Rw2G005630

Lachrymatory-factor synthase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0006645
Physical Location & Seq
Forward (+)
5359112 .. 5360100
989 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G005630.1

Sequence Viewer

Length: 576 bp
ATGCTCGGCCTCTTAGAAGAGATATCCTTGAACCAAGCTTCACTTGTTAGCTCTCAAATGGAACCTACTACCCAAATGGCTCATGACCAGCAACAACCAAAATGGGAAGGCGAGGCAAGGGCAGAGCTAATAGGCCCTAAAGCAGAACAAGTGTGGCCTTTGTTGGAGGACTTCTTCGGCCTACACAAGTGGTTCCCAACCCTAACCACTTGCCTTCCTGTAAAAGGCGTATCCGGTCAACCAGGGTGCGTGCGCTATTGCGCCGGATTCAAAACTCCGGTTGATCATGAAAGCAACCAGCCGGAGCATCTGAACTGGACTAAGCAGGAGCTACTGTCCATAGACCCAAGCCAGCTGACGTTTAGCTATTCCATTGTAGACGGGAATGTCGGGTTCAACTCGTACATTTCGACTGTGAGAGTGGTACCGAAGGAAGATGGATGCAGCATTGAGTGGAAGTATGAGGTTGAGCCAGTGGAGGGGTGGACACTGAAGGATCTGGACGTGTTTATAGGCACTGGTTTGCAAGTCATGGCTAGCAGAATGGAAGCTAGCCTCTCCAAACTCCAAGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Polyketide_cyc2

Protein Analysis

191

Amino Acids

21.37

Weight (kDa)

4.74

Isoelectric Point (pI)

34.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Polyketide_cyc2 PF10604 47 - 166 1.1e-19 Polyketide cyclase / dehydrase and lipid transport
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 386
Acc65I GGTACC 1 cut(s) 424
AccB1I GGYRCC 1 cut(s) 424
AccI GTMKAC 1 cut(s) 378
AclWI GGATC 1 cut(s) 504
AcuI CTGAAG 1 cut(s) 512
AfaI GTAC 2 cut(s) 404, 426
AfiI CCNNNNNNNGG 2 cut(s) 224, 479
AflIII ACRYGT 1 cut(s) 504
AgsI TTSAA 3 cut(s) 31, 271, 397
AjiI CACGTC 1 cut(s) 505
AjnI CCWGG 1 cut(s) 241
AluBI AGCT 7 cut(s) 38, 51, 127, 331, 355, 366, 551
AluI AGCT 7 cut(s) 38, 51, 127, 331, 355, 366, 551
AlwI GGATC 1 cut(s) 504
AoxI GGCC 4 cut(s) 7, 133, 155, 178
ApeKI GCWGC 1 cut(s) 444
Asp718I GGTACC 1 cut(s) 424
AspLEI GCGC 2 cut(s) 255, 263
AspS9I GGNCC 1 cut(s) 134
AsuNHI GCTAGC 2 cut(s) 536, 551
BanI GGYRCC 1 cut(s) 424
BbvI GCAGC 1 cut(s) 456
BccI CCATC 1 cut(s) 431
BciT130I CCWGG 1 cut(s) 243
BciVI GTATCC 1 cut(s) 241
BclI TGATCA 1 cut(s) 283
BfaI CTAG 2 cut(s) 537, 552
BfuI GTATCC 1 cut(s) 241
BisI GCNGC 1 cut(s) 445
BlsI GCNGC 1 cut(s) 446
Bme1390I CCNGG 1 cut(s) 243
BmgBI CACGTC 1 cut(s) 505
BmgT120I GGNCC 1 cut(s) 134
BmiI GGNNCC 3 cut(s) 63, 194, 426
BmrFI CCNGG 1 cut(s) 243
BmsI GCATC 2 cut(s) 316, 431
BmtI GCTAGC 2 cut(s) 540, 555
BsaJI CCNNGG 1 cut(s) 242
BsaWI WCCGGW 2 cut(s) 233, 277
BsaXI ACNNNNNCTCC 2 cut(s) 320, 350
Bsc4I CCNNNNNNNGG 2 cut(s) 224, 479
Bse1I ACTGG 3 cut(s) 320, 473, 523
BseBI CCWGG 1 cut(s) 243
BseDI CCNNGG 1 cut(s) 242
BseGI GGATG 1 cut(s) 446
BseLI CCNNNNNNNGG 2 cut(s) 224, 479
BseNI ACTGG 3 cut(s) 320, 473, 523
BseXI GCAGC 1 cut(s) 456
BshFI GGCC 4 cut(s) 9, 135, 157, 180
BshNI GGYRCC 1 cut(s) 424
BsiSI CCGG 4 cut(s) 234, 264, 278, 302
BslI CCNNNNNNNGG 2 cut(s) 224, 479
BsnI GGCC 4 cut(s) 9, 135, 157, 180
Bsp143I GATC 2 cut(s) 283, 496
BspANI GGCC 4 cut(s) 9, 135, 157, 180
BspHI TCATGA 2 cut(s) 82, 286
BspLI GGNNCC 3 cut(s) 63, 194, 426
BspOI GCTAGC 2 cut(s) 540, 555
BspPI GGATC 1 cut(s) 504
BspT107I GGYRCC 1 cut(s) 424
BsrI ACTGG 3 cut(s) 320, 473, 523
BssECI CCNNGG 1 cut(s) 242
BssMI GATC 2 cut(s) 283, 496
Bst2UI CCWGG 1 cut(s) 243
Bst4CI ACNGT 2 cut(s) 336, 415
Bst6I CTCTTC 1 cut(s) 12
BstC8I GCNNGC 4 cut(s) 251, 353, 538, 553
BstDEI CTNAG 2 cut(s) 13, 321
BstENI CCTNNNNNAGG 1 cut(s) 222
BstF5I GGATG 1 cut(s) 446
BstHHI GCGC 2 cut(s) 255, 263
BstKTI GATC 2 cut(s) 286, 499
BstMBI GATC 2 cut(s) 283, 496
BstNI CCWGG 1 cut(s) 243
BstSCI CCNGG 1 cut(s) 241
BstV1I GCAGC 1 cut(s) 456
BstX2I RGATCY 1 cut(s) 496
BstYI RGATCY 1 cut(s) 496
BsuI GTATCC 1 cut(s) 241
BsuRI GGCC 4 cut(s) 9, 135, 157, 180
BtrI CACGTC 1 cut(s) 505
BtsCI GGATG 1 cut(s) 446
BtsIMutI CAGTG 3 cut(s) 480, 488, 516
Cac8I GCNNGC 4 cut(s) 251, 353, 538, 553
CciI TCATGA 2 cut(s) 82, 286
CfoI GCGC 2 cut(s) 255, 263
Cfr13I GGNCC 1 cut(s) 134
Csp6I GTAC 2 cut(s) 403, 425
CviAII CATG 3 cut(s) 83, 287, 532
CviQI GTAC 2 cut(s) 403, 425
DdeI CTNAG 2 cut(s) 13, 321
DpnI GATC 2 cut(s) 285, 498
DpnII GATC 2 cut(s) 283, 496
DrdI GACNNNNNNGTC 1 cut(s) 386
DseDI GACNNNNNNGTC 1 cut(s) 386
Eam1104I CTCTTC 1 cut(s) 12
EarI CTCTTC 1 cut(s) 12
Eco32I GATATC 1 cut(s) 24
Eco57I CTGAAG 1 cut(s) 512
EcoNI CCTNNNNNAGG 1 cut(s) 222
EcoO109I RGGNCCY 1 cut(s) 134
EcoRII CCWGG 1 cut(s) 241
EcoRV GATATC 1 cut(s) 24
FaeI CATG 3 cut(s) 86, 290, 535
FaiI YATR 6 cut(s) 84, 288, 341, 462, 512, 533
FalI AAGNNNNNCTT 2 cut(s) 27, 59
FatI CATG 3 cut(s) 82, 286, 531
FbaI TGATCA 1 cut(s) 283
FblI GTMKAC 1 cut(s) 378
Fnu4HI GCNGC 1 cut(s) 445
FokI GGATG 1 cut(s) 453
Fsp4HI GCNGC 1 cut(s) 445
FspBI CTAG 2 cut(s) 537, 552
GlaI GCGC 2 cut(s) 254, 262
GluI GCNGC 1 cut(s) 445
HaeIII GGCC 4 cut(s) 9, 135, 157, 180
HapII CCGG 4 cut(s) 234, 264, 278, 302
HhaI GCGC 2 cut(s) 255, 263
Hin1II CATG 3 cut(s) 86, 290, 535
Hin6I GCGC 2 cut(s) 253, 261
HinP1I GCGC 2 cut(s) 253, 261
HincII GTYRAC 1 cut(s) 239
HindII GTYRAC 1 cut(s) 239
HindIII AAGCTT 1 cut(s) 36
HinfI GANTC 1 cut(s) 267
HpaII CCGG 4 cut(s) 234, 264, 278, 302
Hpy166II GTNNAC 3 cut(s) 239, 379, 486
Hpy188I TCNGA 1 cut(s) 312
Hpy188III TCNNGA 3 cut(s) 83, 287, 500
Hpy8I GTNNAC 3 cut(s) 239, 379, 486
HpyAV CCTTC 4 cut(s) 101, 224, 424, 487
HpyCH4III ACNGT 2 cut(s) 336, 415
HpyCH4IV ACGT 2 cut(s) 359, 504
HpyCH4V TGCA 2 cut(s) 444, 526
HpyF3I CTNAG 2 cut(s) 13, 321
HpySE526I ACGT 2 cut(s) 359, 504
Hsp92II CATG 3 cut(s) 86, 290, 535
HspAI GCGC 2 cut(s) 253, 261
KpnI GGTACC 1 cut(s) 428
Ksp22I TGATCA 1 cut(s) 283
Kzo9I GATC 2 cut(s) 283, 496
LmnI GCTCC 2 cut(s) 304, 328
Lsp1109I GCAGC 1 cut(s) 456
LweI GCATC 2 cut(s) 316, 431
MaeI CTAG 2 cut(s) 537, 552
MaeII ACGT 2 cut(s) 359, 504
MalI GATC 2 cut(s) 285, 498
MboI GATC 2 cut(s) 283, 496
MboII GAAGA 3 cut(s) 29, 166, 446
MflI RGATCY 1 cut(s) 496
MmeI TCCRAC 1 cut(s) 144
MnlI CCTC 6 cut(s) 20, 106, 160, 457, 472, 566
MspA1I CMGCKG 1 cut(s) 355
MspI CCGG 4 cut(s) 234, 264, 278, 302
MspR9I CCNGG 1 cut(s) 243
MvaI CCWGG 1 cut(s) 243
NdeII GATC 2 cut(s) 283, 496
NheI GCTAGC 2 cut(s) 536, 551
NlaIII CATG 3 cut(s) 86, 290, 535
NlaIV GGNNCC 3 cut(s) 63, 194, 426
PagI TCATGA 2 cut(s) 82, 286
PcsI WCGNNNNNNNCGW 1 cut(s) 407
PfeI GAWTC 1 cut(s) 267
PkrI GCNGC 1 cut(s) 446
Psp6I CCWGG 1 cut(s) 241
PspGI CCWGG 1 cut(s) 241
PspN4I GGNNCC 3 cut(s) 63, 194, 426
PspPI GGNCC 1 cut(s) 134
PsuI RGATCY 1 cut(s) 496
PvuII CAGCTG 1 cut(s) 355
RsaI GTAC 2 cut(s) 404, 426
RsaNI GTAC 2 cut(s) 403, 425
SatI GCNGC 1 cut(s) 445
Sau3AI GATC 2 cut(s) 283, 496
Sau96I GGNCC 1 cut(s) 134
ScrFI CCNGG 1 cut(s) 243
SfaNI GCATC 2 cut(s) 316, 431
SspMI CTAG 2 cut(s) 537, 552
StyD4I CCNGG 1 cut(s) 241
TaaI ACNGT 2 cut(s) 336, 415
TaiI ACGT 2 cut(s) 362, 507
TaqI TCGA 1 cut(s) 410
TfiI GAWTC 1 cut(s) 267
TscAI CASTG 3 cut(s) 480, 495, 523
TseI GCWGC 1 cut(s) 444
TspDTI ATGAA 1 cut(s) 303
TspRI CASTG 3 cut(s) 480, 495, 523
XagI CCTNNNNNAGG 1 cut(s) 222
XcmI CCANNNNNNNNNTGG 1 cut(s) 480
XmiI GTMKAC 1 cut(s) 378
XspI CTAG 2 cut(s) 537, 552
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.