FvH4_6g27830

Lamin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0005650
Physical Location & Seq
Reverse (-)
21433169 .. 21434106
938 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g27830.t1

Sequence Viewer

Length: 537 bp
ATGAAGCAAAAGCCAACAAGTAATCCACAGATAGGTAGTGATATTGAGCTAGCTAAGGTTGAGAGTGGAGGGGAACCAGTGCTTCATAGGGTTGGAGGGGGAAGGATGACTTGGATCCCCGATATTAACTTCACTGATTGGTCAAGTAAAGAGCATTTCTATGCAGGAGATTGGCTTTACTTTGGGTTTGACAAACAGCTCTACAATGTCCTAGAGGTAAACAAGACCAGTTGCGAGAGTTGCATCGACAAAGATTACATATACAATGTCACACGAGGCGGGAGTGATGTGTTCAACCTGACAGAGGCAAAGACATATTACTTTCTGAGCGGCCGAGGCTATTGCTACAAAGGGATGAAAGTAGCTGTCTTTGTTGAGCAATCCCCTCCTGAACTTCACCGCAACCATGGGTTTGCAGGGAGTACTAAATTCCCATTTGATGCCAGTAAGCAACCACCAAGGAAGAGCAGAGAGGAAGCCAAGTTTAAATCGAAGTTGATGGACTTGGTGATGTTCTCTTTCATCTGGGGAAGCTAA

Protein Analysis

179

Amino Acids

20.31

Weight (kDa)

8.33

Isoelectric Point (pI)

41.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu_bind_like PF02298 40 - 119 3.5e-16 Plastocyanin-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 330
AciI CCGC 3 cut(s) 279, 330, 400
AclWI GGATC 2 cut(s) 109, 122
AcoI YGGCCR 1 cut(s) 331
AcsI RAATTY 1 cut(s) 428
AfaI GTAC 1 cut(s) 424
AfiI CCNNNNNNNGG 2 cut(s) 32, 304
AgsI TTSAA 1 cut(s) 295
AjuI GAANNNNNNNTTGG 2 cut(s) 94, 126
AluBI AGCT 5 cut(s) 49, 53, 199, 365, 534
AluI AGCT 5 cut(s) 49, 53, 199, 365, 534
AlwI GGATC 2 cut(s) 109, 122
AoxI GGCC 1 cut(s) 331
ApoI RAATTY 1 cut(s) 428
AsuHPI GGTGA 2 cut(s) 389, 520
AsuNHI GCTAGC 1 cut(s) 49
BamHI GGATCC 1 cut(s) 114
BauI CACGAG 1 cut(s) 273
BccI CCATC 1 cut(s) 493
BcgI CGANNNNNNTGC 2 cut(s) 324, 358
BfaI CTAG 2 cut(s) 50, 212
BisI GCNGC 1 cut(s) 331
BlsI GCNGC 1 cut(s) 332
BmcAI AGTACT 1 cut(s) 424
BmiI GGNNCC 2 cut(s) 75, 116
BmsI GCATC 2 cut(s) 252, 430
BmtI GCTAGC 1 cut(s) 53
Bpu10I CCTNAGC 1 cut(s) 54
BsaJI CCNNGG 3 cut(s) 334, 406, 458
Bsc4I CCNNNNNNNGG 2 cut(s) 32, 304
Bse1I ACTGG 3 cut(s) 77, 228, 444
BseDI CCNNGG 3 cut(s) 334, 406, 458
BseGI GGATG 2 cut(s) 111, 360
BseLI CCNNNNNNNGG 2 cut(s) 32, 304
BseMII CTCAG 1 cut(s) 317
BseNI ACTGG 3 cut(s) 77, 228, 444
BseX3I CGGCCG 1 cut(s) 331
Bsh1285I CGRYCG 1 cut(s) 334
BshFI GGCC 1 cut(s) 333
BsiEI CGRYCG 1 cut(s) 334
BslI CCNNNNNNNGG 2 cut(s) 32, 304
BsnI GGCC 1 cut(s) 333
Bsp143I GATC 1 cut(s) 114
Bsp19I CCATGG 1 cut(s) 406
BspACI CCGC 3 cut(s) 279, 330, 400
BspANI GGCC 1 cut(s) 333
BspCNI CTCAG 1 cut(s) 318
BspLI GGNNCC 2 cut(s) 75, 116
BspOI GCTAGC 1 cut(s) 53
BspPI GGATC 2 cut(s) 109, 122
BspQI GCTCTTC 1 cut(s) 458
BsrBI CCGCTC 1 cut(s) 330
BsrI ACTGG 3 cut(s) 77, 228, 444
BssECI CCNNGG 3 cut(s) 334, 406, 458
BssMI GATC 1 cut(s) 114
BssSI CACGAG 1 cut(s) 273
BssT1I CCWWGG 2 cut(s) 406, 458
Bst2BI CACGAG 1 cut(s) 273
Bst6I CTCTTC 1 cut(s) 458
BstC8I GCNNGC 1 cut(s) 51
BstDEI CTNAG 2 cut(s) 54, 326
BstDSI CCRYGG 1 cut(s) 406
BstENI CCTNNNNNAGG 1 cut(s) 302
BstF5I GGATG 2 cut(s) 111, 360
BstKTI GATC 1 cut(s) 117
BstMBI GATC 1 cut(s) 114
BstMCI CGRYCG 1 cut(s) 334
BstMWI GCNNNNNNNGC 2 cut(s) 240, 336
BstX2I RGATCY 1 cut(s) 114
BstYI RGATCY 1 cut(s) 114
BstZI CGGCCG 1 cut(s) 331
BsuRI GGCC 1 cut(s) 333
BtgI CCRYGG 1 cut(s) 406
BtsCI GGATG 2 cut(s) 111, 360
BtsIMutI CAGTG 2 cut(s) 84, 132
Cac8I GCNNGC 1 cut(s) 51
Csp6I GTAC 1 cut(s) 423
CviAII CATG 1 cut(s) 407
CviQI GTAC 1 cut(s) 423
DdeI CTNAG 2 cut(s) 54, 326
DpnI GATC 1 cut(s) 116
DpnII GATC 1 cut(s) 114
DraI TTTAAA 1 cut(s) 487
EaeI YGGCCR 1 cut(s) 331
EagI CGGCCG 1 cut(s) 331
Eam1104I CTCTTC 1 cut(s) 458
EarI CTCTTC 1 cut(s) 458
EclXI CGGCCG 1 cut(s) 331
Eco130I CCWWGG 2 cut(s) 406, 458
Eco52I CGGCCG 1 cut(s) 331
EcoNI CCTNNNNNAGG 1 cut(s) 302
EcoT14I CCWWGG 2 cut(s) 406, 458
ErhI CCWWGG 2 cut(s) 406, 458
FaeI CATG 1 cut(s) 410
FaiI YATR 6 cut(s) 87, 162, 260, 262, 316, 408
FalI AAGNNNNNCTT 2 cut(s) 94, 126
FatI CATG 1 cut(s) 406
FauI CCCGC 1 cut(s) 272
Fnu4HI GCNGC 1 cut(s) 331
FokI GGATG 2 cut(s) 118, 367
Fsp4HI GCNGC 1 cut(s) 331
FspBI CTAG 2 cut(s) 50, 212
GluI GCNGC 1 cut(s) 331
HaeIII GGCC 1 cut(s) 333
Hin1II CATG 1 cut(s) 410
HphI GGTGA 2 cut(s) 389, 520
Hpy166II GTNNAC 1 cut(s) 220
Hpy188I TCNGA 1 cut(s) 327
Hpy188III TCNNGA 1 cut(s) 389
Hpy8I GTNNAC 1 cut(s) 220
HpyAV CCTTC 1 cut(s) 96
HpyCH4V TGCA 3 cut(s) 164, 243, 416
HpyF10VI GCNNNNNNNGC 2 cut(s) 240, 336
HpyF3I CTNAG 2 cut(s) 54, 326
Hsp92II CATG 1 cut(s) 410
Kzo9I GATC 1 cut(s) 114
LguI GCTCTTC 1 cut(s) 458
LpnPI CCDG 8 cut(s) 90, 150, 241, 311, 402, 402, 457, 511
LweI GCATC 2 cut(s) 252, 430
MaeI CTAG 2 cut(s) 50, 212
MaeIII GTNAC 1 cut(s) 268
MalI GATC 1 cut(s) 116
MbiI CCGCTC 1 cut(s) 330
MboI GATC 1 cut(s) 114
MboII GAAGA 1 cut(s) 475
MflI RGATCY 1 cut(s) 114
MluCI AATT 1 cut(s) 428
MmeI TCCRAC 1 cut(s) 73
MnlI CCTC 8 cut(s) 62, 89, 208, 269, 298, 329, 396, 466
MseI TTAA 2 cut(s) 126, 486
MslI CAYNNNNRTG 1 cut(s) 159
MwoI GCNNNNNNNGC 2 cut(s) 240, 336
NcoI CCATGG 1 cut(s) 406
NdeII GATC 1 cut(s) 114
NheI GCTAGC 1 cut(s) 49
NlaIII CATG 1 cut(s) 410
NlaIV GGNNCC 2 cut(s) 75, 116
NmeAIII GCCGAG 1 cut(s) 359
NmuCI GTSAC 1 cut(s) 268
PciSI GCTCTTC 1 cut(s) 458
PkrI GCNGC 1 cut(s) 332
PspN4I GGNNCC 2 cut(s) 75, 116
PsuI RGATCY 1 cut(s) 114
RsaI GTAC 1 cut(s) 424
RsaNI GTAC 1 cut(s) 423
RseI CAYNNNNRTG 1 cut(s) 159
SapI GCTCTTC 1 cut(s) 458
SaqAI TTAA 2 cut(s) 126, 486
SatI GCNGC 1 cut(s) 331
Sau3AI GATC 1 cut(s) 114
ScaI AGTACT 1 cut(s) 424
SetI ASST 9 cut(s) 37, 51, 55, 60, 201, 219, 300, 367, 536
SfaNI GCATC 2 cut(s) 252, 430
SmiMI CAYNNNNRTG 1 cut(s) 159
Sse9I AATT 1 cut(s) 428
SsiI CCGC 3 cut(s) 279, 330, 400
SspMI CTAG 2 cut(s) 50, 212
StyI CCWWGG 2 cut(s) 406, 458
TaqI TCGA 2 cut(s) 246, 491
TasI AATT 1 cut(s) 428
TatI WGTACW 1 cut(s) 422
TauI GCSGC 1 cut(s) 333
Tru1I TTAA 2 cut(s) 126, 486
Tru9I TTAA 2 cut(s) 126, 486
TscAI CASTG 2 cut(s) 84, 139
TseFI GTSAC 1 cut(s) 268
Tsp45I GTSAC 1 cut(s) 268
TspDTI ATGAA 4 cut(s) 17, 74, 371, 511
TspRI CASTG 2 cut(s) 84, 139
XagI CCTNNNNNAGG 1 cut(s) 302
XapI RAATTY 1 cut(s) 428
XspI CTAG 2 cut(s) 50, 212
ZrmI AGTACT 1 cut(s) 424
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.