MD02G1061100.v1.1

Lachrymatory-factor synthase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0006645
Physical Location & Seq
Reverse (-)
4890840 .. 4891628
789 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1061100.v1.1.491

Sequence Viewer

Length: 507 bp
ATGGCACTTGAGAAACAATTGAAATGGGAAGGCAAGGCAAGTGCAGAGCTAAAAGGCCCCAAAGCAGAGCAAATTTGGCCTCTTTTGGAGGACTTCTTTGGCCTGCACGAGTGGTTCCCAACTCTCACAACTTGCCATGGTGTTGAAGGCACATCGGGGGTAGCTGGGTGCGTGCGGTTCTGTGCTGGGTTCAAGACTCCGGTTGATCCTAAAAGTGACCAGAACCAAGACCAAGAGAAGGTGAACTGGACTAAGCAGAAGCTGCTGACTATTGACCCAGCAAATATGACATATAGTTACTCTATCATAGATGGGAATGTTGGGTTTAACTCGTACATTTCGACCGTTCAAGTGGTGCCGAAGGACGCCGGGTGTACGATTGTGTGGAAGTATGAAGTTGAACCAGTGGAGGGATGGAGGCTGGAGGATCTGGACCTGTTTATTGGCACTGGCTTGCAAGTCATGGCTAGCAGAATGGAAGCCTCTCTCCAGCTCCAAGTTGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Polyketide_cyc2

Protein Analysis

169

Amino Acids

18.79

Weight (kDa)

4.8

Isoelectric Point (pI)

24.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Polyketide_cyc2 PF10604 21 - 141 9.9e-21 Polyketide cyclase / dehydrase and lipid transport
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 355
AciI CCGC 1 cut(s) 175
AclWI GGATC 2 cut(s) 200, 435
AcsI RAATTY 1 cut(s) 72
AcyI GRCGYC 1 cut(s) 366
AfaI GTAC 2 cut(s) 335, 376
AfiI CCNNNNNNNGG 2 cut(s) 238, 410
AgsI TTSAA 6 cut(s) 22, 146, 193, 350, 401, 503
AluBI AGCT 4 cut(s) 49, 164, 262, 493
AluI AGCT 4 cut(s) 49, 164, 262, 493
AlwI GGATC 2 cut(s) 200, 435
AlwNI CAGNNNCTG 1 cut(s) 262
AoxI GGCC 3 cut(s) 55, 77, 100
ApeKI GCWGC 1 cut(s) 262
ApoI RAATTY 1 cut(s) 72
AspS9I GGNCC 2 cut(s) 56, 433
AsuC2I CCSGG 1 cut(s) 370
AsuHPI GGTGA 1 cut(s) 253
AsuNHI GCTAGC 1 cut(s) 467
AvaII GGWCC 1 cut(s) 433
BanI GGYRCC 1 cut(s) 355
BauI CACGAG 1 cut(s) 107
BbvI GCAGC 1 cut(s) 249
BccI CCATC 2 cut(s) 305, 408
BcnI CCSGG 1 cut(s) 370
BfaI CTAG 1 cut(s) 468
BisI GCNGC 1 cut(s) 263
BlsI GCNGC 1 cut(s) 264
Bme1390I CCNGG 1 cut(s) 370
Bme18I GGWCC 1 cut(s) 433
BmgT120I GGNCC 2 cut(s) 56, 433
BmiI GGNNCC 3 cut(s) 58, 116, 357
BmrFI CCNGG 1 cut(s) 370
BmtI GCTAGC 1 cut(s) 471
BpmI CTGGAG 2 cut(s) 443, 473
BpuEI CTTGAG 1 cut(s) 29
BpuMI CCSGG 1 cut(s) 370
BsaHI GRCGYC 1 cut(s) 366
BsaJI CCNNGG 1 cut(s) 136
BsaWI WCCGGW 1 cut(s) 199
Bsc4I CCNNNNNNNGG 2 cut(s) 238, 410
Bse1I ACTGG 3 cut(s) 251, 404, 454
BseDI CCNNGG 1 cut(s) 136
BseGI GGATG 1 cut(s) 419
BseLI CCNNNNNNNGG 2 cut(s) 238, 410
BseNI ACTGG 3 cut(s) 251, 404, 454
BseXI GCAGC 1 cut(s) 249
BseYI CCCAGC 3 cut(s) 164, 185, 277
BsgI GTGCAG 2 cut(s) 63, 89
Bsh1285I CGRYCG 1 cut(s) 345
BshFI GGCC 3 cut(s) 57, 79, 102
BshNI GGYRCC 1 cut(s) 355
BsiEI CGRYCG 1 cut(s) 345
BsiSI CCGG 2 cut(s) 200, 369
BslI CCNNNNNNNGG 2 cut(s) 238, 410
BsnI GGCC 3 cut(s) 57, 79, 102
Bsp143I GATC 2 cut(s) 205, 427
Bsp19I CCATGG 1 cut(s) 136
BspACI CCGC 1 cut(s) 175
BspANI GGCC 3 cut(s) 57, 79, 102
BspLI GGNNCC 3 cut(s) 58, 116, 357
BspOI GCTAGC 1 cut(s) 471
BspPI GGATC 2 cut(s) 200, 435
BspT107I GGYRCC 1 cut(s) 355
BsrI ACTGG 3 cut(s) 251, 404, 454
BssECI CCNNGG 1 cut(s) 136
BssMI GATC 2 cut(s) 205, 427
BssNI GRCGYC 1 cut(s) 366
BssSI CACGAG 1 cut(s) 107
BssT1I CCWWGG 1 cut(s) 136
Bst2BI CACGAG 1 cut(s) 107
Bst4CI ACNGT 1 cut(s) 346
BstACI GRCGYC 1 cut(s) 366
BstAPI GCANNNNNTGC 1 cut(s) 262
BstC8I GCNNGC 4 cut(s) 104, 173, 455, 469
BstDEI CTNAG 1 cut(s) 252
BstDSI CCRYGG 1 cut(s) 136
BstF5I GGATG 1 cut(s) 419
BstKTI GATC 2 cut(s) 208, 430
BstMBI GATC 2 cut(s) 205, 427
BstMCI CGRYCG 1 cut(s) 345
BstMWI GCNNNNNNNGC 2 cut(s) 76, 262
BstSCI CCNGG 1 cut(s) 368
BstV1I GCAGC 1 cut(s) 249
BstX2I RGATCY 1 cut(s) 427
BstYI RGATCY 1 cut(s) 427
BsuRI GGCC 3 cut(s) 57, 79, 102
BtgI CCRYGG 1 cut(s) 136
BtsCI GGATG 1 cut(s) 419
BtsIMutI CAGTG 2 cut(s) 411, 447
Cac8I GCNNGC 4 cut(s) 104, 173, 455, 469
CaiI CAGNNNCTG 1 cut(s) 262
Cfr13I GGNCC 2 cut(s) 56, 433
CseI GACGC 1 cut(s) 374
Csp6I GTAC 2 cut(s) 334, 375
CviAII CATG 2 cut(s) 137, 463
CviQI GTAC 2 cut(s) 334, 375
DdeI CTNAG 1 cut(s) 252
DpnI GATC 2 cut(s) 207, 429
DpnII GATC 2 cut(s) 205, 427
Eco130I CCWWGG 1 cut(s) 136
Eco47I GGWCC 1 cut(s) 433
EcoO109I RGGNCCY 1 cut(s) 56
EcoT14I CCWWGG 1 cut(s) 136
ErhI CCWWGG 1 cut(s) 136
FaeI CATG 2 cut(s) 140, 466
FaiI YATR 7 cut(s) 138, 287, 292, 294, 308, 393, 464
FatI CATG 2 cut(s) 136, 462
Fnu4HI GCNGC 1 cut(s) 263
FokI GGATG 1 cut(s) 426
Fsp4HI GCNGC 1 cut(s) 263
FspBI CTAG 1 cut(s) 468
GluI GCNGC 1 cut(s) 263
GsaI CCCAGC 3 cut(s) 168, 189, 281
GsuI CTGGAG 2 cut(s) 443, 473
HaeIII GGCC 3 cut(s) 57, 79, 102
HapII CCGG 2 cut(s) 200, 369
HgaI GACGC 1 cut(s) 374
Hin1I GRCGYC 1 cut(s) 366
Hin1II CATG 2 cut(s) 140, 466
HinfI GANTC 1 cut(s) 196
HpaII CCGG 2 cut(s) 200, 369
HphI GGTGA 1 cut(s) 253
Hpy166II GTNNAC 2 cut(s) 244, 375
Hpy188III TCNNGA 2 cut(s) 193, 431
Hpy8I GTNNAC 2 cut(s) 244, 375
HpyAV CCTTC 4 cut(s) 23, 140, 232, 355
HpyCH4III ACNGT 1 cut(s) 346
HpyCH4V TGCA 3 cut(s) 44, 106, 457
HpyF10VI GCNNNNNNNGC 2 cut(s) 76, 262
HpyF3I CTNAG 1 cut(s) 252
Hsp92I GRCGYC 1 cut(s) 366
Hsp92II CATG 2 cut(s) 140, 466
Kzo9I GATC 2 cut(s) 205, 427
LmnI GCTCC 1 cut(s) 498
Lsp1109I GCAGC 1 cut(s) 249
MaeI CTAG 1 cut(s) 468
MaeIII GTNAC 2 cut(s) 215, 296
MalI GATC 2 cut(s) 207, 429
MboI GATC 2 cut(s) 205, 427
MfeI CAATTG 1 cut(s) 17
MflI RGATCY 1 cut(s) 427
MluCI AATT 2 cut(s) 17, 72
MlyI GAGTC 1 cut(s) 190
MnlI CCTC 6 cut(s) 82, 90, 403, 411, 418, 493
MseI TTAA 1 cut(s) 327
MspI CCGG 2 cut(s) 200, 369
MspR9I CCNGG 1 cut(s) 370
MunI CAATTG 1 cut(s) 17
MwoI GCNNNNNNNGC 2 cut(s) 76, 262
NciI CCSGG 1 cut(s) 370
NcoI CCATGG 1 cut(s) 136
NdeII GATC 2 cut(s) 205, 427
NheI GCTAGC 1 cut(s) 467
NlaIII CATG 2 cut(s) 140, 466
NlaIV GGNNCC 3 cut(s) 58, 116, 357
NmuCI GTSAC 1 cut(s) 215
PcsI WCGNNNNNNNCGW 1 cut(s) 338
PkrI GCNGC 1 cut(s) 264
PleI GAGTC 1 cut(s) 190
PpsI GAGTC 1 cut(s) 190
PspFI CCCAGC 3 cut(s) 164, 185, 277
PspN4I GGNNCC 3 cut(s) 58, 116, 357
PspPI GGNCC 2 cut(s) 56, 433
PstNI CAGNNNCTG 1 cut(s) 262
PsuI RGATCY 1 cut(s) 427
RsaI GTAC 2 cut(s) 335, 376
RsaNI GTAC 2 cut(s) 334, 375
SaqAI TTAA 1 cut(s) 327
SatI GCNGC 1 cut(s) 263
Sau3AI GATC 2 cut(s) 205, 427
Sau96I GGNCC 2 cut(s) 56, 433
SchI GAGTC 1 cut(s) 190
ScrFI CCNGG 1 cut(s) 370
SetI ASST 6 cut(s) 51, 166, 243, 264, 438, 495
SinI GGWCC 1 cut(s) 433
SmlI CTYRAG 1 cut(s) 8
SmoI CTYRAG 1 cut(s) 8
Sse9I AATT 2 cut(s) 17, 72
SsiI CCGC 1 cut(s) 175
SspMI CTAG 1 cut(s) 468
StyD4I CCNGG 1 cut(s) 368
StyI CCWWGG 1 cut(s) 136
TaaI ACNGT 1 cut(s) 346
TaqI TCGA 1 cut(s) 341
TasI AATT 2 cut(s) 17, 72
Tru1I TTAA 1 cut(s) 327
Tru9I TTAA 1 cut(s) 327
TscAI CASTG 2 cut(s) 411, 454
TseFI GTSAC 1 cut(s) 215
TseI GCWGC 1 cut(s) 262
Tsp45I GTSAC 1 cut(s) 215
TspDTI ATGAA 1 cut(s) 408
TspRI CASTG 2 cut(s) 411, 454
VpaK11BI GGWCC 1 cut(s) 433
XapI RAATTY 1 cut(s) 72
XcmI CCANNNNNNNNNTGG 1 cut(s) 411
XspI CTAG 1 cut(s) 468
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.