MD08G1112100.v1.1

Polyketide cyclase / dehydrase and lipid transport

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0006645
Physical Location & Seq
Forward (+)
9904787 .. 9905281
495 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1112100.v1.1.491

Sequence Viewer

Length: 495 bp
ATGGAGCAGCACTCAGATCCAAAGTGGGAAGCCAAGGTTTCTGCAACCCTAAAAGATGCCACAGTGGACCAGATATGGCCTCTCTTCAAAGACTTCTTTAATTTCCACAGGTGGTTTCCAACCCTATCCACTTGCCATGGGATTCACGGAACCAACGGTGAACCCGGCTGTGTCAGATATTGCTCGGGGTTCTCAATCCCCTCCAAAAGTGGCGAGAAGTCCGTCAGCTGGTCCAAGGAACGATTAATAGCCGTTGATGATGCTGACCATAGTCTGAGCTACGAGATCGTGGAGAGCAACCTTGGGTTCAATTCGTACGTCTCGACGTTCAGGATTGTTCCGCGTGGGGATATTGATGGTCGAGATGGGTGCGTGATCGAGTGGTCCATAACCGTTGATCCTGTGGAAGGGTGGGTTTTCGGTGATCTGGTGAGGAAGTATGAGTCTGGGTTGCAGAAAATGTCTAATAGAATAGAAGATGCAATTTGTATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Polyketide_cyc2

Protein Analysis

165

Amino Acids

18.53

Weight (kDa)

5.17

Isoelectric Point (pI)

23.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Polyketide_cyc2 PF10604 10 - 150 1.4e-23 Polyketide cyclase / dehydrase and lipid transport
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 343
AciI CCGC 1 cut(s) 341
AclWI GGATC 2 cut(s) 11, 392
AfaI GTAC 1 cut(s) 317
AfiI CCNNNNNNNGG 2 cut(s) 228, 407
AgsI TTSAA 2 cut(s) 88, 310
AluBI AGCT 2 cut(s) 228, 279
AluI AGCT 2 cut(s) 228, 279
Alw26I GTCTC 1 cut(s) 325
AlwI GGATC 2 cut(s) 11, 392
Ama87I CYCGRG 1 cut(s) 184
AoxI GGCC 1 cut(s) 77
ApeKI GCWGC 1 cut(s) 7
AseI ATTAAT 1 cut(s) 245
AspS9I GGNCC 3 cut(s) 67, 231, 384
AsuC2I CCSGG 1 cut(s) 165
AsuHPI GGTGA 3 cut(s) 170, 434, 442
AvaI CYCGRG 1 cut(s) 184
AvaII GGWCC 3 cut(s) 67, 231, 384
BbvI GCAGC 1 cut(s) 19
BccI CCATC 2 cut(s) 350, 359
BceAI ACGGC 1 cut(s) 236
BcgI CGANNNNNNTGC 2 cut(s) 351, 385
BcnI CCSGG 1 cut(s) 165
BcoDI GTCTC 1 cut(s) 325
BisI GCNGC 1 cut(s) 8
BlsI GCNGC 1 cut(s) 9
Bme1390I CCNGG 1 cut(s) 165
Bme18I GGWCC 3 cut(s) 67, 231, 384
BmeT110I CYCGRG 1 cut(s) 184
BmgT120I GGNCC 3 cut(s) 67, 231, 384
BmiI GGNNCC 1 cut(s) 151
BmrFI CCNGG 1 cut(s) 165
BmsI GCATC 3 cut(s) 46, 250, 469
BoxI GACNNNNGTC 1 cut(s) 270
BplI GAGNNNNNCTC 1 cut(s) 28
BpuMI CCSGG 1 cut(s) 165
BsaJI CCNNGG 4 cut(s) 33, 136, 234, 301
Bsc4I CCNNNNNNNGG 2 cut(s) 228, 407
BseDI CCNNGG 4 cut(s) 33, 136, 234, 301
BseLI CCNNNNNNNGG 2 cut(s) 228, 407
BseMII CTCAG 2 cut(s) 27, 266
BseXI GCAGC 1 cut(s) 19
Bsh1236I CGCG 1 cut(s) 343
BshFI GGCC 1 cut(s) 79
BsiHKCI CYCGRG 1 cut(s) 184
BsiSI CCGG 1 cut(s) 165
BsiWI CGTACG 1 cut(s) 315
BslI CCNNNNNNNGG 2 cut(s) 228, 407
BsmAI GTCTC 1 cut(s) 325
BsmBI CGTCTC 1 cut(s) 325
BsnI GGCC 1 cut(s) 79
BsoBI CYCGRG 1 cut(s) 184
Bsp143I GATC 5 cut(s) 16, 285, 375, 397, 424
Bsp19I CCATGG 1 cut(s) 136
BspACI CCGC 1 cut(s) 341
BspANI GGCC 1 cut(s) 79
BspCNI CTCAG 2 cut(s) 26, 267
BspFNI CGCG 1 cut(s) 343
BspLI GGNNCC 1 cut(s) 151
BspPI GGATC 2 cut(s) 11, 392
BssECI CCNNGG 4 cut(s) 33, 136, 234, 301
BssMI GATC 5 cut(s) 16, 285, 375, 397, 424
BssT1I CCWWGG 4 cut(s) 33, 136, 234, 301
Bst4CI ACNGT 3 cut(s) 64, 158, 394
Bst6I CTCTTC 1 cut(s) 89
BstDEI CTNAG 2 cut(s) 13, 275
BstDSI CCRYGG 1 cut(s) 136
BstENI CCTNNNNNAGG 1 cut(s) 405
BstFNI CGCG 1 cut(s) 343
BstKTI GATC 5 cut(s) 19, 288, 378, 400, 427
BstMAI GTCTC 1 cut(s) 325
BstMBI GATC 5 cut(s) 16, 285, 375, 397, 424
BstPAI GACNNNNGTC 1 cut(s) 270
BstSCI CCNGG 1 cut(s) 163
BstUI CGCG 1 cut(s) 343
BstV1I GCAGC 1 cut(s) 19
BstX2I RGATCY 1 cut(s) 16
BstYI RGATCY 1 cut(s) 16
BsuRI GGCC 1 cut(s) 79
BtgI CCRYGG 1 cut(s) 136
BtsIMutI CAGTG 1 cut(s) 69
Cfr13I GGNCC 3 cut(s) 67, 231, 384
Csp6I GTAC 1 cut(s) 316
CviAII CATG 1 cut(s) 137
CviJI RGCY 6 cut(s) 32, 79, 168, 228, 251, 279
CviKI_1 RGCY 6 cut(s) 32, 79, 168, 228, 251, 279
CviQI GTAC 1 cut(s) 316
DdeI CTNAG 2 cut(s) 13, 275
DpnI GATC 5 cut(s) 18, 287, 377, 399, 426
DpnII GATC 5 cut(s) 16, 285, 375, 397, 424
Eam1104I CTCTTC 1 cut(s) 89
EarI CTCTTC 1 cut(s) 89
Eco130I CCWWGG 4 cut(s) 33, 136, 234, 301
Eco47I GGWCC 3 cut(s) 67, 231, 384
Eco88I CYCGRG 1 cut(s) 184
EcoNI CCTNNNNNAGG 1 cut(s) 405
EcoT14I CCWWGG 4 cut(s) 33, 136, 234, 301
ErhI CCWWGG 4 cut(s) 33, 136, 234, 301
Esp3I CGTCTC 1 cut(s) 325
FaeI CATG 1 cut(s) 140
FaiI YATR 5 cut(s) 76, 138, 270, 389, 441
FatI CATG 1 cut(s) 136
Fnu4HI GCNGC 1 cut(s) 8
Fsp4HI GCNGC 1 cut(s) 8
GluI GCNGC 1 cut(s) 8
HaeIII GGCC 1 cut(s) 79
HapII CCGG 1 cut(s) 165
Hin1II CATG 1 cut(s) 140
HinfI GANTC 2 cut(s) 142, 443
HpaII CCGG 1 cut(s) 165
HphI GGTGA 3 cut(s) 170, 434, 442
Hpy166II GTNNAC 2 cut(s) 67, 161
Hpy188I TCNGA 3 cut(s) 16, 176, 276
Hpy188III TCNNGA 3 cut(s) 322, 331, 362
Hpy8I GTNNAC 2 cut(s) 67, 161
Hpy99I CGWCG 1 cut(s) 328
HpyAV CCTTC 1 cut(s) 401
HpyCH4III ACNGT 3 cut(s) 64, 158, 394
HpyCH4IV ACGT 2 cut(s) 318, 326
HpyCH4V TGCA 3 cut(s) 44, 454, 482
HpyF3I CTNAG 2 cut(s) 13, 275
HpySE526I ACGT 2 cut(s) 318, 326
Hsp92II CATG 1 cut(s) 140
Kzo9I GATC 5 cut(s) 16, 285, 375, 397, 424
LmnI GCTCC 1 cut(s) 4
LpnPI CCDG 8 cut(s) 83, 94, 178, 214, 316, 413, 414, 432
Lsp1109I GCAGC 1 cut(s) 19
LweI GCATC 3 cut(s) 46, 250, 469
MaeII ACGT 2 cut(s) 318, 326
MalI GATC 5 cut(s) 18, 287, 377, 399, 426
MboI GATC 5 cut(s) 16, 285, 375, 397, 424
MboII GAAGA 2 cut(s) 76, 488
MflI RGATCY 1 cut(s) 16
MluCI AATT 3 cut(s) 100, 310, 483
MlyI GAGTC 1 cut(s) 452
MmeI TCCRAC 1 cut(s) 143
MnlI CCTC 3 cut(s) 90, 211, 426
MseI TTAA 2 cut(s) 99, 245
MspA1I CMGCKG 1 cut(s) 228
MspI CCGG 1 cut(s) 165
MspR9I CCNGG 1 cut(s) 165
MvnI CGCG 1 cut(s) 343
NciI CCSGG 1 cut(s) 165
NcoI CCATGG 1 cut(s) 136
NdeII GATC 5 cut(s) 16, 285, 375, 397, 424
NlaIII CATG 1 cut(s) 140
NlaIV GGNNCC 1 cut(s) 151
PcsI WCGNNNNNNNCGW 1 cut(s) 320
PfeI GAWTC 1 cut(s) 142
Pfl23II CGTACG 1 cut(s) 315
PkrI GCNGC 1 cut(s) 9
PleI GAGTC 1 cut(s) 451
PpsI GAGTC 1 cut(s) 451
PshAI GACNNNNGTC 1 cut(s) 270
PshBI ATTAAT 1 cut(s) 245
PspLI CGTACG 1 cut(s) 315
PspN4I GGNNCC 1 cut(s) 151
PspPI GGNCC 3 cut(s) 67, 231, 384
PsuI RGATCY 1 cut(s) 16
PvuII CAGCTG 1 cut(s) 228
RsaI GTAC 1 cut(s) 317
RsaNI GTAC 1 cut(s) 316
SaqAI TTAA 2 cut(s) 99, 245
SatI GCNGC 1 cut(s) 8
Sau3AI GATC 5 cut(s) 16, 285, 375, 397, 424
Sau96I GGNCC 3 cut(s) 67, 231, 384
SchI GAGTC 1 cut(s) 452
ScrFI CCNGG 1 cut(s) 165
SetI ASST 7 cut(s) 39, 113, 230, 281, 303, 321, 329
SfaNI GCATC 3 cut(s) 46, 250, 469
SinI GGWCC 3 cut(s) 67, 231, 384
Sse9I AATT 3 cut(s) 100, 310, 483
SsiI CCGC 1 cut(s) 341
StyD4I CCNGG 1 cut(s) 163
StyI CCWWGG 4 cut(s) 33, 136, 234, 301
TaaI ACNGT 3 cut(s) 64, 158, 394
TaiI ACGT 2 cut(s) 321, 329
TaqI TCGA 3 cut(s) 323, 361, 378
TasI AATT 3 cut(s) 100, 310, 483
TfiI GAWTC 1 cut(s) 142
Tru1I TTAA 2 cut(s) 99, 245
Tru9I TTAA 2 cut(s) 99, 245
TscAI CASTG 1 cut(s) 69
TseI GCWGC 1 cut(s) 7
TspGWI ACGGA 2 cut(s) 162, 211
TspRI CASTG 1 cut(s) 69
VpaK11BI GGWCC 3 cut(s) 67, 231, 384
VspI ATTAAT 1 cut(s) 245
XagI CCTNNNNNAGG 1 cut(s) 405
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.