RLG00000016238

Lachrymatory-factor synthase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0006645
Physical Location & Seq
Reverse (-)
4977846 .. 4978226
381 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000016238

Sequence Viewer

Length: 381 bp
ATGGCTCATGACCAGCAACAACCAAAATGGGAAGGCGAGGCTAGGGCAGAACTAGTAGGCCCTAAAGCAGAACAAGTGTGGCCTTTGTTGGAGGACTTCTTCGGCTTGCACAAGTGGTTCCCAACACTAACCACTTGCCTTCCTGTACAAGGCGTATCCGGTAAACCAGGGTGTGTGCGCTACTGCGCCGGATTCAAAACTCCGGTTGATCATGAAAGCAACCAGCCGGAGCATCTGAACTGGAATAAGCAGGAGCTACTGTCCATAGACCCAAGCCAGCTGACCTATAGCTATTCCATTACAGATGGGAATATTGGGTTCAACTCGTACATTGCAACCGTGCAAGTGTTGCCCAAGGAAGATGGTGCACCATTGAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Polyketide_cyc2

Protein Analysis

127

Amino Acids

14.14

Weight (kDa)

5.24

Isoelectric Point (pI)

39.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Polyketide_cyc2 PF10604 22 - 122 3e-11 Polyketide cyclase / dehydrase and lipid transport
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AfaI GTAC 2 cut(s) 147, 329
AfiI CCNNNNNNNGG 1 cut(s) 149
AgsI TTSAA 2 cut(s) 196, 322
AhlI ACTAGT 1 cut(s) 52
AjnI CCWGG 1 cut(s) 166
AluBI AGCT 3 cut(s) 256, 280, 291
AluI AGCT 3 cut(s) 256, 280, 291
Alw21I GWGCWC 1 cut(s) 370
Alw44I GTGCAC 1 cut(s) 366
AoxI GGCC 2 cut(s) 58, 80
ApaLI GTGCAC 1 cut(s) 366
AspLEI GCGC 2 cut(s) 180, 188
AspS9I GGNCC 1 cut(s) 59
BaeGI GKGCMC 1 cut(s) 370
Bbv12I GWGCWC 1 cut(s) 370
BccI CCATC 2 cut(s) 299, 356
BciT130I CCWGG 1 cut(s) 168
BciVI GTATCC 1 cut(s) 166
BclI TGATCA 1 cut(s) 208
BcuI ACTAGT 1 cut(s) 52
BfaI CTAG 2 cut(s) 42, 53
BfmI CTRYAG 1 cut(s) 286
BfuI GTATCC 1 cut(s) 166
Bme1390I CCNGG 1 cut(s) 168
BmgT120I GGNCC 1 cut(s) 59
BmiI GGNNCC 1 cut(s) 119
BmrFI CCNGG 1 cut(s) 168
BmsI GCATC 1 cut(s) 241
BsaJI CCNNGG 2 cut(s) 167, 354
BsaWI WCCGGW 2 cut(s) 158, 202
BsaXI ACNNNNNCTCC 2 cut(s) 245, 275
Bsc4I CCNNNNNNNGG 1 cut(s) 149
Bse1I ACTGG 1 cut(s) 245
Bse3DI GCAATG 1 cut(s) 330
BseBI CCWGG 1 cut(s) 168
BseDI CCNNGG 2 cut(s) 167, 354
BseLI CCNNNNNNNGG 1 cut(s) 149
BseMI GCAATG 1 cut(s) 330
BseNI ACTGG 1 cut(s) 245
BseSI GKGCMC 1 cut(s) 370
BshFI GGCC 2 cut(s) 60, 82
BsiHKAI GWGCWC 1 cut(s) 370
BsiSI CCGG 4 cut(s) 159, 189, 203, 227
BslI CCNNNNNNNGG 1 cut(s) 149
BsnI GGCC 2 cut(s) 60, 82
Bsp1286I GDGCHC 1 cut(s) 370
Bsp1407I TGTACA 1 cut(s) 145
Bsp143I GATC 1 cut(s) 208
BspANI GGCC 2 cut(s) 60, 82
BspHI TCATGA 2 cut(s) 7, 211
BspLI GGNNCC 1 cut(s) 119
BsrDI GCAATG 1 cut(s) 330
BsrGI TGTACA 1 cut(s) 145
BsrI ACTGG 1 cut(s) 245
BssECI CCNNGG 2 cut(s) 167, 354
BssMI GATC 1 cut(s) 208
BssT1I CCWWGG 1 cut(s) 354
Bst2UI CCWGG 1 cut(s) 168
Bst4CI ACNGT 2 cut(s) 261, 340
BstAPI GCANNNNNTGC 1 cut(s) 349
BstAUI TGTACA 1 cut(s) 145
BstC8I GCNNGC 2 cut(s) 107, 278
BstENI CCTNNNNNAGG 1 cut(s) 147
BstHHI GCGC 2 cut(s) 180, 188
BstKTI GATC 1 cut(s) 211
BstMBI GATC 1 cut(s) 208
BstMWI GCNNNNNNNGC 1 cut(s) 349
BstNI CCWGG 1 cut(s) 168
BstSCI CCNGG 1 cut(s) 166
BstSFI CTRYAG 1 cut(s) 286
BstSLI GKGCMC 1 cut(s) 370
BsuI GTATCC 1 cut(s) 166
BsuRI GGCC 2 cut(s) 60, 82
Cac8I GCNNGC 2 cut(s) 107, 278
CciI TCATGA 2 cut(s) 7, 211
CfoI GCGC 2 cut(s) 180, 188
Cfr13I GGNCC 1 cut(s) 59
Csp6I GTAC 2 cut(s) 146, 328
CviAII CATG 2 cut(s) 8, 212
CviQI GTAC 2 cut(s) 146, 328
DpnI GATC 1 cut(s) 210
DpnII GATC 1 cut(s) 208
Eco130I CCWWGG 1 cut(s) 354
EcoNI CCTNNNNNAGG 1 cut(s) 147
EcoO109I RGGNCCY 1 cut(s) 59
EcoRII CCWGG 1 cut(s) 166
EcoT14I CCWWGG 1 cut(s) 354
ErhI CCWWGG 1 cut(s) 354
FaeI CATG 2 cut(s) 11, 215
FaiI YATR 4 cut(s) 9, 213, 266, 288
FatI CATG 2 cut(s) 7, 211
FbaI TGATCA 1 cut(s) 208
FspBI CTAG 2 cut(s) 42, 53
GlaI GCGC 2 cut(s) 179, 187
HaeIII GGCC 2 cut(s) 60, 82
HapII CCGG 4 cut(s) 159, 189, 203, 227
HhaI GCGC 2 cut(s) 180, 188
Hin1II CATG 2 cut(s) 11, 215
Hin6I GCGC 2 cut(s) 178, 186
HinP1I GCGC 2 cut(s) 178, 186
HinfI GANTC 1 cut(s) 192
HpaII CCGG 4 cut(s) 159, 189, 203, 227
Hpy166II GTNNAC 2 cut(s) 164, 368
Hpy188I TCNGA 1 cut(s) 237
Hpy188III TCNNGA 2 cut(s) 8, 212
Hpy8I GTNNAC 2 cut(s) 164, 368
HpyAV CCTTC 2 cut(s) 26, 149
HpyCH4III ACNGT 2 cut(s) 261, 340
HpyCH4V TGCA 4 cut(s) 109, 335, 343, 368
HpyF10VI GCNNNNNNNGC 1 cut(s) 349
Hsp92II CATG 2 cut(s) 11, 215
HspAI GCGC 2 cut(s) 178, 186
Ksp22I TGATCA 1 cut(s) 208
Kzo9I GATC 1 cut(s) 208
LmnI GCTCC 2 cut(s) 229, 253
LweI GCATC 1 cut(s) 241
MaeI CTAG 2 cut(s) 42, 53
MalI GATC 1 cut(s) 210
MboI GATC 1 cut(s) 208
MboII GAAGA 2 cut(s) 91, 371
MhlI GDGCHC 1 cut(s) 370
MmeI TCCRAC 1 cut(s) 69
MnlI CCTC 2 cut(s) 31, 85
MslI CAYNNNNRTG 1 cut(s) 376
MspA1I CMGCKG 1 cut(s) 280
MspI CCGG 4 cut(s) 159, 189, 203, 227
MspR9I CCNGG 1 cut(s) 168
MvaI CCWGG 1 cut(s) 168
MwoI GCNNNNNNNGC 1 cut(s) 349
NdeII GATC 1 cut(s) 208
NlaIII CATG 2 cut(s) 11, 215
NlaIV GGNNCC 1 cut(s) 119
PagI TCATGA 2 cut(s) 7, 211
PfeI GAWTC 1 cut(s) 192
Psp6I CCWGG 1 cut(s) 166
PspGI CCWGG 1 cut(s) 166
PspN4I GGNNCC 1 cut(s) 119
PspPI GGNCC 1 cut(s) 59
PvuII CAGCTG 1 cut(s) 280
RsaI GTAC 2 cut(s) 147, 329
RsaNI GTAC 2 cut(s) 146, 328
RseI CAYNNNNRTG 1 cut(s) 376
Sau3AI GATC 1 cut(s) 208
Sau96I GGNCC 1 cut(s) 59
ScrFI CCNGG 1 cut(s) 168
SduI GDGCHC 1 cut(s) 370
SetI ASST 4 cut(s) 258, 282, 287, 293
SfaNI GCATC 1 cut(s) 241
SfcI CTRYAG 1 cut(s) 286
SmiMI CAYNNNNRTG 1 cut(s) 376
SpeI ACTAGT 1 cut(s) 52
SspI AATATT 1 cut(s) 313
SspMI CTAG 2 cut(s) 42, 53
StyD4I CCNGG 1 cut(s) 166
StyI CCWWGG 1 cut(s) 354
TaaI ACNGT 2 cut(s) 261, 340
TatI WGTACW 1 cut(s) 145
TfiI GAWTC 1 cut(s) 192
TspDTI ATGAA 1 cut(s) 228
VneI GTGCAC 1 cut(s) 366
XagI CCTNNNNNAGG 1 cut(s) 147
XspI CTAG 2 cut(s) 42, 53
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.