Rroxscaffold_2G00149890

Lachrymatory-factor synthase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0006645
Physical Location & Seq
Forward (+)
87401828 .. 87402346
519 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00149890.1

Sequence Viewer

Length: 519 bp
ATGGAACCTACTACCCAAATGGCTCATGACCAGCAACAACCAAAATGGGAAGGCGAGGCTAGGGCAGAGCTAATAGGCCCTAAAGCAGAACAAGTGTGGCCTTTGTTGGAGGACTTCTTCGGCCTACACAAGTGGTTCCCAACCCTAACCACTTGCCTTCCTGTACAAGGCGTATCCGGTCAACCAGGGTGCGTGCGCTATTGCGCCGGATTCAAAACTCCGGTTGATCGTGAAAGCAACCAACCGGAGCATCTGAACTGGACTAAGCAGGAGCTACTGTCCATAGACCCAAGCCAGCTGACGTTTAGCTATTCCATTGTAGACGGGAATGTCGGGTTCAACTCGTACATTTCGACTGTGCGAGTGGTGCCGAAGGAAGATGGATGCAGCATTGAGTGGAAGTATGAGGTTGAGCCAGTGGAGGGGTGGACACTGAAGGATCTGGATGCGTTTATAGGCACTGGTTTGCAAGTCATGGCTAGCAGAATGGAAGCTAGCCTCTCCAAACTCCAAGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Polyketide_cyc2

Protein Analysis

172

Amino Acids

19.35

Weight (kDa)

4.77

Isoelectric Point (pI)

32.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Polyketide_cyc2 PF10604 28 - 151 3.7e-20 Polyketide cyclase / dehydrase and lipid transport
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 329
AccB1I GGYRCC 1 cut(s) 367
AccI GTMKAC 1 cut(s) 321
AclWI GGATC 1 cut(s) 447
AcuI CTGAAG 1 cut(s) 455
AfaI GTAC 2 cut(s) 165, 347
AfiI CCNNNNNNNGG 2 cut(s) 167, 422
AgsI TTSAA 2 cut(s) 214, 340
AjnI CCWGG 1 cut(s) 184
AluBI AGCT 5 cut(s) 70, 274, 298, 309, 494
AluI AGCT 5 cut(s) 70, 274, 298, 309, 494
AlwI GGATC 1 cut(s) 447
AoxI GGCC 3 cut(s) 76, 98, 121
ApeKI GCWGC 1 cut(s) 387
AspLEI GCGC 2 cut(s) 198, 206
AspS9I GGNCC 1 cut(s) 77
AsuNHI GCTAGC 2 cut(s) 479, 494
BanI GGYRCC 1 cut(s) 367
BbvI GCAGC 1 cut(s) 399
BccI CCATC 1 cut(s) 374
BciT130I CCWGG 1 cut(s) 186
BciVI GTATCC 1 cut(s) 184
BfaI CTAG 3 cut(s) 60, 480, 495
BfuI GTATCC 1 cut(s) 184
BisI GCNGC 1 cut(s) 388
BlsI GCNGC 1 cut(s) 389
Bme1390I CCNGG 1 cut(s) 186
BmgT120I GGNCC 1 cut(s) 77
BmiI GGNNCC 3 cut(s) 6, 137, 369
BmrFI CCNGG 1 cut(s) 186
BmsI GCATC 3 cut(s) 259, 374, 436
BmtI GCTAGC 2 cut(s) 483, 498
BsaJI CCNNGG 1 cut(s) 185
BsaWI WCCGGW 3 cut(s) 176, 220, 244
BsaXI ACNNNNNCTCC 2 cut(s) 263, 293
Bsc4I CCNNNNNNNGG 2 cut(s) 167, 422
Bse1I ACTGG 3 cut(s) 263, 416, 466
BseBI CCWGG 1 cut(s) 186
BseDI CCNNGG 1 cut(s) 185
BseGI GGATG 2 cut(s) 389, 451
BseLI CCNNNNNNNGG 2 cut(s) 167, 422
BseNI ACTGG 3 cut(s) 263, 416, 466
BseXI GCAGC 1 cut(s) 399
BshFI GGCC 3 cut(s) 78, 100, 123
BshNI GGYRCC 1 cut(s) 367
BsiSI CCGG 4 cut(s) 177, 207, 221, 245
BslI CCNNNNNNNGG 2 cut(s) 167, 422
BsnI GGCC 3 cut(s) 78, 100, 123
Bsp1407I TGTACA 1 cut(s) 163
Bsp143I GATC 2 cut(s) 226, 439
BspANI GGCC 3 cut(s) 78, 100, 123
BspHI TCATGA 1 cut(s) 25
BspLI GGNNCC 3 cut(s) 6, 137, 369
BspOI GCTAGC 2 cut(s) 483, 498
BspPI GGATC 1 cut(s) 447
BspT107I GGYRCC 1 cut(s) 367
BsrGI TGTACA 1 cut(s) 163
BsrI ACTGG 3 cut(s) 263, 416, 466
BssECI CCNNGG 1 cut(s) 185
BssMI GATC 2 cut(s) 226, 439
Bst2UI CCWGG 1 cut(s) 186
Bst4CI ACNGT 2 cut(s) 279, 358
BstAUI TGTACA 1 cut(s) 163
BstC8I GCNNGC 4 cut(s) 194, 296, 481, 496
BstDEI CTNAG 1 cut(s) 264
BstENI CCTNNNNNAGG 1 cut(s) 165
BstF5I GGATG 2 cut(s) 389, 451
BstHHI GCGC 2 cut(s) 198, 206
BstKTI GATC 2 cut(s) 229, 442
BstMBI GATC 2 cut(s) 226, 439
BstMWI GCNNNNNNNGC 1 cut(s) 367
BstNI CCWGG 1 cut(s) 186
BstSCI CCNGG 1 cut(s) 184
BstV1I GCAGC 1 cut(s) 399
BstX2I RGATCY 1 cut(s) 439
BstYI RGATCY 1 cut(s) 439
BsuI GTATCC 1 cut(s) 184
BsuRI GGCC 3 cut(s) 78, 100, 123
BtsCI GGATG 2 cut(s) 389, 451
BtsIMutI CAGTG 3 cut(s) 423, 431, 459
Cac8I GCNNGC 4 cut(s) 194, 296, 481, 496
CciI TCATGA 1 cut(s) 25
CfoI GCGC 2 cut(s) 198, 206
Cfr13I GGNCC 1 cut(s) 77
Csp6I GTAC 2 cut(s) 164, 346
CviAII CATG 2 cut(s) 26, 475
CviQI GTAC 2 cut(s) 164, 346
DdeI CTNAG 1 cut(s) 264
DpnI GATC 2 cut(s) 228, 441
DpnII GATC 2 cut(s) 226, 439
DrdI GACNNNNNNGTC 1 cut(s) 329
DseDI GACNNNNNNGTC 1 cut(s) 329
Eco57I CTGAAG 1 cut(s) 455
EcoNI CCTNNNNNAGG 1 cut(s) 165
EcoO109I RGGNCCY 1 cut(s) 77
EcoRII CCWGG 1 cut(s) 184
FaeI CATG 2 cut(s) 29, 478
FaiI YATR 5 cut(s) 27, 284, 405, 455, 476
FatI CATG 2 cut(s) 25, 474
FblI GTMKAC 1 cut(s) 321
Fnu4HI GCNGC 1 cut(s) 388
FokI GGATG 2 cut(s) 396, 458
Fsp4HI GCNGC 1 cut(s) 388
FspBI CTAG 3 cut(s) 60, 480, 495
GlaI GCGC 2 cut(s) 197, 205
GluI GCNGC 1 cut(s) 388
HaeIII GGCC 3 cut(s) 78, 100, 123
HapII CCGG 4 cut(s) 177, 207, 221, 245
HhaI GCGC 2 cut(s) 198, 206
Hin1II CATG 2 cut(s) 29, 478
Hin6I GCGC 2 cut(s) 196, 204
HinP1I GCGC 2 cut(s) 196, 204
HincII GTYRAC 1 cut(s) 182
HindII GTYRAC 1 cut(s) 182
HinfI GANTC 1 cut(s) 210
HpaII CCGG 4 cut(s) 177, 207, 221, 245
Hpy166II GTNNAC 3 cut(s) 182, 322, 429
Hpy188I TCNGA 1 cut(s) 255
Hpy188III TCNNGA 3 cut(s) 26, 230, 443
Hpy8I GTNNAC 3 cut(s) 182, 322, 429
HpyAV CCTTC 4 cut(s) 44, 167, 367, 430
HpyCH4III ACNGT 2 cut(s) 279, 358
HpyCH4IV ACGT 1 cut(s) 302
HpyCH4V TGCA 2 cut(s) 387, 469
HpyF10VI GCNNNNNNNGC 1 cut(s) 367
HpyF3I CTNAG 1 cut(s) 264
HpySE526I ACGT 1 cut(s) 302
Hsp92II CATG 2 cut(s) 29, 478
HspAI GCGC 2 cut(s) 196, 204
Kzo9I GATC 2 cut(s) 226, 439
LmnI GCTCC 2 cut(s) 247, 271
Lsp1109I GCAGC 1 cut(s) 399
LweI GCATC 3 cut(s) 259, 374, 436
MaeI CTAG 3 cut(s) 60, 480, 495
MaeII ACGT 1 cut(s) 302
MalI GATC 2 cut(s) 228, 441
MboI GATC 2 cut(s) 226, 439
MboII GAAGA 2 cut(s) 109, 389
MflI RGATCY 1 cut(s) 439
MmeI TCCRAC 1 cut(s) 87
MnlI CCTC 5 cut(s) 49, 103, 400, 415, 509
MspA1I CMGCKG 1 cut(s) 298
MspI CCGG 4 cut(s) 177, 207, 221, 245
MspR9I CCNGG 1 cut(s) 186
MvaI CCWGG 1 cut(s) 186
MwoI GCNNNNNNNGC 1 cut(s) 367
NdeII GATC 2 cut(s) 226, 439
NheI GCTAGC 2 cut(s) 479, 494
NlaIII CATG 2 cut(s) 29, 478
NlaIV GGNNCC 3 cut(s) 6, 137, 369
PagI TCATGA 1 cut(s) 25
PcsI WCGNNNNNNNCGW 1 cut(s) 350
PfeI GAWTC 1 cut(s) 210
PkrI GCNGC 1 cut(s) 389
Psp6I CCWGG 1 cut(s) 184
PspGI CCWGG 1 cut(s) 184
PspN4I GGNNCC 3 cut(s) 6, 137, 369
PspPI GGNCC 1 cut(s) 77
PsuI RGATCY 1 cut(s) 439
PvuII CAGCTG 1 cut(s) 298
RsaI GTAC 2 cut(s) 165, 347
RsaNI GTAC 2 cut(s) 164, 346
SatI GCNGC 1 cut(s) 388
Sau3AI GATC 2 cut(s) 226, 439
Sau96I GGNCC 1 cut(s) 77
ScrFI CCNGG 1 cut(s) 186
SetI ASST 8 cut(s) 10, 72, 276, 300, 305, 311, 411, 496
SfaNI GCATC 3 cut(s) 259, 374, 436
SspMI CTAG 3 cut(s) 60, 480, 495
StyD4I CCNGG 1 cut(s) 184
TaaI ACNGT 2 cut(s) 279, 358
TaiI ACGT 1 cut(s) 305
TaqI TCGA 1 cut(s) 353
TatI WGTACW 1 cut(s) 163
TfiI GAWTC 1 cut(s) 210
TscAI CASTG 3 cut(s) 423, 438, 466
TseI GCWGC 1 cut(s) 387
TspRI CASTG 3 cut(s) 423, 438, 466
XagI CCTNNNNNAGG 1 cut(s) 165
XcmI CCANNNNNNNNNTGG 1 cut(s) 423
XmiI GTMKAC 1 cut(s) 321
XspI CTAG 3 cut(s) 60, 480, 495
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.