FvH4_1g05920

Lachrymatory-factor synthase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0006645
Physical Location & Seq
Reverse (-)
3147087 .. 3147885
799 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g05920.t1

Sequence Viewer

Length: 507 bp
ATGGAACCTACTACCCAAATGCCTCATGACCAGCGGCAACCAAAATGGGAAGGCAAGGCTTGTGCAGAGCTACGAAGTCCAAAAGCAGAACAAGTGTGGCCTTTGCTGGAGGACTTCTTTGGCCTACACAAGTGGTTGCCAGCCCTCCCCACATGCCTTCCTGTGCAAGGGGTATCCGGGCAGCCAGGCTGCGTGCGCTACTGCGCTGGAGTCAAGACTCCAGTTGATCTCGAGAGTGACCAGCCGGCGCACTGGTGGTGTAAGCAGCAGCTACTGTCCATAGACCCAAGCCAGATGACCTTGAGCTATTGCAATATAGATGGGAATATTGGGTTCAACTCGTACATTGCGACCATGCGAGTGTTGCCCAAGGAAGATGGGTGCAGCATTGAGTGGAAGTATGAGGTTGAGCCAATTGCGGGGTGGACACTGAAGGATTTGGACATGCTTATTGGCACCGACTTGCAAGAGATGGCTAGCAGAATGGAAGCTAGCCTTAATCAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Polyketide_cyc2

Protein Analysis

169

Amino Acids

18.98

Weight (kDa)

4.81

Isoelectric Point (pI)

54.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Polyketide_cyc2 PF10604 29 - 164 7.8e-17 Polyketide cyclase / dehydrase and lipid transport
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 455
AciI CCGC 2 cut(s) 34, 419
AcuI CTGAAG 1 cut(s) 452
AfaI GTAC 1 cut(s) 344
AfiI CCNNNNNNNGG 2 cut(s) 167, 419
AgsI TTSAA 1 cut(s) 337
AjnI CCWGG 1 cut(s) 184
AluBI AGCT 4 cut(s) 70, 271, 306, 491
AluI AGCT 4 cut(s) 70, 271, 306, 491
AlwNI CAGNNNCTG 1 cut(s) 274
Ama87I CYCGRG 1 cut(s) 230
AoxI GGCC 2 cut(s) 98, 121
ApeKI GCWGC 5 cut(s) 181, 189, 265, 268, 384
AspLEI GCGC 3 cut(s) 198, 206, 250
AsuC2I CCSGG 1 cut(s) 178
AsuNHI GCTAGC 2 cut(s) 476, 491
AvaI CYCGRG 1 cut(s) 230
BanI GGYRCC 1 cut(s) 455
BbvI GCAGC 5 cut(s) 176, 193, 277, 280, 396
BccI CCATC 3 cut(s) 314, 371, 466
BciT130I CCWGG 1 cut(s) 186
BciVI GTATCC 1 cut(s) 184
BcnI CCSGG 1 cut(s) 178
BfaI CTAG 2 cut(s) 477, 492
BfuI GTATCC 1 cut(s) 184
BisI GCNGC 6 cut(s) 35, 182, 190, 266, 269, 385
BlsI GCNGC 6 cut(s) 36, 183, 191, 267, 270, 386
Bme1390I CCNGG 2 cut(s) 178, 186
BmeT110I CYCGRG 1 cut(s) 230
BmiI GGNNCC 2 cut(s) 6, 457
BmrFI CCNGG 2 cut(s) 178, 186
BmtI GCTAGC 2 cut(s) 480, 495
BpmI CTGGAG 3 cut(s) 128, 204, 228
BpuEI CTTGAG 1 cut(s) 322
BpuMI CCSGG 1 cut(s) 178
BsaJI CCNNGG 1 cut(s) 369
Bsc4I CCNNNNNNNGG 2 cut(s) 167, 419
Bse118I RCCGGY 1 cut(s) 244
Bse1I ACTGG 2 cut(s) 221, 257
Bse3DI GCAATG 1 cut(s) 345
BseBI CCWGG 1 cut(s) 186
BseDI CCNNGG 1 cut(s) 369
BseLI CCNNNNNNNGG 2 cut(s) 167, 419
BseMI GCAATG 1 cut(s) 345
BseNI ACTGG 2 cut(s) 221, 257
BseXI GCAGC 5 cut(s) 176, 193, 277, 280, 396
BsgI GTGCAG 2 cut(s) 84, 403
BshFI GGCC 2 cut(s) 100, 123
BshNI GGYRCC 1 cut(s) 455
BsiHKCI CYCGRG 1 cut(s) 230
BsiSI CCGG 2 cut(s) 177, 245
BslI CCNNNNNNNGG 2 cut(s) 167, 419
BsnI GGCC 2 cut(s) 100, 123
BsoBI CYCGRG 1 cut(s) 230
Bsp143I GATC 1 cut(s) 226
BspACI CCGC 2 cut(s) 34, 419
BspANI GGCC 2 cut(s) 100, 123
BspHI TCATGA 1 cut(s) 25
BspLI GGNNCC 2 cut(s) 6, 457
BspOI GCTAGC 2 cut(s) 480, 495
BspT107I GGYRCC 1 cut(s) 455
BsrDI GCAATG 1 cut(s) 345
BsrFI RCCGGY 1 cut(s) 244
BsrI ACTGG 2 cut(s) 221, 257
BssAI RCCGGY 1 cut(s) 244
BssECI CCNNGG 1 cut(s) 369
BssMI GATC 1 cut(s) 226
BssT1I CCWWGG 1 cut(s) 369
Bst2UI CCWGG 1 cut(s) 186
Bst4CI ACNGT 1 cut(s) 276
BstC8I GCNNGC 5 cut(s) 141, 194, 246, 478, 493
BstENI CCTNNNNNAGG 1 cut(s) 165
BstHHI GCGC 3 cut(s) 198, 206, 250
BstKTI GATC 1 cut(s) 229
BstMBI GATC 1 cut(s) 226
BstMWI GCNNNNNNNGC 2 cut(s) 195, 364
BstNI CCWGG 1 cut(s) 186
BstNSI RCATGY 2 cut(s) 156, 448
BstSCI CCNGG 2 cut(s) 176, 184
BstV1I GCAGC 5 cut(s) 176, 193, 277, 280, 396
BsuI GTATCC 1 cut(s) 184
BsuRI GGCC 2 cut(s) 100, 123
BtsIMutI CAGTG 2 cut(s) 250, 428
Cac8I GCNNGC 5 cut(s) 141, 194, 246, 478, 493
CaiI CAGNNNCTG 1 cut(s) 274
CciI TCATGA 1 cut(s) 25
CfoI GCGC 3 cut(s) 198, 206, 250
Cfr10I RCCGGY 1 cut(s) 244
Csp6I GTAC 1 cut(s) 343
CviAII CATG 4 cut(s) 26, 153, 355, 445
CviQI GTAC 1 cut(s) 343
DpnI GATC 1 cut(s) 228
DpnII GATC 1 cut(s) 226
Eco130I CCWWGG 1 cut(s) 369
Eco57I CTGAAG 1 cut(s) 452
Eco88I CYCGRG 1 cut(s) 230
EcoNI CCTNNNNNAGG 1 cut(s) 165
EcoRII CCWGG 1 cut(s) 184
EcoT14I CCWWGG 1 cut(s) 369
ErhI CCWWGG 1 cut(s) 369
FaeI CATG 4 cut(s) 29, 156, 358, 448
FaiI YATR 7 cut(s) 27, 154, 281, 317, 356, 402, 446
FalI AAGNNNNNCTT 1 cut(s) 480
FatI CATG 4 cut(s) 25, 152, 354, 444
FauI CCCGC 1 cut(s) 412
Fnu4HI GCNGC 6 cut(s) 35, 182, 190, 266, 269, 385
Fsp4HI GCNGC 6 cut(s) 35, 182, 190, 266, 269, 385
FspBI CTAG 2 cut(s) 477, 492
GlaI GCGC 3 cut(s) 197, 205, 249
GluI GCNGC 6 cut(s) 35, 182, 190, 266, 269, 385
GsuI CTGGAG 3 cut(s) 128, 204, 228
HaeIII GGCC 2 cut(s) 100, 123
HapII CCGG 2 cut(s) 177, 245
HhaI GCGC 3 cut(s) 198, 206, 250
Hin1II CATG 4 cut(s) 29, 156, 358, 448
Hin6I GCGC 3 cut(s) 196, 204, 248
HinP1I GCGC 3 cut(s) 196, 204, 248
HinfI GANTC 2 cut(s) 210, 217
HpaII CCGG 2 cut(s) 177, 245
Hpy166II GTNNAC 1 cut(s) 426
Hpy188III TCNNGA 4 cut(s) 26, 214, 230, 232
Hpy8I GTNNAC 1 cut(s) 426
HpyAV CCTTC 3 cut(s) 44, 167, 427
HpyCH4III ACNGT 1 cut(s) 276
HpyCH4V TGCA 5 cut(s) 65, 166, 312, 384, 466
HpyF10VI GCNNNNNNNGC 2 cut(s) 195, 364
Hsp92II CATG 4 cut(s) 29, 156, 358, 448
HspAI GCGC 3 cut(s) 196, 204, 248
KroI GCCGGC 1 cut(s) 244
KroNI GCCGGC 1 cut(s) 246
Kzo9I GATC 1 cut(s) 226
Lsp1109I GCAGC 5 cut(s) 176, 193, 277, 280, 396
MaeI CTAG 2 cut(s) 477, 492
MaeIII GTNAC 1 cut(s) 236
MalI GATC 1 cut(s) 228
MboI GATC 1 cut(s) 226
MboII GAAGA 1 cut(s) 386
MfeI CAATTG 1 cut(s) 414
MluCI AATT 1 cut(s) 414
MlyI GAGTC 2 cut(s) 211, 219
MnlI CCTC 4 cut(s) 33, 103, 155, 397
MroNI GCCGGC 1 cut(s) 244
MseI TTAA 1 cut(s) 498
MslI CAYNNNNRTG 1 cut(s) 359
MspA1I CMGCKG 1 cut(s) 34
MspI CCGG 2 cut(s) 177, 245
MspR9I CCNGG 2 cut(s) 178, 186
MunI CAATTG 1 cut(s) 414
MvaI CCWGG 1 cut(s) 186
MwoI GCNNNNNNNGC 2 cut(s) 195, 364
NaeI GCCGGC 1 cut(s) 246
NciI CCSGG 1 cut(s) 178
NdeII GATC 1 cut(s) 226
NgoMIV GCCGGC 1 cut(s) 244
NheI GCTAGC 2 cut(s) 476, 491
NlaIII CATG 4 cut(s) 29, 156, 358, 448
NlaIV GGNNCC 2 cut(s) 6, 457
NmuCI GTSAC 1 cut(s) 236
NspI RCATGY 2 cut(s) 156, 448
PaeR7I CTCGAG 1 cut(s) 230
PagI TCATGA 1 cut(s) 25
PcsI WCGNNNNNNNCGW 1 cut(s) 347
PdiI GCCGGC 1 cut(s) 246
PkrI GCNGC 6 cut(s) 36, 183, 191, 267, 270, 386
PleI GAGTC 2 cut(s) 211, 218
PpsI GAGTC 2 cut(s) 211, 218
Psp6I CCWGG 1 cut(s) 184
PspGI CCWGG 1 cut(s) 184
PspN4I GGNNCC 2 cut(s) 6, 457
PstNI CAGNNNCTG 1 cut(s) 274
RsaI GTAC 1 cut(s) 344
RsaNI GTAC 1 cut(s) 343
RseI CAYNNNNRTG 1 cut(s) 359
SaqAI TTAA 1 cut(s) 498
SatI GCNGC 6 cut(s) 35, 182, 190, 266, 269, 385
Sau3AI GATC 1 cut(s) 226
SchI GAGTC 2 cut(s) 211, 219
ScrFI CCNGG 2 cut(s) 178, 186
SetI ASST 7 cut(s) 10, 72, 273, 302, 308, 408, 493
Sfr274I CTCGAG 1 cut(s) 230
SlaI CTCGAG 1 cut(s) 230
SmiMI CAYNNNNRTG 1 cut(s) 359
SmlI CTYRAG 2 cut(s) 230, 301
SmoI CTYRAG 2 cut(s) 230, 301
Sse9I AATT 1 cut(s) 414
SsiI CCGC 2 cut(s) 34, 419
SspI AATATT 1 cut(s) 328
SspMI CTAG 2 cut(s) 477, 492
StyD4I CCNGG 2 cut(s) 176, 184
StyI CCWWGG 1 cut(s) 369
TaaI ACNGT 1 cut(s) 276
TaqI TCGA 1 cut(s) 231
TasI AATT 1 cut(s) 414
TauI GCSGC 1 cut(s) 37
Tru1I TTAA 1 cut(s) 498
Tru9I TTAA 1 cut(s) 498
TscAI CASTG 2 cut(s) 257, 435
TseFI GTSAC 1 cut(s) 236
TseI GCWGC 5 cut(s) 181, 189, 265, 268, 384
Tsp45I GTSAC 1 cut(s) 236
TspRI CASTG 2 cut(s) 257, 435
XagI CCTNNNNNAGG 1 cut(s) 165
XceI RCATGY 2 cut(s) 156, 448
XcmI CCANNNNNNNNNTGG 1 cut(s) 420
XhoI CTCGAG 1 cut(s) 230
XspI CTAG 2 cut(s) 477, 492
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.