Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0001963

Physical Location & Seq

Genomic Coordinates Reverse (-)

31027941 .. 31031302

Gene Structure 3362 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1245800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1878 bp

ATGGGGATCTTTGAGGAGTTGGGCTTCTGTGATAATCTTGACTTTCTTTCTGCTCCATCTGAGGAAGGGGATGCAGCTCCAGAGCATGAACCAGAGGCGACAGCAGAGGAGGATTATAGTGATGAAGAAATGGATGTTGATGAGCTCGAGAAGAGGATGTGGAGAGATAGAATGCTATTGAAGAGGCTGAAAGAACAAACTAAGGGAAAGGAAGGAGTTGACAATGCAAGACAGCGTCAATCACAGGAACAAGCTCGGAGGAAGAAGATGTCACGGGCACAAGATGGAATCCTGAAATATATGCTGAAAATGATGGAAGTTTGCAAAGCTCAAGGTTTTGTTTATGGAATTATCCCTGAAAAAGGAAAACCAGTGAGTGGCGCTTCTGACAATCTGAGAGCATGGTGGAAGGAAAAAGTAAGATTTGATCGTAATGGCCCAGCTGCCATTTCTAAGTATCAGGCGGATCATTCAATCCCTGGGAAGAATGAACACTTCAGTGCGGTGGCCTCCACTCCTCACACCTTGCAGGAGCTCCAAGACACCACTCTTGGTTCACTTTTGTCAGCTTTGATGCAGCACTGCAATCCACCCCAAAGACGTTTCCCGTTGGAGAAGGGTGTTGCCCCACCTTGGTGGCCTACTGGTAATGAGGAATGGTGGCCTCAACTAAATCTGCCCAAGGATCAGGGTCCTCCCCCATACAAGAAGCCTCATGATCTGAAGAAGGCTTGGAAGGTTGGTGTTCTCACGGCTGTGATAAAGCACATGTCCCCAGATATTGCCAAGATCCGCAAGCTTGTTTGTCAGTCTAAATGTTTGCAGGACAAAATGACTGCCAAGGAGAGTGCCACTTGGCTAGCCATTTTAAACCAGGAGGAAGCTTTGGCCCGGAGGCTATATCCTGATAGATGTCCACCTCCATTTGCTGGTGGGAATGACTCTCTAGCAATCAGTGGAACTAGTGATTATGATGTCGAAGGGGTTGATGATGACGAAAATGTTGAAATAGAGGATTGCAAACCTCTTGTTAATCACTTCAACATTGGAGCCACTGGACAAAGAGAGAGGCTGGGACCTCAGATTAAGAGAGAGCTTATTGAGATCAACTCCGATTTTGGTCAAAAGAGGAAGCAGCTGGCTGAAGAGCCACAAATGATGTTAAATCAGAAGGTTTACACCTGCGAATATCTGCAGTGCCCGTATCATGATTATCGCCTAGGCTTTCTTGACATAACTGCAAGAAACAATCATCAGTTGAATTGTCCACACCGGAGTAATTCTTCACAAGTGCTTGGAATGTCAAGCTTTCAGCTTCACAATGAGACACCTGTAAGCTTCTCTCTACCCATTGCTCAACAACCTACACCAGCAAATCAACCAGTGAACCAGTCAAGTATGTTTGATGATTCGGGACTTGGAATTCAACAACCAGTGAACCAGTCACGTAGGTTTGATGCTTCAGGACTTGGAGTTGCTGAGGATGGGCAGAAAATGATATCGGATCTTATGTCATTCTATGATAGTAATATTCAGCAGAACAAGAACTGCAATCCTGGAAACCTCAATGTTATAGATGATCGCAATCAGCAGCAGGCGAACTATCAATTTCCAATGAATGATAACTTGTTTGGTCACGGCGTGGACATAGGACGCAACATGAACATGTCGGAACCATCCCCCATGCTTATGCTTCATCCAGGCTTTTCATCCCCGGAAGTTCAGTTTGATCAGTTGATGGCATTTGAGTCTCCATTTGGAAACAATTCCAGCGAAGATGTCGACATAAGATTTGACTCCCCCTTTCACTTGGCGCACGTTGGTTATAATGCTATGGACCCGCCAGTGAACCAAGATGCATCCCCCTGGTTCCCATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000160 GO:0000976 GO:0001067 GO:0001666 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0006950 GO:0006952 GO:0007154 GO:0007165 GO:0008150 GO:0009605 GO:0009607 GO:0009617 GO:0009628 GO:0009719 GO:0009723 GO:0009725 GO:0009743 GO:0009755 GO:0009756 GO:0009873 GO:0009889 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010039 GO:0010182 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0023052 GO:0031323 GO:0031326 GO:0032870 GO:0035556 GO:0036293 GO:0042221 GO:0042742 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044212 GO:0044424 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051252 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0065007 GO:0070482 GO:0070887 GO:0071241 GO:0071248 GO:0071281 GO:0071310 GO:0071322 GO:0071369 GO:0071495 GO:0080090 GO:0097159 GO:0098542 GO:0140110 GO:1901363 GO:1901700 GO:1901701 GO:1903506 GO:1990837 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

ko04016 ko04075 map04016 map04075

Pfam Domains

Protein Families

EIN3

Protein Analysis

626

Amino Acids

70.65

Weight (kDa)

5.25

Isoelectric Point (pI)

58.29

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
EIN3_DNA-bd	PF04873	47 - 294	1.3e-131	Ethylene insensitive 3, DNA-binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001963)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G27050 AT3G20770
fragaria_vesca	FvH4_1g28720 FvH4_1g28720 FvH4_7g04940 FvH4_7g04940 FvH4_7g04940 FvH4_7g04940 FvH4_7g04940
malus_domestica	MD02G1266200.v1.1 MD02G1266300.v1.1 MD07G1053500.v1.1 MD07G1053800.v1.1 MD08G1245800.v1.1 MD15G1441000.v1.1
prunus_persica	Prupe.2G058400_v2.0.a1 Prupe.2G058500_v2.0.a1 Prupe.2G070300_v2.0.a1
pyrus_communis	pycom02g22750 pycom02g22760 pycom07g04140 pycom07g04160 pycom08g21330 pycom15g38880
rosa_chinensis	RchiOBHm_Chr1g0331011 RchiOBHm_Chr5g0049961
rosa_laevigata	RLG00000029759 RLG00000034672
rosa_multiflora	Rmu_sc0000333.1_g000038 Rmu_sc0013622.1_g000002 Rmu_sc0013622.1_g000003
rosa_roxburghii	Rroxscaffold_1G00031290 Rroxscaffold_4G00319550
rosa_rugosa	Rorug01G0091600 Rorug05G0255200
rosa_samantha	Rh1AG113300 Rh1BG087200 Rh1CG110200 Rh1DG118900 Rh5AG329100 Rh5BG339600 Rh5CG365600 Rh5DG352200
rosa_wichuraiana	Rw1G009240 Rw5G031070

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1827
AarI	CACCTGC	1 cut(s)	1190
Acc36I	ACCTGC	1 cut(s)	1190
AccB7I	CCANNNNNTGG	2 cut(s)	377, 929
AccI	GTMKAC	1 cut(s)	1782
AciI	CCGC	4 cut(s)	464, 503, 793, 1841
AclWI	GGATC	5 cut(s)	14, 474, 693, 784, 1512
AcsI	RAATTY	1 cut(s)	1422
AcuI	CTGAAG	4 cut(s)	481, 743, 1164, 1446
AdeI	CACNNNGTG	1 cut(s)	1642
AfiI	CCNNNNNNNGG	4 cut(s)	362, 377, 633, 929
AflIII	ACRYGT	2 cut(s)	768, 1665
AgsI	TTSAA	6 cut(s)	181, 474, 1007, 1042, 1261, 1427
AhlI	ACTAGT	1 cut(s)	962
AjnI	CCWGG	5 cut(s)	478, 873, 1555, 1699, 1865
AleI	CACNNNNGTG	3 cut(s)	498, 634, 755
Alw21I	GWGCWC	2 cut(s)	147, 537
Alw26I	GTCTC	2 cut(s)	1319, 1755
AlwI	GGATC	5 cut(s)	14, 474, 693, 784, 1512
Ama87I	CYCGRG	1 cut(s)	146
AoxI	GGCC	5 cut(s)	436, 507, 638, 662, 888
ApeKI	GCWGC	5 cut(s)	74, 443, 577, 1135, 1591
ApoI	RAATTY	1 cut(s)	1422
ArsI	GACNNNNNNTTYG	2 cut(s)	1775, 1807
Asp700I	GAANNNNTTC	1 cut(s)	1765
AspA2I	CCTAGG	1 cut(s)	1219
AspLEI	GCGC	2 cut(s)	383, 1816
AspS9I	GGNCC	5 cut(s)	437, 692, 889, 1076, 1837
AsuC2I	CCSGG	2 cut(s)	892, 1715
AsuNHI	GCTAGC	1 cut(s)	859
AvaI	CYCGRG	1 cut(s)	146
AvaII	GGWCC	3 cut(s)	692, 1076, 1837
AvrII	CCTAGG	1 cut(s)	1219
BaeGI	GKGCMC	2 cut(s)	280, 1202
BanII	GRGCYC	2 cut(s)	147, 537
Bbv12I	GWGCWC	2 cut(s)	147, 537
BbvCI	CCTCAGC	1 cut(s)	1479
BbvI	GCAGC	5 cut(s)	86, 430, 589, 1147, 1603
BccI	CCATC	6 cut(s)	64, 278, 307, 1478, 1684, 1732
BceAI	ACGGC	2 cut(s)	768, 1654
BciT130I	CCWGG	5 cut(s)	480, 875, 1557, 1701, 1867
BclI	TGATCA	1 cut(s)	1729
BcnI	CCSGG	2 cut(s)	892, 1715
BcoDI	GTCTC	2 cut(s)	1319, 1755
BcuI	ACTAGT	1 cut(s)	962
BfaI	CTAG	4 cut(s)	860, 947, 963, 1220
BfmI	CTRYAG	1 cut(s)	1193
BfoI	RGCGCY	1 cut(s)	384
BfuAI	ACCTGC	1 cut(s)	1190
BisI	GCNGC	5 cut(s)	75, 444, 578, 1136, 1592
BlnI	CCTAGG	1 cut(s)	1219
BlsI	GCNGC	5 cut(s)	76, 445, 579, 1137, 1593
Bme1390I	CCNGG	7 cut(s)	480, 875, 892, 1557, 1701, 1715, 1867
Bme18I	GGWCC	3 cut(s)	692, 1076, 1837
BmeT110I	CYCGRG	1 cut(s)	146
BmgT120I	GGNCC	5 cut(s)	437, 692, 889, 1076, 1837
BmiI	GGNNCC	6 cut(s)	693, 1051, 1077, 1674, 1839, 1871
BmrFI	CCNGG	7 cut(s)	480, 875, 892, 1557, 1701, 1715, 1867
BmsI	GCATC	5 cut(s)	61, 564, 1447, 1846, 1868
BmtI	GCTAGC	1 cut(s)	863
BplI	GAGNNNNNCTC	2 cut(s)	1094, 1126
BpmI	CTGGAG	1 cut(s)	63
Bpu10I	CCTNAGC	1 cut(s)	1479
BpuEI	CTTGAG	1 cut(s)	315
BpuMI	CCSGG	2 cut(s)	892, 1715
BsaAI	YACGTR	1 cut(s)	1448
BsaBI	GATNNNNATC	1 cut(s)	1584
BsaJI	CCNNGG	8 cut(s)	478, 479, 632, 681, 840, 1219, 1713, 1865
BsaWI	WCCGGW	1 cut(s)	1272
Bsc4I	CCNNNNNNNGG	4 cut(s)	362, 377, 633, 929
Bse1I	ACTGG	8 cut(s)	371, 649, 1060, 1382, 1390, 1433, 1441, 1844
Bse3DI	GCAATG	1 cut(s)	1350
Bse8I	GATNNNNATC	1 cut(s)	1584
BseBI	CCWGG	5 cut(s)	480, 875, 1557, 1701, 1867
BseDI	CCNNGG	8 cut(s)	478, 479, 632, 681, 840, 1219, 1713, 1865
BseGI	GGATG	8 cut(s)	76, 139, 162, 1489, 1676, 1696, 1709, 1859
BseJI	GATNNNNATC	1 cut(s)	1584
BseLI	CCNNNNNNNGG	4 cut(s)	362, 377, 633, 929
BseMI	GCAATG	1 cut(s)	1350
BseMII	CTCAG	4 cut(s)	51, 386, 1094, 1470
BseNI	ACTGG	8 cut(s)	371, 649, 1060, 1382, 1390, 1433, 1441, 1844
BseRI	GAGGAG	3 cut(s)	29, 122, 507
BseSI	GKGCMC	2 cut(s)	280, 1202
BseXI	GCAGC	5 cut(s)	86, 430, 589, 1147, 1603
BseYI	CCCAGC	2 cut(s)	439, 1072
BshFI	GGCC	5 cut(s)	438, 509, 640, 664, 890
BsiHKAI	GWGCWC	2 cut(s)	147, 537
BsiHKCI	CYCGRG	1 cut(s)	146
BsiSI	CCGG	3 cut(s)	892, 1273, 1715
BslFI	GGGAC	3 cut(s)	757, 1089, 1428
BslI	CCNNNNNNNGG	4 cut(s)	362, 377, 633, 929
BsmAI	GTCTC	2 cut(s)	1319, 1755
BsmFI	GGGAC	3 cut(s)	757, 1089, 1428
BsmI	GAATGC	1 cut(s)	177
BsnI	GGCC	5 cut(s)	438, 509, 640, 664, 890
BsoBI	CYCGRG	1 cut(s)	146
Bsp1286I	GDGCHC	4 cut(s)	147, 280, 537, 1202
BspACI	CCGC	4 cut(s)	464, 503, 793, 1841
BspANI	GGCC	5 cut(s)	438, 509, 640, 664, 890
BspCNI	CTCAG	4 cut(s)	52, 387, 1093, 1471
BspHI	TCATGA	2 cut(s)	715, 1207
BspLI	GGNNCC	6 cut(s)	693, 1051, 1077, 1674, 1839, 1871
BspMAI	CTGCAG	1 cut(s)	1197
BspMI	ACCTGC	1 cut(s)	1190
BspOI	GCTAGC	1 cut(s)	863
BspPI	GGATC	5 cut(s)	14, 474, 693, 784, 1512
BspQI	GCTCTTC	1 cut(s)	1140
BsrDI	GCAATG	1 cut(s)	1350
BsrI	ACTGG	8 cut(s)	371, 649, 1060, 1382, 1390, 1433, 1441, 1844
BssECI	CCNNGG	8 cut(s)	478, 479, 632, 681, 840, 1219, 1713, 1865
BssT1I	CCWWGG	4 cut(s)	632, 681, 840, 1219
Bst2UI	CCWGG	5 cut(s)	480, 875, 1557, 1701, 1867
Bst6I	CTCTTC	3 cut(s)	146, 176, 1140
BstBAI	YACGTR	1 cut(s)	1448
BstC8I	GCNNGC	4 cut(s)	797, 861, 1140, 1596
BstDEI	CTNAG	6 cut(s)	60, 201, 395, 453, 1080, 1479
BstENI	CCTNNNNNAGG	1 cut(s)	360
BstF5I	GGATG	8 cut(s)	76, 139, 162, 1489, 1676, 1696, 1709, 1859
BstH2I	RGCGCY	1 cut(s)	384
BstHHI	GCGC	2 cut(s)	383, 1816
BstMAI	GTCTC	2 cut(s)	1319, 1755
BstNI	CCWGG	5 cut(s)	480, 875, 1557, 1701, 1867
BstNSI	RCATGY	2 cut(s)	772, 1669
BstSCI	CCNGG	7 cut(s)	478, 873, 890, 1555, 1699, 1713, 1865
BstSFI	CTRYAG	1 cut(s)	1193
BstSLI	GKGCMC	2 cut(s)	280, 1202
BstV1I	GCAGC	5 cut(s)	86, 430, 589, 1147, 1603
BstX2I	RGATCY	3 cut(s)	6, 789, 1504
BstXI	CCANNNNNNTGG	1 cut(s)	636
BstYI	RGATCY	3 cut(s)	6, 789, 1504
BsuRI	GGCC	5 cut(s)	438, 509, 640, 664, 890
BtsCI	GGATG	8 cut(s)	76, 139, 162, 1489, 1676, 1696, 1709, 1859
BtsI	GCAGTG	2 cut(s)	580, 1202
BtsIMutI	CAGTG	9 cut(s)	378, 505, 580, 961, 1053, 1202, 1389, 1440, 1851
BveI	ACCTGC	1 cut(s)	1190
Cac8I	GCNNGC	4 cut(s)	797, 861, 1140, 1596
CciI	TCATGA	2 cut(s)	715, 1207
CfoI	GCGC	2 cut(s)	383, 1816
Cfr13I	GGNCC	5 cut(s)	437, 692, 889, 1076, 1837
CseI	GACGC	2 cut(s)	224, 1662
CviAII	CATG	9 cut(s)	86, 402, 716, 769, 1208, 1660, 1666, 1684, 1875
DdeI	CTNAG	6 cut(s)	60, 201, 395, 453, 1080, 1479
DraI	TTTAAA	1 cut(s)	870
DraIII	CACNNNGTG	1 cut(s)	1642
Eam1104I	CTCTTC	3 cut(s)	146, 176, 1140
EarI	CTCTTC	3 cut(s)	146, 176, 1140
EciI	GGCGGA	1 cut(s)	479
Ecl136II	GAGCTC	2 cut(s)	145, 535
Eco130I	CCWWGG	4 cut(s)	632, 681, 840, 1219
Eco24I	GRGCYC	2 cut(s)	147, 537
Eco32I	GATATC	1 cut(s)	1500
Eco47I	GGWCC	3 cut(s)	692, 1076, 1837
Eco53kI	GAGCTC	2 cut(s)	145, 535
Eco57I	CTGAAG	4 cut(s)	481, 743, 1164, 1446
Eco88I	CYCGRG	1 cut(s)	146
EcoICRI	GAGCTC	2 cut(s)	145, 535
EcoNI	CCTNNNNNAGG	1 cut(s)	360
EcoO109I	RGGNCCY	2 cut(s)	692, 1076
EcoRI	GAATTC	1 cut(s)	1422
EcoRII	CCWGG	5 cut(s)	478, 873, 1555, 1699, 1865
EcoRV	GATATC	1 cut(s)	1500
EcoT14I	CCWWGG	4 cut(s)	632, 681, 840, 1219
EcoT22I	ATGCAT	1 cut(s)	1861
EcoT38I	GRGCYC	2 cut(s)	147, 537
ErhI	CCWWGG	4 cut(s)	632, 681, 840, 1219
FaeI	CATG	9 cut(s)	89, 405, 719, 772, 1211, 1663, 1669, 1687, 1878
FaqI	GGGAC	3 cut(s)	757, 1089, 1428
FatI	CATG	9 cut(s)	85, 401, 715, 768, 1207, 1659, 1665, 1683, 1874
FauI	CCCGC	1 cut(s)	1848
FbaI	TGATCA	1 cut(s)	1729
FblI	GTMKAC	1 cut(s)	1782
Fnu4HI	GCNGC	5 cut(s)	75, 444, 578, 1136, 1592
FokI	GGATG	8 cut(s)	83, 146, 169, 1496, 1663, 1683, 1696, 1846
FriOI	GRGCYC	2 cut(s)	147, 537
Fsp4HI	GCNGC	5 cut(s)	75, 444, 578, 1136, 1592
FspBI	CTAG	4 cut(s)	860, 947, 963, 1220
GlaI	GCGC	2 cut(s)	382, 1815
GluI	GCNGC	5 cut(s)	75, 444, 578, 1136, 1592
GsaI	CCCAGC	2 cut(s)	443, 1076
GsuI	CTGGAG	1 cut(s)	63
HaeII	RGCGCY	1 cut(s)	384
HaeIII	GGCC	5 cut(s)	438, 509, 640, 664, 890
HapII	CCGG	3 cut(s)	892, 1273, 1715
HgaI	GACGC	2 cut(s)	224, 1662
HhaI	GCGC	2 cut(s)	383, 1816
Hin1II	CATG	9 cut(s)	89, 405, 719, 772, 1211, 1663, 1669, 1687, 1878
Hin6I	GCGC	2 cut(s)	381, 1814
HinP1I	GCGC	2 cut(s)	381, 1814
HincII	GTYRAC	2 cut(s)	220, 1783
HindII	GTYRAC	2 cut(s)	220, 1783
HindIII	AAGCTT	4 cut(s)	797, 882, 1306, 1336
HinfI	GANTC	5 cut(s)	288, 941, 1409, 1748, 1796
HpaII	CCGG	3 cut(s)	892, 1273, 1715
HpyAV	CCTTC	8 cut(s)	59, 206, 403, 610, 721, 730, 974, 1165
HpyCH4IV	ACGT	3 cut(s)	601, 1447, 1818
HpyF3I	CTNAG	6 cut(s)	60, 201, 395, 453, 1080, 1479
HpySE526I	ACGT	3 cut(s)	601, 1447, 1818
Hsp92II	CATG	9 cut(s)	89, 405, 719, 772, 1211, 1663, 1669, 1687, 1878
HspAI	GCGC	2 cut(s)	381, 1814
Ksp22I	TGATCA	1 cut(s)	1729
LguI	GCTCTTC	1 cut(s)	1140
LmnI	GCTCC	5 cut(s)	58, 82, 532, 540, 1049
Lsp1109I	GCAGC	5 cut(s)	86, 430, 589, 1147, 1603
LweI	GCATC	5 cut(s)	61, 564, 1447, 1846, 1868
MaeI	CTAG	4 cut(s)	860, 947, 963, 1220
MaeII	ACGT	3 cut(s)	601, 1447, 1818
MaeIII	GTNAC	3 cut(s)	270, 1443, 1634
MflI	RGATCY	3 cut(s)	6, 789, 1504
MhlI	GDGCHC	4 cut(s)	147, 280, 537, 1202
MluCI	AATT	6 cut(s)	348, 1261, 1279, 1422, 1607, 1765
MlyI	GAGTC	3 cut(s)	935, 1757, 1790
MmeI	TCCRAC	2 cut(s)	591, 1650
Mph1103I	ATGCAT	1 cut(s)	1861
MroXI	GAANNNNTTC	1 cut(s)	1765
MseI	TTAA	4 cut(s)	869, 1032, 1086, 1163
MslI	CAYNNNNRTG	5 cut(s)	498, 634, 755, 1664, 1688
MspA1I	CMGCKG	2 cut(s)	443, 1138
MspI	CCGG	3 cut(s)	892, 1273, 1715
MspR9I	CCNGG	7 cut(s)	480, 875, 892, 1557, 1701, 1715, 1867
Mva1269I	GAATGC	1 cut(s)	177
MvaI	CCWGG	5 cut(s)	480, 875, 1557, 1701, 1867
NciI	CCSGG	2 cut(s)	892, 1715
NheI	GCTAGC	1 cut(s)	859
NlaIII	CATG	9 cut(s)	89, 405, 719, 772, 1211, 1663, 1669, 1687, 1878
NlaIV	GGNNCC	6 cut(s)	693, 1051, 1077, 1674, 1839, 1871
NmuCI	GTSAC	3 cut(s)	270, 1443, 1634
NsiI	ATGCAT	1 cut(s)	1861
NspI	RCATGY	2 cut(s)	772, 1669
OliI	CACNNNNGTG	3 cut(s)	498, 634, 755
PaeR7I	CTCGAG	1 cut(s)	146
PagI	TCATGA	2 cut(s)	715, 1207
PaqCI	CACCTGC	1 cut(s)	1190
PasI	CCCWGGG	1 cut(s)	479
PciI	ACATGT	2 cut(s)	768, 1665
PciSI	GCTCTTC	1 cut(s)	1140
PctI	GAATGC	1 cut(s)	177
PdmI	GAANNNNTTC	1 cut(s)	1765
PfeI	GAWTC	2 cut(s)	288, 1409
PflFI	GACNNNGTC	1 cut(s)	234
PflMI	CCANNNNNTGG	2 cut(s)	377, 929
PfoI	TCCNGGA	1 cut(s)	1555
PkrI	GCNGC	5 cut(s)	76, 445, 579, 1137, 1593
PleI	GAGTC	3 cut(s)	935, 1756, 1790
PpsI	GAGTC	3 cut(s)	935, 1756, 1790
Ppu21I	YACGTR	1 cut(s)	1448
PpuMI	RGGWCCY	2 cut(s)	692, 1076
PscI	ACATGT	2 cut(s)	768, 1665
PsiI	TTATAA	1 cut(s)	1827
Psp124BI	GAGCTC	2 cut(s)	147, 537
Psp5II	RGGWCCY	2 cut(s)	692, 1076
Psp6I	CCWGG	5 cut(s)	478, 873, 1555, 1699, 1865
PspFI	CCCAGC	2 cut(s)	439, 1072
PspGI	CCWGG	5 cut(s)	478, 873, 1555, 1699, 1865
PspN4I	GGNNCC	6 cut(s)	693, 1051, 1077, 1674, 1839, 1871
PspPI	GGNCC	5 cut(s)	437, 692, 889, 1076, 1837
PspPPI	RGGWCCY	2 cut(s)	692, 1076
PstI	CTGCAG	1 cut(s)	1197
PsuI	RGATCY	3 cut(s)	6, 789, 1504
PsyI	GACNNNGTC	1 cut(s)	234
PvuII	CAGCTG	2 cut(s)	443, 1138
RseI	CAYNNNNRTG	5 cut(s)	498, 634, 755, 1664, 1688
SacI	GAGCTC	2 cut(s)	147, 537
SalI	GTCGAC	1 cut(s)	1781
SapI	GCTCTTC	1 cut(s)	1140
SaqAI	TTAA	4 cut(s)	869, 1032, 1086, 1163
SatI	GCNGC	5 cut(s)	75, 444, 578, 1136, 1592
Sau96I	GGNCC	5 cut(s)	437, 692, 889, 1076, 1837
SchI	GAGTC	3 cut(s)	935, 1757, 1790
ScrFI	CCNGG	7 cut(s)	480, 875, 892, 1557, 1701, 1715, 1867
SduI	GDGCHC	4 cut(s)	147, 280, 537, 1202
SfaNI	GCATC	5 cut(s)	61, 564, 1447, 1846, 1868
SfcI	CTRYAG	1 cut(s)	1193
Sfr274I	CTCGAG	1 cut(s)	146
SinI	GGWCC	3 cut(s)	692, 1076, 1837
SlaI	CTCGAG	1 cut(s)	146
SmiMI	CAYNNNNRTG	5 cut(s)	498, 634, 755, 1664, 1688
SmlI	CTYRAG	2 cut(s)	146, 330
SmoI	CTYRAG	2 cut(s)	146, 330
SpeI	ACTAGT	1 cut(s)	962
Sse9I	AATT	6 cut(s)	348, 1261, 1279, 1422, 1607, 1765
SsiI	CCGC	4 cut(s)	464, 503, 793, 1841
SspI	AATATT	1 cut(s)	1531
SspMI	CTAG	4 cut(s)	860, 947, 963, 1220
SstI	GAGCTC	2 cut(s)	147, 537
StyD4I	CCNGG	7 cut(s)	478, 873, 890, 1555, 1699, 1713, 1865
StyI	CCWWGG	4 cut(s)	632, 681, 840, 1219
TaiI	ACGT	3 cut(s)	604, 1450, 1821
TaqI	TCGA	3 cut(s)	147, 978, 1782
TasI	AATT	6 cut(s)	348, 1261, 1279, 1422, 1607, 1765
TfiI	GAWTC	2 cut(s)	288, 1409
Tru1I	TTAA	4 cut(s)	869, 1032, 1086, 1163
Tru9I	TTAA	4 cut(s)	869, 1032, 1086, 1163
TscAI	CASTG	9 cut(s)	378, 505, 587, 961, 1060, 1202, 1389, 1440, 1851
TseFI	GTSAC	3 cut(s)	270, 1443, 1634
TseI	GCWGC	5 cut(s)	74, 443, 577, 1135, 1591
Tsp45I	GTSAC	3 cut(s)	270, 1443, 1634
TspDTI	ATGAA	7 cut(s)	102, 138, 504, 1631, 1676, 1685, 1698
TspRI	CASTG	9 cut(s)	378, 505, 587, 961, 1060, 1202, 1389, 1440, 1851
Tth111I	GACNNNGTC	1 cut(s)	234
Van91I	CCANNNNNTGG	2 cut(s)	377, 929
VpaK11BI	GGWCC	3 cut(s)	692, 1076, 1837
XagI	CCTNNNNNAGG	1 cut(s)	360
XapI	RAATTY	1 cut(s)	1422
XceI	RCATGY	2 cut(s)	772, 1669
XhoI	CTCGAG	1 cut(s)	146
XmaJI	CCTAGG	1 cut(s)	1219
XmiI	GTMKAC	1 cut(s)	1782
XmnI	GAANNNNTTC	1 cut(s)	1765
XspI	CTAG	4 cut(s)	860, 947, 963, 1220
Zsp2I	ATGCAT	1 cut(s)	1861

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.