MD02G1064000.v1.1

Lachrymatory-factor synthase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0006645
Physical Location & Seq
Reverse (-)
5169266 .. 5169748
483 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1064000.v1.1.491

Sequence Viewer

Length: 483 bp
ATGGGAAGGCAAGGCAAGTGCAGAGCTAAAAGGCCCCAAAGCAGAGCAAATTTGGCCTCTTTTGGAGACTTCTTTGGCTTGCACAAGTGGTTCCCAACTCTCACAACTTGCCTTGGTGTTGAAGGCACACAGGGGGTAGCTGGGTGCTTGCGGTTCTGTGCTGGAGTCAAGACTCCGGTTGATCATAAAAGTGACCAGAACCAAGACCAAGAGAAGGTGAACTGGACTAAGCAGAAGCTGCTGAGTATAGACCCAGCAAAAATGACATATAGTTACTCTATCATAGATGGGAATATTGGGTTTAACTCGTACATTTCGACCGTTCAAGTGGTGCTGAAGGACGCCGGGTGTATGATTGTGTGGAAGTATGAAGTTGAACCAGTGGAGGGATTGAGGCTGGAGGATCTGGACCTGCTCATTGGCACTGGTTTGCAAGTCATGGCTAGCAGAATGGAAGCCTCTCTTGAACTCCAAGTTGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Polyketide_cyc2

Protein Analysis

161

Amino Acids

17.76

Weight (kDa)

6.73

Isoelectric Point (pI)

40.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Polyketide_cyc2 PF10604 21 - 131 3.2e-13 Polyketide cyclase / dehydrase and lipid transport
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 420
AciI CCGC 1 cut(s) 151
AclWI GGATC 1 cut(s) 411
AcsI RAATTY 1 cut(s) 49
AcuI CTGAAG 1 cut(s) 356
AcyI GRCGYC 1 cut(s) 342
AfaI GTAC 1 cut(s) 311
AfiI CCNNNNNNNGG 2 cut(s) 214, 386
AgsI TTSAA 5 cut(s) 122, 326, 377, 467, 479
AluBI AGCT 3 cut(s) 26, 140, 238
AluI AGCT 3 cut(s) 26, 140, 238
Alw26I GTCTC 1 cut(s) 60
AlwI GGATC 1 cut(s) 411
AlwNI CAGNNNCTG 1 cut(s) 238
AoxI GGCC 2 cut(s) 32, 54
ApeKI GCWGC 1 cut(s) 238
ApoI RAATTY 1 cut(s) 49
AspS9I GGNCC 2 cut(s) 33, 409
AsuC2I CCSGG 1 cut(s) 346
AsuHPI GGTGA 1 cut(s) 229
AsuNHI GCTAGC 1 cut(s) 443
AvaII GGWCC 1 cut(s) 409
BbvI GCAGC 1 cut(s) 225
BccI CCATC 1 cut(s) 281
BclI TGATCA 1 cut(s) 181
BcnI CCSGG 1 cut(s) 346
BcoDI GTCTC 1 cut(s) 60
BfaI CTAG 1 cut(s) 444
BfuAI ACCTGC 1 cut(s) 420
BisI GCNGC 1 cut(s) 239
BlsI GCNGC 1 cut(s) 240
Bme1390I CCNGG 1 cut(s) 346
Bme18I GGWCC 1 cut(s) 409
BmgT120I GGNCC 2 cut(s) 33, 409
BmiI GGNNCC 2 cut(s) 35, 92
BmrFI CCNGG 1 cut(s) 346
BmtI GCTAGC 1 cut(s) 447
BpmI CTGGAG 2 cut(s) 183, 419
BpuMI CCSGG 1 cut(s) 346
BsaHI GRCGYC 1 cut(s) 342
BsaJI CCNNGG 1 cut(s) 112
BsaWI WCCGGW 1 cut(s) 175
Bsc4I CCNNNNNNNGG 2 cut(s) 214, 386
Bse1I ACTGG 3 cut(s) 227, 380, 430
BseDI CCNNGG 1 cut(s) 112
BseLI CCNNNNNNNGG 2 cut(s) 214, 386
BseMII CTCAG 1 cut(s) 233
BseNI ACTGG 3 cut(s) 227, 380, 430
BseXI GCAGC 1 cut(s) 225
BseYI CCCAGC 2 cut(s) 140, 253
BsgI GTGCAG 1 cut(s) 40
Bsh1285I CGRYCG 1 cut(s) 321
BshFI GGCC 2 cut(s) 34, 56
BsiEI CGRYCG 1 cut(s) 321
BsiSI CCGG 2 cut(s) 176, 345
BslI CCNNNNNNNGG 2 cut(s) 214, 386
BsmAI GTCTC 1 cut(s) 60
BsnI GGCC 2 cut(s) 34, 56
Bsp143I GATC 2 cut(s) 181, 403
BspACI CCGC 1 cut(s) 151
BspANI GGCC 2 cut(s) 34, 56
BspCNI CTCAG 1 cut(s) 234
BspLI GGNNCC 2 cut(s) 35, 92
BspMI ACCTGC 1 cut(s) 420
BspOI GCTAGC 1 cut(s) 447
BspPI GGATC 1 cut(s) 411
BsrI ACTGG 3 cut(s) 227, 380, 430
BssECI CCNNGG 1 cut(s) 112
BssMI GATC 2 cut(s) 181, 403
BssNI GRCGYC 1 cut(s) 342
BssT1I CCWWGG 1 cut(s) 112
Bst4CI ACNGT 1 cut(s) 322
BstACI GRCGYC 1 cut(s) 342
BstAPI GCANNNNNTGC 1 cut(s) 238
BstC8I GCNNGC 3 cut(s) 80, 149, 445
BstDEI CTNAG 2 cut(s) 228, 242
BstKTI GATC 2 cut(s) 184, 406
BstMAI GTCTC 1 cut(s) 60
BstMBI GATC 2 cut(s) 181, 403
BstMCI CGRYCG 1 cut(s) 321
BstMWI GCNNNNNNNGC 2 cut(s) 53, 238
BstSCI CCNGG 1 cut(s) 344
BstV1I GCAGC 1 cut(s) 225
BstX2I RGATCY 1 cut(s) 403
BstYI RGATCY 1 cut(s) 403
BsuRI GGCC 2 cut(s) 34, 56
BtsIMutI CAGTG 2 cut(s) 387, 423
BveI ACCTGC 1 cut(s) 420
Cac8I GCNNGC 3 cut(s) 80, 149, 445
CaiI CAGNNNCTG 1 cut(s) 238
Cfr13I GGNCC 2 cut(s) 33, 409
CseI GACGC 1 cut(s) 350
Csp6I GTAC 1 cut(s) 310
CviAII CATG 1 cut(s) 439
CviJI RGCY 9 cut(s) 26, 34, 56, 78, 140, 238, 397, 443, 458
CviKI_1 RGCY 9 cut(s) 26, 34, 56, 78, 140, 238, 397, 443, 458
CviQI GTAC 1 cut(s) 310
DdeI CTNAG 2 cut(s) 228, 242
DpnI GATC 2 cut(s) 183, 405
DpnII GATC 2 cut(s) 181, 403
Eco130I CCWWGG 1 cut(s) 112
Eco47I GGWCC 1 cut(s) 409
Eco57I CTGAAG 1 cut(s) 356
EcoO109I RGGNCCY 1 cut(s) 33
EcoT14I CCWWGG 1 cut(s) 112
ErhI CCWWGG 1 cut(s) 112
FaeI CATG 1 cut(s) 442
FaiI YATR 8 cut(s) 186, 248, 268, 270, 284, 353, 369, 440
FalI AAGNNNNNCTT 2 cut(s) 447, 479
FatI CATG 1 cut(s) 438
FbaI TGATCA 1 cut(s) 181
Fnu4HI GCNGC 1 cut(s) 239
Fsp4HI GCNGC 1 cut(s) 239
FspBI CTAG 1 cut(s) 444
GluI GCNGC 1 cut(s) 239
GsaI CCCAGC 2 cut(s) 144, 257
GsuI CTGGAG 2 cut(s) 183, 419
HaeIII GGCC 2 cut(s) 34, 56
HapII CCGG 2 cut(s) 176, 345
HgaI GACGC 1 cut(s) 350
Hin1I GRCGYC 1 cut(s) 342
Hin1II CATG 1 cut(s) 442
HinfI GANTC 2 cut(s) 165, 172
HpaII CCGG 2 cut(s) 176, 345
HphI GGTGA 1 cut(s) 229
Hpy166II GTNNAC 1 cut(s) 220
Hpy188III TCNNGA 3 cut(s) 169, 407, 464
Hpy8I GTNNAC 1 cut(s) 220
HpyAV CCTTC 3 cut(s) 116, 208, 331
HpyCH4III ACNGT 1 cut(s) 322
HpyCH4V TGCA 3 cut(s) 21, 82, 433
HpyF10VI GCNNNNNNNGC 2 cut(s) 53, 238
HpyF3I CTNAG 2 cut(s) 228, 242
Hsp92I GRCGYC 1 cut(s) 342
Hsp92II CATG 1 cut(s) 442
Ksp22I TGATCA 1 cut(s) 181
Kzo9I GATC 2 cut(s) 181, 403
Lsp1109I GCAGC 1 cut(s) 225
MaeI CTAG 1 cut(s) 444
MaeIII GTNAC 2 cut(s) 191, 272
MalI GATC 2 cut(s) 183, 405
MboI GATC 2 cut(s) 181, 403
MflI RGATCY 1 cut(s) 403
MluCI AATT 1 cut(s) 49
MlyI GAGTC 2 cut(s) 166, 174
MnlI CCTC 5 cut(s) 67, 379, 387, 394, 469
MseI TTAA 1 cut(s) 303
MslI CAYNNNNRTG 1 cut(s) 189
MspI CCGG 2 cut(s) 176, 345
MspR9I CCNGG 1 cut(s) 346
MwoI GCNNNNNNNGC 2 cut(s) 53, 238
NciI CCSGG 1 cut(s) 346
NdeII GATC 2 cut(s) 181, 403
NheI GCTAGC 1 cut(s) 443
NlaIII CATG 1 cut(s) 442
NlaIV GGNNCC 2 cut(s) 35, 92
NmuCI GTSAC 1 cut(s) 191
PcsI WCGNNNNNNNCGW 1 cut(s) 314
PkrI GCNGC 1 cut(s) 240
PleI GAGTC 2 cut(s) 166, 173
PpsI GAGTC 2 cut(s) 166, 173
PspFI CCCAGC 2 cut(s) 140, 253
PspN4I GGNNCC 2 cut(s) 35, 92
PspPI GGNCC 2 cut(s) 33, 409
PstNI CAGNNNCTG 1 cut(s) 238
PsuI RGATCY 1 cut(s) 403
RsaI GTAC 1 cut(s) 311
RsaNI GTAC 1 cut(s) 310
RseI CAYNNNNRTG 1 cut(s) 189
SaqAI TTAA 1 cut(s) 303
SatI GCNGC 1 cut(s) 239
Sau3AI GATC 2 cut(s) 181, 403
Sau96I GGNCC 2 cut(s) 33, 409
SchI GAGTC 2 cut(s) 166, 174
ScrFI CCNGG 1 cut(s) 346
SetI ASST 5 cut(s) 28, 142, 219, 240, 414
SinI GGWCC 1 cut(s) 409
SmiMI CAYNNNNRTG 1 cut(s) 189
Sse9I AATT 1 cut(s) 49
SsiI CCGC 1 cut(s) 151
SspI AATATT 1 cut(s) 295
SspMI CTAG 1 cut(s) 444
StyD4I CCNGG 1 cut(s) 344
StyI CCWWGG 1 cut(s) 112
TaaI ACNGT 1 cut(s) 322
TaqI TCGA 1 cut(s) 317
TasI AATT 1 cut(s) 49
Tru1I TTAA 1 cut(s) 303
Tru9I TTAA 1 cut(s) 303
TscAI CASTG 2 cut(s) 387, 430
TseFI GTSAC 1 cut(s) 191
TseI GCWGC 1 cut(s) 238
Tsp45I GTSAC 1 cut(s) 191
TspDTI ATGAA 1 cut(s) 384
TspRI CASTG 2 cut(s) 387, 430
VpaK11BI GGWCC 1 cut(s) 409
XapI RAATTY 1 cut(s) 49
XspI CTAG 1 cut(s) 444
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.