Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0001963

Physical Location & Seq

Genomic Coordinates Forward (+)

11545656 .. 11548293

Gene Structure 2638 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT2G27050.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1755 bp

ATGATGATGTTTAACGAGATGGGAATGTATGGAAACATGGATTTCTTCTCTTCCTCCACATCTCTCGATGTGTGTCCATTACCACAAGCTGAACAAGAACCTGTAGTTGAAGATGTCGACTACACCGATGATGAGATGGATGTGGATGAGCTTGAGAAGAGGATGTGGAGAGACAAAATGCGTTTGAAACGTCTCAAGGAGCAACAGAGTAAGTGTAAAGAAGGCGTCGATGGTTCGAAACAGAGGCAGTCGCAAGAGCAAGCTAGGAGGAAGAAAATGTCTAGAGCCCAAGATGGGATCTTGAAGTATATGTTGAAGATGATGGAAGTTTGTAAAGCTCAAGGCTTTGTTTATGGTATTATTCCTGAGAAGGGTAAGCCTGTGACTGGTGCTTCGGATAATTTGAGGGAATGGTGGAAAGATAAGGTTAGGTTTGATCGTAATGGTCCAGCTGCTATTGCTAAGTATCAGTCAGAGAATAATATTTCTGGAGGGAGTAATGATTGTAACAGCTTGGTTGGTCCAACACCGCATACGCTTCAGGAGCTTCAGGACACGACTCTTGGTTCGCTTTTATCGGCTTTGATGCAACATTGTGATCCACCGCAGAGACGGTTTCCTTTGGAGAAAGGAGTTTCTCCACCTTGGTGGCCTAATGGGAATGAAGAGTGGTGGCCTCAGCTTGGTTTACCAAATGAGCAAGGTCCTCCTCCTTATAAGAAGCCTCATGATTTGAAGAAAGCTTGGAAAGTCGGTGTTTTAACTGCGGTGATCAAGCATATGTCGCCGGATATTGCGAAGATCCGTAAGCTTGTGAGGCAATCAAAATGCTTGCAGGATAAGATGACGGCGAAAGAGAGTGCTACTTGGCTTGCCATTATTAACCAAGAAGAGGTTGTGGCTCGGGAGCTTTATCCCGAGTCATGCCCTCCTCTTTCTTCTTCTTCATCATTAGGAAGCGGGTCGCTTCTCATTAATGATTGTAGCGAGTATGACGTTGAAGGTTTCGAGAAGGAACAACATGGTTTCGATGTGGAAGAGCGGAAACCAGAGATAGTGATGATGCATCCTCTAGCAAGCTTTGGGGTTGCTAAAATGCAACATTTTCCCATAAAGGAGGAGGTCGCCACCACGGTAAACTTAGAGTTCACGAGAAAGAGGAAGCAGAACAATGATATGAATGTTATGGTAATGGACAGATCAGCAGGTTACACTTGTGAGAATGGTCAGTGTCCTCACAGCAAAATGAATCTTGGATTTCAAGACAGGAGTTCAAGGGACAACCACCAGATGGTTTGTCCATATAGAGACAATCGTTTAGCGTATGGAGCATCCAAGTTTCATATGGGTGGAATGAAACTAGTAGTTCCTCAGCAACCAGTCCAACCGATCGACCTATCGGGCGTTGGAGTTCCGGAAAACGGGCAGAAGATGATCACCGAGCTTATGGCCATGTACGACAGAAATGTCCAAAGCAACCAAACGCCTCCTACTTTGATGGAAAACCAAAGCATGGTCATTGATGCAAAAGCAGCTCAGAATCAGCAGCTGAATTTCAACAGTGGCAATCAAATGTTTATGCAACAAGGGACGAACAACGGGGTTAACAATCGGTTCCAGATGGTGTTTGATTCGACACCATTCGATATGGCAGCATTCGATTACAGAGATGATTGGCAAACCGGAGCAATGGAAGGAATGGGGAAGCAGCAGCAGCAGCAGCAGCAGCAGCAAGATGTATCAATATGGTTCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000160 GO:0000976 GO:0001067 GO:0001666 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0006950 GO:0006952 GO:0007154 GO:0007165 GO:0008150 GO:0009605 GO:0009607 GO:0009617 GO:0009628 GO:0009719 GO:0009723 GO:0009725 GO:0009743 GO:0009755 GO:0009756 GO:0009873 GO:0009889 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010039 GO:0010182 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0023052 GO:0031323 GO:0031326 GO:0032870 GO:0035556 GO:0036293 GO:0042221 GO:0042742 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044212 GO:0044424 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051252 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0065007 GO:0070482 GO:0070887 GO:0071241 GO:0071248 GO:0071281 GO:0071310 GO:0071322 GO:0071369 GO:0071495 GO:0080090 GO:0097159 GO:0098542 GO:0140110 GO:1901363 GO:1901700 GO:1901701 GO:1903506 GO:1990837 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

ko04016 ko04075 map04016 map04075

Pfam Domains

Protein Families

EIN3

Protein Analysis

584

Amino Acids

66.49

Weight (kDa)

5.83

Isoelectric Point (pI)

52.4

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
EIN3_DNA-bd	PF04873	49 - 298	1.1e-131	Ethylene insensitive 3, DNA-binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0001963)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G27050 AT3G20770
fragaria_vesca	FvH4_1g28720 FvH4_1g28720 FvH4_7g04940 FvH4_7g04940 FvH4_7g04940 FvH4_7g04940 FvH4_7g04940
malus_domestica	MD02G1266200.v1.1 MD02G1266300.v1.1 MD07G1053500.v1.1 MD07G1053800.v1.1 MD08G1245800.v1.1 MD15G1441000.v1.1
prunus_persica	Prupe.2G058400_v2.0.a1 Prupe.2G058500_v2.0.a1 Prupe.2G070300_v2.0.a1
pyrus_communis	pycom02g22750 pycom02g22760 pycom07g04140 pycom07g04160 pycom08g21330 pycom15g38880
rosa_chinensis	RchiOBHm_Chr1g0331011 RchiOBHm_Chr5g0049961
rosa_laevigata	RLG00000029759 RLG00000034672
rosa_multiflora	Rmu_sc0000333.1_g000038 Rmu_sc0013622.1_g000002 Rmu_sc0013622.1_g000003
rosa_roxburghii	Rroxscaffold_1G00031290 Rroxscaffold_4G00319550
rosa_rugosa	Rorug01G0091600 Rorug05G0255200
rosa_samantha	Rh1AG113300 Rh1BG087200 Rh1CG110200 Rh1DG118900 Rh5AG329100 Rh5BG339600 Rh5CG365600 Rh5DG352200
rosa_wichuraiana	Rw1G009240 Rw5G031070

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	717
AasI	GACNNNNNNGTC	1 cut(s)	1466
Acc36I	ACCTGC	1 cut(s)	1196
AccB7I	CCANNNNNTGG	2 cut(s)	1291, 1513
AccBSI	CCGCTC	1 cut(s)	1042
AccI	GTMKAC	1 cut(s)	117
AccIII	TCCGGA	1 cut(s)	1414
AciI	CCGC	5 cut(s)	530, 605, 767, 960, 1042
AclWI	GGATC	3 cut(s)	305, 593, 796
AcoI	YGGCCR	1 cut(s)	1449
AcsI	RAATTY	1 cut(s)	1552
AcuI	CTGAAG	2 cut(s)	524, 533
AcyI	GRCGYC	1 cut(s)	225
AfaI	GTAC	1 cut(s)	1457
AfiI	CCNNNNNNNGG	8 cut(s)	294, 371, 386, 683, 892, 1291, 1421, 1513
AgsI	TTSAA	9 cut(s)	110, 187, 304, 316, 736, 1001, 1262, 1275, 1558
AhlI	ACTAGT	1 cut(s)	1360
AleI	CACNNNNGTG	1 cut(s)	646
Alw26I	GTCTC	4 cut(s)	165, 197, 604, 1302
AlwI	GGATC	3 cut(s)	305, 593, 796
AlwNI	CAGNNNCTG	1 cut(s)	1549
Ama87I	CYCGRG	2 cut(s)	903, 917
Aor13HI	TCCGGA	1 cut(s)	1414
AoxI	GGCC	3 cut(s)	650, 674, 1449
ApoI	RAATTY	1 cut(s)	1552
ArsI	GACNNNNNNTTYG	4 cut(s)	376, 408, 550, 582
AseI	ATTAAT	1 cut(s)	975
AspS9I	GGNCC	3 cut(s)	446, 521, 704
AsuHPI	GGTGA	2 cut(s)	781, 1429
AsuII	TTCGAA	1 cut(s)	236
AvaI	CYCGRG	2 cut(s)	903, 917
AvaII	GGWCC	3 cut(s)	446, 521, 704
BalI	TGGCCA	1 cut(s)	1451
BanII	GRGCYC	1 cut(s)	289
BauI	CACGAG	1 cut(s)	1150
BbvCI	CCTCAGC	2 cut(s)	678, 1371
BccI	CCATC	8 cut(s)	13, 130, 224, 287, 316, 1285, 1492, 1615
BceAI	ACGGC	1 cut(s)	864
BclI	TGATCA	2 cut(s)	771, 1434
BcoDI	GTCTC	4 cut(s)	165, 197, 604, 1302
BcuI	ACTAGT	1 cut(s)	1360
BfaI	CTAG	4 cut(s)	264, 282, 1073, 1361
BfmI	CTRYAG	1 cut(s)	102
BfuAI	ACCTGC	1 cut(s)	1196
Bme18I	GGWCC	3 cut(s)	446, 521, 704
BmeT110I	CYCGRG	2 cut(s)	903, 917
BmgT120I	GGNCC	3 cut(s)	446, 521, 704
BmiI	GGNNCC	1 cut(s)	1616
BmsI	GCATC	5 cut(s)	576, 1053, 1075, 1340, 1513
BpmI	CTGGAG	1 cut(s)	510
Bpu10I	CCTNAGC	2 cut(s)	678, 1371
Bpu14I	TTCGAA	1 cut(s)	236
BpuEI	CTTGAG	3 cut(s)	173, 179, 324
BsaHI	GRCGYC	1 cut(s)	225
BsaJI	CCNNGG	2 cut(s)	644, 1131
BsaWI	WCCGGW	2 cut(s)	1414, 1682
Bsc4I	CCNNNNNNNGG	8 cut(s)	294, 371, 386, 683, 892, 1291, 1421, 1513
Bse1I	ACTGG	2 cut(s)	391, 1379
Bse3DI	GCAATG	1 cut(s)	1695
BseAI	TCCGGA	1 cut(s)	1414
BseDI	CCNNGG	2 cut(s)	644, 1131
BseGI	GGATG	5 cut(s)	145, 151, 168, 1066, 1331
BseLI	CCNNNNNNNGG	8 cut(s)	294, 371, 386, 683, 892, 1291, 1421, 1513
BseMI	GCAATG	1 cut(s)	1695
BseMII	CTCAG	4 cut(s)	357, 692, 1385, 1550
BseNI	ACTGG	2 cut(s)	391, 1379
BseRI	GAGGAG	3 cut(s)	699, 921, 1133
Bsh1285I	CGRYCG	1 cut(s)	1392
BshFI	GGCC	3 cut(s)	652, 676, 1451
BsiEI	CGRYCG	1 cut(s)	1392
BsiHKCI	CYCGRG	2 cut(s)	903, 917
BsiSI	CCGG	3 cut(s)	788, 1415, 1683
BslFI	GGGAC	2 cut(s)	1292, 1603
BslI	CCNNNNNNNGG	8 cut(s)	294, 371, 386, 683, 892, 1291, 1421, 1513
BsmAI	GTCTC	4 cut(s)	165, 197, 604, 1302
BsmBI	CGTCTC	2 cut(s)	197, 604
BsmFI	GGGAC	2 cut(s)	1292, 1603
BsmI	GAATGC	1 cut(s)	1655
BsnI	GGCC	3 cut(s)	652, 676, 1451
BsoBI	CYCGRG	2 cut(s)	903, 917
Bsp119I	TTCGAA	1 cut(s)	236
Bsp1286I	GDGCHC	1 cut(s)	289
Bsp13I	TCCGGA	1 cut(s)	1414
Bsp143I	GATC	8 cut(s)	297, 436, 598, 771, 801, 1199, 1389, 1434
BspACI	CCGC	5 cut(s)	530, 605, 767, 960, 1042
BspANI	GGCC	3 cut(s)	652, 676, 1451
BspCNI	CTCAG	4 cut(s)	358, 691, 1384, 1549
BspEI	TCCGGA	1 cut(s)	1414
BspHI	TCATGA	1 cut(s)	727
BspLI	GGNNCC	1 cut(s)	1616
BspMI	ACCTGC	1 cut(s)	1196
BspPI	GGATC	3 cut(s)	305, 593, 796
BspQI	GCTCTTC	1 cut(s)	1032
BspT104I	TTCGAA	1 cut(s)	236
BsrBI	CCGCTC	1 cut(s)	1042
BsrDI	GCAATG	1 cut(s)	1695
BsrI	ACTGG	2 cut(s)	391, 1379
BssECI	CCNNGG	2 cut(s)	644, 1131
BssMI	GATC	8 cut(s)	297, 436, 598, 771, 801, 1199, 1389, 1434
BssNI	GRCGYC	1 cut(s)	225
BssSI	CACGAG	1 cut(s)	1150
BssT1I	CCWWGG	1 cut(s)	644
Bst2BI	CACGAG	1 cut(s)	1150
Bst4CI	ACNGT	3 cut(s)	615, 1135, 1562
Bst6I	CTCTTC	5 cut(s)	55, 152, 660, 885, 1032
BstACI	GRCGYC	1 cut(s)	225
BstBI	TTCGAA	1 cut(s)	236
BstC8I	GCNNGC	4 cut(s)	261, 833, 873, 1078
BstDEI	CTNAG	6 cut(s)	366, 462, 678, 1141, 1371, 1536
BstDSI	CCRYGG	1 cut(s)	1131
BstF5I	GGATG	5 cut(s)	145, 151, 168, 1066, 1331
BstKTI	GATC	8 cut(s)	300, 439, 601, 774, 804, 1202, 1392, 1437
BstMAI	GTCTC	4 cut(s)	165, 197, 604, 1302
BstMBI	GATC	8 cut(s)	297, 436, 598, 771, 801, 1199, 1389, 1434
BstMCI	CGRYCG	1 cut(s)	1392
BstSFI	CTRYAG	1 cut(s)	102
BstX2I	RGATCY	2 cut(s)	297, 801
BstXI	CCANNNNNNTGG	1 cut(s)	648
BstYI	RGATCY	2 cut(s)	297, 801
BsuRI	GGCC	3 cut(s)	652, 676, 1451
BtgI	CCRYGG	1 cut(s)	1131
BtsCI	GGATG	5 cut(s)	145, 151, 168, 1066, 1331
BtsIMutI	CAGTG	2 cut(s)	1235, 1567
BveI	ACCTGC	1 cut(s)	1196
Cac8I	GCNNGC	4 cut(s)	261, 833, 873, 1078
CaiI	CAGNNNCTG	1 cut(s)	1549
CciI	TCATGA	1 cut(s)	727
Cfr13I	GGNCC	3 cut(s)	446, 521, 704
CseI	GACGC	1 cut(s)	214
Csp6I	GTAC	1 cut(s)	1456
CviAII	CATG	6 cut(s)	37, 728, 924, 1022, 1453, 1513
CviQI	GTAC	1 cut(s)	1456
DdeI	CTNAG	6 cut(s)	366, 462, 678, 1141, 1371, 1536
DpnI	GATC	8 cut(s)	299, 438, 600, 773, 803, 1201, 1391, 1436
DpnII	GATC	8 cut(s)	297, 436, 598, 771, 801, 1199, 1389, 1434
DrdI	GACNNNNNNGTC	1 cut(s)	1466
DseDI	GACNNNNNNGTC	1 cut(s)	1466
EaeI	YGGCCR	1 cut(s)	1449
Eam1104I	CTCTTC	5 cut(s)	55, 152, 660, 885, 1032
EarI	CTCTTC	5 cut(s)	55, 152, 660, 885, 1032
Eco130I	CCWWGG	1 cut(s)	644
Eco24I	GRGCYC	1 cut(s)	289
Eco47I	GGWCC	3 cut(s)	446, 521, 704
Eco57I	CTGAAG	2 cut(s)	524, 533
Eco88I	CYCGRG	2 cut(s)	903, 917
EcoO109I	RGGNCCY	1 cut(s)	704
EcoT14I	CCWWGG	1 cut(s)	644
EcoT22I	ATGCAT	1 cut(s)	1068
EcoT38I	GRGCYC	1 cut(s)	289
ErhI	CCWWGG	1 cut(s)	644
Esp3I	CGTCTC	2 cut(s)	197, 604
FaeI	CATG	6 cut(s)	40, 731, 927, 1025, 1456, 1516
FaqI	GGGAC	2 cut(s)	1292, 1603
FatI	CATG	6 cut(s)	36, 727, 923, 1021, 1452, 1512
FauI	CCCGC	1 cut(s)	953
FauNDI	CATATG	2 cut(s)	780, 1344
FbaI	TGATCA	2 cut(s)	771, 1434
FblI	GTMKAC	1 cut(s)	117
FokI	GGATG	5 cut(s)	152, 158, 175, 1053, 1318
FriOI	GRGCYC	1 cut(s)	289
FspBI	CTAG	4 cut(s)	264, 282, 1073, 1361
GsuI	CTGGAG	1 cut(s)	510
HaeIII	GGCC	3 cut(s)	652, 676, 1451
HapII	CCGG	3 cut(s)	788, 1415, 1683
HgaI	GACGC	1 cut(s)	214
Hin1I	GRCGYC	1 cut(s)	225
Hin1II	CATG	6 cut(s)	40, 731, 927, 1025, 1456, 1516
HincII	GTYRAC	2 cut(s)	118, 1606
HindII	GTYRAC	2 cut(s)	118, 1606
HindIII	AAGCTT	3 cut(s)	741, 809, 1078
HinfI	GANTC	5 cut(s)	559, 920, 1249, 1540, 1631
HpaI	GTTAAC	1 cut(s)	1606
HpaII	CCGG	3 cut(s)	788, 1415, 1683
HphI	GGTGA	2 cut(s)	781, 1429
Hpy166II	GTNNAC	5 cut(s)	118, 689, 1138, 1149, 1606
Hpy188I	TCNGA	4 cut(s)	397, 475, 1539, 1754
Hpy8I	GTNNAC	5 cut(s)	118, 689, 1138, 1149, 1606
Hpy99I	CGWCG	1 cut(s)	230
HpyAV	CCTTC	5 cut(s)	215, 364, 995, 1006, 1688
HpyCH4III	ACNGT	3 cut(s)	615, 1135, 1562
HpyCH4IV	ACGT	2 cut(s)	190, 996
HpyCH4V	TGCA	6 cut(s)	589, 835, 1066, 1099, 1526, 1582
HpyF3I	CTNAG	6 cut(s)	366, 462, 678, 1141, 1371, 1536
HpySE526I	ACGT	2 cut(s)	190, 996
Hsp92I	GRCGYC	1 cut(s)	225
Hsp92II	CATG	6 cut(s)	40, 731, 927, 1025, 1456, 1516
Kpn2I	TCCGGA	1 cut(s)	1414
Ksp22I	TGATCA	2 cut(s)	771, 1434
KspAI	GTTAAC	1 cut(s)	1606
Kzo9I	GATC	8 cut(s)	297, 436, 598, 771, 801, 1199, 1389, 1434
LguI	GCTCTTC	1 cut(s)	1032
LmnI	GCTCC	5 cut(s)	199, 544, 907, 1328, 1685
LweI	GCATC	5 cut(s)	576, 1053, 1075, 1340, 1513
MaeI	CTAG	4 cut(s)	264, 282, 1073, 1361
MaeII	ACGT	2 cut(s)	190, 996
MaeIII	GTNAC	3 cut(s)	382, 506, 1208
MalI	GATC	8 cut(s)	299, 438, 600, 773, 803, 1201, 1391, 1436
MbiI	CCGCTC	1 cut(s)	1042
MboI	GATC	8 cut(s)	297, 436, 598, 771, 801, 1199, 1389, 1434
MflI	RGATCY	2 cut(s)	297, 801
MhlI	GDGCHC	1 cut(s)	289
MlsI	TGGCCA	1 cut(s)	1451
MluCI	AATT	2 cut(s)	400, 1552
MluNI	TGGCCA	1 cut(s)	1451
MlyI	GAGTC	2 cut(s)	553, 929
MmeI	TCCRAC	3 cut(s)	548, 1387, 1408
Mox20I	TGGCCA	1 cut(s)	1451
Mph1103I	ATGCAT	1 cut(s)	1068
MroI	TCCGGA	1 cut(s)	1414
MscI	TGGCCA	1 cut(s)	1451
MseI	TTAA	5 cut(s)	12, 761, 882, 975, 1605
MslI	CAYNNNNRTG	2 cut(s)	646, 1347
Msp20I	TGGCCA	1 cut(s)	1451
MspA1I	CMGCKG	2 cut(s)	452, 1549
MspI	CCGG	3 cut(s)	788, 1415, 1683
Mva1269I	GAATGC	1 cut(s)	1655
NdeI	CATATG	2 cut(s)	780, 1344
NdeII	GATC	8 cut(s)	297, 436, 598, 771, 801, 1199, 1389, 1434
NlaIII	CATG	6 cut(s)	40, 731, 927, 1025, 1456, 1516
NlaIV	GGNNCC	1 cut(s)	1616
NmuCI	GTSAC	1 cut(s)	382
NsiI	ATGCAT	1 cut(s)	1068
NspV	TTCGAA	1 cut(s)	236
OliI	CACNNNNGTG	1 cut(s)	646
PagI	TCATGA	1 cut(s)	727
PciSI	GCTCTTC	1 cut(s)	1032
PcsI	WCGNNNNNNNCGW	1 cut(s)	123
PctI	GAATGC	1 cut(s)	1655
PfeI	GAWTC	3 cut(s)	1249, 1540, 1631
PflMI	CCANNNNNTGG	2 cut(s)	1291, 1513
Ple19I	CGATCG	1 cut(s)	1392
PleI	GAGTC	2 cut(s)	553, 928
PpsI	GAGTC	2 cut(s)	553, 928
PpuMI	RGGWCCY	1 cut(s)	704
PshBI	ATTAAT	1 cut(s)	975
PsiI	TTATAA	1 cut(s)	717
Psp5II	RGGWCCY	1 cut(s)	704
PspN4I	GGNNCC	1 cut(s)	1616
PspPI	GGNCC	3 cut(s)	446, 521, 704
PspPPI	RGGWCCY	1 cut(s)	704
PstNI	CAGNNNCTG	1 cut(s)	1549
PsuI	RGATCY	2 cut(s)	297, 801
PvuI	CGATCG	1 cut(s)	1392
PvuII	CAGCTG	2 cut(s)	452, 1549
RsaI	GTAC	1 cut(s)	1457
RsaNI	GTAC	1 cut(s)	1456
RseI	CAYNNNNRTG	2 cut(s)	646, 1347
SalI	GTCGAC	1 cut(s)	116
SapI	GCTCTTC	1 cut(s)	1032
SaqAI	TTAA	5 cut(s)	12, 761, 882, 975, 1605
Sau3AI	GATC	8 cut(s)	297, 436, 598, 771, 801, 1199, 1389, 1434
Sau96I	GGNCC	3 cut(s)	446, 521, 704
SchI	GAGTC	2 cut(s)	553, 929
SduI	GDGCHC	1 cut(s)	289
SfaNI	GCATC	5 cut(s)	576, 1053, 1075, 1340, 1513
SfcI	CTRYAG	1 cut(s)	102
SfuI	TTCGAA	1 cut(s)	236
SinI	GGWCC	3 cut(s)	446, 521, 704
SmiMI	CAYNNNNRTG	2 cut(s)	646, 1347
SmlI	CTYRAG	3 cut(s)	152, 194, 339
SmoI	CTYRAG	3 cut(s)	152, 194, 339
SpeI	ACTAGT	1 cut(s)	1360
Sse9I	AATT	2 cut(s)	400, 1552
SsiI	CCGC	5 cut(s)	530, 605, 767, 960, 1042
SspI	AATATT	1 cut(s)	484
SspMI	CTAG	4 cut(s)	264, 282, 1073, 1361
StyI	CCWWGG	1 cut(s)	644
TaaI	ACNGT	3 cut(s)	615, 1135, 1562
TaiI	ACGT	2 cut(s)	193, 999
TasI	AATT	2 cut(s)	400, 1552
TfiI	GAWTC	3 cut(s)	1249, 1540, 1631
Tru1I	TTAA	5 cut(s)	12, 761, 882, 975, 1605
Tru9I	TTAA	5 cut(s)	12, 761, 882, 975, 1605
TscAI	CASTG	2 cut(s)	1235, 1567
TseFI	GTSAC	1 cut(s)	382
Tsp45I	GTSAC	1 cut(s)	382
TspDTI	ATGAA	6 cut(s)	678, 936, 1193, 1262, 1331, 1370
TspGWI	ACGGA	1 cut(s)	794
TspRI	CASTG	2 cut(s)	1235, 1567
Van91I	CCANNNNNTGG	2 cut(s)	1291, 1513
VpaK11BI	GGWCC	3 cut(s)	446, 521, 704
VspI	ATTAAT	1 cut(s)	975
XapI	RAATTY	1 cut(s)	1552
XbaI	TCTAGA	1 cut(s)	281
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1342
XmiI	GTMKAC	1 cut(s)	117
XspI	CTAG	4 cut(s)	264, 282, 1073, 1361
Zsp2I	ATGCAT	1 cut(s)	1068

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.