
FblI GTMKAC 1 cut(s) 216
Fnu4HI GCNGC 1 cut(s) 225
Fsp4HI GCNGC 1 cut(s) 225
FspBI CTAG 2 cut(s) 32, 128
GluI GCNGC 1 cut(s) 225
HaeIII GGCC 2 cut(s) 82, 254
HapII CCGG 1 cut(s) 189
HgaI GACGC 1 cut(s) 133
Hin1I GRCGYC 1 cut(s) 144
Hin1II CATG 2 cut(s) 138, 466
HindIII AAGCTT 2 cut(s) 9, 245
HinfI GANTC 5 cut(s) 180, 218, 309, 314, 491
HpaII CCGG 1 cut(s) 189
HphI GGTGA 5 cut(s) 82, 173, 343, 373, 445
Hpy166II GTNNAC 3 cut(s) 148, 164, 217
Hpy188I TCNGA 1 cut(s) 179
Hpy8I GTNNAC 3 cut(s) 148, 164, 217
Hpy99I CGWCG 1 cut(s) 331
HpyCH4III ACNGT 4 cut(s) 161, 212, 331, 385
HpyCH4IV ACGT 3 cut(s) 271, 321, 346
HpyCH4V TGCA 1 cut(s) 174
HpyF10VI GCNNNNNNNGC 2 cut(s) 124, 150
HpyF3I CTNAG 1 cut(s) 501
HpySE526I ACGT 3 cut(s) 271, 321, 346
Hsp92I GRCGYC 1 cut(s) 144
Hsp92II CATG 2 cut(s) 138, 466
Kzo9I GATC 2 cut(s) 203, 388
LpnPI CCDG 8 cut(s) 33, 64, 81, 152, 202, 276, 291, 405
LweI GCATC 2 cut(s) 183, 463
MaeI CTAG 2 cut(s) 32, 128
MaeII ACGT 3 cut(s) 271, 321, 346
MaeIII GTNAC 3 cut(s) 46, 137, 299
MalI GATC 2 cut(s) 205, 390
MboI GATC 2 cut(s) 203, 388
MboII GAAGA 8 cut(s) 79, 346, 365, 479, 482, 489, 495, 498
MflI RGATCY 1 cut(s) 203
MluCI AATT 1 cut(s) 415
MlyI GAGTC 2 cut(s) 212, 500
MmeI TCCRAC 2 cut(s) 146, 231
MnlI CCTC 2 cut(s) 93, 392
MseI TTAA 2 cut(s) 258, 423
MspI CCGG 1 cut(s) 189
MvnI CGCG 1 cut(s) 227
MwoI GCNNNNNNNGC 2 cut(s) 124, 150
NdeII GATC 2 cut(s) 203, 388
NheI GCTAGC 2 cut(s) 31, 127
NlaIII CATG 2 cut(s) 138, 466
NlaIV GGNNCC 1 cut(s) 205
NmuCI GTSAC 1 cut(s) 137
NspI RCATGY 1 cut(s) 138
NspV TTCGAA 1 cut(s) 312
PciI ACATGT 1 cut(s) 134
PcsI WCGNNNNNNNCGW 1 cut(s) 318
PfeI GAWTC 3 cut(s) 180, 309, 314
PkrI GCNGC 1 cut(s) 226
PleI GAGTC 2 cut(s) 212, 499
PmaCI CACGTG 1 cut(s) 347
PmlI CACGTG 1 cut(s) 347
PpsI GAGTC 2 cut(s) 212, 499
Ppu21I YACGTR 1 cut(s) 347
PscI ACATGT 1 cut(s) 134
PspCI CACGTG 1 cut(s) 347
PspN4I GGNNCC 1 cut(s) 205
PstNI CAGNNNCTG 1 cut(s) 183
PsuI RGATCY 1 cut(s) 203
SaqAI TTAA 2 cut(s) 258, 423
SatI GCNGC 1 cut(s) 225
Sau3AI GATC 2 cut(s) 203, 388
SchI GAGTC 2 cut(s) 212, 500
SetI ASST 5 cut(s) 13, 249, 274, 324, 349
SfaNI GCATC 2 cut(s) 183, 463
SfuI TTCGAA 1 cut(s) 312
Sse9I AATT 1 cut(s) 415
SsiI CCGC 2 cut(s) 222, 225
SspMI CTAG 2 cut(s) 32, 128
TaaI ACNGT 4 cut(s) 161, 212, 331, 385
TaiI ACGT 3 cut(s) 274, 324, 349
TaqI TCGA 2 cut(s) 312, 326
TasI AATT 1 cut(s) 415
TauI GCSGC 1 cut(s) 227
TfiI GAWTC 3 cut(s) 180, 309, 314
Tru1I TTAA 2 cut(s) 258, 423
Tru9I TTAA 2 cut(s) 258, 423
TscAI CASTG 1 cut(s) 310
TseFI GTSAC 1 cut(s) 137
Tsp45I GTSAC 1 cut(s) 137
TspRI CASTG 1 cut(s) 310
XagI CCTNNNNNAGG 1 cut(s) 396
XapI RAATTY 1 cut(s) 415
XceI RCATGY 1 cut(s) 138
XmiI GTMKAC 1 cut(s) 216
XspI CTAG 2 cut(s) 32, 128
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.