Rw5G031020

Lamin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0005650
Physical Location & Seq
Forward (+)
50194198 .. 50194761
564 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G031020.1

Sequence Viewer

Length: 489 bp
ATGCTAGTGATAGTAATAATGATGATACTAATGTCGCATGCAGTTGAATCCAGGGAGCCAGTCCTTCATAGGGTTGGAGGGGGAAGGTTCACTTGGGCCCCCGATATTAACTTCACAGATTGGTCAAGTAAAGAGCATTTCTATGTAGGCGATTGGCTCTATTTTGGGTTTGACAAACATATCTACAATGTGCTTGAGGTAAACAGGACCAGTTACGAGAATTGCTTCGACAAAGAATACATATACAATGTTACACGAGGCGGGCGTGATGTGTACAACTTGACAGAGGCAAAGACATACTACTTTCTGAGCGGCCGAGGCTATTGCTACAAAGGGATGAAAGTTGCTGTTTTTGTTGAGCAAACCCCACCCGTACTTCACCTCAGCCCTGGTTTCAAATCACATCCATATTCCAGCACCAACTTTATCATCATTCTCACTATGCTTGCCCATTTCTCACTAGAAAAATTTCACTCTCCCCTCAGTTGA

Protein Analysis

162

Amino Acids

18.87

Weight (kDa)

7.09

Isoelectric Point (pI)

28.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu_bind_like PF02298 32 - 113 8.1e-18 Plastocyanin-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 312
AciI CCGC 2 cut(s) 261, 312
AcoI YGGCCR 1 cut(s) 313
AcsI RAATTY 1 cut(s) 467
AfaI GTAC 2 cut(s) 275, 375
AfiI CCNNNNNNNGG 1 cut(s) 70
AgsI TTSAA 2 cut(s) 47, 397
AjnI CCWGG 2 cut(s) 50, 388
AjuI GAANNNNNNNTTGG 2 cut(s) 76, 108
AoxI GGCC 2 cut(s) 96, 313
ApaI GGGCCC 1 cut(s) 100
ApoI RAATTY 1 cut(s) 467
Asp700I GAANNNNTTC 2 cut(s) 224, 468
AspS9I GGNCC 3 cut(s) 96, 97, 207
AsuHPI GGTGA 1 cut(s) 371
AvaII GGWCC 1 cut(s) 207
BaeGI GKGCMC 1 cut(s) 100
BaeI ACNNNNGTAYC 2 cut(s) 17, 50
BanII GRGCYC 1 cut(s) 100
BauI CACGAG 1 cut(s) 255
BbvCI CCTCAGC 1 cut(s) 383
BcgI CGANNNNNNTGC 2 cut(s) 306, 340
BciT130I CCWGG 2 cut(s) 52, 390
BfaI CTAG 2 cut(s) 5, 461
BisI GCNGC 1 cut(s) 313
BlsI GCNGC 1 cut(s) 314
Bme1390I CCNGG 2 cut(s) 52, 390
Bme18I GGWCC 1 cut(s) 207
BmgT120I GGNCC 3 cut(s) 96, 97, 207
BmiI GGNNCC 3 cut(s) 57, 98, 99
BmrFI CCNGG 2 cut(s) 52, 390
Bpu10I CCTNAGC 1 cut(s) 383
BpuEI CTTGAG 1 cut(s) 215
BsaJI CCNNGG 3 cut(s) 51, 316, 388
Bsc4I CCNNNNNNNGG 1 cut(s) 70
Bse1I ACTGG 2 cut(s) 59, 210
BseBI CCWGG 2 cut(s) 52, 390
BseDI CCNNGG 3 cut(s) 51, 316, 388
BseGI GGATG 2 cut(s) 342, 403
BseLI CCNNNNNNNGG 1 cut(s) 70
BseMII CTCAG 2 cut(s) 299, 397
BseNI ACTGG 2 cut(s) 59, 210
BseSI GKGCMC 1 cut(s) 100
BseX3I CGGCCG 1 cut(s) 313
Bsh1285I CGRYCG 1 cut(s) 316
BshFI GGCC 2 cut(s) 98, 315
BsiEI CGRYCG 1 cut(s) 316
BslI CCNNNNNNNGG 1 cut(s) 70
BsnI GGCC 2 cut(s) 98, 315
Bsp120I GGGCCC 1 cut(s) 96
Bsp1286I GDGCHC 1 cut(s) 100
Bsp1407I TGTACA 1 cut(s) 273
BspACI CCGC 2 cut(s) 261, 312
BspANI GGCC 2 cut(s) 98, 315
BspCNI CTCAG 2 cut(s) 300, 396
BspLI GGNNCC 3 cut(s) 57, 98, 99
BsrBI CCGCTC 1 cut(s) 312
BsrGI TGTACA 1 cut(s) 273
BsrI ACTGG 2 cut(s) 59, 210
BssECI CCNNGG 3 cut(s) 51, 316, 388
BssSI CACGAG 1 cut(s) 255
Bst2BI CACGAG 1 cut(s) 255
Bst2UI CCWGG 2 cut(s) 52, 390
BstAUI TGTACA 1 cut(s) 273
BstC8I GCNNGC 3 cut(s) 39, 263, 447
BstDEI CTNAG 3 cut(s) 308, 383, 482
BstF5I GGATG 2 cut(s) 342, 403
BstMCI CGRYCG 1 cut(s) 316
BstMWI GCNNNNNNNGC 1 cut(s) 318
BstNI CCWGG 2 cut(s) 52, 390
BstNSI RCATGY 1 cut(s) 41
BstSCI CCNGG 2 cut(s) 50, 388
BstSLI GKGCMC 1 cut(s) 100
BstZI CGGCCG 1 cut(s) 313
BsuRI GGCC 2 cut(s) 98, 315
BtsCI GGATG 2 cut(s) 342, 403
Cac8I GCNNGC 3 cut(s) 39, 263, 447
Cfr13I GGNCC 3 cut(s) 96, 97, 207
Csp6I GTAC 2 cut(s) 274, 374
CviAII CATG 1 cut(s) 38
CviJI RGCY 6 cut(s) 58, 98, 157, 315, 321, 387
CviKI_1 RGCY 6 cut(s) 58, 98, 157, 315, 321, 387
CviQI GTAC 2 cut(s) 274, 374
DdeI CTNAG 3 cut(s) 308, 383, 482
EaeI YGGCCR 1 cut(s) 313
EagI CGGCCG 1 cut(s) 313
EclXI CGGCCG 1 cut(s) 313
Eco24I GRGCYC 1 cut(s) 100
Eco47I GGWCC 1 cut(s) 207
Eco52I CGGCCG 1 cut(s) 313
EcoO109I RGGNCCY 1 cut(s) 97
EcoRII CCWGG 2 cut(s) 50, 388
EcoT38I GRGCYC 1 cut(s) 100
FaeI CATG 1 cut(s) 41
FaiI YATR 9 cut(s) 39, 69, 144, 180, 242, 244, 298, 409, 443
FalI AAGNNNNNCTT 2 cut(s) 76, 108
FatI CATG 1 cut(s) 37
FauI CCCGC 1 cut(s) 254
Fnu4HI GCNGC 1 cut(s) 313
FokI GGATG 2 cut(s) 349, 390
FriOI GRGCYC 1 cut(s) 100
Fsp4HI GCNGC 1 cut(s) 313
FspBI CTAG 2 cut(s) 5, 461
GluI GCNGC 1 cut(s) 313
HaeIII GGCC 2 cut(s) 98, 315
Hin1II CATG 1 cut(s) 41
HinfI GANTC 1 cut(s) 47
HphI GGTGA 1 cut(s) 371
Hpy166II GTNNAC 3 cut(s) 90, 202, 274
Hpy188I TCNGA 1 cut(s) 309
Hpy8I GTNNAC 3 cut(s) 90, 202, 274
HpyAV CCTTC 2 cut(s) 74, 78
HpyCH4V TGCA 1 cut(s) 41
HpyF10VI GCNNNNNNNGC 1 cut(s) 318
HpyF3I CTNAG 3 cut(s) 308, 383, 482
Hsp92II CATG 1 cut(s) 41
LmnI GCTCC 1 cut(s) 55
LpnPI CCDG 8 cut(s) 37, 64, 72, 190, 223, 375, 402, 427
MaeI CTAG 2 cut(s) 5, 461
MaeIII GTNAC 2 cut(s) 212, 250
MbiI CCGCTC 1 cut(s) 312
MhlI GDGCHC 1 cut(s) 100
MluCI AATT 2 cut(s) 220, 467
MmeI TCCRAC 1 cut(s) 55
MnlI CCTC 6 cut(s) 71, 190, 251, 280, 311, 392
MroXI GAANNNNTTC 2 cut(s) 224, 468
MseI TTAA 1 cut(s) 108
MslI CAYNNNNRTG 1 cut(s) 141
MspR9I CCNGG 2 cut(s) 52, 390
MvaI CCWGG 2 cut(s) 52, 390
MwoI GCNNNNNNNGC 1 cut(s) 318
NlaIII CATG 1 cut(s) 41
NlaIV GGNNCC 3 cut(s) 57, 98, 99
NmeAIII GCCGAG 1 cut(s) 341
NspI RCATGY 1 cut(s) 41
PaeI GCATGC 1 cut(s) 41
PcsI WCGNNNNNNNCGW 1 cut(s) 262
PdmI GAANNNNTTC 2 cut(s) 224, 468
PfeI GAWTC 1 cut(s) 47
PkrI GCNGC 1 cut(s) 314
Psp6I CCWGG 2 cut(s) 50, 388
PspGI CCWGG 2 cut(s) 50, 388
PspN4I GGNNCC 3 cut(s) 57, 98, 99
PspOMI GGGCCC 1 cut(s) 96
PspPI GGNCC 3 cut(s) 96, 97, 207
RsaI GTAC 2 cut(s) 275, 375
RsaNI GTAC 2 cut(s) 274, 374
RseI CAYNNNNRTG 1 cut(s) 141
SaqAI TTAA 1 cut(s) 108
SatI GCNGC 1 cut(s) 313
Sau96I GGNCC 3 cut(s) 96, 97, 207
ScrFI CCNGG 2 cut(s) 52, 390
SduI GDGCHC 1 cut(s) 100
SetI ASST 3 cut(s) 89, 201, 384
SinI GGWCC 1 cut(s) 207
SmiMI CAYNNNNRTG 1 cut(s) 141
SmlI CTYRAG 1 cut(s) 194
SmoI CTYRAG 1 cut(s) 194
SphI GCATGC 1 cut(s) 41
Sse9I AATT 2 cut(s) 220, 467
SsiI CCGC 2 cut(s) 261, 312
SspMI CTAG 2 cut(s) 5, 461
StyD4I CCNGG 2 cut(s) 50, 388
TaqI TCGA 1 cut(s) 228
TasI AATT 2 cut(s) 220, 467
TatI WGTACW 1 cut(s) 273
TauI GCSGC 1 cut(s) 315
TfiI GAWTC 1 cut(s) 47
Tru1I TTAA 1 cut(s) 108
Tru9I TTAA 1 cut(s) 108
TspDTI ATGAA 2 cut(s) 56, 353
VpaK11BI GGWCC 1 cut(s) 207
XapI RAATTY 1 cut(s) 467
XceI RCATGY 1 cut(s) 41
XmnI GAANNNNTTC 2 cut(s) 224, 468
XspI CTAG 2 cut(s) 5, 461
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.