pycom08g09310

Polyketide cyclase / dehydrase and lipid transport

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0006645
Physical Location & Seq
Forward (+)
7568728 .. 7569216
489 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g09310.1

Sequence Viewer

Length: 489 bp
ATGTGTCCATTTCAGCCCATCATCAGAGTGTCCTCAACCCTTTTCCACCAAACCTACCCTCCCTATCTCTCTCTCTCTCTCTCAGATCAAATGGAGCAGCACTCAGATGCAAAGTGGGAAGCCAAGGTTTCTGCAACCCTAAAAGATGCCACAGTGGACCAGATATGGCCTCTCTTCAAAGACTTCTTTAATTTCCACAGGTGGTTTCCAACCCTAGCCACTTGCCATGGGATTCACGGAGCCAACGGAGAACCCGGCTGTATCCGATATTGCTCGGGGTTCTCAATCCCCTCCAAGAGTGGCGAGAAGTCCGTCAGCTGGTCCAAGGAACGATTAATAGCCGTTGATGATGCTGACCATAGTCTGAGCTACGAGATCGTGGAGAGCAACATTGGGTTCAATTCGTACGTCGCGACGTTCAGGATTGTTCCGCGTGGGGATATTGATGGTCGAGATGGGTGCGTGATCGATGTGGTCCATAACCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Polyketide_cyc2

Protein Analysis

163

Amino Acids

18.24

Weight (kDa)

5.85

Isoelectric Point (pI)

22.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Polyketide_cyc2 PF10604 40 - 147 4.8e-16 Polyketide cyclase / dehydrase and lipid transport
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 413, 433
AciI CCGC 1 cut(s) 431
AfaI GTAC 1 cut(s) 407
AfiI CCNNNNNNNGG 1 cut(s) 318
AgsI TTSAA 2 cut(s) 178, 400
AluBI AGCT 2 cut(s) 318, 369
AluI AGCT 2 cut(s) 318, 369
Ama87I CYCGRG 1 cut(s) 274
AoxI GGCC 1 cut(s) 167
ApeKI GCWGC 1 cut(s) 97
AseI ATTAAT 1 cut(s) 335
AspS9I GGNCC 3 cut(s) 157, 321, 475
AsuC2I CCSGG 1 cut(s) 255
AvaI CYCGRG 1 cut(s) 274
AvaII GGWCC 3 cut(s) 157, 321, 475
BbvI GCAGC 1 cut(s) 109
BccI CCATC 3 cut(s) 26, 440, 449
BceAI ACGGC 1 cut(s) 326
BcgI CGANNNNNNTGC 2 cut(s) 441, 475
BciVI GTATCC 1 cut(s) 272
BcnI CCSGG 1 cut(s) 255
BfaI CTAG 1 cut(s) 215
BfuI GTATCC 1 cut(s) 272
BisI GCNGC 1 cut(s) 98
BlsI GCNGC 1 cut(s) 99
Bme1390I CCNGG 1 cut(s) 255
Bme18I GGWCC 3 cut(s) 157, 321, 475
BmeT110I CYCGRG 1 cut(s) 274
BmgT120I GGNCC 3 cut(s) 157, 321, 475
BmiI GGNNCC 1 cut(s) 241
BmrFI CCNGG 1 cut(s) 255
BmsI GCATC 3 cut(s) 97, 136, 340
BoxI GACNNNNGTC 1 cut(s) 360
BplI GAGNNNNNCTC 2 cut(s) 86, 118
BpuMI CCSGG 1 cut(s) 255
Bsa29I ATCGAT 1 cut(s) 468
BsaJI CCNNGG 3 cut(s) 123, 226, 324
BsaXI ACNNNNNCTCC 2 cut(s) 43, 73
Bsc4I CCNNNNNNNGG 1 cut(s) 318
BseCI ATCGAT 1 cut(s) 468
BseDI CCNNGG 3 cut(s) 123, 226, 324
BseLI CCNNNNNNNGG 1 cut(s) 318
BseMII CTCAG 3 cut(s) 96, 117, 356
BseXI GCAGC 1 cut(s) 109
Bsh1236I CGCG 2 cut(s) 413, 433
BshFI GGCC 1 cut(s) 169
BshVI ATCGAT 1 cut(s) 468
BsiHKCI CYCGRG 1 cut(s) 274
BsiSI CCGG 1 cut(s) 255
BsiWI CGTACG 1 cut(s) 405
BslI CCNNNNNNNGG 1 cut(s) 318
BsnI GGCC 1 cut(s) 169
BsoBI CYCGRG 1 cut(s) 274
Bsp143I GATC 3 cut(s) 85, 375, 465
Bsp19I CCATGG 1 cut(s) 226
Bsp68I TCGCGA 1 cut(s) 413
BspACI CCGC 1 cut(s) 431
BspANI GGCC 1 cut(s) 169
BspCNI CTCAG 3 cut(s) 95, 116, 357
BspDI ATCGAT 1 cut(s) 468
BspFNI CGCG 2 cut(s) 413, 433
BspLI GGNNCC 1 cut(s) 241
BssECI CCNNGG 3 cut(s) 123, 226, 324
BssMI GATC 3 cut(s) 85, 375, 465
BssT1I CCWWGG 3 cut(s) 123, 226, 324
Bst4CI ACNGT 1 cut(s) 154
Bst6I CTCTTC 1 cut(s) 179
BstDEI CTNAG 3 cut(s) 82, 103, 365
BstDSI CCRYGG 1 cut(s) 226
BstFNI CGCG 2 cut(s) 413, 433
BstKTI GATC 3 cut(s) 88, 378, 468
BstMBI GATC 3 cut(s) 85, 375, 465
BstPAI GACNNNNGTC 1 cut(s) 360
BstSCI CCNGG 1 cut(s) 253
BstUI CGCG 2 cut(s) 413, 433
BstV1I GCAGC 1 cut(s) 109
Bsu15I ATCGAT 1 cut(s) 468
BsuI GTATCC 1 cut(s) 272
BsuRI GGCC 1 cut(s) 169
BsuTUI ATCGAT 1 cut(s) 468
BtgI CCRYGG 1 cut(s) 226
BtsIMutI CAGTG 1 cut(s) 159
BtuMI TCGCGA 1 cut(s) 413
Cfr13I GGNCC 3 cut(s) 157, 321, 475
ClaI ATCGAT 1 cut(s) 468
Csp6I GTAC 1 cut(s) 406
CviAII CATG 1 cut(s) 227
CviJI RGCY 9 cut(s) 16, 122, 169, 218, 242, 258, 318, 341, 369
CviKI_1 RGCY 9 cut(s) 16, 122, 169, 218, 242, 258, 318, 341, 369
CviQI GTAC 1 cut(s) 406
DdeI CTNAG 3 cut(s) 82, 103, 365
DpnI GATC 3 cut(s) 87, 377, 467
DpnII GATC 3 cut(s) 85, 375, 465
Eam1104I CTCTTC 1 cut(s) 179
EarI CTCTTC 1 cut(s) 179
Eco130I CCWWGG 3 cut(s) 123, 226, 324
Eco47I GGWCC 3 cut(s) 157, 321, 475
Eco88I CYCGRG 1 cut(s) 274
EcoT14I CCWWGG 3 cut(s) 123, 226, 324
ErhI CCWWGG 3 cut(s) 123, 226, 324
FaeI CATG 1 cut(s) 230
FaiI YATR 4 cut(s) 166, 228, 360, 480
FatI CATG 1 cut(s) 226
Fnu4HI GCNGC 1 cut(s) 98
Fsp4HI GCNGC 1 cut(s) 98
FspBI CTAG 1 cut(s) 215
GluI GCNGC 1 cut(s) 98
HaeIII GGCC 1 cut(s) 169
HapII CCGG 1 cut(s) 255
Hin1II CATG 1 cut(s) 230
HinfI GANTC 1 cut(s) 232
HpaII CCGG 1 cut(s) 255
Hpy166II GTNNAC 1 cut(s) 157
Hpy188I TCNGA 5 cut(s) 26, 85, 106, 266, 366
Hpy188III TCNNGA 3 cut(s) 412, 421, 452
Hpy8I GTNNAC 1 cut(s) 157
Hpy99I CGWCG 2 cut(s) 413, 418
HpyCH4III ACNGT 1 cut(s) 154
HpyCH4IV ACGT 2 cut(s) 408, 416
HpyCH4V TGCA 2 cut(s) 110, 134
HpyF3I CTNAG 3 cut(s) 82, 103, 365
HpySE526I ACGT 2 cut(s) 408, 416
Hsp92II CATG 1 cut(s) 230
Kzo9I GATC 3 cut(s) 85, 375, 465
LmnI GCTCC 2 cut(s) 94, 239
LpnPI CCDG 5 cut(s) 173, 184, 268, 304, 406
Lsp1109I GCAGC 1 cut(s) 109
LweI GCATC 3 cut(s) 97, 136, 340
MaeI CTAG 1 cut(s) 215
MaeII ACGT 2 cut(s) 408, 416
MalI GATC 3 cut(s) 87, 377, 467
MboI GATC 3 cut(s) 85, 375, 465
MboII GAAGA 1 cut(s) 166
MluCI AATT 2 cut(s) 190, 400
MmeI TCCRAC 1 cut(s) 233
MnlI CCTC 4 cut(s) 43, 69, 180, 301
MseI TTAA 2 cut(s) 189, 335
MslI CAYNNNNRTG 2 cut(s) 26, 105
MspA1I CMGCKG 1 cut(s) 318
MspI CCGG 1 cut(s) 255
MspR9I CCNGG 1 cut(s) 255
MvnI CGCG 2 cut(s) 413, 433
NciI CCSGG 1 cut(s) 255
NcoI CCATGG 1 cut(s) 226
NdeII GATC 3 cut(s) 85, 375, 465
NlaIII CATG 1 cut(s) 230
NlaIV GGNNCC 1 cut(s) 241
NruI TCGCGA 1 cut(s) 413
PcsI WCGNNNNNNNCGW 1 cut(s) 410
PfeI GAWTC 1 cut(s) 232
Pfl23II CGTACG 1 cut(s) 405
PkrI GCNGC 1 cut(s) 99
PshAI GACNNNNGTC 1 cut(s) 360
PshBI ATTAAT 1 cut(s) 335
PspLI CGTACG 1 cut(s) 405
PspN4I GGNNCC 1 cut(s) 241
PspPI GGNCC 3 cut(s) 157, 321, 475
PvuII CAGCTG 1 cut(s) 318
RruI TCGCGA 1 cut(s) 413
RsaI GTAC 1 cut(s) 407
RsaNI GTAC 1 cut(s) 406
RseI CAYNNNNRTG 2 cut(s) 26, 105
SaqAI TTAA 2 cut(s) 189, 335
SatI GCNGC 1 cut(s) 98
Sau3AI GATC 3 cut(s) 85, 375, 465
Sau96I GGNCC 3 cut(s) 157, 321, 475
ScrFI CCNGG 1 cut(s) 255
SetI ASST 7 cut(s) 56, 129, 203, 320, 371, 411, 419
SfaNI GCATC 3 cut(s) 97, 136, 340
SinI GGWCC 3 cut(s) 157, 321, 475
SmiMI CAYNNNNRTG 2 cut(s) 26, 105
Sse9I AATT 2 cut(s) 190, 400
SsiI CCGC 1 cut(s) 431
SspMI CTAG 1 cut(s) 215
StyD4I CCNGG 1 cut(s) 253
StyI CCWWGG 3 cut(s) 123, 226, 324
TaaI ACNGT 1 cut(s) 154
TaiI ACGT 2 cut(s) 411, 419
TaqI TCGA 2 cut(s) 451, 468
TasI AATT 2 cut(s) 190, 400
TfiI GAWTC 1 cut(s) 232
Tru1I TTAA 2 cut(s) 189, 335
Tru9I TTAA 2 cut(s) 189, 335
TscAI CASTG 1 cut(s) 159
TseI GCWGC 1 cut(s) 97
TspGWI ACGGA 3 cut(s) 252, 261, 301
TspRI CASTG 1 cut(s) 159
VpaK11BI GGWCC 3 cut(s) 157, 321, 475
VspI ATTAAT 1 cut(s) 335
XspI CTAG 1 cut(s) 215
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.