Rroxscaffold_1G00031480

Lamin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0005650
Physical Location & Seq
Reverse (-)
44410411 .. 44411099
689 bp
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UTR
Exon/CDS
Intron
Rroxscaffold_1G00031480.1

Sequence Viewer

Length: 528 bp
ATGCATGTGCTGCCACTTCTTAAGTGCCATGGCCTCAATGGAGCACAGCCAACAAGTAATCCACAGATAGATGGAGAGATTGAGCTATCTAGGGTTGAGAATGGAGGGGAGCCAGTCCTTCATAGGGTTGGAGGGGGAAGGTTCAGTTGGGCCCCCGATATTAACTTCACAGATTGGTCAAGTAAAGAGCATTTCTATGTAGGCGATTGGCTCTATTTTGGGTTTGACAAACATATCTACAATGTCCTTGAGGTAAACAAGACCAGTTACGAGAATTGCTTCGACAAAGAATACATATACAATGTTACACGAGGCGGGCGTGATGTGTTCAACTTGACAGAGGCAAAGACATACTACTTTCTGAGCGGCCGAGGCTATTGCTACAAAGGGATGAAAGTTGCTGTTTTTGTTGAGCAAACGCCACCCGTACTTCACCTCAGCCCTGGTTTCAAATCATATTCATATACCAGCATCAACTTTATCATCATTCTCACTATGCTTGCCACTTGGACAATCATTTCTTACTAG

Protein Analysis

175

Amino Acids

20.06

Weight (kDa)

6.29

Isoelectric Point (pI)

30.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu_bind_like PF02298 50 - 131 3.5e-18 Plastocyanin-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 366
AciI CCGC 2 cut(s) 315, 366
AcoI YGGCCR 1 cut(s) 367
AfaI GTAC 1 cut(s) 429
AfiI CCNNNNNNNGG 1 cut(s) 124
AflII CTTAAG 1 cut(s) 20
AgsI TTSAA 2 cut(s) 331, 451
AjnI CCWGG 1 cut(s) 442
AjuI GAANNNNNNNTTGG 2 cut(s) 130, 162
AluBI AGCT 1 cut(s) 85
AluI AGCT 1 cut(s) 85
Alw21I GWGCWC 1 cut(s) 46
AoxI GGCC 3 cut(s) 31, 150, 367
ApaI GGGCCC 1 cut(s) 154
ApeKI GCWGC 1 cut(s) 10
Asp700I GAANNNNTTC 1 cut(s) 278
AspS9I GGNCC 2 cut(s) 150, 151
AsuHPI GGTGA 1 cut(s) 425
BaeGI GKGCMC 1 cut(s) 154
BanII GRGCYC 1 cut(s) 154
BauI CACGAG 1 cut(s) 309
Bbv12I GWGCWC 1 cut(s) 46
BbvCI CCTCAGC 1 cut(s) 437
BccI CCATC 1 cut(s) 65
BcgI CGANNNNNNTGC 2 cut(s) 360, 394
BciT130I CCWGG 1 cut(s) 444
BfaI CTAG 2 cut(s) 90, 526
BfrI CTTAAG 1 cut(s) 20
BisI GCNGC 2 cut(s) 11, 367
BlsI GCNGC 2 cut(s) 12, 368
Bme1390I CCNGG 1 cut(s) 444
BmgT120I GGNCC 2 cut(s) 150, 151
BmiI GGNNCC 3 cut(s) 111, 152, 153
BmrFI CCNGG 1 cut(s) 444
BmsI GCATC 1 cut(s) 480
Bpu10I CCTNAGC 1 cut(s) 437
BpuEI CTTGAG 1 cut(s) 269
BsaJI CCNNGG 3 cut(s) 28, 370, 442
Bsc4I CCNNNNNNNGG 1 cut(s) 124
Bse1I ACTGG 2 cut(s) 113, 264
BseBI CCWGG 1 cut(s) 444
BseDI CCNNGG 3 cut(s) 28, 370, 442
BseGI GGATG 1 cut(s) 396
BseLI CCNNNNNNNGG 1 cut(s) 124
BseMII CTCAG 2 cut(s) 353, 451
BseNI ACTGG 2 cut(s) 113, 264
BseSI GKGCMC 1 cut(s) 154
BseX3I CGGCCG 1 cut(s) 367
Bsh1285I CGRYCG 1 cut(s) 370
BshFI GGCC 3 cut(s) 33, 152, 369
BsiEI CGRYCG 1 cut(s) 370
BsiHKAI GWGCWC 1 cut(s) 46
BslI CCNNNNNNNGG 1 cut(s) 124
BsnI GGCC 3 cut(s) 33, 152, 369
Bsp120I GGGCCC 1 cut(s) 150
Bsp1286I GDGCHC 2 cut(s) 46, 154
Bsp19I CCATGG 1 cut(s) 28
BspACI CCGC 2 cut(s) 315, 366
BspANI GGCC 3 cut(s) 33, 152, 369
BspCNI CTCAG 2 cut(s) 354, 450
BspLI GGNNCC 3 cut(s) 111, 152, 153
BspTI CTTAAG 1 cut(s) 20
BsrBI CCGCTC 1 cut(s) 366
BsrI ACTGG 2 cut(s) 113, 264
BssECI CCNNGG 3 cut(s) 28, 370, 442
BssSI CACGAG 1 cut(s) 309
BssT1I CCWWGG 1 cut(s) 28
Bst2BI CACGAG 1 cut(s) 309
Bst2UI CCWGG 1 cut(s) 444
BstAFI CTTAAG 1 cut(s) 20
BstAPI GCANNNNNTGC 1 cut(s) 10
BstC8I GCNNGC 2 cut(s) 317, 501
BstDEI CTNAG 2 cut(s) 362, 437
BstDSI CCRYGG 1 cut(s) 28
BstF5I GGATG 1 cut(s) 396
BstMCI CGRYCG 1 cut(s) 370
BstMWI GCNNNNNNNGC 2 cut(s) 10, 372
BstNI CCWGG 1 cut(s) 444
BstNSI RCATGY 1 cut(s) 8
BstSCI CCNGG 1 cut(s) 442
BstSLI GKGCMC 1 cut(s) 154
BstZI CGGCCG 1 cut(s) 367
BsuRI GGCC 3 cut(s) 33, 152, 369
BtgI CCRYGG 1 cut(s) 28
BtsCI GGATG 1 cut(s) 396
Cac8I GCNNGC 2 cut(s) 317, 501
Cfr13I GGNCC 2 cut(s) 150, 151
Csp6I GTAC 1 cut(s) 428
CviAII CATG 2 cut(s) 5, 29
CviJI RGCY 9 cut(s) 33, 49, 85, 112, 152, 211, 369, 375, 441
CviKI_1 RGCY 9 cut(s) 33, 49, 85, 112, 152, 211, 369, 375, 441
CviQI GTAC 1 cut(s) 428
DdeI CTNAG 2 cut(s) 362, 437
EaeI YGGCCR 1 cut(s) 367
EagI CGGCCG 1 cut(s) 367
EclXI CGGCCG 1 cut(s) 367
Eco130I CCWWGG 1 cut(s) 28
Eco24I GRGCYC 1 cut(s) 154
Eco52I CGGCCG 1 cut(s) 367
EcoO109I RGGNCCY 1 cut(s) 151
EcoRII CCWGG 1 cut(s) 442
EcoT14I CCWWGG 1 cut(s) 28
EcoT22I ATGCAT 1 cut(s) 6
EcoT38I GRGCYC 1 cut(s) 154
ErhI CCWWGG 1 cut(s) 28
FaeI CATG 2 cut(s) 8, 32
FatI CATG 2 cut(s) 4, 28
FauI CCCGC 1 cut(s) 308
Fnu4HI GCNGC 2 cut(s) 11, 367
FokI GGATG 1 cut(s) 403
FriOI GRGCYC 1 cut(s) 154
Fsp4HI GCNGC 2 cut(s) 11, 367
FspBI CTAG 2 cut(s) 90, 526
GluI GCNGC 2 cut(s) 11, 367
HaeIII GGCC 3 cut(s) 33, 152, 369
Hin1II CATG 2 cut(s) 8, 32
HphI GGTGA 1 cut(s) 425
Hpy166II GTNNAC 1 cut(s) 256
Hpy188I TCNGA 1 cut(s) 363
Hpy8I GTNNAC 1 cut(s) 256
HpyAV CCTTC 2 cut(s) 128, 132
HpyCH4V TGCA 1 cut(s) 4
HpyF10VI GCNNNNNNNGC 2 cut(s) 10, 372
HpyF3I CTNAG 2 cut(s) 362, 437
Hsp92II CATG 2 cut(s) 8, 32
LmnI GCTCC 2 cut(s) 41, 109
LpnPI CCDG 5 cut(s) 126, 277, 429, 456, 481
LweI GCATC 1 cut(s) 480
MaeI CTAG 2 cut(s) 90, 526
MaeIII GTNAC 2 cut(s) 266, 304
MbiI CCGCTC 1 cut(s) 366
MhlI GDGCHC 2 cut(s) 46, 154
MluCI AATT 1 cut(s) 274
MmeI TCCRAC 1 cut(s) 109
MnlI CCTC 8 cut(s) 44, 98, 125, 244, 305, 334, 365, 446
Mph1103I ATGCAT 1 cut(s) 6
MroXI GAANNNNTTC 1 cut(s) 278
MseI TTAA 2 cut(s) 21, 162
MslI CAYNNNNRTG 1 cut(s) 195
MspCI CTTAAG 1 cut(s) 20
MspR9I CCNGG 1 cut(s) 444
MvaI CCWGG 1 cut(s) 444
MwoI GCNNNNNNNGC 2 cut(s) 10, 372
NcoI CCATGG 1 cut(s) 28
NlaIII CATG 2 cut(s) 8, 32
NlaIV GGNNCC 3 cut(s) 111, 152, 153
NmeAIII GCCGAG 1 cut(s) 395
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 1 cut(s) 8
PcsI WCGNNNNNNNCGW 1 cut(s) 316
PdmI GAANNNNTTC 1 cut(s) 278
PkrI GCNGC 2 cut(s) 12, 368
Psp6I CCWGG 1 cut(s) 442
PspGI CCWGG 1 cut(s) 442
PspN4I GGNNCC 3 cut(s) 111, 152, 153
PspOMI GGGCCC 1 cut(s) 150
PspPI GGNCC 2 cut(s) 150, 151
RsaI GTAC 1 cut(s) 429
RsaNI GTAC 1 cut(s) 428
RseI CAYNNNNRTG 1 cut(s) 195
SaqAI TTAA 2 cut(s) 21, 162
SatI GCNGC 2 cut(s) 11, 367
Sau96I GGNCC 2 cut(s) 150, 151
ScrFI CCNGG 1 cut(s) 444
SduI GDGCHC 2 cut(s) 46, 154
SetI ASST 4 cut(s) 87, 143, 255, 438
SfaNI GCATC 1 cut(s) 480
SmiMI CAYNNNNRTG 1 cut(s) 195
SmlI CTYRAG 2 cut(s) 20, 248
SmoI CTYRAG 2 cut(s) 20, 248
Sse9I AATT 1 cut(s) 274
SsiI CCGC 2 cut(s) 315, 366
SspMI CTAG 2 cut(s) 90, 526
StyD4I CCNGG 1 cut(s) 442
StyI CCWWGG 1 cut(s) 28
TaqI TCGA 1 cut(s) 282
TasI AATT 1 cut(s) 274
TauI GCSGC 1 cut(s) 369
Tru1I TTAA 2 cut(s) 21, 162
Tru9I TTAA 2 cut(s) 21, 162
TseI GCWGC 1 cut(s) 10
TspDTI ATGAA 3 cut(s) 110, 407, 450
Vha464I CTTAAG 1 cut(s) 20
XceI RCATGY 1 cut(s) 8
XcmI CCANNNNNNNNNTGG 1 cut(s) 35
XmnI GAANNNNTTC 1 cut(s) 278
XspI CTAG 2 cut(s) 90, 526
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.