Rw2G005640

Lachrymatory-factor synthase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0006645
Physical Location & Seq
Forward (+)
5365178 .. 5365684
507 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G005640.1

Sequence Viewer

Length: 507 bp
ATGGAACATACTACCCAAATATTGTCTCATGACCAGCGACAACCGAAATGGGAAGGCAAGGCTACTGCAGAGCTACGAGGTCCAAAAGCAGAACAAGTGTGGCCTTTGCTGGAGGACTTCTTCGGCCTTCATAAGTGGTTCCCAACCCTCCCCACTTGCCTTCCTGTGCAAGGCGTATCCGGGCAGCCAGGCTGTGTGCGCTACTGCGCCGGAATTAAGACTCCGGTTGATCGCGAAAGCGACCGGCTAGAGCATGTGAACTGGTCTAAGCAGCAGCTACTGTCCATAGACCCAAGCCAGCTGAACTATAGCTATTCCATTATAGATGGGAATATTGGGTTCAACTCGTACATTGCAACCATACGAGTGTTGCCCAAGGAAGATGGGTGCAGCATTGTGTGGAAGTATGAGGTTGAGCCAATTGAGGGGTGGACACTGATGGATCTTGACATGCTTATCGGCCCTGGTTTGCAACTTATGCCTTATGGCCAGAAAAATGGAAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Polyketide_cyc2

Protein Analysis

168

Amino Acids

19.02

Weight (kDa)

5.33

Isoelectric Point (pI)

34.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Polyketide_cyc2 PF10604 29 - 146 6.7e-17 Polyketide cyclase / dehydrase and lipid transport
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 234
AclWI GGATC 1 cut(s) 450
AcoI YGGCCR 1 cut(s) 487
AfaI GTAC 1 cut(s) 350
AfiI CCNNNNNNNGG 2 cut(s) 170, 425
AgsI TTSAA 1 cut(s) 343
AjnI CCWGG 2 cut(s) 187, 463
AluBI AGCT 5 cut(s) 73, 277, 301, 312, 504
AluI AGCT 5 cut(s) 73, 277, 301, 312, 504
Alw26I GTCTC 1 cut(s) 30
AlwI GGATC 1 cut(s) 450
AlwNI CAGNNNCTG 1 cut(s) 280
AoxI GGCC 4 cut(s) 101, 124, 460, 487
ApeKI GCWGC 4 cut(s) 184, 271, 274, 390
AspLEI GCGC 2 cut(s) 201, 209
AspS9I GGNCC 2 cut(s) 80, 461
AsuC2I CCSGG 1 cut(s) 181
AvaII GGWCC 1 cut(s) 80
BalI TGGCCA 1 cut(s) 489
BbvI GCAGC 4 cut(s) 196, 283, 286, 402
BccI CCATC 3 cut(s) 320, 377, 433
BciT130I CCWGG 2 cut(s) 189, 465
BciVI GTATCC 1 cut(s) 187
BcnI CCSGG 1 cut(s) 181
BcoDI GTCTC 1 cut(s) 30
BfaI CTAG 2 cut(s) 248, 505
BfmI CTRYAG 2 cut(s) 66, 307
BfuI GTATCC 1 cut(s) 187
BisI GCNGC 4 cut(s) 185, 272, 275, 391
BlsI GCNGC 4 cut(s) 186, 273, 276, 392
Bme1390I CCNGG 3 cut(s) 181, 189, 465
Bme18I GGWCC 1 cut(s) 80
BmgT120I GGNCC 2 cut(s) 80, 461
BmiI GGNNCC 1 cut(s) 140
BmrFI CCNGG 3 cut(s) 181, 189, 465
BpmI CTGGAG 1 cut(s) 131
BpuMI CCSGG 1 cut(s) 181
BsaJI CCNNGG 2 cut(s) 375, 463
BsaWI WCCGGW 1 cut(s) 223
Bsc4I CCNNNNNNNGG 2 cut(s) 170, 425
Bse118I RCCGGY 1 cut(s) 243
Bse1I ACTGG 1 cut(s) 266
Bse3DI GCAATG 1 cut(s) 351
BseBI CCWGG 2 cut(s) 189, 465
BseDI CCNNGG 2 cut(s) 375, 463
BseLI CCNNNNNNNGG 2 cut(s) 170, 425
BseMI GCAATG 1 cut(s) 351
BseNI ACTGG 1 cut(s) 266
BseXI GCAGC 4 cut(s) 196, 283, 286, 402
BsgI GTGCAG 1 cut(s) 409
Bsh1236I CGCG 1 cut(s) 234
Bsh1285I CGRYCG 1 cut(s) 244
BshFI GGCC 4 cut(s) 103, 126, 462, 489
BsiEI CGRYCG 1 cut(s) 244
BsiSI CCGG 4 cut(s) 180, 210, 224, 244
BslI CCNNNNNNNGG 2 cut(s) 170, 425
BsmAI GTCTC 1 cut(s) 30
BsnI GGCC 4 cut(s) 103, 126, 462, 489
Bsp143I GATC 2 cut(s) 229, 442
Bsp68I TCGCGA 1 cut(s) 234
BspANI GGCC 4 cut(s) 103, 126, 462, 489
BspFNI CGCG 1 cut(s) 234
BspHI TCATGA 1 cut(s) 28
BspLI GGNNCC 1 cut(s) 140
BspMAI CTGCAG 1 cut(s) 70
BspPI GGATC 1 cut(s) 450
BsrDI GCAATG 1 cut(s) 351
BsrFI RCCGGY 1 cut(s) 243
BsrI ACTGG 1 cut(s) 266
BssAI RCCGGY 1 cut(s) 243
BssECI CCNNGG 2 cut(s) 375, 463
BssMI GATC 2 cut(s) 229, 442
BssT1I CCWWGG 1 cut(s) 375
Bst2UI CCWGG 2 cut(s) 189, 465
Bst4CI ACNGT 1 cut(s) 282
BstAPI GCANNNNNTGC 1 cut(s) 478
BstC8I GCNNGC 1 cut(s) 299
BstDEI CTNAG 1 cut(s) 267
BstENI CCTNNNNNAGG 1 cut(s) 168
BstFNI CGCG 1 cut(s) 234
BstHHI GCGC 2 cut(s) 201, 209
BstKTI GATC 2 cut(s) 232, 445
BstMAI GTCTC 1 cut(s) 30
BstMBI GATC 2 cut(s) 229, 442
BstMCI CGRYCG 1 cut(s) 244
BstMWI GCNNNNNNNGC 2 cut(s) 198, 478
BstNI CCWGG 2 cut(s) 189, 465
BstNSI RCATGY 2 cut(s) 257, 454
BstSCI CCNGG 3 cut(s) 179, 187, 463
BstSFI CTRYAG 2 cut(s) 66, 307
BstUI CGCG 1 cut(s) 234
BstV1I GCAGC 4 cut(s) 196, 283, 286, 402
BstX2I RGATCY 1 cut(s) 442
BstXI CCANNNNNNTGG 1 cut(s) 497
BstYI RGATCY 1 cut(s) 442
BsuI GTATCC 1 cut(s) 187
BsuRI GGCC 4 cut(s) 103, 126, 462, 489
BtsIMutI CAGTG 1 cut(s) 434
BtuMI TCGCGA 1 cut(s) 234
Cac8I GCNNGC 1 cut(s) 299
CaiI CAGNNNCTG 1 cut(s) 280
CciI TCATGA 1 cut(s) 28
CfoI GCGC 2 cut(s) 201, 209
Cfr10I RCCGGY 1 cut(s) 243
Cfr13I GGNCC 2 cut(s) 80, 461
Csp6I GTAC 1 cut(s) 349
CviAII CATG 3 cut(s) 29, 254, 451
CviQI GTAC 1 cut(s) 349
DdeI CTNAG 1 cut(s) 267
DpnI GATC 2 cut(s) 231, 444
DpnII GATC 2 cut(s) 229, 442
EaeI YGGCCR 1 cut(s) 487
Eco130I CCWWGG 1 cut(s) 375
Eco47I GGWCC 1 cut(s) 80
EcoNI CCTNNNNNAGG 1 cut(s) 168
EcoRII CCWGG 2 cut(s) 187, 463
EcoT14I CCWWGG 1 cut(s) 375
ErhI CCWWGG 1 cut(s) 375
FaeI CATG 3 cut(s) 32, 257, 454
FatI CATG 3 cut(s) 28, 253, 450
Fnu4HI GCNGC 4 cut(s) 185, 272, 275, 391
Fsp4HI GCNGC 4 cut(s) 185, 272, 275, 391
FspBI CTAG 2 cut(s) 248, 505
GlaI GCGC 2 cut(s) 200, 208
GluI GCNGC 4 cut(s) 185, 272, 275, 391
GsuI CTGGAG 1 cut(s) 131
HaeIII GGCC 4 cut(s) 103, 126, 462, 489
HapII CCGG 4 cut(s) 180, 210, 224, 244
HhaI GCGC 2 cut(s) 201, 209
Hin1II CATG 3 cut(s) 32, 257, 454
Hin6I GCGC 2 cut(s) 199, 207
HinP1I GCGC 2 cut(s) 199, 207
HinfI GANTC 1 cut(s) 220
HpaII CCGG 4 cut(s) 180, 210, 224, 244
Hpy166II GTNNAC 2 cut(s) 259, 432
Hpy188III TCNNGA 3 cut(s) 29, 233, 446
Hpy8I GTNNAC 2 cut(s) 259, 432
HpyAV CCTTC 3 cut(s) 47, 137, 170
HpyCH4III ACNGT 1 cut(s) 282
HpyCH4V TGCA 5 cut(s) 68, 169, 356, 390, 472
HpyF10VI GCNNNNNNNGC 2 cut(s) 198, 478
HpyF3I CTNAG 1 cut(s) 267
Hsp92II CATG 3 cut(s) 32, 257, 454
HspAI GCGC 2 cut(s) 199, 207
Kzo9I GATC 2 cut(s) 229, 442
Lsp1109I GCAGC 4 cut(s) 196, 283, 286, 402
MaeI CTAG 2 cut(s) 248, 505
MalI GATC 2 cut(s) 231, 444
MboI GATC 2 cut(s) 229, 442
MboII GAAGA 2 cut(s) 112, 392
MfeI CAATTG 1 cut(s) 420
MflI RGATCY 1 cut(s) 442
MlsI TGGCCA 1 cut(s) 489
MluCI AATT 2 cut(s) 213, 420
MluNI TGGCCA 1 cut(s) 489
MlyI GAGTC 1 cut(s) 214
MnlI CCTC 5 cut(s) 71, 106, 158, 403, 418
Mox20I TGGCCA 1 cut(s) 489
MscI TGGCCA 1 cut(s) 489
MseI TTAA 1 cut(s) 216
MslI CAYNNNNRTG 1 cut(s) 365
Msp20I TGGCCA 1 cut(s) 489
MspA1I CMGCKG 1 cut(s) 301
MspI CCGG 4 cut(s) 180, 210, 224, 244
MspR9I CCNGG 3 cut(s) 181, 189, 465
MunI CAATTG 1 cut(s) 420
MvaI CCWGG 2 cut(s) 189, 465
MvnI CGCG 1 cut(s) 234
MwoI GCNNNNNNNGC 2 cut(s) 198, 478
NciI CCSGG 1 cut(s) 181
NdeII GATC 2 cut(s) 229, 442
NlaIII CATG 3 cut(s) 32, 257, 454
NlaIV GGNNCC 1 cut(s) 140
NruI TCGCGA 1 cut(s) 234
NspI RCATGY 2 cut(s) 257, 454
PagI TCATGA 1 cut(s) 28
PkrI GCNGC 4 cut(s) 186, 273, 276, 392
PleI GAGTC 1 cut(s) 214
PpsI GAGTC 1 cut(s) 214
Psp6I CCWGG 2 cut(s) 187, 463
PspGI CCWGG 2 cut(s) 187, 463
PspN4I GGNNCC 1 cut(s) 140
PspPI GGNCC 2 cut(s) 80, 461
PstI CTGCAG 1 cut(s) 70
PstNI CAGNNNCTG 1 cut(s) 280
PsuI RGATCY 1 cut(s) 442
PvuII CAGCTG 1 cut(s) 301
RruI TCGCGA 1 cut(s) 234
RsaI GTAC 1 cut(s) 350
RsaNI GTAC 1 cut(s) 349
RseI CAYNNNNRTG 1 cut(s) 365
SaqAI TTAA 1 cut(s) 216
SatI GCNGC 4 cut(s) 185, 272, 275, 391
Sau3AI GATC 2 cut(s) 229, 442
Sau96I GGNCC 2 cut(s) 80, 461
SchI GAGTC 1 cut(s) 214
ScrFI CCNGG 3 cut(s) 181, 189, 465
SetI ASST 7 cut(s) 75, 82, 279, 303, 314, 414, 506
SfcI CTRYAG 2 cut(s) 66, 307
SinI GGWCC 1 cut(s) 80
SmiMI CAYNNNNRTG 1 cut(s) 365
Sse9I AATT 2 cut(s) 213, 420
SspI AATATT 2 cut(s) 21, 334
SspMI CTAG 2 cut(s) 248, 505
StyD4I CCNGG 3 cut(s) 179, 187, 463
StyI CCWWGG 1 cut(s) 375
TaaI ACNGT 1 cut(s) 282
TasI AATT 2 cut(s) 213, 420
Tru1I TTAA 1 cut(s) 216
Tru9I TTAA 1 cut(s) 216
TscAI CASTG 1 cut(s) 441
TseI GCWGC 4 cut(s) 184, 271, 274, 390
TspDTI ATGAA 1 cut(s) 119
TspRI CASTG 1 cut(s) 441
VpaK11BI GGWCC 1 cut(s) 80
XagI CCTNNNNNAGG 1 cut(s) 168
XceI RCATGY 2 cut(s) 257, 454
XcmI CCANNNNNNNNNTGG 1 cut(s) 426
XspI CTAG 2 cut(s) 248, 505
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.