Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0001963

Physical Location & Seq

Genomic Coordinates Reverse (-)

54074550 .. 54078399

Gene Structure 3850 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1441000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1980 bp

ATGGTGATCTTTGAGGAGTTAGGTTTCTCTGGTAATCTTGACTATCTTTTGGCTCCATCTGGGGAAGGGGATGCAGCTCCAGAACATGAACAAGAGGCAACTGTGGAGGAGGATTATAGTGATGAAGAAATGGATGTTGATGAGCTTGAGAAGAGGATGTGGAGAGATCGAATGCTACTGAAGAGGCTGAAAGAACAAACTAAGGGAAAGGAAGGAGTTGACAATGCAAGACAGCGTCAATCACAGGAACAAGCGCGGAGGAAGAAGATGTCACGGGCACAAGATGGAATCCTGAAATATATGCTGAAAATGATGGAAGTTTGCAAAGCTCAGGGTTTTGTTTATGGAATTATCCCTGAAAAAGGAAAACCAGTGAGTGGCGCCTCTGACAATCTGCGAGCATGGTGGAAGGAAAAAGTAAGATTTGATCGTAATGGCCCGGCTGCGATTTCTAAGTATCAGGCAGATCATTCAATCCCTGGGAAGAATGAAGACTTCAGTGCAGTGGCATCCACTCCTCACACCTTACAGGAGCTCCAAGACACCACTCTTGGTTCACTTTTGTCAGCTTTGATGCAGCACTGCAATCCACCCCAAAGACGTTTCCCGTTGGAGAAGGGTGTTGCTCCTCCTTGGTGGCCTACTGGTAACGAGGAATGGTGGCCGCAACTAAATCTCCCCCAGGATCAGTGTCCTCCCCCATACAAGAAGCCTCATGATCTGAAGAAGGCTTGGAAGGTTGGTGTTCTCACGGCTGTGATTAAGCACATGTCGCCAGATATTGCAAAGATCCGCAAGCTTGTTCGTCAGTCCAAATGTTTGCAGGACAAAATGACTGCTAAGGAGAGCGCCACTTGGCTAGCCATTATAAACCAGGAGGAAGCTCTGGCCCGGAGGCTATATCCTGATAGATGTCCACCTCCATTTGCTGGTGGGAGTGAGTCTCTCGCAATCAGTGGTACTAGTGATTATGATGTTGAAGGGGTTGATGATGACGAAAATGTTGAAATAGAGGATTGCAAACCTCTTGTTAATCACTTCATCATTGGAGCGACTGGACAAAGAGAGAGGCAGGTGGTACCTCAGGTTAAGGGAGAGCACATCGAGATCAACTCGGATTTTGGTCCGAAGAGGAAGCAGCTGGCTGAAGAGCCACAAATGATGTTAGATCAGAAGTATTACGCCTGCGAATATCTGCAGTGCCCGTATCATGATTATCGCCTAGGCTTTCTCGACATAACTGCAAGAAACAATCATCAGTTGAATTGTCCATACCGGAATAATTCTTCACAAGTTCTGGGAATGTCAAGCTTTCAGCTTCACAATGAGAAACCTGTGGACTTCTCTCTACCCATTGCTCAACCACCAACACCAGCAATTCAACGACCAGTGAACCAGTCAAGTGGGTTTGATGCTTTGGGACTTGGAAATCAACAGCAGGTGAACCAATCAAGTAGGTTCGATGCTTCGGGACTTGGAAATCAACAGCAGGTGAACCAGTCAAATAGGTTCGATGCTTCGAGAATTGGAATTCAACAACCAGTGAACCAGTCAGGTAGGTTTGATTCTTCGGGGCTTGGAATTGCTGAGGATGGGCAGAAAATGATATCCGAGCTTATGTCATTCTATGATAGTAATATACAGCAAAACAAGAACTGCAATCCTGGAAACCTAAATGATGTAGATGATTGCAATCAGCAGCAGGCTAACTTTCAATTTCCAATGAATGATAACTTATATGGTCAAGGGCTGGACATAGGACGCAACATGAACATGTCGGAACAGTCCCCAATGCCTATGCTTCATCCAGATTTTTCATCCCCGGAAGTTCAGTTTGATCAGTTGATGGCATTTGATTCTCCATTTGGTAACAATTCCAACGAAGATGTCGACATAAGATTTGGCTCCCCCTTGCACTTGTCACCTGTTGGTTATAATGTTATGGACCCGCCTCTGAACCAAGACCCCTGGTTCCCATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000160 GO:0000976 GO:0001067 GO:0001666 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0006950 GO:0006952 GO:0007154 GO:0007165 GO:0008150 GO:0009605 GO:0009607 GO:0009617 GO:0009628 GO:0009719 GO:0009723 GO:0009725 GO:0009743 GO:0009755 GO:0009756 GO:0009873 GO:0009889 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010039 GO:0010182 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0023052 GO:0031323 GO:0031326 GO:0032870 GO:0035556 GO:0036293 GO:0042221 GO:0042742 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044212 GO:0044424 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051252 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0065007 GO:0070482 GO:0070887 GO:0071241 GO:0071248 GO:0071281 GO:0071310 GO:0071322 GO:0071369 GO:0071495 GO:0080090 GO:0097159 GO:0098542 GO:0140110 GO:1901363 GO:1901700 GO:1901701 GO:1903506 GO:1990837 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

ko04016 ko04075 map04016 map04075

Pfam Domains

Protein Families

EIN3

Protein Analysis

660

Amino Acids

74.44

Weight (kDa)

5.11

Isoelectric Point (pI)

58.19

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
EIN3_DNA-bd	PF04873	47 - 294	1.1e-131	Ethylene insensitive 3, DNA-binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0001963)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G27050 AT3G20770
fragaria_vesca	FvH4_1g28720 FvH4_1g28720 FvH4_7g04940 FvH4_7g04940 FvH4_7g04940 FvH4_7g04940 FvH4_7g04940
malus_domestica	MD02G1266200.v1.1 MD02G1266300.v1.1 MD07G1053500.v1.1 MD07G1053800.v1.1 MD08G1245800.v1.1 MD15G1441000.v1.1
prunus_persica	Prupe.2G058400_v2.0.a1 Prupe.2G058500_v2.0.a1 Prupe.2G070300_v2.0.a1
pyrus_communis	pycom02g22750 pycom02g22760 pycom07g04140 pycom07g04160 pycom08g21330 pycom15g38880
rosa_chinensis	RchiOBHm_Chr1g0331011 RchiOBHm_Chr5g0049961
rosa_laevigata	RLG00000029759 RLG00000034672
rosa_multiflora	Rmu_sc0000333.1_g000038 Rmu_sc0013622.1_g000002 Rmu_sc0013622.1_g000003
rosa_roxburghii	Rroxscaffold_1G00031290 Rroxscaffold_4G00319550
rosa_rugosa	Rorug01G0091600 Rorug05G0255200
rosa_samantha	Rh1AG113300 Rh1BG087200 Rh1CG110200 Rh1DG118900 Rh5AG329100 Rh5BG339600 Rh5CG365600 Rh5DG352200
rosa_wichuraiana	Rw1G009240 Rw5G031070

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	869, 1935
AarI	CACCTGC	3 cut(s)	1063, 1429, 1480
Acc36I	ACCTGC	3 cut(s)	1063, 1429, 1480
Acc65I	GGTACC	1 cut(s)	1078
AccB1I	GGYRCC	2 cut(s)	380, 1078
AccB7I	CCANNNNNTGG	2 cut(s)	377, 929
AccI	GTMKAC	1 cut(s)	1890
AccII	CGCG	1 cut(s)	256
AciI	CCGC	4 cut(s)	256, 665, 793, 1949
AclWI	GGATC	2 cut(s)	693, 784
AcoI	YGGCCR	1 cut(s)	662
AcsI	RAATTY	1 cut(s)	1530
AcuI	CTGAAG	4 cut(s)	200, 481, 743, 1167
AcyI	GRCGYC	1 cut(s)	381
AfaI	GTAC	2 cut(s)	961, 1080
AfiI	CCNNNNNNNGG	3 cut(s)	362, 377, 929
AflIII	ACRYGT	2 cut(s)	768, 1773
AgsI	TTSAA	7 cut(s)	474, 980, 1007, 1264, 1382, 1535, 1715
AhlI	ACTAGT	1 cut(s)	962
AjnI	CCWGG	5 cut(s)	478, 681, 873, 1663, 1967
AleI	CACNNNNGTG	1 cut(s)	755
Alw21I	GWGCWC	2 cut(s)	537, 1101
Alw26I	GTCTC	1 cut(s)	948
AlwI	GGATC	2 cut(s)	693, 784
AoxI	GGCC	4 cut(s)	436, 638, 662, 888
ApeKI	GCWGC	5 cut(s)	74, 443, 577, 1138, 1699
ApoI	RAATTY	1 cut(s)	1530
ArsI	GACNNNNNNTTYG	2 cut(s)	1883, 1915
Asp718I	GGTACC	1 cut(s)	1078
AspA2I	CCTAGG	1 cut(s)	1222
AspLEI	GCGC	3 cut(s)	256, 383, 851
AspS9I	GGNCC	4 cut(s)	437, 889, 1124, 1945
AsuC2I	CCSGG	3 cut(s)	440, 892, 1823
AsuHPI	GGTGA	4 cut(s)	16, 1453, 1504, 1914
AsuNHI	GCTAGC	1 cut(s)	859
AvaII	GGWCC	2 cut(s)	1124, 1945
AvrII	CCTAGG	1 cut(s)	1222
AxyI	CCTNAGG	1 cut(s)	1083
BaeGI	GKGCMC	2 cut(s)	280, 1205
BanI	GGYRCC	2 cut(s)	380, 1078
BanII	GRGCYC	1 cut(s)	537
BbsI	GAAGAC	1 cut(s)	498
Bbv12I	GWGCWC	2 cut(s)	537, 1101
BbvCI	CCTCAGC	1 cut(s)	1587
BbvI	GCAGC	5 cut(s)	86, 430, 589, 1150, 1711
BccI	CCATC	5 cut(s)	64, 278, 307, 1586, 1840
BceAI	ACGGC	1 cut(s)	768
BcgI	CGANNNNNNTGC	2 cut(s)	1223, 1257
BciT130I	CCWGG	5 cut(s)	480, 683, 875, 1665, 1969
BclI	TGATCA	1 cut(s)	1837
BcnI	CCSGG	3 cut(s)	440, 892, 1823
BcoDI	GTCTC	1 cut(s)	948
BcuI	ACTAGT	1 cut(s)	962
BfaI	CTAG	3 cut(s)	860, 963, 1223
BfmI	CTRYAG	1 cut(s)	1196
BfoI	RGCGCY	2 cut(s)	384, 852
BfuAI	ACCTGC	3 cut(s)	1063, 1429, 1480
BisI	GCNGC	6 cut(s)	75, 444, 578, 665, 1139, 1700
BlnI	CCTAGG	1 cut(s)	1222
BlsI	GCNGC	6 cut(s)	76, 445, 579, 666, 1140, 1701
Bme1390I	CCNGG	8 cut(s)	440, 480, 683, 875, 892, 1665, 1823, 1969
Bme18I	GGWCC	2 cut(s)	1124, 1945
BmgT120I	GGNCC	4 cut(s)	437, 889, 1124, 1945
BmiI	GGNNCC	6 cut(s)	54, 382, 1080, 1906, 1947, 1973
BmrFI	CCNGG	8 cut(s)	440, 480, 683, 875, 892, 1665, 1823, 1969
BmsI	GCATC	6 cut(s)	61, 518, 564, 1402, 1453, 1504
BmtI	GCTAGC	1 cut(s)	863
BpiI	GAAGAC	1 cut(s)	498
BplI	GAGNNNNNCTC	4 cut(s)	928, 960, 1097, 1129
BpmI	CTGGAG	1 cut(s)	63
Bpu10I	CCTNAGC	3 cut(s)	330, 840, 1587
BpuEI	CTTGAG	1 cut(s)	167
BpuMI	CCSGG	3 cut(s)	440, 892, 1823
BsaBI	GATNNNNATC	1 cut(s)	1692
BsaHI	GRCGYC	1 cut(s)	381
BsaJI	CCNNGG	7 cut(s)	478, 479, 632, 681, 1222, 1821, 1967
BsaWI	WCCGGW	1 cut(s)	1275
BsaXI	ACNNNNNCTCC	4 cut(s)	519, 549, 660, 690
Bsc4I	CCNNNNNNNGG	3 cut(s)	362, 377, 929
Bse1I	ACTGG	8 cut(s)	371, 649, 1060, 1388, 1396, 1498, 1541, 1549
Bse21I	CCTNAGG	1 cut(s)	1083
Bse3DI	GCAATG	1 cut(s)	1353
Bse8I	GATNNNNATC	1 cut(s)	1692
BseBI	CCWGG	5 cut(s)	480, 683, 875, 1665, 1969
BseDI	CCNNGG	7 cut(s)	478, 479, 632, 681, 1222, 1821, 1967
BseGI	GGATG	7 cut(s)	76, 139, 162, 509, 1597, 1804, 1817
BseJI	GATNNNNATC	1 cut(s)	1692
BseLI	CCNNNNNNNGG	3 cut(s)	362, 377, 929
BseMI	GCAATG	1 cut(s)	1353
BseMII	CTCAG	3 cut(s)	344, 1097, 1578
BseNI	ACTGG	8 cut(s)	371, 649, 1060, 1388, 1396, 1498, 1541, 1549
BseRI	GAGGAG	4 cut(s)	29, 122, 507, 618
BseSI	GKGCMC	2 cut(s)	280, 1205
BseXI	GCAGC	5 cut(s)	86, 430, 589, 1150, 1711
BsgI	GTGCAG	1 cut(s)	522
Bsh1236I	CGCG	1 cut(s)	256
BshFI	GGCC	4 cut(s)	438, 640, 664, 890
BshNI	GGYRCC	2 cut(s)	380, 1078
BsiHKAI	GWGCWC	2 cut(s)	537, 1101
BsiSI	CCGG	4 cut(s)	440, 892, 1276, 1823
BslFI	GGGAC	3 cut(s)	1434, 1485, 1771
BslI	CCNNNNNNNGG	3 cut(s)	362, 377, 929
BsmAI	GTCTC	1 cut(s)	948
BsmFI	GGGAC	3 cut(s)	1434, 1485, 1771
BsmI	GAATGC	1 cut(s)	177
BsnI	GGCC	4 cut(s)	438, 640, 664, 890
Bsp1286I	GDGCHC	4 cut(s)	280, 537, 1101, 1205
BspACI	CCGC	4 cut(s)	256, 665, 793, 1949
BspANI	GGCC	4 cut(s)	438, 640, 664, 890
BspCNI	CTCAG	3 cut(s)	343, 1096, 1579
BspFNI	CGCG	1 cut(s)	256
BspHI	TCATGA	2 cut(s)	715, 1210
BspLI	GGNNCC	6 cut(s)	54, 382, 1080, 1906, 1947, 1973
BspMAI	CTGCAG	1 cut(s)	1200
BspMI	ACCTGC	3 cut(s)	1063, 1429, 1480
BspOI	GCTAGC	1 cut(s)	863
BspPI	GGATC	2 cut(s)	693, 784
BspQI	GCTCTTC	1 cut(s)	1143
BspT107I	GGYRCC	2 cut(s)	380, 1078
BsrDI	GCAATG	1 cut(s)	1353
BsrI	ACTGG	8 cut(s)	371, 649, 1060, 1388, 1396, 1498, 1541, 1549
BssECI	CCNNGG	7 cut(s)	478, 479, 632, 681, 1222, 1821, 1967
BssNI	GRCGYC	1 cut(s)	381
BssT1I	CCWWGG	2 cut(s)	632, 1222
Bst2UI	CCWGG	5 cut(s)	480, 683, 875, 1665, 1969
Bst4CI	ACNGT	2 cut(s)	103, 1785
Bst6I	CTCTTC	4 cut(s)	146, 176, 1124, 1143
BstACI	GRCGYC	1 cut(s)	381
BstC8I	GCNNGC	6 cut(s)	399, 797, 861, 1143, 1186, 1704
BstDEI	CTNAG	6 cut(s)	201, 330, 453, 840, 1083, 1587
BstENI	CCTNNNNNAGG	1 cut(s)	360
BstF5I	GGATG	7 cut(s)	76, 139, 162, 509, 1597, 1804, 1817
BstFNI	CGCG	1 cut(s)	256
BstH2I	RGCGCY	2 cut(s)	384, 852
BstHHI	GCGC	3 cut(s)	256, 383, 851
BstMAI	GTCTC	1 cut(s)	948
BstMWI	GCNNNNNNNGC	1 cut(s)	772
BstNI	CCWGG	5 cut(s)	480, 683, 875, 1665, 1969
BstNSI	RCATGY	2 cut(s)	772, 1777
BstSCI	CCNGG	8 cut(s)	438, 478, 681, 873, 890, 1663, 1821, 1967
BstSFI	CTRYAG	1 cut(s)	1196
BstSLI	GKGCMC	2 cut(s)	280, 1205
BstUI	CGCG	1 cut(s)	256
BstV1I	GCAGC	5 cut(s)	86, 430, 589, 1150, 1711
BstV2I	GAAGAC	1 cut(s)	498
BstX2I	RGATCY	1 cut(s)	789
BstXI	CCANNNNNNTGG	1 cut(s)	1403
BstYI	RGATCY	1 cut(s)	789
Bsu36I	CCTNAGG	1 cut(s)	1083
BsuRI	GGCC	4 cut(s)	438, 640, 664, 890
BtsCI	GGATG	7 cut(s)	76, 139, 162, 509, 1597, 1804, 1817
BtsI	GCAGTG	3 cut(s)	510, 580, 1205
BtsIMutI	CAGTG	9 cut(s)	378, 505, 510, 580, 695, 961, 1205, 1395, 1548
BveI	ACCTGC	3 cut(s)	1063, 1429, 1480
Cac8I	GCNNGC	6 cut(s)	399, 797, 861, 1143, 1186, 1704
CciI	TCATGA	2 cut(s)	715, 1210
CfoI	GCGC	3 cut(s)	256, 383, 851
Cfr13I	GGNCC	4 cut(s)	437, 889, 1124, 1945
CseI	GACGC	2 cut(s)	224, 1770
Csp6I	GTAC	2 cut(s)	960, 1079
CviAII	CATG	8 cut(s)	86, 402, 716, 769, 1211, 1768, 1774, 1977
CviQI	GTAC	2 cut(s)	960, 1079
DdeI	CTNAG	6 cut(s)	201, 330, 453, 840, 1083, 1587
DinI	GGCGCC	1 cut(s)	382
EaeI	YGGCCR	1 cut(s)	662
Eam1104I	CTCTTC	4 cut(s)	146, 176, 1124, 1143
EarI	CTCTTC	4 cut(s)	146, 176, 1124, 1143
Ecl136II	GAGCTC	1 cut(s)	535
Eco130I	CCWWGG	2 cut(s)	632, 1222
Eco24I	GRGCYC	1 cut(s)	537
Eco32I	GATATC	1 cut(s)	1608
Eco47I	GGWCC	2 cut(s)	1124, 1945
Eco53kI	GAGCTC	1 cut(s)	535
Eco57I	CTGAAG	4 cut(s)	200, 481, 743, 1167
Eco81I	CCTNAGG	1 cut(s)	1083
EcoICRI	GAGCTC	1 cut(s)	535
EcoNI	CCTNNNNNAGG	1 cut(s)	360
EcoRI	GAATTC	1 cut(s)	1530
EcoRII	CCWGG	5 cut(s)	478, 681, 873, 1663, 1967
EcoRV	GATATC	1 cut(s)	1608
EcoT14I	CCWWGG	2 cut(s)	632, 1222
EcoT38I	GRGCYC	1 cut(s)	537
EgeI	GGCGCC	1 cut(s)	382
EheI	GGCGCC	1 cut(s)	382
ErhI	CCWWGG	2 cut(s)	632, 1222
FaeI	CATG	8 cut(s)	89, 405, 719, 772, 1214, 1771, 1777, 1980
FaqI	GGGAC	3 cut(s)	1434, 1485, 1771
FatI	CATG	8 cut(s)	85, 401, 715, 768, 1210, 1767, 1773, 1976
FauI	CCCGC	1 cut(s)	1956
FbaI	TGATCA	1 cut(s)	1837
FblI	GTMKAC	1 cut(s)	1890
Fnu4HI	GCNGC	6 cut(s)	75, 444, 578, 665, 1139, 1700
FokI	GGATG	7 cut(s)	83, 146, 169, 496, 1604, 1791, 1804
FriOI	GRGCYC	1 cut(s)	537
Fsp4HI	GCNGC	6 cut(s)	75, 444, 578, 665, 1139, 1700
FspBI	CTAG	3 cut(s)	860, 963, 1223
GlaI	GCGC	3 cut(s)	255, 382, 850
GluI	GCNGC	6 cut(s)	75, 444, 578, 665, 1139, 1700
GsuI	CTGGAG	1 cut(s)	63
HaeII	RGCGCY	2 cut(s)	384, 852
HaeIII	GGCC	4 cut(s)	438, 640, 664, 890
HapII	CCGG	4 cut(s)	440, 892, 1276, 1823
HgaI	GACGC	2 cut(s)	224, 1770
HhaI	GCGC	3 cut(s)	256, 383, 851
Hin1I	GRCGYC	1 cut(s)	381
Hin1II	CATG	8 cut(s)	89, 405, 719, 772, 1214, 1771, 1777, 1980
Hin6I	GCGC	3 cut(s)	254, 381, 849
HinP1I	GCGC	3 cut(s)	254, 381, 849
HincII	GTYRAC	2 cut(s)	220, 1891
HindII	GTYRAC	2 cut(s)	220, 1891
HindIII	AAGCTT	2 cut(s)	797, 1309
HinfI	GANTC	4 cut(s)	288, 941, 1565, 1856
HpaII	CCGG	4 cut(s)	440, 892, 1276, 1823
HphI	GGTGA	4 cut(s)	16, 1453, 1504, 1914
Hpy166II	GTNNAC	9 cut(s)	220, 557, 917, 1339, 1393, 1444, 1495, 1546, 1891
Hpy188I	TCNGA	8 cut(s)	388, 723, 1117, 1128, 1173, 1612, 1780, 1956
Hpy8I	GTNNAC	9 cut(s)	220, 557, 917, 1339, 1393, 1444, 1495, 1546, 1891
HpyAV	CCTTC	7 cut(s)	59, 206, 403, 610, 721, 730, 974
HpyCH4III	ACNGT	2 cut(s)	103, 1785
HpyCH4IV	ACGT	1 cut(s)	601
HpyF10VI	GCNNNNNNNGC	1 cut(s)	772
HpyF3I	CTNAG	6 cut(s)	201, 330, 453, 840, 1083, 1587
HpySE526I	ACGT	1 cut(s)	601
Hsp92I	GRCGYC	1 cut(s)	381
Hsp92II	CATG	8 cut(s)	89, 405, 719, 772, 1214, 1771, 1777, 1980
HspAI	GCGC	3 cut(s)	254, 381, 849
KasI	GGCGCC	1 cut(s)	380
KpnI	GGTACC	1 cut(s)	1082
Ksp22I	TGATCA	1 cut(s)	1837
LguI	GCTCTTC	1 cut(s)	1143
LmnI	GCTCC	7 cut(s)	58, 82, 532, 540, 631, 1049, 1910
Lsp1109I	GCAGC	5 cut(s)	86, 430, 589, 1150, 1711
LweI	GCATC	6 cut(s)	61, 518, 564, 1402, 1453, 1504
MaeI	CTAG	3 cut(s)	860, 963, 1223
MaeII	ACGT	1 cut(s)	601
MaeIII	GTNAC	4 cut(s)	270, 647, 1868, 1920
MflI	RGATCY	1 cut(s)	789
MhlI	GDGCHC	4 cut(s)	280, 537, 1101, 1205
MluCI	AATT	9 cut(s)	348, 1264, 1282, 1377, 1524, 1530, 1581, 1715, 1873
Mly113I	GGCGCC	1 cut(s)	381
MlyI	GAGTC	1 cut(s)	950
MmeI	TCCRAC	3 cut(s)	591, 1758, 1902
MseI	TTAA	3 cut(s)	762, 1032, 1089
MslI	CAYNNNNRTG	2 cut(s)	755, 1772
MspA1I	CMGCKG	1 cut(s)	1141
MspI	CCGG	4 cut(s)	440, 892, 1276, 1823
MspR9I	CCNGG	8 cut(s)	440, 480, 683, 875, 892, 1665, 1823, 1969
Mva1269I	GAATGC	1 cut(s)	177
MvaI	CCWGG	5 cut(s)	480, 683, 875, 1665, 1969
MvnI	CGCG	1 cut(s)	256
MwoI	GCNNNNNNNGC	1 cut(s)	772
NarI	GGCGCC	1 cut(s)	381
NciI	CCSGG	3 cut(s)	440, 892, 1823
NheI	GCTAGC	1 cut(s)	859
NlaIII	CATG	8 cut(s)	89, 405, 719, 772, 1214, 1771, 1777, 1980
NlaIV	GGNNCC	6 cut(s)	54, 382, 1080, 1906, 1947, 1973
NmuCI	GTSAC	2 cut(s)	270, 1920
NspI	RCATGY	2 cut(s)	772, 1777
OliI	CACNNNNGTG	1 cut(s)	755
PagI	TCATGA	2 cut(s)	715, 1210
PaqCI	CACCTGC	3 cut(s)	1063, 1429, 1480
PasI	CCCWGGG	1 cut(s)	479
PciI	ACATGT	2 cut(s)	768, 1773
PciSI	GCTCTTC	1 cut(s)	1143
PcsI	WCGNNNNNNNCGW	1 cut(s)	1887
PctI	GAATGC	1 cut(s)	177
PfeI	GAWTC	3 cut(s)	288, 1565, 1856
PflFI	GACNNNGTC	1 cut(s)	234
PflMI	CCANNNNNTGG	2 cut(s)	377, 929
PfoI	TCCNGGA	1 cut(s)	1663
PkrI	GCNGC	6 cut(s)	76, 445, 579, 666, 1140, 1701
PleI	GAGTC	1 cut(s)	949
PluTI	GGCGCC	1 cut(s)	384
PpsI	GAGTC	1 cut(s)	949
PscI	ACATGT	2 cut(s)	768, 1773
PsiI	TTATAA	2 cut(s)	869, 1935
Psp124BI	GAGCTC	1 cut(s)	537
Psp6I	CCWGG	5 cut(s)	478, 681, 873, 1663, 1967
PspGI	CCWGG	5 cut(s)	478, 681, 873, 1663, 1967
PspN4I	GGNNCC	6 cut(s)	54, 382, 1080, 1906, 1947, 1973
PspPI	GGNCC	4 cut(s)	437, 889, 1124, 1945
PstI	CTGCAG	1 cut(s)	1200
PsuI	RGATCY	1 cut(s)	789
PsyI	GACNNNGTC	1 cut(s)	234
PvuII	CAGCTG	1 cut(s)	1141
RsaI	GTAC	2 cut(s)	961, 1080
RsaNI	GTAC	2 cut(s)	960, 1079
RseI	CAYNNNNRTG	2 cut(s)	755, 1772
SacI	GAGCTC	1 cut(s)	537
SalI	GTCGAC	1 cut(s)	1889
SapI	GCTCTTC	1 cut(s)	1143
SaqAI	TTAA	3 cut(s)	762, 1032, 1089
SatI	GCNGC	6 cut(s)	75, 444, 578, 665, 1139, 1700
Sau96I	GGNCC	4 cut(s)	437, 889, 1124, 1945
SchI	GAGTC	1 cut(s)	950
ScrFI	CCNGG	8 cut(s)	440, 480, 683, 875, 892, 1665, 1823, 1969
SduI	GDGCHC	4 cut(s)	280, 537, 1101, 1205
SfaNI	GCATC	6 cut(s)	61, 518, 564, 1402, 1453, 1504
SfcI	CTRYAG	1 cut(s)	1196
SfoI	GGCGCC	1 cut(s)	382
SinI	GGWCC	2 cut(s)	1124, 1945
SmiMI	CAYNNNNRTG	2 cut(s)	755, 1772
SmlI	CTYRAG	1 cut(s)	146
SmoI	CTYRAG	1 cut(s)	146
SpeI	ACTAGT	1 cut(s)	962
Sse9I	AATT	9 cut(s)	348, 1264, 1282, 1377, 1524, 1530, 1581, 1715, 1873
SsiI	CCGC	4 cut(s)	256, 665, 793, 1949
SspDI	GGCGCC	1 cut(s)	380
SspMI	CTAG	3 cut(s)	860, 963, 1223
SstI	GAGCTC	1 cut(s)	537
StyD4I	CCNGG	8 cut(s)	438, 478, 681, 873, 890, 1663, 1821, 1967
StyI	CCWWGG	2 cut(s)	632, 1222
TaaI	ACNGT	2 cut(s)	103, 1785
TaiI	ACGT	1 cut(s)	604
TaqI	TCGA	7 cut(s)	169, 1104, 1233, 1461, 1512, 1520, 1890
TasI	AATT	9 cut(s)	348, 1264, 1282, 1377, 1524, 1530, 1581, 1715, 1873
TauI	GCSGC	1 cut(s)	667
TfiI	GAWTC	3 cut(s)	288, 1565, 1856
Tru1I	TTAA	3 cut(s)	762, 1032, 1089
Tru9I	TTAA	3 cut(s)	762, 1032, 1089
TscAI	CASTG	9 cut(s)	378, 505, 510, 587, 695, 961, 1205, 1395, 1548
TseFI	GTSAC	2 cut(s)	270, 1920
TseI	GCWGC	5 cut(s)	74, 443, 577, 1138, 1699
Tsp45I	GTSAC	2 cut(s)	270, 1920
TspDTI	ATGAA	8 cut(s)	102, 138, 504, 1030, 1739, 1784, 1793, 1806
TspRI	CASTG	9 cut(s)	378, 505, 510, 587, 695, 961, 1205, 1395, 1548
Tth111I	GACNNNGTC	1 cut(s)	234
Van91I	CCANNNNNTGG	2 cut(s)	377, 929
VpaK11BI	GGWCC	2 cut(s)	1124, 1945
XagI	CCTNNNNNAGG	1 cut(s)	360
XapI	RAATTY	1 cut(s)	1530
XceI	RCATGY	2 cut(s)	772, 1777
XmaJI	CCTAGG	1 cut(s)	1222
XmiI	GTMKAC	1 cut(s)	1890
XspI	CTAG	3 cut(s)	860, 963, 1223

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.