Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0001963

Physical Location & Seq

Genomic Coordinates Reverse (-)

32047599 .. 32049431

Gene Structure 1833 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1266300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1833 bp

ATGGCGATCATTGAGGAGTTGGAGTTCAGTGACGACCTTGACTTTCTTTTGGCTTTCCCTGAGGAAGGTGAGGCAGCCCCAGAGAATGACCCAGAGGCAACAGTGGAGGAGGATGATAGTGACGACGAATTGGATGTTAATGAGCTTGAGAGGAGGGTGTGGAAATATAGAATGCGACTGAAGCGGCTTAAAGAGCAAAATGAGGGAACAGAAGCAGCTGACAATGCTAGGCAGTGTCAGCCACAGGAACAAGCTCGGAGGAAGAGGATGTCACGGGCACAAGATGGAATCCTGAAATATATGTTAAAAATGATGGAAGTTTGTAAAGCCAAGGGTTTTGTTTATGGGATTATCCCCGAAAATGGTAAACCAGTGACTGGTGCGTCTGACAGTCTTCGCGAATGGTGGAAGGAAAAAGTAAGATTTGACCATAATGGCCCTGCTGCAATTTCTAAGTATCTGGCTGAACATCCAATCCCTGGGAAGAATGAAGACTCCAATGCAGTACCATCCACTCCTCATAACTTACAGGAGCTCCATGATGCCACTCTTGGTTCACTTTTGTCGGCTTTGATGCAGCACTGCCATCCACCCCAAAGACGTTTCCCGTTGGAGAAGGGTGTTGCCCCACCTTGGTGGCCCACTGGCAATGAAGAATGGTGGACTCAATTGAATCTGCGCAAGGATCAGGGTCCTCCCCCATACAGGAAGCCTCATGATCTGAAGAAGGCTTGGAAGGTGAGTGTTCTCGCGGCTGTGATAAAGCACATGTCACCCGATATTGCCAGGATCCGGAAGCTTGTTCATCGGTCTAAATATTTGCAGGACAAAATGTCTGCTAAGGAGACCACCATTTGGCTAGCCATCCTTAACCAGGAGGAAAATTTGGCCCAGAGGTTATATCCTGATGGATGTACACCTCCATCATCTTCTGGGAGTGGGTCTAATTTTGCAATCAGCGGTACCAGTGATTATGATGTTGAAGTGGTTGATGATGAAGATAATTATGAAGAAGAGGACTGCCAACCTCTTTTTAATCACTTAAATATTGGAACATCTGGACACAGAGATGGACTGATGCATCATTTTATGGCAGAGCTGACTGAGACCGATTCAGACTCTGGTCAAAAGAGGAAGCAGCTGCCTGAAGAGCCACAAATGATGCTAAATCATCAGGGTTACACCTGCGAATATCTGGATTGCCCGTATCATGATTACCACCTAGGCTTTCGCAACATAACTGCAAGGAATAATCACCAGTTGACGTGTTCTTACAGGAATAATTCTTCACAAGTTGGAATGTCAAGCGTTCATCCTAACAGTGACAAACTTGTGGGGTCCTTATCCAGTCCTCAACCTAATCCAGCTATTCAACAACCAGTGAACCAGACAAGTAGTTTTAATGCTTCCGGACTTGAACTTGCTGGGGATGAGCAGAATATGATATCTGAGTTTATGTCATTCTGTGATGGTAATATTCAGCAGAACAAGAACTCGGATCCTGGAAGCCTCGATGTTGTAGAAAACCACAACCAGCATCAGGTGAATTATCAATTTCCCATGGGTGATAACTTCTTTGATGAAAGGTTGGTTATAGGATCCAACATGTCTCAAGCATCGCCTATGCCTATGCCTATGCTTCCTCCAGCTACTCCAGCTCCTCCATCTCTCCCATCCACAGAATTTCAGTTTGATCCGTGCACCTTGTTTGATTCTCCATTTGGTAATCATCCCAATAATACTGACAACTTAGGATTTGGCACCAACTTGCCGCGAGATGACTATAGTCTTGACTCGATGCTGGAGCAAGATCCATTCTGGATGTGTCGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000160 GO:0000976 GO:0001067 GO:0001666 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0006950 GO:0006952 GO:0007154 GO:0007165 GO:0008150 GO:0009605 GO:0009607 GO:0009617 GO:0009628 GO:0009719 GO:0009723 GO:0009725 GO:0009743 GO:0009755 GO:0009756 GO:0009873 GO:0009889 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010039 GO:0010182 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0023052 GO:0031323 GO:0031326 GO:0032870 GO:0035556 GO:0036293 GO:0042221 GO:0042742 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044212 GO:0044424 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051252 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0065007 GO:0070482 GO:0070887 GO:0071241 GO:0071248 GO:0071281 GO:0071310 GO:0071322 GO:0071369 GO:0071495 GO:0080090 GO:0097159 GO:0098542 GO:0140110 GO:1901363 GO:1901700 GO:1901701 GO:1903506 GO:1990837 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

ko04016 ko04075 map04016 map04075

Pfam Domains

Protein Families

EIN3

Protein Analysis

611

Amino Acids

69.09

Weight (kDa)

4.94

Isoelectric Point (pI)

56.82

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
EIN3_DNA-bd	PF04873	48 - 294	1.2e-122	Ethylene insensitive 3, DNA-binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0001963)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G27050 AT3G20770
fragaria_vesca	FvH4_1g28720 FvH4_1g28720 FvH4_7g04940 FvH4_7g04940 FvH4_7g04940 FvH4_7g04940 FvH4_7g04940
malus_domestica	MD02G1266200.v1.1 MD02G1266300.v1.1 MD07G1053500.v1.1 MD07G1053800.v1.1 MD08G1245800.v1.1 MD15G1441000.v1.1
prunus_persica	Prupe.2G058400_v2.0.a1 Prupe.2G058500_v2.0.a1 Prupe.2G070300_v2.0.a1
pyrus_communis	pycom02g22750 pycom02g22760 pycom07g04140 pycom07g04160 pycom08g21330 pycom15g38880
rosa_chinensis	RchiOBHm_Chr1g0331011 RchiOBHm_Chr5g0049961
rosa_laevigata	RLG00000029759 RLG00000034672
rosa_multiflora	Rmu_sc0000333.1_g000038 Rmu_sc0013622.1_g000002 Rmu_sc0013622.1_g000003
rosa_roxburghii	Rroxscaffold_1G00031290 Rroxscaffold_4G00319550
rosa_rugosa	Rorug01G0091600 Rorug05G0255200
rosa_samantha	Rh1AG113300 Rh1BG087200 Rh1CG110200 Rh1DG118900 Rh5AG329100 Rh5BG339600 Rh5CG365600 Rh5DG352200
rosa_wichuraiana	Rw1G009240 Rw5G031070

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1193
AasI	GACNNNNNNGTC	1 cut(s)	382
Acc16I	TGCGCA	1 cut(s)	680
Acc36I	ACCTGC	1 cut(s)	1193
Acc65I	GGTACC	1 cut(s)	962
AccB1I	GGYRCC	2 cut(s)	962, 1760
AccB7I	CCANNNNNTGG	3 cut(s)	377, 479, 855
AccII	CGCG	3 cut(s)	399, 752, 1774
AccIII	TCCGGA	2 cut(s)	792, 1409
AciI	CCGC	4 cut(s)	184, 752, 960, 1772
AclWI	GGATC	9 cut(s)	693, 784, 797, 1493, 1506, 1593, 1606, 1688, 1805
AcsI	RAATTY	2 cut(s)	883, 1682
AcuI	CTGAAG	3 cut(s)	200, 743, 1167
AfaI	GTAC	3 cut(s)	507, 916, 964
AflIII	ACRYGT	3 cut(s)	768, 1265, 1605
AgsI	TTSAA	4 cut(s)	673, 983, 1373, 1418
AhdI	GACNNNNNGTC	1 cut(s)	832
AjiI	CACGTC	1 cut(s)	1266
AjnI	CCWGG	4 cut(s)	478, 785, 873, 1501
AleI	CACNNNNGTG	1 cut(s)	634
AloI	GAACNNNNNNTCC	4 cut(s)	8, 40, 459, 491
Alw21I	GWGCWC	2 cut(s)	537, 1703
Alw26I	GTCTC	3 cut(s)	839, 1100, 1614
Alw44I	GTGCAC	1 cut(s)	1699
AlwI	GGATC	9 cut(s)	693, 784, 797, 1493, 1506, 1593, 1606, 1688, 1805
AlwNI	CAGNNNCTG	2 cut(s)	377, 1121
Aor13HI	TCCGGA	2 cut(s)	792, 1409
AoxI	GGCC	3 cut(s)	436, 638, 888
ApaLI	GTGCAC	1 cut(s)	1699
ApeKI	GCWGC	6 cut(s)	74, 215, 443, 577, 1138, 1141
ApoI	RAATTY	2 cut(s)	883, 1682
Asp718I	GGTACC	1 cut(s)	962
AspA2I	CCTAGG	1 cut(s)	1222
AspLEI	GCGC	1 cut(s)	681
AspS9I	GGNCC	5 cut(s)	437, 639, 692, 889, 1338
AsuHPI	GGTGA	6 cut(s)	80, 751, 765, 1247, 1555, 1577
AsuNHI	GCTAGC	1 cut(s)	859
AvaII	GGWCC	2 cut(s)	692, 1338
AvrII	CCTAGG	1 cut(s)	1222
AxyI	CCTNAGG	1 cut(s)	60
BaeGI	GKGCMC	2 cut(s)	280, 1703
BamHI	GGATCC	3 cut(s)	789, 1498, 1598
BanI	GGYRCC	2 cut(s)	962, 1760
BanII	GRGCYC	1 cut(s)	537
BbsI	GAAGAC	2 cut(s)	386, 498
Bbv12I	GWGCWC	2 cut(s)	537, 1703
BbvI	GCAGC	6 cut(s)	86, 227, 430, 589, 1128, 1150
BciT130I	CCWGG	4 cut(s)	480, 787, 875, 1503
BcoDI	GTCTC	3 cut(s)	839, 1100, 1614
BfaI	CTAG	3 cut(s)	228, 860, 1223
BfmI	CTRYAG	1 cut(s)	1783
BfuAI	ACCTGC	1 cut(s)	1193
BisI	GCNGC	9 cut(s)	75, 185, 216, 444, 578, 753, 1139, 1142, 1772
BlnI	CCTAGG	1 cut(s)	1222
BlsI	GCNGC	9 cut(s)	76, 186, 217, 445, 579, 754, 1140, 1143, 1773
Bme1390I	CCNGG	4 cut(s)	480, 787, 875, 1503
Bme18I	GGWCC	2 cut(s)	692, 1338
BmeRI	GACNNNNNGTC	1 cut(s)	832
BmgBI	CACGTC	1 cut(s)	1266
BmgT120I	GGNCC	5 cut(s)	437, 639, 692, 889, 1338
BmiI	GGNNCC	7 cut(s)	693, 791, 964, 1339, 1500, 1600, 1762
BmrFI	CCNGG	4 cut(s)	480, 787, 875, 1503
BmsI	GCATC	8 cut(s)	532, 564, 1068, 1090, 1152, 1546, 1625, 1788
BmtI	GCTAGC	1 cut(s)	863
BoxI	GACNNNNGTC	2 cut(s)	1122, 1785
BpiI	GAAGAC	2 cut(s)	386, 498
BpmI	CTGGAG	3 cut(s)	1629, 1638, 1823
Bpu10I	CCTNAGC	1 cut(s)	840
BpuEI	CTTGAG	2 cut(s)	167, 1596
BsaI	GGTCTC	2 cut(s)	839, 1100
BsaJI	CCNNGG	6 cut(s)	330, 478, 479, 632, 1222, 1560
BsaWI	WCCGGW	2 cut(s)	792, 1409
BsaXI	ACNNNNNCTCC	6 cut(s)	8, 38, 519, 549, 1642, 1672
Bse1I	ACTGG	7 cut(s)	371, 382, 649, 966, 1258, 1347, 1379
Bse21I	CCTNAGG	1 cut(s)	60
Bse3DI	GCAATG	1 cut(s)	655
BseAI	TCCGGA	2 cut(s)	792, 1409
BseBI	CCWGG	4 cut(s)	480, 787, 875, 1503
BseDI	CCNNGG	6 cut(s)	330, 478, 479, 632, 1222, 1560
BseMI	GCAATG	1 cut(s)	655
BseMII	CTCAG	3 cut(s)	51, 1095, 1440
BseNI	ACTGG	7 cut(s)	371, 382, 649, 966, 1258, 1347, 1379
BseRI	GAGGAG	5 cut(s)	29, 122, 166, 507, 1650
BseSI	GKGCMC	2 cut(s)	280, 1703
BseXI	GCAGC	6 cut(s)	86, 227, 430, 589, 1128, 1150
BseYI	CCCAGC	1 cut(s)	1424
Bsh1236I	CGCG	3 cut(s)	399, 752, 1774
BshFI	GGCC	3 cut(s)	438, 640, 890
BshNI	GGYRCC	2 cut(s)	962, 1760
BsiHKAI	GWGCWC	2 cut(s)	537, 1703
BsiSI	CCGG	2 cut(s)	793, 1410
BsmAI	GTCTC	3 cut(s)	839, 1100, 1614
BsmI	GAATGC	1 cut(s)	177
BsnI	GGCC	3 cut(s)	438, 640, 890
Bso31I	GGTCTC	2 cut(s)	839, 1100
Bsp1286I	GDGCHC	3 cut(s)	280, 537, 1703
Bsp13I	TCCGGA	2 cut(s)	792, 1409
Bsp1407I	TGTACA	1 cut(s)	914
Bsp143I	GATC	8 cut(s)	6, 685, 718, 789, 1498, 1598, 1693, 1810
Bsp19I	CCATGG	1 cut(s)	1560
Bsp68I	TCGCGA	1 cut(s)	399
BspACI	CCGC	4 cut(s)	184, 752, 960, 1772
BspANI	GGCC	3 cut(s)	438, 640, 890
BspCNI	CTCAG	3 cut(s)	52, 1096, 1441
BspEI	TCCGGA	2 cut(s)	792, 1409
BspFNI	CGCG	3 cut(s)	399, 752, 1774
BspHI	TCATGA	2 cut(s)	715, 1210
BspLI	GGNNCC	7 cut(s)	693, 791, 964, 1339, 1500, 1600, 1762
BspMI	ACCTGC	1 cut(s)	1193
BspOI	GCTAGC	1 cut(s)	863
BspPI	GGATC	9 cut(s)	693, 784, 797, 1493, 1506, 1593, 1606, 1688, 1805
BspQI	GCTCTTC	1 cut(s)	1143
BspT107I	GGYRCC	2 cut(s)	962, 1760
BspTNI	GGTCTC	2 cut(s)	839, 1100
BsrDI	GCAATG	1 cut(s)	655
BsrGI	TGTACA	1 cut(s)	914
BsrI	ACTGG	7 cut(s)	371, 382, 649, 966, 1258, 1347, 1379
BssECI	CCNNGG	6 cut(s)	330, 478, 479, 632, 1222, 1560
BssMI	GATC	8 cut(s)	6, 685, 718, 789, 1498, 1598, 1693, 1810
BssT1I	CCWWGG	4 cut(s)	330, 632, 1222, 1560
Bst2UI	CCWGG	4 cut(s)	480, 787, 875, 1503
Bst4CI	ACNGT	3 cut(s)	103, 392, 1322
Bst6I	CTCTTC	3 cut(s)	257, 1008, 1143
BstAUI	TGTACA	1 cut(s)	914
BstC8I	GCNNGC	1 cut(s)	861
BstDEI	CTNAG	6 cut(s)	60, 453, 840, 1104, 1449, 1750
BstDSI	CCRYGG	1 cut(s)	1560
BstENI	CCTNNNNNAGG	2 cut(s)	63, 872
BstFNI	CGCG	3 cut(s)	399, 752, 1774
BstHHI	GCGC	1 cut(s)	681
BstKTI	GATC	8 cut(s)	9, 688, 721, 792, 1501, 1601, 1696, 1813
BstMAI	GTCTC	3 cut(s)	839, 1100, 1614
BstMBI	GATC	8 cut(s)	6, 685, 718, 789, 1498, 1598, 1693, 1810
BstMWI	GCNNNNNNNGC	6 cut(s)	181, 193, 224, 238, 1150, 1655
BstNI	CCWGG	4 cut(s)	480, 787, 875, 1503
BstNSI	RCATGY	2 cut(s)	772, 1609
BstPAI	GACNNNNGTC	2 cut(s)	1122, 1785
BstSCI	CCNGG	4 cut(s)	478, 785, 873, 1501
BstSFI	CTRYAG	1 cut(s)	1783
BstSLI	GKGCMC	2 cut(s)	280, 1703
BstUI	CGCG	3 cut(s)	399, 752, 1774
BstV1I	GCAGC	6 cut(s)	86, 227, 430, 589, 1128, 1150
BstV2I	GAAGAC	2 cut(s)	386, 498
BstX2I	RGATCY	4 cut(s)	789, 1498, 1598, 1810
BstXI	CCANNNNNNTGG	1 cut(s)	636
BstYI	RGATCY	4 cut(s)	789, 1498, 1598, 1810
Bsu36I	CCTNAGG	1 cut(s)	60
BsuRI	GGCC	3 cut(s)	438, 640, 890
BtgI	CCRYGG	1 cut(s)	1560
BtgZI	GCGATG	1 cut(s)	1602
BtrI	CACGTC	1 cut(s)	1266
BtsI	GCAGTG	2 cut(s)	239, 580
BtsIMutI	CAGTG	9 cut(s)	34, 108, 239, 378, 580, 642, 973, 1327, 1386
BtuMI	TCGCGA	1 cut(s)	399
BveI	ACCTGC	1 cut(s)	1193
Cac8I	GCNNGC	1 cut(s)	861
CaiI	CAGNNNCTG	2 cut(s)	377, 1121
CciI	TCATGA	2 cut(s)	715, 1210
CfoI	GCGC	1 cut(s)	681
Cfr13I	GGNCC	5 cut(s)	437, 639, 692, 889, 1338
CseI	GACGC	1 cut(s)	372
Csp6I	GTAC	3 cut(s)	506, 915, 963
CviAII	CATG	6 cut(s)	539, 716, 769, 1211, 1561, 1606
CviQI	GTAC	3 cut(s)	506, 915, 963
DdeI	CTNAG	6 cut(s)	60, 453, 840, 1104, 1449, 1750
DpnI	GATC	8 cut(s)	8, 687, 720, 791, 1500, 1600, 1695, 1812
DpnII	GATC	8 cut(s)	6, 685, 718, 789, 1498, 1598, 1693, 1810
DrdI	GACNNNNNNGTC	1 cut(s)	382
DriI	GACNNNNNGTC	1 cut(s)	832
DseDI	GACNNNNNNGTC	1 cut(s)	382
Eam1104I	CTCTTC	3 cut(s)	257, 1008, 1143
Eam1105I	GACNNNNNGTC	1 cut(s)	832
EarI	CTCTTC	3 cut(s)	257, 1008, 1143
Ecl136II	GAGCTC	1 cut(s)	535
Eco130I	CCWWGG	4 cut(s)	330, 632, 1222, 1560
Eco24I	GRGCYC	1 cut(s)	537
Eco31I	GGTCTC	2 cut(s)	839, 1100
Eco32I	GATATC	1 cut(s)	1446
Eco47I	GGWCC	2 cut(s)	692, 1338
Eco53kI	GAGCTC	1 cut(s)	535
Eco57I	CTGAAG	3 cut(s)	200, 743, 1167
Eco81I	CCTNAGG	1 cut(s)	60
EcoICRI	GAGCTC	1 cut(s)	535
EcoNI	CCTNNNNNAGG	2 cut(s)	63, 872
EcoO109I	RGGNCCY	2 cut(s)	692, 1338
EcoRII	CCWGG	4 cut(s)	478, 785, 873, 1501
EcoRV	GATATC	1 cut(s)	1446
EcoT14I	CCWWGG	4 cut(s)	330, 632, 1222, 1560
EcoT22I	ATGCAT	1 cut(s)	1083
EcoT38I	GRGCYC	1 cut(s)	537
ErhI	CCWWGG	4 cut(s)	330, 632, 1222, 1560
FaeI	CATG	6 cut(s)	542, 719, 772, 1214, 1564, 1609
FatI	CATG	6 cut(s)	538, 715, 768, 1210, 1560, 1605
Fnu4HI	GCNGC	9 cut(s)	75, 185, 216, 444, 578, 753, 1139, 1142, 1772
FriOI	GRGCYC	1 cut(s)	537
Fsp4HI	GCNGC	9 cut(s)	75, 185, 216, 444, 578, 753, 1139, 1142, 1772
FspBI	CTAG	3 cut(s)	228, 860, 1223
FspI	TGCGCA	1 cut(s)	680
GlaI	GCGC	1 cut(s)	680
GluI	GCNGC	9 cut(s)	75, 185, 216, 444, 578, 753, 1139, 1142, 1772
GsaI	CCCAGC	1 cut(s)	1428
GsuI	CTGGAG	3 cut(s)	1629, 1638, 1823
HaeIII	GGCC	3 cut(s)	438, 640, 890
HapII	CCGG	2 cut(s)	793, 1410
HgaI	GACGC	1 cut(s)	372
HhaI	GCGC	1 cut(s)	681
Hin1II	CATG	6 cut(s)	542, 719, 772, 1214, 1564, 1609
Hin6I	GCGC	1 cut(s)	679
HinP1I	GCGC	1 cut(s)	679
HincII	GTYRAC	1 cut(s)	1263
HindII	GTYRAC	1 cut(s)	1263
HindIII	AAGCTT	1 cut(s)	797
HinfI	GANTC	8 cut(s)	288, 494, 664, 673, 1112, 1118, 1712, 1793
HpaII	CCGG	2 cut(s)	793, 1410
HphI	GGTGA	6 cut(s)	80, 751, 765, 1247, 1555, 1577
Hpy166II	GTNNAC	7 cut(s)	368, 557, 663, 917, 1263, 1384, 1701
Hpy188I	TCNGA	6 cut(s)	258, 388, 723, 1117, 1450, 1498
Hpy8I	GTNNAC	7 cut(s)	368, 557, 663, 917, 1263, 1384, 1701
Hpy99I	CGWCG	1 cut(s)	128
HpyAV	CCTTC	5 cut(s)	59, 403, 610, 721, 730
HpyCH4III	ACNGT	3 cut(s)	103, 392, 1322
HpyCH4IV	ACGT	2 cut(s)	601, 1265
HpyCH4V	TGCA	8 cut(s)	446, 503, 577, 823, 953, 1081, 1244, 1701
HpyF10VI	GCNNNNNNNGC	6 cut(s)	181, 193, 224, 238, 1150, 1655
HpyF3I	CTNAG	6 cut(s)	60, 453, 840, 1104, 1449, 1750
HpySE526I	ACGT	2 cut(s)	601, 1265
Hsp92II	CATG	6 cut(s)	542, 719, 772, 1214, 1564, 1609
HspAI	GCGC	1 cut(s)	679
Kpn2I	TCCGGA	2 cut(s)	792, 1409
KpnI	GGTACC	1 cut(s)	966
Kzo9I	GATC	8 cut(s)	6, 685, 718, 789, 1498, 1598, 1693, 1810
LguI	GCTCTTC	1 cut(s)	1143
LmnI	GCTCC	4 cut(s)	532, 540, 1663, 1804
Lsp1109I	GCAGC	6 cut(s)	86, 227, 430, 589, 1128, 1150
LweI	GCATC	8 cut(s)	532, 564, 1068, 1090, 1152, 1546, 1625, 1788
MaeI	CTAG	3 cut(s)	228, 860, 1223
MaeII	ACGT	2 cut(s)	601, 1265
MaeIII	GTNAC	7 cut(s)	29, 119, 270, 373, 771, 1178, 1322
MalI	GATC	8 cut(s)	8, 687, 720, 791, 1500, 1600, 1695, 1812
MboI	GATC	8 cut(s)	6, 685, 718, 789, 1498, 1598, 1693, 1810
MfeI	CAATTG	1 cut(s)	668
MflI	RGATCY	4 cut(s)	789, 1498, 1598, 1810
MhlI	GDGCHC	3 cut(s)	280, 537, 1703
MlyI	GAGTC	4 cut(s)	488, 658, 1112, 1787
MmeI	TCCRAC	3 cut(s)	591, 1276, 1626
Mph1103I	ATGCAT	1 cut(s)	1083
MroI	TCCGGA	2 cut(s)	792, 1409
MseI	TTAA	7 cut(s)	138, 189, 305, 870, 1035, 1043, 1401
MslI	CAYNNNNRTG	2 cut(s)	634, 1068
MspA1I	CMGCKG	3 cut(s)	218, 960, 1141
MspI	CCGG	2 cut(s)	793, 1410
MspR9I	CCNGG	4 cut(s)	480, 787, 875, 1503
MunI	CAATTG	1 cut(s)	668
Mva1269I	GAATGC	1 cut(s)	177
MvaI	CCWGG	4 cut(s)	480, 787, 875, 1503
MvnI	CGCG	3 cut(s)	399, 752, 1774
MwoI	GCNNNNNNNGC	6 cut(s)	181, 193, 224, 238, 1150, 1655
NcoI	CCATGG	1 cut(s)	1560
NdeII	GATC	8 cut(s)	6, 685, 718, 789, 1498, 1598, 1693, 1810
NheI	GCTAGC	1 cut(s)	859
NlaIII	CATG	6 cut(s)	542, 719, 772, 1214, 1564, 1609
NlaIV	GGNNCC	7 cut(s)	693, 791, 964, 1339, 1500, 1600, 1762
NmuCI	GTSAC	6 cut(s)	29, 119, 270, 373, 771, 1322
NruI	TCGCGA	1 cut(s)	399
NsbI	TGCGCA	1 cut(s)	680
NsiI	ATGCAT	1 cut(s)	1083
NspI	RCATGY	2 cut(s)	772, 1609
OliI	CACNNNNGTG	1 cut(s)	634
PagI	TCATGA	2 cut(s)	715, 1210
PaqCI	CACCTGC	1 cut(s)	1193
PasI	CCCWGGG	1 cut(s)	479
PciI	ACATGT	2 cut(s)	768, 1605
PciSI	GCTCTTC	1 cut(s)	1143
PctI	GAATGC	1 cut(s)	177
PfeI	GAWTC	4 cut(s)	288, 673, 1112, 1712
PflMI	CCANNNNNTGG	3 cut(s)	377, 479, 855
PfoI	TCCNGGA	1 cut(s)	1501
PkrI	GCNGC	9 cut(s)	76, 186, 217, 445, 579, 754, 1140, 1143, 1773
PleI	GAGTC	4 cut(s)	488, 658, 1112, 1787
PpsI	GAGTC	4 cut(s)	488, 658, 1112, 1787
PpuMI	RGGWCCY	2 cut(s)	692, 1338
PscI	ACATGT	2 cut(s)	768, 1605
PshAI	GACNNNNGTC	2 cut(s)	1122, 1785
Psp124BI	GAGCTC	1 cut(s)	537
Psp5II	RGGWCCY	2 cut(s)	692, 1338
Psp6I	CCWGG	4 cut(s)	478, 785, 873, 1501
PspFI	CCCAGC	1 cut(s)	1424
PspGI	CCWGG	4 cut(s)	478, 785, 873, 1501
PspN4I	GGNNCC	7 cut(s)	693, 791, 964, 1339, 1500, 1600, 1762
PspPI	GGNCC	5 cut(s)	437, 639, 692, 889, 1338
PspPPI	RGGWCCY	2 cut(s)	692, 1338
PstNI	CAGNNNCTG	2 cut(s)	377, 1121
PsuI	RGATCY	4 cut(s)	789, 1498, 1598, 1810
PvuII	CAGCTG	2 cut(s)	218, 1141
RruI	TCGCGA	1 cut(s)	399
RsaI	GTAC	3 cut(s)	507, 916, 964
RsaNI	GTAC	3 cut(s)	506, 915, 963
RseI	CAYNNNNRTG	2 cut(s)	634, 1068
SacI	GAGCTC	1 cut(s)	537
SapI	GCTCTTC	1 cut(s)	1143
SaqAI	TTAA	7 cut(s)	138, 189, 305, 870, 1035, 1043, 1401
SatI	GCNGC	9 cut(s)	75, 185, 216, 444, 578, 753, 1139, 1142, 1772
Sau3AI	GATC	8 cut(s)	6, 685, 718, 789, 1498, 1598, 1693, 1810
Sau96I	GGNCC	5 cut(s)	437, 639, 692, 889, 1338
SchI	GAGTC	4 cut(s)	488, 658, 1112, 1787
ScrFI	CCNGG	4 cut(s)	480, 787, 875, 1503
SduI	GDGCHC	3 cut(s)	280, 537, 1703
SfaNI	GCATC	8 cut(s)	532, 564, 1068, 1090, 1152, 1546, 1625, 1788
SfcI	CTRYAG	1 cut(s)	1783
SinI	GGWCC	2 cut(s)	692, 1338
SmiMI	CAYNNNNRTG	2 cut(s)	634, 1068
SmlI	CTYRAG	2 cut(s)	146, 1611
SmoI	CTYRAG	2 cut(s)	146, 1611
SsiI	CCGC	4 cut(s)	184, 752, 960, 1772
SspI	AATATT	3 cut(s)	818, 1048, 1477
SspMI	CTAG	3 cut(s)	228, 860, 1223
SstI	GAGCTC	1 cut(s)	537
StyD4I	CCNGG	4 cut(s)	478, 785, 873, 1501
StyI	CCWWGG	4 cut(s)	330, 632, 1222, 1560
TaaI	ACNGT	3 cut(s)	103, 392, 1322
TaiI	ACGT	2 cut(s)	604, 1268
TaqI	TCGA	2 cut(s)	1512, 1796
TaqII	GACCGA	2 cut(s)	798, 1124
TatI	WGTACW	1 cut(s)	914
TauI	GCSGC	3 cut(s)	187, 755, 1774
TfiI	GAWTC	4 cut(s)	288, 673, 1112, 1712
Tru1I	TTAA	7 cut(s)	138, 189, 305, 870, 1035, 1043, 1401
Tru9I	TTAA	7 cut(s)	138, 189, 305, 870, 1035, 1043, 1401
TscAI	CASTG	9 cut(s)	34, 108, 239, 378, 587, 649, 973, 1327, 1386
TseFI	GTSAC	6 cut(s)	29, 119, 270, 373, 771, 1322
TseI	GCWGC	6 cut(s)	74, 215, 443, 577, 1138, 1141
Tsp45I	GTSAC	6 cut(s)	29, 119, 270, 373, 771, 1322
TspDTI	ATGAA	7 cut(s)	504, 666, 794, 1011, 1023, 1301, 1596
TspGWI	ACGGA	1 cut(s)	1686
TspRI	CASTG	9 cut(s)	34, 108, 239, 378, 587, 649, 973, 1327, 1386
Van91I	CCANNNNNTGG	3 cut(s)	377, 479, 855
VneI	GTGCAC	1 cut(s)	1699
VpaK11BI	GGWCC	2 cut(s)	692, 1338
XagI	CCTNNNNNAGG	2 cut(s)	63, 872
XapI	RAATTY	2 cut(s)	883, 1682
XceI	RCATGY	2 cut(s)	772, 1609
XmaJI	CCTAGG	1 cut(s)	1222
XspI	CTAG	3 cut(s)	228, 860, 1223
Zsp2I	ATGCAT	1 cut(s)	1083

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.