MD02G1061300.v1.1

Lachrymatory-factor synthase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0006645
Physical Location & Seq
Forward (+)
4908045 .. 4908395
351 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1061300.v1.1.491

Sequence Viewer

Length: 351 bp
ATGGCACTTGAGAAACAATTGAAATGGGAAGGCAAGGCAAGTGCAGAGCTAAAAGTGACCAGAACTTGCCATGGTGTTGAAGGCACACCGGGGGTAGCCGGGTGCGTGTGGTTCTATGCTGGGTTCAAGACTCCGGTTGATCCTAAAAGTGACCAGAACCAAGACCAAGAGAAGGCGAACCGGACTAAGCAGAAGCTGCTGAGTATAGACCCAGCAGAAATGACATATAGTTACTCTATCATAGATGGGAATGTTGGGTTTAACTCGTACCTTTCGACCGTTCAAATGGTGCCGAAGGACGCCGAGTGTACGATTGTGTGGAAGTATGAAGTTGAACCAGTGGAGGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Polyketide_cyc2

Protein Analysis

117

Amino Acids

12.91

Weight (kDa)

5.15

Isoelectric Point (pI)

27.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Polyketide_cyc2 PF10604 21 - 113 4e-09 Polyketide cyclase / dehydrase and lipid transport
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 289
AclWI GGATC 1 cut(s) 134
AcyI GRCGYC 1 cut(s) 300
AfaI GTAC 2 cut(s) 269, 310
AfiI CCNNNNNNNGG 2 cut(s) 172, 344
AgsI TTSAA 5 cut(s) 22, 80, 127, 284, 335
AluBI AGCT 2 cut(s) 49, 196
AluI AGCT 2 cut(s) 49, 196
AlwI GGATC 1 cut(s) 134
AlwNI CAGNNNCTG 1 cut(s) 196
ApeKI GCWGC 1 cut(s) 196
AsuC2I CCSGG 2 cut(s) 90, 100
BanI GGYRCC 1 cut(s) 289
BbvI GCAGC 1 cut(s) 183
BccI CCATC 1 cut(s) 239
BcnI CCSGG 2 cut(s) 90, 100
BisI GCNGC 1 cut(s) 197
BlsI GCNGC 1 cut(s) 198
Bme1390I CCNGG 2 cut(s) 90, 100
BmiI GGNNCC 1 cut(s) 291
BmrFI CCNGG 2 cut(s) 90, 100
BpuEI CTTGAG 1 cut(s) 29
BpuMI CCSGG 2 cut(s) 90, 100
BsaHI GRCGYC 1 cut(s) 300
BsaJI CCNNGG 2 cut(s) 70, 89
BsaWI WCCGGW 2 cut(s) 133, 180
Bsc4I CCNNNNNNNGG 2 cut(s) 172, 344
Bse1I ACTGG 1 cut(s) 338
BseDI CCNNGG 2 cut(s) 70, 89
BseLI CCNNNNNNNGG 2 cut(s) 172, 344
BseMII CTCAG 1 cut(s) 191
BseNI ACTGG 1 cut(s) 338
BseXI GCAGC 1 cut(s) 183
BseYI CCCAGC 2 cut(s) 119, 211
BsgI GTGCAG 1 cut(s) 63
Bsh1285I CGRYCG 1 cut(s) 279
BshNI GGYRCC 1 cut(s) 289
BsiEI CGRYCG 1 cut(s) 279
BsiSI CCGG 4 cut(s) 89, 99, 134, 181
BslI CCNNNNNNNGG 2 cut(s) 172, 344
Bsp143I GATC 1 cut(s) 139
Bsp19I CCATGG 1 cut(s) 70
BspCNI CTCAG 1 cut(s) 192
BspLI GGNNCC 1 cut(s) 291
BspPI GGATC 1 cut(s) 134
BspT107I GGYRCC 1 cut(s) 289
BsrI ACTGG 1 cut(s) 338
BssECI CCNNGG 2 cut(s) 70, 89
BssMI GATC 1 cut(s) 139
BssNI GRCGYC 1 cut(s) 300
BssT1I CCWWGG 1 cut(s) 70
Bst4CI ACNGT 1 cut(s) 280
BstACI GRCGYC 1 cut(s) 300
BstAPI GCANNNNNTGC 1 cut(s) 196
BstDEI CTNAG 2 cut(s) 186, 200
BstDSI CCRYGG 1 cut(s) 70
BstKTI GATC 1 cut(s) 142
BstMBI GATC 1 cut(s) 139
BstMCI CGRYCG 1 cut(s) 279
BstMWI GCNNNNNNNGC 1 cut(s) 196
BstSCI CCNGG 2 cut(s) 88, 98
BstV1I GCAGC 1 cut(s) 183
BtgI CCRYGG 1 cut(s) 70
BtsIMutI CAGTG 1 cut(s) 345
CaiI CAGNNNCTG 1 cut(s) 196
CseI GACGC 1 cut(s) 308
Csp6I GTAC 2 cut(s) 268, 309
CviAII CATG 1 cut(s) 71
CviJI RGCY 3 cut(s) 49, 98, 196
CviKI_1 RGCY 3 cut(s) 49, 98, 196
CviQI GTAC 2 cut(s) 268, 309
DdeI CTNAG 2 cut(s) 186, 200
DpnI GATC 1 cut(s) 141
DpnII GATC 1 cut(s) 139
Eco130I CCWWGG 1 cut(s) 70
EcoT14I CCWWGG 1 cut(s) 70
ErhI CCWWGG 1 cut(s) 70
FaeI CATG 1 cut(s) 74
FaiI YATR 7 cut(s) 72, 117, 206, 226, 228, 242, 327
FatI CATG 1 cut(s) 70
Fnu4HI GCNGC 1 cut(s) 197
Fsp4HI GCNGC 1 cut(s) 197
GluI GCNGC 1 cut(s) 197
GsaI CCCAGC 2 cut(s) 123, 215
HapII CCGG 4 cut(s) 89, 99, 134, 181
HgaI GACGC 1 cut(s) 308
Hin1I GRCGYC 1 cut(s) 300
Hin1II CATG 1 cut(s) 74
HinfI GANTC 1 cut(s) 130
HpaII CCGG 4 cut(s) 89, 99, 134, 181
Hpy166II GTNNAC 1 cut(s) 309
Hpy188III TCNNGA 1 cut(s) 127
Hpy8I GTNNAC 1 cut(s) 309
HpyAV CCTTC 4 cut(s) 23, 74, 166, 289
HpyCH4III ACNGT 1 cut(s) 280
HpyCH4V TGCA 1 cut(s) 44
HpyF10VI GCNNNNNNNGC 1 cut(s) 196
HpyF3I CTNAG 2 cut(s) 186, 200
Hsp92I GRCGYC 1 cut(s) 300
Hsp92II CATG 1 cut(s) 74
Kzo9I GATC 1 cut(s) 139
LpnPI CCDG 8 cut(s) 73, 102, 105, 112, 147, 167, 194, 225
Lsp1109I GCAGC 1 cut(s) 183
MaeIII GTNAC 3 cut(s) 55, 149, 230
MalI GATC 1 cut(s) 141
MboI GATC 1 cut(s) 139
MfeI CAATTG 1 cut(s) 17
MluCI AATT 1 cut(s) 17
MlyI GAGTC 1 cut(s) 124
MnlI CCTC 1 cut(s) 337
MseI TTAA 1 cut(s) 261
MspI CCGG 4 cut(s) 89, 99, 134, 181
MspR9I CCNGG 2 cut(s) 90, 100
MunI CAATTG 1 cut(s) 17
MwoI GCNNNNNNNGC 1 cut(s) 196
NciI CCSGG 2 cut(s) 90, 100
NcoI CCATGG 1 cut(s) 70
NdeII GATC 1 cut(s) 139
NlaIII CATG 1 cut(s) 74
NlaIV GGNNCC 1 cut(s) 291
NmeAIII GCCGAG 1 cut(s) 328
NmuCI GTSAC 2 cut(s) 55, 149
PcsI WCGNNNNNNNCGW 1 cut(s) 272
PkrI GCNGC 1 cut(s) 198
PleI GAGTC 1 cut(s) 124
PpsI GAGTC 1 cut(s) 124
PspFI CCCAGC 2 cut(s) 119, 211
PspN4I GGNNCC 1 cut(s) 291
PstNI CAGNNNCTG 1 cut(s) 196
RsaI GTAC 2 cut(s) 269, 310
RsaNI GTAC 2 cut(s) 268, 309
SaqAI TTAA 1 cut(s) 261
SatI GCNGC 1 cut(s) 197
Sau3AI GATC 1 cut(s) 139
SchI GAGTC 1 cut(s) 124
ScrFI CCNGG 2 cut(s) 90, 100
SetI ASST 3 cut(s) 51, 198, 273
SmlI CTYRAG 1 cut(s) 8
SmoI CTYRAG 1 cut(s) 8
Sse9I AATT 1 cut(s) 17
StyD4I CCNGG 2 cut(s) 88, 98
StyI CCWWGG 1 cut(s) 70
TaaI ACNGT 1 cut(s) 280
TaqI TCGA 1 cut(s) 275
TasI AATT 1 cut(s) 17
Tru1I TTAA 1 cut(s) 261
Tru9I TTAA 1 cut(s) 261
TscAI CASTG 1 cut(s) 345
TseFI GTSAC 2 cut(s) 55, 149
TseI GCWGC 1 cut(s) 196
Tsp45I GTSAC 2 cut(s) 55, 149
TspDTI ATGAA 1 cut(s) 342
TspRI CASTG 1 cut(s) 345
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.