MD17G1054300.v1.1

Lamin-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0005650
Physical Location & Seq
Reverse (-)
4211617 .. 4212709
1093 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1054300.v1.1.491

Sequence Viewer

Length: 519 bp
ATGCGGATGATGAATTCGGGAGTGATGGTGGCCGTGGTAATAACGATGGGGTGGCTAATGGCGTCTGCAACCGCTATTCTGCATAAGGTGGGAGGCAAACAGGGTTGGGATCAGAACGTCAATTACACTGAGTGGGCGGCAAATGAACAGGTTTACGTCGGCGAATGGCTCATATTCAACTTTGACAAGAGGTACTACAATGTTCTGGAGGTGAACAAGACGAGCTACGAGAACTGCAATGATCAAGGCTTCATAAAGAACATAACCCGGGGAGGGCGAGATGTGTATCAACTCACAGAGGCAAGGCCGTATTACTTCCTCTCCGGCGGAGGTTACTGCTGGCATGGCATGAAGCTTTATCTGACCGTCCACGATGGGCTCCCACCTGCTCCAGAACTCGCTCCAGCACTTGCTCCGGCTACAAGTGTGGCTTGTCCTCCAAATTCACTAACCTCCTCCGCCGCTGAGATGTTGTTTCTATCCATTGCGCTTTGCTTAGCTTTAATGTGGACGCTCTAG

Protein Analysis

173

Amino Acids

19.14

Weight (kDa)

5.87

Isoelectric Point (pI)

39.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu_bind_like PF02298 36 - 117 3.3e-17 Plastocyanin-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 394
Acc36I ACCTGC 1 cut(s) 394
AciI CCGC 6 cut(s) 4, 72, 137, 327, 459, 462
AclWI GGATC 1 cut(s) 117
AcoI YGGCCR 1 cut(s) 30
AcsI RAATTY 2 cut(s) 13, 442
AcyI GRCGYC 1 cut(s) 62
AdeI CACNNNGTG 1 cut(s) 132
AfaI GTAC 1 cut(s) 194
AfiI CCNNNNNNNGG 1 cut(s) 273
AgsI TTSAA 1 cut(s) 178
AluBI AGCT 3 cut(s) 225, 355, 500
AluI AGCT 3 cut(s) 225, 355, 500
AlwI GGATC 1 cut(s) 117
Ama87I CYCGRG 1 cut(s) 267
AoxI GGCC 2 cut(s) 30, 305
ApoI RAATTY 2 cut(s) 13, 442
AspLEI GCGC 1 cut(s) 490
AsuC2I CCSGG 2 cut(s) 268, 269
AsuHPI GGTGA 1 cut(s) 223
AvaI CYCGRG 1 cut(s) 267
BanII GRGCYC 1 cut(s) 381
BccI CCATC 3 cut(s) 19, 40, 368
BceAI ACGGC 2 cut(s) 17, 292
BclI TGATCA 1 cut(s) 241
BcnI CCSGG 2 cut(s) 268, 269
BfaI CTAG 1 cut(s) 517
BfuAI ACCTGC 1 cut(s) 394
BisI GCNGC 2 cut(s) 138, 462
BlpI GCTNAGC 1 cut(s) 496
BlsI GCNGC 2 cut(s) 139, 463
Bme1390I CCNGG 2 cut(s) 268, 269
BmeT110I CYCGRG 1 cut(s) 267
BmiI GGNNCC 1 cut(s) 380
BmrFI CCNGG 2 cut(s) 268, 269
BpmI CTGGAG 3 cut(s) 227, 375, 387
Bpu1102I GCTNAGC 1 cut(s) 496
BpuMI CCSGG 2 cut(s) 268, 269
BsaBI GATNNNNATC 1 cut(s) 285
BsaHI GRCGYC 1 cut(s) 62
BsaJI CCNNGG 3 cut(s) 33, 267, 268
BsaXI ACNNNNNCTCC 4 cut(s) 12, 42, 305, 335
Bsc4I CCNNNNNNNGG 1 cut(s) 273
Bse3DI GCAATG 2 cut(s) 244, 483
Bse8I GATNNNNATC 1 cut(s) 285
BseDI CCNNGG 3 cut(s) 33, 267, 268
BseGI GGATG 1 cut(s) 12
BseJI GATNNNNATC 1 cut(s) 285
BseLI CCNNNNNNNGG 1 cut(s) 273
BseMI GCAATG 2 cut(s) 244, 483
BseMII CTCAG 2 cut(s) 120, 456
BseRI GAGGAG 1 cut(s) 445
BshFI GGCC 2 cut(s) 32, 307
BsiHKCI CYCGRG 1 cut(s) 267
BsiSI CCGG 3 cut(s) 268, 324, 416
BslI CCNNNNNNNGG 1 cut(s) 273
BsnI GGCC 2 cut(s) 32, 307
BsoBI CYCGRG 1 cut(s) 267
Bsp1286I GDGCHC 1 cut(s) 381
Bsp143I GATC 2 cut(s) 109, 241
Bsp1720I GCTNAGC 1 cut(s) 496
BspACI CCGC 6 cut(s) 4, 72, 137, 327, 459, 462
BspANI GGCC 2 cut(s) 32, 307
BspCNI CTCAG 2 cut(s) 121, 457
BspLI GGNNCC 1 cut(s) 380
BspMI ACCTGC 1 cut(s) 394
BspPI GGATC 1 cut(s) 117
BsrDI GCAATG 2 cut(s) 244, 483
BssECI CCNNGG 3 cut(s) 33, 267, 268
BssMI GATC 2 cut(s) 109, 241
BssNI GRCGYC 1 cut(s) 62
Bst4CI ACNGT 1 cut(s) 367
BstACI GRCGYC 1 cut(s) 62
BstC8I GCNNGC 1 cut(s) 341
BstDEI CTNAG 3 cut(s) 129, 465, 496
BstDSI CCRYGG 1 cut(s) 33
BstF5I GGATG 1 cut(s) 12
BstHHI GCGC 1 cut(s) 490
BstKTI GATC 2 cut(s) 112, 244
BstMBI GATC 2 cut(s) 109, 241
BstMWI GCNNNNNNNGC 1 cut(s) 345
BstSCI CCNGG 2 cut(s) 266, 267
BsuRI GGCC 2 cut(s) 32, 307
BtgI CCRYGG 1 cut(s) 33
BtsCI GGATG 1 cut(s) 12
BtsIMutI CAGTG 1 cut(s) 126
BveI ACCTGC 1 cut(s) 394
Cac8I GCNNGC 1 cut(s) 341
CfoI GCGC 1 cut(s) 490
Cfr9I CCCGGG 1 cut(s) 267
CseI GACGC 1 cut(s) 51
Csp6I GTAC 1 cut(s) 193
CviAII CATG 2 cut(s) 344, 349
CviQI GTAC 1 cut(s) 193
DdeI CTNAG 3 cut(s) 129, 465, 496
DpnI GATC 2 cut(s) 111, 243
DpnII GATC 2 cut(s) 109, 241
DraIII CACNNNGTG 1 cut(s) 132
EaeI YGGCCR 1 cut(s) 30
EciI GGCGGA 2 cut(s) 342, 448
Eco24I GRGCYC 1 cut(s) 381
Eco88I CYCGRG 1 cut(s) 267
EcoRI GAATTC 1 cut(s) 13
EcoT38I GRGCYC 1 cut(s) 381
FaeI CATG 2 cut(s) 347, 352
FaiI YATR 6 cut(s) 84, 173, 254, 263, 345, 350
FalI AAGNNNNNCTT 2 cut(s) 415, 447
FatI CATG 2 cut(s) 343, 348
FbaI TGATCA 1 cut(s) 241
Fnu4HI GCNGC 2 cut(s) 138, 462
FokI GGATG 1 cut(s) 19
FriOI GRGCYC 1 cut(s) 381
Fsp4HI GCNGC 2 cut(s) 138, 462
FspBI CTAG 1 cut(s) 517
GlaI GCGC 1 cut(s) 489
GluI GCNGC 2 cut(s) 138, 462
GsuI CTGGAG 3 cut(s) 227, 375, 387
HaeIII GGCC 2 cut(s) 32, 307
HapII CCGG 3 cut(s) 268, 324, 416
HgaI GACGC 1 cut(s) 51
HhaI GCGC 1 cut(s) 490
Hin1I GRCGYC 1 cut(s) 62
Hin1II CATG 2 cut(s) 347, 352
Hin6I GCGC 1 cut(s) 488
HinP1I GCGC 1 cut(s) 488
HindIII AAGCTT 1 cut(s) 353
HpaII CCGG 3 cut(s) 268, 324, 416
HphI GGTGA 1 cut(s) 223
Hpy166II GTNNAC 4 cut(s) 154, 214, 370, 510
Hpy188I TCNGA 2 cut(s) 114, 363
Hpy188III TCNNGA 3 cut(s) 18, 206, 392
Hpy8I GTNNAC 4 cut(s) 154, 214, 370, 510
Hpy99I CGWCG 1 cut(s) 161
HpyCH4III ACNGT 1 cut(s) 367
HpyCH4IV ACGT 2 cut(s) 117, 156
HpyCH4V TGCA 3 cut(s) 68, 82, 237
HpyF10VI GCNNNNNNNGC 1 cut(s) 345
HpyF3I CTNAG 3 cut(s) 129, 465, 496
HpySE526I ACGT 2 cut(s) 117, 156
Hsp92I GRCGYC 1 cut(s) 62
Hsp92II CATG 2 cut(s) 347, 352
HspAI GCGC 1 cut(s) 488
Ksp22I TGATCA 1 cut(s) 241
Kzo9I GATC 2 cut(s) 109, 241
LmnI GCTCC 4 cut(s) 384, 394, 406, 418
MaeI CTAG 1 cut(s) 517
MaeII ACGT 2 cut(s) 117, 156
MaeIII GTNAC 1 cut(s) 332
MalI GATC 2 cut(s) 111, 243
MboI GATC 2 cut(s) 109, 241
MhlI GDGCHC 1 cut(s) 381
MluCI AATT 3 cut(s) 13, 121, 442
MseI TTAA 1 cut(s) 503
MspA1I CMGCKG 1 cut(s) 464
MspI CCGG 3 cut(s) 268, 324, 416
MspR9I CCNGG 2 cut(s) 268, 269
MwoI GCNNNNNNNGC 1 cut(s) 345
NciI CCSGG 2 cut(s) 268, 269
NdeII GATC 2 cut(s) 109, 241
NlaIII CATG 2 cut(s) 347, 352
NlaIV GGNNCC 1 cut(s) 380
PaqCI CACCTGC 1 cut(s) 394
PkrI GCNGC 2 cut(s) 139, 463
PspN4I GGNNCC 1 cut(s) 380
PsrI GAACNNNNNNTAC 2 cut(s) 107, 139
RsaI GTAC 1 cut(s) 194
RsaNI GTAC 1 cut(s) 193
SaqAI TTAA 1 cut(s) 503
SatI GCNGC 2 cut(s) 138, 462
Sau3AI GATC 2 cut(s) 109, 241
ScrFI CCNGG 2 cut(s) 268, 269
SduI GDGCHC 1 cut(s) 381
SmaI CCCGGG 1 cut(s) 269
Sse9I AATT 3 cut(s) 13, 121, 442
SsiI CCGC 6 cut(s) 4, 72, 137, 327, 459, 462
SspMI CTAG 1 cut(s) 517
StyD4I CCNGG 2 cut(s) 266, 267
TaaI ACNGT 1 cut(s) 367
TaiI ACGT 2 cut(s) 120, 159
TasI AATT 3 cut(s) 13, 121, 442
TauI GCSGC 2 cut(s) 140, 464
Tru1I TTAA 1 cut(s) 503
Tru9I TTAA 1 cut(s) 503
TscAI CASTG 1 cut(s) 133
TspDTI ATGAA 4 cut(s) 26, 159, 241, 365
TspMI CCCGGG 1 cut(s) 267
TspRI CASTG 1 cut(s) 133
XapI RAATTY 2 cut(s) 13, 442
XmaI CCCGGG 1 cut(s) 267
XspI CTAG 1 cut(s) 517
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.