AT2G27035

Plastocyanin-like domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0005650
Physical Location & Seq
Forward (+)
11535518 .. 11536485
968 bp
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UTR
Exon/CDS
Intron
AT2G27035.1

Sequence Viewer

Length: 492 bp
ATGATGGGGAAATATTTATGGGCGTTAGTATATGTAACGGTGATGATATTAATTATAGTAGTTGAAGTGGAATCAAGTCTTCATAGAGTAGGCGGAGGAAGATACACTTGGAACTCCGATGTCAACTTCTCCGATTGGGCCAACCATCAACGTTTCTACTCCGGCGACTGGCTTTATTTCGGGTTCAATCGAACGCGTCACAATATCCTTCAAGTTAACAAGAGTAGCTACGAGCAATGCGTTGACAACGATTACATATTCAACATAACACGAGGAGGACGAGATGTTTTTCAACTCCTTGAACCAAAGCCTTATTATTTCATTTGTGGTAGAGGTTATTGTCTCAAAGGTATGAAGCTTGCAATCACTGTCCTTCCTCAACCGCCACCATCAGCTCCAACCAATTTCACCTCAACCACCACTCCTCTCATACCACCAAATGCTATCACTGCAGCCATTCTCATTTTCGCCTTTAAAGCTTTACTACTTTGA

Protein Analysis

163

Amino Acids

18.61

Weight (kDa)

9.01

Isoelectric Point (pI)

32.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu_bind_like PF02298 37 - 118 9.9e-18 Plastocyanin-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 196
AciI CCGC 2 cut(s) 93, 383
AclI AACGTT 1 cut(s) 151
AfiI CCNNNNNNNGG 1 cut(s) 168
AflIII ACRYGT 1 cut(s) 194
AgsI TTSAA 6 cut(s) 65, 187, 212, 262, 293, 302
AjuI GAANNNNNNNTTGG 2 cut(s) 91, 123
AluBI AGCT 4 cut(s) 228, 358, 395, 479
AluI AGCT 4 cut(s) 228, 358, 395, 479
Alw26I GTCTC 1 cut(s) 347
AoxI GGCC 1 cut(s) 138
ApeKI GCWGC 1 cut(s) 452
AseI ATTAAT 1 cut(s) 50
AspS9I GGNCC 1 cut(s) 138
AsuHPI GGTGA 2 cut(s) 52, 400
BauI CACGAG 1 cut(s) 270
BbsI GAAGAC 1 cut(s) 71
BbvI GCAGC 1 cut(s) 464
BccI CCATC 2 cut(s) 153, 397
BcoDI GTCTC 1 cut(s) 347
BfmI CTRYAG 1 cut(s) 450
BisI GCNGC 1 cut(s) 453
BlsI GCNGC 1 cut(s) 454
BmgT120I GGNCC 1 cut(s) 138
BpiI GAAGAC 1 cut(s) 71
BsaXI ACNNNNNCTCC 2 cut(s) 406, 436
Bsc4I CCNNNNNNNGG 1 cut(s) 168
Bse1I ACTGG 1 cut(s) 173
Bse3DI GCAATG 1 cut(s) 242
BseLI CCNNNNNNNGG 1 cut(s) 168
BseMI GCAATG 1 cut(s) 242
BseNI ACTGG 1 cut(s) 173
BseRI GAGGAG 2 cut(s) 288, 414
BseXI GCAGC 1 cut(s) 464
Bsh1236I CGCG 1 cut(s) 196
BshFI GGCC 1 cut(s) 140
BsiSI CCGG 1 cut(s) 162
BslI CCNNNNNNNGG 1 cut(s) 168
BsmAI GTCTC 1 cut(s) 347
BsnI GGCC 1 cut(s) 140
BspACI CCGC 2 cut(s) 93, 383
BspANI GGCC 1 cut(s) 140
BspFNI CGCG 1 cut(s) 196
BspMAI CTGCAG 1 cut(s) 454
BsrDI GCAATG 1 cut(s) 242
BsrI ACTGG 1 cut(s) 173
BssSI CACGAG 1 cut(s) 270
Bst2BI CACGAG 1 cut(s) 270
Bst4CI ACNGT 2 cut(s) 40, 370
BstC8I GCNNGC 1 cut(s) 360
BstFNI CGCG 1 cut(s) 196
BstMAI GTCTC 1 cut(s) 347
BstMWI GCNNNNNNNGC 2 cut(s) 449, 476
BstSFI CTRYAG 1 cut(s) 450
BstUI CGCG 1 cut(s) 196
BstV1I GCAGC 1 cut(s) 464
BstV2I GAAGAC 1 cut(s) 71
BsuRI GGCC 1 cut(s) 140
BtsI GCAGTG 1 cut(s) 447
BtsIMutI CAGTG 2 cut(s) 366, 447
Cac8I GCNNGC 1 cut(s) 360
Cfr13I GGNCC 1 cut(s) 138
CseI GACGC 1 cut(s) 185
CviJI RGCY 8 cut(s) 140, 172, 228, 310, 358, 395, 455, 479
CviKI_1 RGCY 8 cut(s) 140, 172, 228, 310, 358, 395, 455, 479
DraI TTTAAA 1 cut(s) 475
EciI GGCGGA 1 cut(s) 108
FaiI YATR 9 cut(s) 19, 31, 33, 56, 84, 257, 266, 353, 431
FalI AAGNNNNNCTT 2 cut(s) 91, 123
Fnu4HI GCNGC 1 cut(s) 453
Fsp4HI GCNGC 1 cut(s) 453
GluI GCNGC 1 cut(s) 453
HaeIII GGCC 1 cut(s) 140
HapII CCGG 1 cut(s) 162
HgaI GACGC 1 cut(s) 185
HincII GTYRAC 3 cut(s) 124, 217, 244
HindII GTYRAC 3 cut(s) 124, 217, 244
HindIII AAGCTT 2 cut(s) 356, 477
HinfI GANTC 1 cut(s) 71
HpaI GTTAAC 1 cut(s) 217
HpaII CCGG 1 cut(s) 162
HphI GGTGA 2 cut(s) 52, 400
Hpy166II GTNNAC 3 cut(s) 124, 217, 244
Hpy188I TCNGA 2 cut(s) 118, 133
Hpy8I GTNNAC 3 cut(s) 124, 217, 244
HpyAV CCTTC 2 cut(s) 218, 383
HpyCH4III ACNGT 2 cut(s) 40, 370
HpyCH4IV ACGT 1 cut(s) 151
HpyCH4V TGCA 2 cut(s) 362, 452
HpyF10VI GCNNNNNNNGC 2 cut(s) 449, 476
HpySE526I ACGT 1 cut(s) 151
KspAI GTTAAC 1 cut(s) 217
LmnI GCTCC 1 cut(s) 400
LpnPI CCDG 2 cut(s) 154, 175
Lsp1109I GCAGC 1 cut(s) 464
MaeII ACGT 1 cut(s) 151
MaeIII GTNAC 2 cut(s) 34, 197
MboII GAAGA 2 cut(s) 71, 111
MluCI AATT 2 cut(s) 51, 403
MluI ACGCGT 1 cut(s) 194
MmeI TCCRAC 1 cut(s) 422
MnlI CCTC 7 cut(s) 89, 266, 269, 326, 387, 421, 435
MseI TTAA 3 cut(s) 50, 216, 474
MspI CCGG 1 cut(s) 162
MvnI CGCG 1 cut(s) 196
MwoI GCNNNNNNNGC 2 cut(s) 449, 476
NmuCI GTSAC 1 cut(s) 197
PcsI WCGNNNNNNNCGW 2 cut(s) 237, 277
PfeI GAWTC 1 cut(s) 71
PkrI GCNGC 1 cut(s) 454
PshBI ATTAAT 1 cut(s) 50
Psp1406I AACGTT 1 cut(s) 151
PspPI GGNCC 1 cut(s) 138
PstI CTGCAG 1 cut(s) 454
SaqAI TTAA 3 cut(s) 50, 216, 474
SatI GCNGC 1 cut(s) 453
Sau96I GGNCC 1 cut(s) 138
SetI ASST 8 cut(s) 154, 230, 337, 352, 360, 397, 413, 481
SfcI CTRYAG 1 cut(s) 450
Sse9I AATT 2 cut(s) 51, 403
SsiI CCGC 2 cut(s) 93, 383
SspI AATATT 1 cut(s) 14
TaaI ACNGT 2 cut(s) 40, 370
TaiI ACGT 1 cut(s) 154
TaqI TCGA 1 cut(s) 190
TasI AATT 2 cut(s) 51, 403
TfiI GAWTC 1 cut(s) 71
Tru1I TTAA 3 cut(s) 50, 216, 474
Tru9I TTAA 3 cut(s) 50, 216, 474
TscAI CASTG 2 cut(s) 373, 454
TseFI GTSAC 1 cut(s) 197
TseI GCWGC 1 cut(s) 452
Tsp45I GTSAC 1 cut(s) 197
TspDTI ATGAA 3 cut(s) 71, 310, 368
TspRI CASTG 2 cut(s) 373, 454
VspI ATTAAT 1 cut(s) 50
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.