MD11G1182000.v1.1

Lamin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0005650
Physical Location & Seq
Reverse (-)
22419523 .. 22420490
968 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1182000.v1.1.491

Sequence Viewer

Length: 510 bp
ATGAAGGAGGATAAGGGCCTGAAAAGTAGAGGTTGGTGGTTGATGGTTGTGATGATAATACTAAAGGTTGCCGAATGCAGGCAGCCTGTCCTCCATAGGATAGGAGGGGGACGTAACACCTGGGCTCCCCGTATTAACCTCACGCAATGGTCCAGTCAAGAACGCTTCTACGTCGGTGATTGGCTCTATTTTGGGTTTGACAAGCGTATCTACAATGTTCTGGAGGTGAACAGCACAAGCTATGAGAATTGCGTCGACAAAGATTTCATATATAACATCACGAGAGGCGGCCGGGATGTGTTCAATTTGACAGAGGCAAAGACATATTTCTTCCTCAGCGGTCGAGGTTACTGTTTTGAGGGTATGAAAGTTGCAATCCATGTTCAAGAAATCCCACCTGAACAACTTCCTCCAAACTCTGCAAATGATTCCCAATCATACGTCCACAACCACATTACCCTACTTGCAATGCTTGCCATTGCGGCTTCGGCATGGACATTTCCCTACTAG

Protein Analysis

170

Amino Acids

19.57

Weight (kDa)

8.42

Isoelectric Point (pI)

43.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu_bind_like PF02298 41 - 122 1.6e-17 Plastocyanin-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 255
AciI CCGC 3 cut(s) 288, 339, 482
AcoI YGGCCR 1 cut(s) 289
AfiI CCNNNNNNNGG 1 cut(s) 78
AgsI TTSAA 2 cut(s) 304, 386
AjnI CCWGG 1 cut(s) 119
AluBI AGCT 1 cut(s) 240
AluI AGCT 1 cut(s) 240
AoxI GGCC 2 cut(s) 16, 289
ApeKI GCWGC 1 cut(s) 82
Asp700I GAANNNNTTC 1 cut(s) 405
AspS9I GGNCC 2 cut(s) 16, 150
AsuC2I CCSGG 1 cut(s) 293
AsuHPI GGTGA 2 cut(s) 188, 238
AvaII GGWCC 1 cut(s) 150
BaeI ACNNNNGTAYC 2 cut(s) 190, 223
BanII GRGCYC 1 cut(s) 127
BauI CACGAG 1 cut(s) 280
BbvCI CCTCAGC 1 cut(s) 335
BbvI GCAGC 1 cut(s) 94
BccI CCATC 1 cut(s) 37
BciT130I CCWGG 1 cut(s) 121
BcnI CCSGG 1 cut(s) 293
BfaI CTAG 1 cut(s) 508
BglI GCCNNNNNGGC 1 cut(s) 482
BisI GCNGC 3 cut(s) 83, 289, 483
BlsI GCNGC 3 cut(s) 84, 290, 484
Bme1390I CCNGG 2 cut(s) 121, 293
Bme18I GGWCC 1 cut(s) 150
BmgT120I GGNCC 2 cut(s) 16, 150
BmiI GGNNCC 1 cut(s) 126
BmrFI CCNGG 2 cut(s) 121, 293
BpmI CTGGAG 1 cut(s) 242
Bpu10I CCTNAGC 1 cut(s) 335
BpuMI CCSGG 1 cut(s) 293
BsaJI CCNNGG 1 cut(s) 120
BsaXI ACNNNNNCTCC 2 cut(s) 109, 139
Bsc4I CCNNNNNNNGG 1 cut(s) 78
Bse1I ACTGG 1 cut(s) 153
Bse3DI GCAATG 3 cut(s) 152, 474, 477
BseBI CCWGG 1 cut(s) 121
BseDI CCNNGG 1 cut(s) 120
BseGI GGATG 1 cut(s) 301
BseLI CCNNNNNNNGG 1 cut(s) 78
BseMI GCAATG 3 cut(s) 152, 474, 477
BseMII CTCAG 1 cut(s) 349
BseNI ACTGG 1 cut(s) 153
BseX3I CGGCCG 1 cut(s) 289
BseXI GCAGC 1 cut(s) 94
Bsh1285I CGRYCG 2 cut(s) 292, 343
BshFI GGCC 2 cut(s) 18, 291
BsiEI CGRYCG 2 cut(s) 292, 343
BsiSI CCGG 1 cut(s) 292
BslFI GGGAC 1 cut(s) 123
BslI CCNNNNNNNGG 1 cut(s) 78
BsmFI GGGAC 1 cut(s) 123
BsmI GAATGC 1 cut(s) 80
BsnI GGCC 2 cut(s) 18, 291
Bsp1286I GDGCHC 1 cut(s) 127
BspACI CCGC 3 cut(s) 288, 339, 482
BspANI GGCC 2 cut(s) 18, 291
BspCNI CTCAG 1 cut(s) 348
BspLI GGNNCC 1 cut(s) 126
BsrDI GCAATG 3 cut(s) 152, 474, 477
BsrI ACTGG 1 cut(s) 153
BssECI CCNNGG 1 cut(s) 120
BssSI CACGAG 1 cut(s) 280
Bst2BI CACGAG 1 cut(s) 280
Bst2UI CCWGG 1 cut(s) 121
Bst4CI ACNGT 1 cut(s) 353
BstAPI GCANNNNNTGC 1 cut(s) 473
BstC8I GCNNGC 2 cut(s) 80, 474
BstDEI CTNAG 1 cut(s) 335
BstF5I GGATG 1 cut(s) 301
BstMCI CGRYCG 2 cut(s) 292, 343
BstMWI GCNNNNNNNGC 3 cut(s) 473, 482, 488
BstNI CCWGG 1 cut(s) 121
BstSCI CCNGG 2 cut(s) 119, 291
BstV1I GCAGC 1 cut(s) 94
BstZI CGGCCG 1 cut(s) 289
BsuRI GGCC 2 cut(s) 18, 291
BtsCI GGATG 1 cut(s) 301
Cac8I GCNNGC 2 cut(s) 80, 474
Cfr13I GGNCC 2 cut(s) 16, 150
CseI GACGC 1 cut(s) 241
CviAII CATG 2 cut(s) 380, 492
CviJI RGCY 7 cut(s) 18, 85, 125, 184, 240, 291, 485
CviKI_1 RGCY 7 cut(s) 18, 85, 125, 184, 240, 291, 485
DdeI CTNAG 1 cut(s) 335
EaeI YGGCCR 1 cut(s) 289
EagI CGGCCG 1 cut(s) 289
EclXI CGGCCG 1 cut(s) 289
Eco24I GRGCYC 1 cut(s) 127
Eco47I GGWCC 1 cut(s) 150
Eco52I CGGCCG 1 cut(s) 289
EcoO109I RGGNCCY 1 cut(s) 16
EcoRII CCWGG 1 cut(s) 119
EcoT38I GRGCYC 1 cut(s) 127
FaeI CATG 2 cut(s) 383, 495
FaqI GGGAC 1 cut(s) 123
FatI CATG 2 cut(s) 379, 491
FblI GTMKAC 1 cut(s) 255
Fnu4HI GCNGC 3 cut(s) 83, 289, 483
FokI GGATG 1 cut(s) 308
FriOI GRGCYC 1 cut(s) 127
Fsp4HI GCNGC 3 cut(s) 83, 289, 483
FspBI CTAG 1 cut(s) 508
GluI GCNGC 3 cut(s) 83, 289, 483
GsuI CTGGAG 1 cut(s) 242
HaeIII GGCC 2 cut(s) 18, 291
HapII CCGG 1 cut(s) 292
HgaI GACGC 1 cut(s) 241
Hin1II CATG 2 cut(s) 383, 495
HincII GTYRAC 1 cut(s) 256
HindII GTYRAC 1 cut(s) 256
HinfI GANTC 1 cut(s) 428
HpaII CCGG 1 cut(s) 292
HphI GGTGA 2 cut(s) 188, 238
Hpy166II GTNNAC 3 cut(s) 229, 256, 445
Hpy188III TCNNGA 4 cut(s) 158, 221, 280, 386
Hpy8I GTNNAC 3 cut(s) 229, 256, 445
Hpy99I CGWCG 2 cut(s) 176, 257
HpyCH4III ACNGT 1 cut(s) 353
HpyCH4IV ACGT 3 cut(s) 112, 171, 441
HpyCH4V TGCA 4 cut(s) 78, 374, 422, 467
HpyF10VI GCNNNNNNNGC 3 cut(s) 473, 482, 488
HpyF3I CTNAG 1 cut(s) 335
HpySE526I ACGT 3 cut(s) 112, 171, 441
Hsp92II CATG 2 cut(s) 383, 495
LmnI GCTCC 1 cut(s) 130
LpnPI CCDG 9 cut(s) 32, 64, 99, 106, 133, 166, 206, 305, 411
Lsp1109I GCAGC 1 cut(s) 94
MaeI CTAG 1 cut(s) 508
MaeII ACGT 3 cut(s) 112, 171, 441
MaeIII GTNAC 2 cut(s) 113, 347
MboII GAAGA 1 cut(s) 322
MhlI GDGCHC 1 cut(s) 127
MluCI AATT 2 cut(s) 247, 304
MroXI GAANNNNTTC 1 cut(s) 405
MseI TTAA 1 cut(s) 135
MspA1I CMGCKG 1 cut(s) 339
MspI CCGG 1 cut(s) 292
MspR9I CCNGG 2 cut(s) 121, 293
Mva1269I GAATGC 1 cut(s) 80
MvaI CCWGG 1 cut(s) 121
MwoI GCNNNNNNNGC 3 cut(s) 473, 482, 488
NciI CCSGG 1 cut(s) 293
NlaIII CATG 2 cut(s) 383, 495
NlaIV GGNNCC 1 cut(s) 126
PctI GAATGC 1 cut(s) 80
PdmI GAANNNNTTC 1 cut(s) 405
PfeI GAWTC 1 cut(s) 428
PkrI GCNGC 3 cut(s) 84, 290, 484
Psp6I CCWGG 1 cut(s) 119
PspGI CCWGG 1 cut(s) 119
PspN4I GGNNCC 1 cut(s) 126
PspPI GGNCC 2 cut(s) 16, 150
SalI GTCGAC 1 cut(s) 254
SaqAI TTAA 1 cut(s) 135
SatI GCNGC 3 cut(s) 83, 289, 483
Sau96I GGNCC 2 cut(s) 16, 150
ScrFI CCNGG 2 cut(s) 121, 293
SduI GDGCHC 1 cut(s) 127
SinI GGWCC 1 cut(s) 150
Sse9I AATT 2 cut(s) 247, 304
SsiI CCGC 3 cut(s) 288, 339, 482
SspMI CTAG 1 cut(s) 508
StyD4I CCNGG 2 cut(s) 119, 291
TaaI ACNGT 1 cut(s) 353
TaiI ACGT 3 cut(s) 115, 174, 444
TaqI TCGA 2 cut(s) 255, 343
TasI AATT 2 cut(s) 247, 304
TauI GCSGC 2 cut(s) 291, 485
TfiI GAWTC 1 cut(s) 428
Tru1I TTAA 1 cut(s) 135
Tru9I TTAA 1 cut(s) 135
TseI GCWGC 1 cut(s) 82
TspDTI ATGAA 3 cut(s) 17, 256, 380
VpaK11BI GGWCC 1 cut(s) 150
XmiI GTMKAC 1 cut(s) 255
XmnI GAANNNNTTC 1 cut(s) 405
XspI CTAG 1 cut(s) 508
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.