Rroxscaffold_2G00083920

Lamin-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0005650
Physical Location & Seq
Reverse (-)
6460893 .. 6462236
1344 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00083920.1

Sequence Viewer

Length: 501 bp
ATGATGAGGATTAGAATGGTGACGGTGACGGTGATGGTGGTGGTGGTGGTGGGGTTTCTGACGACGAGCGCGGAGGCCAAGCTGCATAAGGTGGGAGACAAACAAGGATGGAATCCGAACGTCAATTACACTCAATGGTCCGCCGATAAGGAATTTTACGTCGGTGAATGGCTCATATTTAATTTCGACAAGAGGTACTACACTGCGCTGGAGGTGAACAAGACCAGTTTTGACAGCTGCAATGACAAAGTTTTCATAACCAACATCACCCGTGGCGGCCGAGATGTGTTTGAACTGAAAGAGGCTAGGCCATACTACTTCCTCTCAAGTGGAGGATACTGCTTCCATGGAATGAAACTTGCTCTTGTTGCCCAAGAACAGCCCCCGCTTGCTCCGGCGACTCCAGCCCTGGGTCTTCTCCAATTCATTAACCTCTTGCGCTCAATCCATTCTGTCCCTCATTGTGGTTTCCTTTGCTTTGGTGACAAACTTTGGTTGTAG

Protein Analysis

166

Amino Acids

18.9

Weight (kDa)

8.67

Isoelectric Point (pI)

34.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu_bind_like PF02298 37 - 118 6.2e-16 Plastocyanin-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 71
AciI CCGC 4 cut(s) 71, 141, 276, 386
AcoI YGGCCR 1 cut(s) 277
AcsI RAATTY 1 cut(s) 152
AfaI GTAC 1 cut(s) 197
AfiI CCNNNNNNNGG 2 cut(s) 410, 464
AgsI TTSAA 1 cut(s) 293
AjnI CCWGG 1 cut(s) 408
AluBI AGCT 2 cut(s) 82, 237
AluI AGCT 2 cut(s) 82, 237
Alw26I GTCTC 1 cut(s) 90
AoxI GGCC 3 cut(s) 75, 277, 308
ApeKI GCWGC 2 cut(s) 82, 237
ApoI RAATTY 1 cut(s) 152
AspLEI GCGC 3 cut(s) 71, 208, 441
AspS9I GGNCC 1 cut(s) 138
AsuHPI GGTGA 7 cut(s) 31, 37, 43, 176, 226, 259, 494
AvaII GGWCC 1 cut(s) 138
BbsI GAAGAC 1 cut(s) 407
BbvI GCAGC 2 cut(s) 69, 224
BccI CCATC 2 cut(s) 28, 102
BciT130I CCWGG 1 cut(s) 410
BciVI GTATCC 1 cut(s) 329
BcoDI GTCTC 1 cut(s) 90
BfaI CTAG 1 cut(s) 306
BfuI GTATCC 1 cut(s) 329
BisI GCNGC 3 cut(s) 83, 238, 277
BlsI GCNGC 3 cut(s) 84, 239, 278
Bme1390I CCNGG 1 cut(s) 410
Bme18I GGWCC 1 cut(s) 138
BmgT120I GGNCC 1 cut(s) 138
BmrFI CCNGG 1 cut(s) 410
BpiI GAAGAC 1 cut(s) 407
BpmI CTGGAG 2 cut(s) 230, 387
BpuEI CTTGAG 1 cut(s) 310
BsaJI CCNNGG 4 cut(s) 271, 346, 408, 409
Bsc4I CCNNNNNNNGG 2 cut(s) 410, 464
Bse1I ACTGG 1 cut(s) 225
Bse3DI GCAATG 1 cut(s) 247
BseBI CCWGG 1 cut(s) 410
BseDI CCNNGG 4 cut(s) 271, 346, 408, 409
BseGI GGATG 1 cut(s) 113
BseLI CCNNNNNNNGG 2 cut(s) 410, 464
BseMI GCAATG 1 cut(s) 247
BseNI ACTGG 1 cut(s) 225
BseX3I CGGCCG 1 cut(s) 277
BseXI GCAGC 2 cut(s) 69, 224
Bsh1236I CGCG 1 cut(s) 71
Bsh1285I CGRYCG 1 cut(s) 280
BshFI GGCC 3 cut(s) 77, 279, 310
BsiEI CGRYCG 1 cut(s) 280
BsiSI CCGG 1 cut(s) 395
BslFI GGGAC 1 cut(s) 440
BslI CCNNNNNNNGG 2 cut(s) 410, 464
BsmAI GTCTC 1 cut(s) 90
BsmFI GGGAC 1 cut(s) 440
BsnI GGCC 3 cut(s) 77, 279, 310
Bsp19I CCATGG 1 cut(s) 346
BspACI CCGC 4 cut(s) 71, 141, 276, 386
BspANI GGCC 3 cut(s) 77, 279, 310
BspFNI CGCG 1 cut(s) 71
BsrDI GCAATG 1 cut(s) 247
BsrI ACTGG 1 cut(s) 225
BssECI CCNNGG 4 cut(s) 271, 346, 408, 409
BssT1I CCWWGG 1 cut(s) 346
Bst2UI CCWGG 1 cut(s) 410
Bst4CI ACNGT 2 cut(s) 25, 31
BstC8I GCNNGC 1 cut(s) 390
BstDSI CCRYGG 2 cut(s) 271, 346
BstF5I GGATG 1 cut(s) 113
BstFNI CGCG 1 cut(s) 71
BstHHI GCGC 3 cut(s) 71, 208, 441
BstMAI GTCTC 1 cut(s) 90
BstMCI CGRYCG 1 cut(s) 280
BstMWI GCNNNNNNNGC 2 cut(s) 368, 404
BstNI CCWGG 1 cut(s) 410
BstSCI CCNGG 1 cut(s) 408
BstUI CGCG 1 cut(s) 71
BstV1I GCAGC 2 cut(s) 69, 224
BstV2I GAAGAC 1 cut(s) 407
BstZI CGGCCG 1 cut(s) 277
BsuI GTATCC 1 cut(s) 329
BsuRI GGCC 3 cut(s) 77, 279, 310
BtgI CCRYGG 2 cut(s) 271, 346
BtsCI GGATG 1 cut(s) 113
BtsI GCAGTG 1 cut(s) 201
BtsIMutI CAGTG 1 cut(s) 201
Cac8I GCNNGC 1 cut(s) 390
CfoI GCGC 3 cut(s) 71, 208, 441
Cfr13I GGNCC 1 cut(s) 138
Csp6I GTAC 1 cut(s) 196
CviAII CATG 1 cut(s) 347
CviJI RGCY 9 cut(s) 77, 82, 172, 237, 279, 305, 310, 382, 407
CviKI_1 RGCY 9 cut(s) 77, 82, 172, 237, 279, 305, 310, 382, 407
CviQI GTAC 1 cut(s) 196
EaeI YGGCCR 1 cut(s) 277
EagI CGGCCG 1 cut(s) 277
EciI GGCGGA 1 cut(s) 130
EclXI CGGCCG 1 cut(s) 277
Eco130I CCWWGG 1 cut(s) 346
Eco47I GGWCC 1 cut(s) 138
Eco52I CGGCCG 1 cut(s) 277
EcoRII CCWGG 1 cut(s) 408
EcoT14I CCWWGG 1 cut(s) 346
ErhI CCWWGG 1 cut(s) 346
FaeI CATG 1 cut(s) 350
FaiI YATR 5 cut(s) 87, 176, 257, 313, 348
FaqI GGGAC 1 cut(s) 440
FatI CATG 1 cut(s) 346
FauI CCCGC 1 cut(s) 393
Fnu4HI GCNGC 3 cut(s) 83, 238, 277
FokI GGATG 1 cut(s) 120
Fsp4HI GCNGC 3 cut(s) 83, 238, 277
FspBI CTAG 1 cut(s) 306
GlaI GCGC 3 cut(s) 70, 207, 440
GluI GCNGC 3 cut(s) 83, 238, 277
GsuI CTGGAG 2 cut(s) 230, 387
HaeIII GGCC 3 cut(s) 77, 279, 310
HapII CCGG 1 cut(s) 395
HhaI GCGC 3 cut(s) 71, 208, 441
Hin1II CATG 1 cut(s) 350
Hin6I GCGC 3 cut(s) 69, 206, 439
HinP1I GCGC 3 cut(s) 69, 206, 439
HinfI GANTC 2 cut(s) 112, 400
HpaII CCGG 1 cut(s) 395
HphI GGTGA 7 cut(s) 31, 37, 43, 176, 226, 259, 494
Hpy166II GTNNAC 1 cut(s) 217
Hpy188I TCNGA 2 cut(s) 60, 117
Hpy8I GTNNAC 1 cut(s) 217
Hpy99I CGWCG 2 cut(s) 67, 164
HpyCH4III ACNGT 2 cut(s) 25, 31
HpyCH4IV ACGT 2 cut(s) 120, 159
HpyCH4V TGCA 2 cut(s) 85, 240
HpyF10VI GCNNNNNNNGC 2 cut(s) 368, 404
HpySE526I ACGT 2 cut(s) 120, 159
Hsp92II CATG 1 cut(s) 350
HspAI GCGC 3 cut(s) 69, 206, 439
LmnI GCTCC 1 cut(s) 397
LpnPI CCDG 6 cut(s) 194, 238, 395, 408, 417, 422
Lsp1109I GCAGC 2 cut(s) 69, 224
MaeI CTAG 1 cut(s) 306
MaeII ACGT 2 cut(s) 120, 159
MaeIII GTNAC 3 cut(s) 19, 25, 482
MboII GAAGA 1 cut(s) 407
MluCI AATT 4 cut(s) 124, 152, 181, 422
MlyI GAGTC 1 cut(s) 394
MnlI CCTC 8 cut(s) 67, 186, 205, 295, 326, 332, 443, 468
MseI TTAA 2 cut(s) 180, 429
MspA1I CMGCKG 1 cut(s) 237
MspI CCGG 1 cut(s) 395
MspR9I CCNGG 1 cut(s) 410
MvaI CCWGG 1 cut(s) 410
MvnI CGCG 1 cut(s) 71
MwoI GCNNNNNNNGC 2 cut(s) 368, 404
NcoI CCATGG 1 cut(s) 346
NlaIII CATG 1 cut(s) 350
NmeAIII GCCGAG 1 cut(s) 305
NmuCI GTSAC 3 cut(s) 19, 25, 482
PasI CCCWGGG 1 cut(s) 409
PfeI GAWTC 1 cut(s) 112
PkrI GCNGC 3 cut(s) 84, 239, 278
PleI GAGTC 1 cut(s) 394
PpsI GAGTC 1 cut(s) 394
Psp6I CCWGG 1 cut(s) 408
PspGI CCWGG 1 cut(s) 408
PspPI GGNCC 1 cut(s) 138
PsrI GAACNNNNNNTAC 2 cut(s) 110, 142
PvuII CAGCTG 1 cut(s) 237
RsaI GTAC 1 cut(s) 197
RsaNI GTAC 1 cut(s) 196
SaqAI TTAA 2 cut(s) 180, 429
SatI GCNGC 3 cut(s) 83, 238, 277
Sau96I GGNCC 1 cut(s) 138
SchI GAGTC 1 cut(s) 394
ScrFI CCNGG 1 cut(s) 410
SetI ASST 8 cut(s) 84, 93, 123, 162, 197, 216, 239, 435
SinI GGWCC 1 cut(s) 138
SmlI CTYRAG 1 cut(s) 325
SmoI CTYRAG 1 cut(s) 325
Sse9I AATT 4 cut(s) 124, 152, 181, 422
SsiI CCGC 4 cut(s) 71, 141, 276, 386
SspMI CTAG 1 cut(s) 306
StyD4I CCNGG 1 cut(s) 408
StyI CCWWGG 1 cut(s) 346
TaaI ACNGT 2 cut(s) 25, 31
TaiI ACGT 2 cut(s) 123, 162
TaqI TCGA 1 cut(s) 186
TasI AATT 4 cut(s) 124, 152, 181, 422
TauI GCSGC 1 cut(s) 279
TfiI GAWTC 1 cut(s) 112
Tru1I TTAA 2 cut(s) 180, 429
Tru9I TTAA 2 cut(s) 180, 429
TscAI CASTG 1 cut(s) 208
TseFI GTSAC 3 cut(s) 19, 25, 482
TseI GCWGC 2 cut(s) 82, 237
Tsp45I GTSAC 3 cut(s) 19, 25, 482
TspDTI ATGAA 3 cut(s) 244, 368, 415
TspRI CASTG 1 cut(s) 208
VpaK11BI GGWCC 1 cut(s) 138
XapI RAATTY 1 cut(s) 152
XspI CTAG 1 cut(s) 306
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.