Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr2

Orthogroup ID

OG0001963

Physical Location & Seq

Genomic Coordinates Reverse (-)

20947823 .. 20949667

Gene Structure 1845 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom02g22760.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1845 bp

ATGGCGATCATTGAGGAGTTGGAGTTCATTGAGGACCTGGACTTTCTTTTGGCTCTCCCTGAGGAAGGTGAGGCAGCCCCAGAGCATGAACCAGAGGCAACAGTGGAGGAGGATGATAGTGACGACGAATTGGATGTTAATGAGCTTGAGAGGAGGGTGTGGAAATATAGAATGCGACTGAAGCGGCTTAAAGAGCAAAATAAGGGAACAGAAGGAGCTGACAATGCTAGGCAGTGTCAGCCACAGGAACAAGCTCGGAGGAAGAGGATGTCACGGGCACAAGATGGAATCCTGAAATATATGTTAAAAATGATGGAAGTTTGTAAAGCCAAGGGTTTTGTTTATGGGATTATCCCCGAAAATGGTAAACCAGTGACTGGTGCGTCTGACAGTCTTCGCGAATGGTGGAAGGAAAAAGTAAGATTTGACCATAATGGCCCTGCTGCAGTTTCTAAGTATCTGGCTGAACATCCAATCCCTGGGAAGAATGAAGAGTCCCATGCAGTACCATCCACTCCTCACAACTTACAGGAGCTCCATGATGCCACTCTTGGTTCACTTTTGTCGGCTTTGATGCAGCACTGCCATCCACCCCAAAGACGTTTCCCGTTGGAGAAGGGTGTTACCCCACCTTGGTGGCCCACTGGCAATGAGGAATGGTGGACTCAATTGAATCTGCGCAAGGATCAGGGTCCTCACCCATACAGGAAGCCTCATGATCTGAAGAAGGCTTGGAAGGTGAGTGTTCTCGCGGCTGTGATAAAGCACATGTCACCCGATATTGCCAGGATCCGGAAGCTTGTTCATCGGTCTAAATATTTGCAGGACAAAATGTCTGCTAAGGAGACCACTATTTGGCTAGCCATCCTTAACCAGGAGGAGAATTTGGCCCAGAGGTTATATCCTGATGGATGTACACCTCCATCATCTTCTGGGAGTGGGTCTAGTTTTGCAATCAGTGGTACCAGTGATTATGATGTTGAAGTGGTTGATGATGAAGAGAATTATGAAGAAGAGGACTGCCAACCTCATTTTAATCACTTAAATATTGGAACATCTGGACAAAGAGATGGACTGATGCATCATTTTATGTCAGAGCTGACTGAGACCGATTCAGACTCTGGTCAAAAGAGGAAGCAGCTGCCTGAAGAGCCACAAATGATGTTAAATCATCAGGGTTACACCTGCGAATATCTGGATTGCCCGTATCATGATTATCACCTAGGCTTTCGCAACATAACTGCAAGGAATAATCACCAGTTGACTTGTTCTTACAGGAATAATTCTTCACAAGTTGGAATGTCAAGCGCTCATCCTAACGGTGACAAACTTGTGGGGTCCTTACCCAGTCCTCAACCTAATCCAGCTATTCAACAACCAGTGAACCAGACAAGTAGTTTTAATGCTTCCGGACTTGAACCTGCTGGGGATGAGCAGAATATGATATCGGAGTTTATGTCATTCTGTGATGGTAATACTCAGCAGAACAAGAACTCGGATCCTGGAAGCCTCGATGTTATAGAAAACCACAACCAGCATCAGGTGAATTATCAATTTCCAATGGGTGATAACTTCTTTGATCAAAGGTTGGTTGTAGGACCCAACATGTCTGCAGCGTCACCTATGCCTATGCCTATGCCTATGCCTATGCTTCCTCCAGCTACTCCAGCTCCTCCATCTCTCCCATCCACAGAATTTCAGTTTGATCCGTGCAACTTGTTTGATTCTCCATTTGGTAATCTTCCCGATAATACTGACAACTTAGGATTTGGCACCAACTTGCCCCGAGATGACTATAGTCTTGACTCGATGCTGAAACAAGATCCATTCTGGTTGTGTCGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000160 GO:0000976 GO:0001067 GO:0001666 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0006950 GO:0006952 GO:0007154 GO:0007165 GO:0008150 GO:0009605 GO:0009607 GO:0009617 GO:0009628 GO:0009719 GO:0009723 GO:0009725 GO:0009743 GO:0009755 GO:0009756 GO:0009873 GO:0009889 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010039 GO:0010182 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0023052 GO:0031323 GO:0031326 GO:0032870 GO:0035556 GO:0036293 GO:0042221 GO:0042742 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044212 GO:0044424 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051252 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0065007 GO:0070482 GO:0070887 GO:0071241 GO:0071248 GO:0071281 GO:0071310 GO:0071322 GO:0071369 GO:0071495 GO:0080090 GO:0097159 GO:0098542 GO:0140110 GO:1901363 GO:1901700 GO:1901701 GO:1903506 GO:1990837 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

ko04016 ko04075 map04016 map04075

Pfam Domains

Protein Families

EIN3

Protein Analysis

615

Amino Acids

69.54

Weight (kDa)

5.12

Isoelectric Point (pI)

58.86

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
EIN3_DNA-bd	PF04873	48 - 294	1.6e-121	Ethylene insensitive 3, DNA-binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0001963)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G27050 AT3G20770
fragaria_vesca	FvH4_1g28720 FvH4_1g28720 FvH4_7g04940 FvH4_7g04940 FvH4_7g04940 FvH4_7g04940 FvH4_7g04940
malus_domestica	MD02G1266200.v1.1 MD02G1266300.v1.1 MD07G1053500.v1.1 MD07G1053800.v1.1 MD08G1245800.v1.1 MD15G1441000.v1.1
prunus_persica	Prupe.2G058400_v2.0.a1 Prupe.2G058500_v2.0.a1 Prupe.2G070300_v2.0.a1
pyrus_communis	pycom02g22750 pycom02g22760 pycom07g04140 pycom07g04160 pycom08g21330 pycom15g38880
rosa_chinensis	RchiOBHm_Chr1g0331011 RchiOBHm_Chr5g0049961
rosa_laevigata	RLG00000029759 RLG00000034672
rosa_multiflora	Rmu_sc0000333.1_g000038 Rmu_sc0013622.1_g000002 Rmu_sc0013622.1_g000003
rosa_roxburghii	Rroxscaffold_1G00031290 Rroxscaffold_4G00319550
rosa_rugosa	Rorug01G0091600 Rorug05G0255200
rosa_samantha	Rh1AG113300 Rh1BG087200 Rh1CG110200 Rh1DG118900 Rh5AG329100 Rh5BG339600 Rh5CG365600 Rh5DG352200
rosa_wichuraiana	Rw1G009240 Rw5G031070

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1193
AasI	GACNNNNNNGTC	1 cut(s)	382
Acc16I	TGCGCA	1 cut(s)	680
Acc36I	ACCTGC	2 cut(s)	1193, 1429
Acc65I	GGTACC	1 cut(s)	962
AccB1I	GGYRCC	2 cut(s)	962, 1772
AccB7I	CCANNNNNTGG	3 cut(s)	377, 479, 855
AccII	CGCG	2 cut(s)	399, 752
AccIII	TCCGGA	2 cut(s)	792, 1409
AciI	CCGC	2 cut(s)	184, 752
AclWI	GGATC	7 cut(s)	693, 784, 797, 1493, 1506, 1700, 1817
AcsI	RAATTY	2 cut(s)	883, 1694
AcuI	CTGAAG	3 cut(s)	200, 743, 1167
AfaI	GTAC	3 cut(s)	507, 916, 964
AfeI	AGCGCT	1 cut(s)	1309
AfiI	CCNNNNNNNGG	9 cut(s)	65, 362, 377, 479, 633, 792, 855, 874, 1540
AflIII	ACRYGT	2 cut(s)	768, 1605
AgsI	TTSAA	4 cut(s)	673, 983, 1373, 1418
AhdI	GACNNNNNGTC	1 cut(s)	832
AjnI	CCWGG	5 cut(s)	36, 478, 785, 873, 1501
AleI	CACNNNNGTG	1 cut(s)	634
AloI	GAACNNNNNNTCC	4 cut(s)	8, 40, 459, 491
Alw21I	GWGCWC	1 cut(s)	537
Alw26I	GTCTC	2 cut(s)	839, 1100
AlwI	GGATC	7 cut(s)	693, 784, 797, 1493, 1506, 1700, 1817
AlwNI	CAGNNNCTG	2 cut(s)	377, 1121
Ama87I	CYCGRG	1 cut(s)	1785
Aor13HI	TCCGGA	2 cut(s)	792, 1409
Aor51HI	AGCGCT	1 cut(s)	1309
AoxI	GGCC	3 cut(s)	436, 638, 888
ApeKI	GCWGC	6 cut(s)	74, 443, 577, 1138, 1141, 1613
ApoI	RAATTY	2 cut(s)	883, 1694
Asp718I	GGTACC	1 cut(s)	962
AspA2I	CCTAGG	1 cut(s)	1222
AspLEI	GCGC	2 cut(s)	681, 1310
AspS9I	GGNCC	7 cut(s)	34, 437, 639, 692, 889, 1338, 1598
AsuNHI	GCTAGC	1 cut(s)	859
AvaI	CYCGRG	1 cut(s)	1785
AvaII	GGWCC	4 cut(s)	34, 692, 1338, 1598
AvrII	CCTAGG	1 cut(s)	1222
AxyI	CCTNAGG	1 cut(s)	60
BaeGI	GKGCMC	1 cut(s)	280
BamHI	GGATCC	2 cut(s)	789, 1498
BanI	GGYRCC	2 cut(s)	962, 1772
BanII	GRGCYC	1 cut(s)	537
BbsI	GAAGAC	1 cut(s)	386
Bbv12I	GWGCWC	1 cut(s)	537
BbvI	GCAGC	6 cut(s)	86, 430, 589, 1128, 1150, 1625
BciT130I	CCWGG	5 cut(s)	38, 480, 787, 875, 1503
BclI	TGATCA	1 cut(s)	1579
BcoDI	GTCTC	2 cut(s)	839, 1100
BfaI	CTAG	4 cut(s)	228, 860, 945, 1223
BfmI	CTRYAG	3 cut(s)	444, 1611, 1795
BfoI	RGCGCY	1 cut(s)	1311
BfuAI	ACCTGC	2 cut(s)	1193, 1429
BisI	GCNGC	8 cut(s)	75, 185, 444, 578, 753, 1139, 1142, 1614
BlnI	CCTAGG	1 cut(s)	1222
BlsI	GCNGC	8 cut(s)	76, 186, 445, 579, 754, 1140, 1143, 1615
Bme1390I	CCNGG	5 cut(s)	38, 480, 787, 875, 1503
Bme18I	GGWCC	4 cut(s)	34, 692, 1338, 1598
BmeRI	GACNNNNNGTC	1 cut(s)	832
BmeT110I	CYCGRG	1 cut(s)	1785
BmgT120I	GGNCC	7 cut(s)	34, 437, 639, 692, 889, 1338, 1598
BmiI	GGNNCC	7 cut(s)	693, 791, 964, 1339, 1500, 1600, 1774
BmrFI	CCNGG	5 cut(s)	38, 480, 787, 875, 1503
BmrI	ACTGGG	1 cut(s)	1341
BmsI	GCATC	6 cut(s)	532, 564, 1068, 1090, 1546, 1800
BmtI	GCTAGC	1 cut(s)	863
BmuI	ACTGGG	1 cut(s)	1341
BoxI	GACNNNNGTC	2 cut(s)	1122, 1797
BpiI	GAAGAC	1 cut(s)	386
BpmI	CTGGAG	2 cut(s)	1641, 1650
Bpu10I	CCTNAGC	1 cut(s)	840
BpuEI	CTTGAG	1 cut(s)	167
BsaI	GGTCTC	2 cut(s)	839, 1100
BsaJI	CCNNGG	5 cut(s)	330, 478, 479, 632, 1222
BsaWI	WCCGGW	2 cut(s)	792, 1409
BsaXI	ACNNNNNCTCC	8 cut(s)	8, 38, 519, 549, 1442, 1472, 1654, 1684
Bsc4I	CCNNNNNNNGG	9 cut(s)	65, 362, 377, 479, 633, 792, 855, 874, 1540
Bse1I	ACTGG	7 cut(s)	371, 382, 649, 966, 1258, 1347, 1379
Bse21I	CCTNAGG	1 cut(s)	60
Bse3DI	GCAATG	1 cut(s)	655
BseAI	TCCGGA	2 cut(s)	792, 1409
BseBI	CCWGG	5 cut(s)	38, 480, 787, 875, 1503
BseDI	CCNNGG	5 cut(s)	330, 478, 479, 632, 1222
BseLI	CCNNNNNNNGG	9 cut(s)	65, 362, 377, 479, 633, 792, 855, 874, 1540
BseMI	GCAATG	1 cut(s)	655
BseMII	CTCAG	3 cut(s)	51, 1095, 1493
BseNI	ACTGG	7 cut(s)	371, 382, 649, 966, 1258, 1347, 1379
BseRI	GAGGAG	6 cut(s)	29, 122, 166, 507, 893, 1662
BseSI	GKGCMC	1 cut(s)	280
BseXI	GCAGC	6 cut(s)	86, 430, 589, 1128, 1150, 1625
BseYI	CCCAGC	1 cut(s)	1424
Bsh1236I	CGCG	2 cut(s)	399, 752
BshFI	GGCC	3 cut(s)	438, 640, 890
BshNI	GGYRCC	2 cut(s)	962, 1772
BsiHKAI	GWGCWC	1 cut(s)	537
BsiHKCI	CYCGRG	1 cut(s)	1785
BsiSI	CCGG	2 cut(s)	793, 1410
BslFI	GGGAC	1 cut(s)	481
BslI	CCNNNNNNNGG	9 cut(s)	65, 362, 377, 479, 633, 792, 855, 874, 1540
BsmAI	GTCTC	2 cut(s)	839, 1100
BsmFI	GGGAC	1 cut(s)	481
BsmI	GAATGC	1 cut(s)	177
BsnI	GGCC	3 cut(s)	438, 640, 890
Bso31I	GGTCTC	2 cut(s)	839, 1100
BsoBI	CYCGRG	1 cut(s)	1785
Bsp1286I	GDGCHC	2 cut(s)	280, 537
Bsp13I	TCCGGA	2 cut(s)	792, 1409
Bsp1407I	TGTACA	1 cut(s)	914
Bsp143I	GATC	8 cut(s)	6, 685, 718, 789, 1498, 1579, 1705, 1822
Bsp68I	TCGCGA	1 cut(s)	399
BspACI	CCGC	2 cut(s)	184, 752
BspANI	GGCC	3 cut(s)	438, 640, 890
BspCNI	CTCAG	3 cut(s)	52, 1096, 1492
BspEI	TCCGGA	2 cut(s)	792, 1409
BspFNI	CGCG	2 cut(s)	399, 752
BspHI	TCATGA	2 cut(s)	715, 1210
BspLI	GGNNCC	7 cut(s)	693, 791, 964, 1339, 1500, 1600, 1774
BspMAI	CTGCAG	2 cut(s)	448, 1615
BspMI	ACCTGC	2 cut(s)	1193, 1429
BspOI	GCTAGC	1 cut(s)	863
BspPI	GGATC	7 cut(s)	693, 784, 797, 1493, 1506, 1700, 1817
BspQI	GCTCTTC	1 cut(s)	1143
BspT107I	GGYRCC	2 cut(s)	962, 1772
BspTNI	GGTCTC	2 cut(s)	839, 1100
BsrDI	GCAATG	1 cut(s)	655
BsrGI	TGTACA	1 cut(s)	914
BsrI	ACTGG	7 cut(s)	371, 382, 649, 966, 1258, 1347, 1379
BssECI	CCNNGG	5 cut(s)	330, 478, 479, 632, 1222
BssMI	GATC	8 cut(s)	6, 685, 718, 789, 1498, 1579, 1705, 1822
BssT1I	CCWWGG	3 cut(s)	330, 632, 1222
Bst2UI	CCWGG	5 cut(s)	38, 480, 787, 875, 1503
Bst4CI	ACNGT	3 cut(s)	103, 392, 1322
Bst6I	CTCTTC	5 cut(s)	257, 486, 993, 1008, 1143
BstAUI	TGTACA	1 cut(s)	914
BstC8I	GCNNGC	1 cut(s)	861
BstDEI	CTNAG	6 cut(s)	60, 453, 840, 1104, 1479, 1762
BstENI	CCTNNNNNAGG	2 cut(s)	63, 872
BstFNI	CGCG	2 cut(s)	399, 752
BstH2I	RGCGCY	1 cut(s)	1311
BstHHI	GCGC	2 cut(s)	681, 1310
BstKTI	GATC	8 cut(s)	9, 688, 721, 792, 1501, 1582, 1708, 1825
BstMAI	GTCTC	2 cut(s)	839, 1100
BstMBI	GATC	8 cut(s)	6, 685, 718, 789, 1498, 1579, 1705, 1822
BstMWI	GCNNNNNNNGC	6 cut(s)	181, 193, 224, 238, 1150, 1667
BstNI	CCWGG	5 cut(s)	38, 480, 787, 875, 1503
BstNSI	RCATGY	2 cut(s)	772, 1609
BstPAI	GACNNNNGTC	2 cut(s)	1122, 1797
BstSCI	CCNGG	5 cut(s)	36, 478, 785, 873, 1501
BstSFI	CTRYAG	3 cut(s)	444, 1611, 1795
BstSLI	GKGCMC	1 cut(s)	280
BstUI	CGCG	2 cut(s)	399, 752
BstV1I	GCAGC	6 cut(s)	86, 430, 589, 1128, 1150, 1625
BstV2I	GAAGAC	1 cut(s)	386
BstX2I	RGATCY	3 cut(s)	789, 1498, 1822
BstXI	CCANNNNNNTGG	1 cut(s)	636
BstYI	RGATCY	3 cut(s)	789, 1498, 1822
Bsu36I	CCTNAGG	1 cut(s)	60
BsuRI	GGCC	3 cut(s)	438, 640, 890
BtsI	GCAGTG	2 cut(s)	239, 580
BtsIMutI	CAGTG	8 cut(s)	108, 239, 378, 580, 642, 964, 973, 1386
BtuMI	TCGCGA	1 cut(s)	399
BveI	ACCTGC	2 cut(s)	1193, 1429
Cac8I	GCNNGC	1 cut(s)	861
CaiI	CAGNNNCTG	2 cut(s)	377, 1121
CciI	TCATGA	2 cut(s)	715, 1210
CfoI	GCGC	2 cut(s)	681, 1310
Cfr13I	GGNCC	7 cut(s)	34, 437, 639, 692, 889, 1338, 1598
CseI	GACGC	2 cut(s)	372, 1605
Csp6I	GTAC	3 cut(s)	506, 915, 963
CviAII	CATG	7 cut(s)	86, 500, 539, 716, 769, 1211, 1606
CviQI	GTAC	3 cut(s)	506, 915, 963
DdeI	CTNAG	6 cut(s)	60, 453, 840, 1104, 1479, 1762
DpnI	GATC	8 cut(s)	8, 687, 720, 791, 1500, 1581, 1707, 1824
DpnII	GATC	8 cut(s)	6, 685, 718, 789, 1498, 1579, 1705, 1822
DrdI	GACNNNNNNGTC	1 cut(s)	382
DriI	GACNNNNNGTC	1 cut(s)	832
DseDI	GACNNNNNNGTC	1 cut(s)	382
Eam1104I	CTCTTC	5 cut(s)	257, 486, 993, 1008, 1143
Eam1105I	GACNNNNNGTC	1 cut(s)	832
EarI	CTCTTC	5 cut(s)	257, 486, 993, 1008, 1143
Ecl136II	GAGCTC	1 cut(s)	535
Eco130I	CCWWGG	3 cut(s)	330, 632, 1222
Eco24I	GRGCYC	1 cut(s)	537
Eco31I	GGTCTC	2 cut(s)	839, 1100
Eco32I	GATATC	1 cut(s)	1446
Eco47I	GGWCC	4 cut(s)	34, 692, 1338, 1598
Eco47III	AGCGCT	1 cut(s)	1309
Eco53kI	GAGCTC	1 cut(s)	535
Eco57I	CTGAAG	3 cut(s)	200, 743, 1167
Eco81I	CCTNAGG	1 cut(s)	60
Eco88I	CYCGRG	1 cut(s)	1785
EcoICRI	GAGCTC	1 cut(s)	535
EcoNI	CCTNNNNNAGG	2 cut(s)	63, 872
EcoO109I	RGGNCCY	4 cut(s)	34, 692, 1338, 1598
EcoRII	CCWGG	5 cut(s)	36, 478, 785, 873, 1501
EcoRV	GATATC	1 cut(s)	1446
EcoT14I	CCWWGG	3 cut(s)	330, 632, 1222
EcoT22I	ATGCAT	1 cut(s)	1083
EcoT38I	GRGCYC	1 cut(s)	537
ErhI	CCWWGG	3 cut(s)	330, 632, 1222
FaeI	CATG	7 cut(s)	89, 503, 542, 719, 772, 1214, 1609
FaqI	GGGAC	1 cut(s)	481
FatI	CATG	7 cut(s)	85, 499, 538, 715, 768, 1210, 1605
FbaI	TGATCA	1 cut(s)	1579
Fnu4HI	GCNGC	8 cut(s)	75, 185, 444, 578, 753, 1139, 1142, 1614
FriOI	GRGCYC	1 cut(s)	537
Fsp4HI	GCNGC	8 cut(s)	75, 185, 444, 578, 753, 1139, 1142, 1614
FspBI	CTAG	4 cut(s)	228, 860, 945, 1223
FspI	TGCGCA	1 cut(s)	680
GlaI	GCGC	2 cut(s)	680, 1309
GluI	GCNGC	8 cut(s)	75, 185, 444, 578, 753, 1139, 1142, 1614
GsaI	CCCAGC	1 cut(s)	1428
GsuI	CTGGAG	2 cut(s)	1641, 1650
HaeII	RGCGCY	1 cut(s)	1311
HaeIII	GGCC	3 cut(s)	438, 640, 890
HapII	CCGG	2 cut(s)	793, 1410
HgaI	GACGC	2 cut(s)	372, 1605
HhaI	GCGC	2 cut(s)	681, 1310
Hin1II	CATG	7 cut(s)	89, 503, 542, 719, 772, 1214, 1609
Hin6I	GCGC	2 cut(s)	679, 1308
HinP1I	GCGC	2 cut(s)	679, 1308
HincII	GTYRAC	1 cut(s)	1263
HindII	GTYRAC	1 cut(s)	1263
HindIII	AAGCTT	1 cut(s)	797
HinfI	GANTC	8 cut(s)	288, 494, 664, 673, 1112, 1118, 1724, 1805
HpaII	CCGG	2 cut(s)	793, 1410
Hpy166II	GTNNAC	6 cut(s)	368, 557, 663, 917, 1263, 1384
Hpy188I	TCNGA	7 cut(s)	258, 388, 723, 1096, 1117, 1450, 1498
Hpy8I	GTNNAC	6 cut(s)	368, 557, 663, 917, 1263, 1384
Hpy99I	CGWCG	1 cut(s)	128
HpyAV	CCTTC	6 cut(s)	59, 206, 403, 610, 721, 730
HpyCH4III	ACNGT	3 cut(s)	103, 392, 1322
HpyCH4IV	ACGT	1 cut(s)	601
HpyCH4V	TGCA	9 cut(s)	446, 503, 577, 823, 953, 1081, 1244, 1613, 1713
HpyF10VI	GCNNNNNNNGC	6 cut(s)	181, 193, 224, 238, 1150, 1667
HpyF3I	CTNAG	6 cut(s)	60, 453, 840, 1104, 1479, 1762
HpySE526I	ACGT	1 cut(s)	601
Hsp92II	CATG	7 cut(s)	89, 503, 542, 719, 772, 1214, 1609
HspAI	GCGC	2 cut(s)	679, 1308
Kpn2I	TCCGGA	2 cut(s)	792, 1409
KpnI	GGTACC	1 cut(s)	966
Ksp22I	TGATCA	1 cut(s)	1579
Kzo9I	GATC	8 cut(s)	6, 685, 718, 789, 1498, 1579, 1705, 1822
LguI	GCTCTTC	1 cut(s)	1143
LmnI	GCTCC	4 cut(s)	215, 532, 540, 1675
Lsp1109I	GCAGC	6 cut(s)	86, 430, 589, 1128, 1150, 1625
LweI	GCATC	6 cut(s)	532, 564, 1068, 1090, 1546, 1800
MaeI	CTAG	4 cut(s)	228, 860, 945, 1223
MaeII	ACGT	1 cut(s)	601
MaeIII	GTNAC	8 cut(s)	119, 270, 373, 622, 771, 1178, 1322, 1617
MalI	GATC	8 cut(s)	8, 687, 720, 791, 1500, 1581, 1707, 1824
MboI	GATC	8 cut(s)	6, 685, 718, 789, 1498, 1579, 1705, 1822
MfeI	CAATTG	1 cut(s)	668
MflI	RGATCY	3 cut(s)	789, 1498, 1822
MhlI	GDGCHC	2 cut(s)	280, 537
MluCI	AATT	8 cut(s)	128, 668, 883, 1003, 1282, 1546, 1553, 1694
MlyI	GAGTC	4 cut(s)	503, 658, 1112, 1799
MmeI	TCCRAC	2 cut(s)	591, 1276
Mph1103I	ATGCAT	1 cut(s)	1083
MroI	TCCGGA	2 cut(s)	792, 1409
MseI	TTAA	8 cut(s)	138, 189, 305, 870, 1035, 1043, 1166, 1401
MslI	CAYNNNNRTG	1 cut(s)	634
MspA1I	CMGCKG	1 cut(s)	1141
MspI	CCGG	2 cut(s)	793, 1410
MspR9I	CCNGG	5 cut(s)	38, 480, 787, 875, 1503
MunI	CAATTG	1 cut(s)	668
Mva1269I	GAATGC	1 cut(s)	177
MvaI	CCWGG	5 cut(s)	38, 480, 787, 875, 1503
MvnI	CGCG	2 cut(s)	399, 752
MwoI	GCNNNNNNNGC	6 cut(s)	181, 193, 224, 238, 1150, 1667
NdeII	GATC	8 cut(s)	6, 685, 718, 789, 1498, 1579, 1705, 1822
NheI	GCTAGC	1 cut(s)	859
NlaIII	CATG	7 cut(s)	89, 503, 542, 719, 772, 1214, 1609
NlaIV	GGNNCC	7 cut(s)	693, 791, 964, 1339, 1500, 1600, 1774
NmuCI	GTSAC	6 cut(s)	119, 270, 373, 771, 1322, 1617
NruI	TCGCGA	1 cut(s)	399
NsbI	TGCGCA	1 cut(s)	680
NsiI	ATGCAT	1 cut(s)	1083
NspI	RCATGY	2 cut(s)	772, 1609
OliI	CACNNNNGTG	1 cut(s)	634
PagI	TCATGA	2 cut(s)	715, 1210
PaqCI	CACCTGC	1 cut(s)	1193
PasI	CCCWGGG	1 cut(s)	479
PciI	ACATGT	2 cut(s)	768, 1605
PciSI	GCTCTTC	1 cut(s)	1143
PctI	GAATGC	1 cut(s)	177
PfeI	GAWTC	4 cut(s)	288, 673, 1112, 1724
PflMI	CCANNNNNTGG	3 cut(s)	377, 479, 855
PfoI	TCCNGGA	1 cut(s)	1501
PkrI	GCNGC	8 cut(s)	76, 186, 445, 579, 754, 1140, 1143, 1615
PleI	GAGTC	4 cut(s)	502, 658, 1112, 1799
PpsI	GAGTC	4 cut(s)	502, 658, 1112, 1799
PpuMI	RGGWCCY	4 cut(s)	34, 692, 1338, 1598
PscI	ACATGT	2 cut(s)	768, 1605
PshAI	GACNNNNGTC	2 cut(s)	1122, 1797
Psp124BI	GAGCTC	1 cut(s)	537
Psp5II	RGGWCCY	4 cut(s)	34, 692, 1338, 1598
Psp6I	CCWGG	5 cut(s)	36, 478, 785, 873, 1501
PspFI	CCCAGC	1 cut(s)	1424
PspGI	CCWGG	5 cut(s)	36, 478, 785, 873, 1501
PspN4I	GGNNCC	7 cut(s)	693, 791, 964, 1339, 1500, 1600, 1774
PspPI	GGNCC	7 cut(s)	34, 437, 639, 692, 889, 1338, 1598
PspPPI	RGGWCCY	4 cut(s)	34, 692, 1338, 1598
PstI	CTGCAG	2 cut(s)	448, 1615
PstNI	CAGNNNCTG	2 cut(s)	377, 1121
PsuI	RGATCY	3 cut(s)	789, 1498, 1822
PvuII	CAGCTG	1 cut(s)	1141
RruI	TCGCGA	1 cut(s)	399
RsaI	GTAC	3 cut(s)	507, 916, 964
RsaNI	GTAC	3 cut(s)	506, 915, 963
RseI	CAYNNNNRTG	1 cut(s)	634
SacI	GAGCTC	1 cut(s)	537
SapI	GCTCTTC	1 cut(s)	1143
SaqAI	TTAA	8 cut(s)	138, 189, 305, 870, 1035, 1043, 1166, 1401
SatI	GCNGC	8 cut(s)	75, 185, 444, 578, 753, 1139, 1142, 1614
Sau3AI	GATC	8 cut(s)	6, 685, 718, 789, 1498, 1579, 1705, 1822
Sau96I	GGNCC	7 cut(s)	34, 437, 639, 692, 889, 1338, 1598
SchI	GAGTC	4 cut(s)	503, 658, 1112, 1799
ScrFI	CCNGG	5 cut(s)	38, 480, 787, 875, 1503
SduI	GDGCHC	2 cut(s)	280, 537
SfaNI	GCATC	6 cut(s)	532, 564, 1068, 1090, 1546, 1800
SfcI	CTRYAG	3 cut(s)	444, 1611, 1795
SinI	GGWCC	4 cut(s)	34, 692, 1338, 1598
SmiMI	CAYNNNNRTG	1 cut(s)	634
SmlI	CTYRAG	1 cut(s)	146
SmoI	CTYRAG	1 cut(s)	146
Sse9I	AATT	8 cut(s)	128, 668, 883, 1003, 1282, 1546, 1553, 1694
SsiI	CCGC	2 cut(s)	184, 752
SspI	AATATT	2 cut(s)	818, 1048
SspMI	CTAG	4 cut(s)	228, 860, 945, 1223
SstI	GAGCTC	1 cut(s)	537
StyD4I	CCNGG	5 cut(s)	36, 478, 785, 873, 1501
StyI	CCWWGG	3 cut(s)	330, 632, 1222
TaaI	ACNGT	3 cut(s)	103, 392, 1322
TaiI	ACGT	1 cut(s)	604
TaqI	TCGA	2 cut(s)	1512, 1808
TaqII	GACCGA	2 cut(s)	798, 1124
TasI	AATT	8 cut(s)	128, 668, 883, 1003, 1282, 1546, 1553, 1694
TatI	WGTACW	1 cut(s)	914
TauI	GCSGC	2 cut(s)	187, 755
TfiI	GAWTC	4 cut(s)	288, 673, 1112, 1724
Tru1I	TTAA	8 cut(s)	138, 189, 305, 870, 1035, 1043, 1166, 1401
Tru9I	TTAA	8 cut(s)	138, 189, 305, 870, 1035, 1043, 1166, 1401
TscAI	CASTG	8 cut(s)	108, 239, 378, 587, 649, 964, 973, 1386
TseFI	GTSAC	6 cut(s)	119, 270, 373, 771, 1322, 1617
TseI	GCWGC	6 cut(s)	74, 443, 577, 1138, 1141, 1613
Tsp45I	GTSAC	6 cut(s)	119, 270, 373, 771, 1322, 1617
TspDTI	ATGAA	6 cut(s)	16, 102, 504, 794, 1011, 1023
TspGWI	ACGGA	1 cut(s)	1698
TspRI	CASTG	8 cut(s)	108, 239, 378, 587, 649, 964, 973, 1386
Van91I	CCANNNNNTGG	3 cut(s)	377, 479, 855
VpaK11BI	GGWCC	4 cut(s)	34, 692, 1338, 1598
XagI	CCTNNNNNAGG	2 cut(s)	63, 872
XapI	RAATTY	2 cut(s)	883, 1694
XceI	RCATGY	2 cut(s)	772, 1609
XmaJI	CCTAGG	1 cut(s)	1222
XspI	CTAG	4 cut(s)	228, 860, 945, 1223
Zsp2I	ATGCAT	1 cut(s)	1083

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.