Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr07

Orthogroup ID

OG0001963

Physical Location & Seq

Genomic Coordinates Forward (+)

4951038 .. 4952809

Gene Structure 1772 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD07G1053500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1545 bp

ATGGAGATCTTTGAGGAATTGGGGTTAAGTGGTGATCTTGACTTTCTTTCGGCTCCCCTCGAGGAAGATGAGGCAGCCCCAGAGCATGACCCAGAGGCAACAGTGGAGGAGGATAGCAGCGATGAAGAATTGGGGGTTGATGAGCTTGAGAAGAGGGTGTGGAAATATAGAATGCAACTGAAGCGGCTTAAGGAACAAGATAAAGGAACAGAAGGAGCTGACAATGCAAGGCAGTGCCAACCAAAGGAACAATCTCAGAGGAAGAAGATGTCACGGGCACAAGATGCAATCCTGAAATATATGTTAAGAATGATGGAAGTTTGCAAAGCCGAGGGTTTTGTTTATGGGATTGTCCCCAAAAAAGGTAAACCAGTGACTGGTGCTTCTGACAATCTTCGTGAATGGTGGAAGGGAAAAGTAAGATTTGGTCGTAATGGCCCTGCTGCAATTTCTAAGTATCTGGCTGAACATTCAATCCCTGGGAAGAATGAAGACTCCAATGCAGTGGCATCAACTCATCACACCTTACAGGAGCTCCAAGATGGCACTCTTGGTTCACTTTTGTCGGCTTTGCTTCAGCACTGCAATCCACCCCAAAGACGTTTCCCCTTGGACAAGGGTGTTGCCCCACCTTGGTGGCCCACTGGCAATGAGGAATGGTGGACTCAATTGAATCTGCCCAAGGATCAGGGTCCTCCACCATACAAGAAGCCTCATGATCTGAAGAAGGCTTGGAAGGTGAGTGTTCTCACAGCTGTGATAAAGCACATGTCGCCCGATATTGCCAAGATCCAGAAGCTTGTTCATCAGTCTAGATGTTTGCAGGACAAAATGACTGCTAAGGAGAGCACTACTTGGCTCGCCATCATTAACCAGGAGGAAAATTTGGCCCAGATGTTATATCCTGATAGATTTCCACCTCCATTGCCTCTTTGGAGTGAGTCTTTCGCAATCAGTTGTACCAGTGATTATGATGTTGAAGTGGTTGATGACGAACAGAATAATGAAGTAGAGGATTGCCAACCTCTTTTTAATCACTTCAATACTGAAACCGCTGGACAAAGAGATGTACCGATGCATCATATTAAGGGAGAGCTGGTTGAGACCAATTCGAACTTTGGTCAAAAGAGGAAGCAGCTGCCTGAAGAGCCACACATGATTCTAAATCATCAGATTTACACCTGCGAATATCTGCATTGCCCGTATCATGATTATCGCTTAGGCTTTCTCGACATAACTGCAAGAAATAATCACCAGTTGACTTGTTCACACAGGAATAATTCTTCACAAGTGTTTGGAATGTCAAACTTTAATTGTAACAGTGACAAACTTGGGGGCTCTCTACCCGCTTCCCAACCTATTCCAGCTATTCAACAACAAGTGAACCAGACAAGTTGTTTGAATGCTTCGGGACATGGAGTTGCTGGGGATGGGCAGAAAATGATATATGAGCATGTGTCATTCTATGATAGTAATATTCAGCAGAGCAAGAACTCGGATCCTGGAAGTCTCAATGGGGGTGGTTATGGGACCCAACATGTCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000160 GO:0000976 GO:0001067 GO:0001666 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0006950 GO:0006952 GO:0007154 GO:0007165 GO:0008150 GO:0009605 GO:0009607 GO:0009617 GO:0009628 GO:0009719 GO:0009723 GO:0009725 GO:0009743 GO:0009755 GO:0009756 GO:0009873 GO:0009889 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010039 GO:0010182 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0023052 GO:0031323 GO:0031326 GO:0032870 GO:0035556 GO:0036293 GO:0042221 GO:0042742 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044212 GO:0044424 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051252 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0065007 GO:0070482 GO:0070887 GO:0071241 GO:0071248 GO:0071281 GO:0071310 GO:0071322 GO:0071369 GO:0071495 GO:0080090 GO:0097159 GO:0098542 GO:0140110 GO:1901363 GO:1901700 GO:1901701 GO:1903506 GO:1990837 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

ko04016 ko04075 map04016 map04075

Pfam Domains

Protein Families

EIN3

Protein Analysis

515

Amino Acids

58.03

Weight (kDa)

5.63

Isoelectric Point (pI)

49.03

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
EIN3_DNA-bd	PF04873	47 - 294	2.7e-121	Ethylene insensitive 3, DNA-binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001963)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G27050 AT3G20770
fragaria_vesca	FvH4_1g28720 FvH4_1g28720 FvH4_7g04940 FvH4_7g04940 FvH4_7g04940 FvH4_7g04940 FvH4_7g04940
malus_domestica	MD02G1266200.v1.1 MD02G1266300.v1.1 MD07G1053500.v1.1 MD07G1053800.v1.1 MD08G1245800.v1.1 MD15G1441000.v1.1
prunus_persica	Prupe.2G058400_v2.0.a1 Prupe.2G058500_v2.0.a1 Prupe.2G070300_v2.0.a1
pyrus_communis	pycom02g22750 pycom02g22760 pycom07g04140 pycom07g04160 pycom08g21330 pycom15g38880
rosa_chinensis	RchiOBHm_Chr1g0331011 RchiOBHm_Chr5g0049961
rosa_laevigata	RLG00000029759 RLG00000034672
rosa_multiflora	Rmu_sc0000333.1_g000038 Rmu_sc0013622.1_g000002 Rmu_sc0013622.1_g000003
rosa_roxburghii	Rroxscaffold_1G00031290 Rroxscaffold_4G00319550
rosa_rugosa	Rorug01G0091600 Rorug05G0255200
rosa_samantha	Rh1AG113300 Rh1BG087200 Rh1CG110200 Rh1DG118900 Rh5AG329100 Rh5BG339600 Rh5CG365600 Rh5DG352200
rosa_wichuraiana	Rw1G009240 Rw5G031070

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1190
AbsI	CCTCGAGG	1 cut(s)	59
Acc36I	ACCTGC	1 cut(s)	1190
AccB7I	CCANNNNNTGG	1 cut(s)	377
AciI	CCGC	3 cut(s)	184, 1053, 1347
AclWI	GGATC	4 cut(s)	693, 784, 1493, 1506
AcsI	RAATTY	1 cut(s)	883
AcuI	CTGAAG	4 cut(s)	200, 560, 743, 1164
AfaI	GTAC	2 cut(s)	961, 1071
AfiI	CCNNNNNNNGG	4 cut(s)	244, 362, 377, 633
AflII	CTTAAG	1 cut(s)	188
AflIII	ACRYGT	2 cut(s)	768, 1537
AgsI	TTSAA	6 cut(s)	474, 673, 980, 1042, 1373, 1402
AjnI	CCWGG	3 cut(s)	478, 873, 1501
AleI	CACNNNNGTG	2 cut(s)	634, 755
AloI	GAACNNNNNNTCC	2 cut(s)	459, 491
AluBI	AGCT	8 cut(s)	145, 218, 535, 755, 799, 1096, 1138, 1367
AluI	AGCT	8 cut(s)	145, 218, 535, 755, 799, 1096, 1138, 1367
Alw21I	GWGCWC	2 cut(s)	537, 851
Alw26I	GTCTC	2 cut(s)	1097, 1514
AlwI	GGATC	4 cut(s)	693, 784, 1493, 1506
AlwNI	CAGNNNCTG	1 cut(s)	377
Ama87I	CYCGRG	1 cut(s)	59
AoxI	GGCC	3 cut(s)	436, 638, 888
ApeKI	GCWGC	5 cut(s)	74, 117, 443, 1135, 1138
ApoI	RAATTY	1 cut(s)	883
ArsI	GACNNNNNNTTYG	2 cut(s)	1381, 1413
AspS9I	GGNCC	5 cut(s)	437, 639, 692, 889, 1530
AsuHPI	GGTGA	3 cut(s)	44, 751, 1244
AsuII	TTCGAA	1 cut(s)	1112
AvaI	CYCGRG	1 cut(s)	59
AvaII	GGWCC	2 cut(s)	692, 1530
BaeGI	GKGCMC	1 cut(s)	280
BamHI	GGATCC	1 cut(s)	1498
BanII	GRGCYC	2 cut(s)	537, 1340
BbsI	GAAGAC	1 cut(s)	498
Bbv12I	GWGCWC	2 cut(s)	537, 851
BbvI	GCAGC	5 cut(s)	86, 129, 430, 1125, 1147
BccI	CCATC	4 cut(s)	307, 536, 872, 1424
BcgI	CGANNNNNNTGC	2 cut(s)	1220, 1254
BciT130I	CCWGG	3 cut(s)	480, 875, 1503
BcoDI	GTCTC	2 cut(s)	1097, 1514
BfaI	CTAG	1 cut(s)	813
BfrI	CTTAAG	1 cut(s)	188
BfuAI	ACCTGC	1 cut(s)	1190
BglII	AGATCT	1 cut(s)	6
BisI	GCNGC	6 cut(s)	75, 118, 185, 444, 1136, 1139
BlsI	GCNGC	6 cut(s)	76, 119, 186, 445, 1137, 1140
Bme1390I	CCNGG	3 cut(s)	480, 875, 1503
Bme18I	GGWCC	2 cut(s)	692, 1530
BmeT110I	CYCGRG	1 cut(s)	59
BmgT120I	GGNCC	5 cut(s)	437, 639, 692, 889, 1530
BmiI	GGNNCC	5 cut(s)	54, 693, 1500, 1531, 1532
BmrFI	CCNGG	3 cut(s)	480, 875, 1503
BmsI	GCATC	4 cut(s)	274, 518, 1065, 1087
BpiI	GAAGAC	1 cut(s)	498
Bpu10I	CCTNAGC	2 cut(s)	840, 1219
Bpu14I	TTCGAA	1 cut(s)	1112
BpuEI	CTTGAG	1 cut(s)	167
BsaI	GGTCTC	1 cut(s)	1097
BsaJI	CCNNGG	6 cut(s)	330, 478, 479, 609, 632, 681
BsaXI	ACNNNNNCTCC	4 cut(s)	519, 549, 1083, 1113
Bsc4I	CCNNNNNNNGG	4 cut(s)	244, 362, 377, 633
Bse1I	ACTGG	5 cut(s)	371, 382, 649, 963, 1255
Bse3DI	GCAATG	3 cut(s)	655, 923, 1195
BseBI	CCWGG	3 cut(s)	480, 875, 1503
BseDI	CCNNGG	6 cut(s)	330, 478, 479, 609, 632, 681
BseGI	GGATG	1 cut(s)	1435
BseLI	CCNNNNNNNGG	4 cut(s)	244, 362, 377, 633
BseMI	GCAATG	3 cut(s)	655, 923, 1195
BseMII	CTCAG	1 cut(s)	269
BseNI	ACTGG	5 cut(s)	371, 382, 649, 963, 1255
BseRI	GAGGAG	1 cut(s)	122
BseSI	GKGCMC	1 cut(s)	280
BseXI	GCAGC	5 cut(s)	86, 129, 430, 1125, 1147
BseYI	CCCAGC	1 cut(s)	1424
BshFI	GGCC	3 cut(s)	438, 640, 890
BsiHKAI	GWGCWC	2 cut(s)	537, 851
BsiHKCI	CYCGRG	1 cut(s)	59
BslFI	GGGAC	2 cut(s)	338, 1425
BslI	CCNNNNNNNGG	4 cut(s)	244, 362, 377, 633
BsmAI	GTCTC	2 cut(s)	1097, 1514
BsmFI	GGGAC	2 cut(s)	338, 1425
BsmI	GAATGC	2 cut(s)	177, 1408
BsnI	GGCC	3 cut(s)	438, 640, 890
Bso31I	GGTCTC	1 cut(s)	1097
BsoBI	CYCGRG	1 cut(s)	59
Bsp119I	TTCGAA	1 cut(s)	1112
Bsp1286I	GDGCHC	4 cut(s)	280, 537, 851, 1340
Bsp143I	GATC	6 cut(s)	6, 34, 685, 718, 789, 1498
BspACI	CCGC	3 cut(s)	184, 1053, 1347
BspANI	GGCC	3 cut(s)	438, 640, 890
BspCNI	CTCAG	1 cut(s)	268
BspHI	TCATGA	2 cut(s)	715, 1207
BspLI	GGNNCC	5 cut(s)	54, 693, 1500, 1531, 1532
BspMI	ACCTGC	1 cut(s)	1190
BspPI	GGATC	4 cut(s)	693, 784, 1493, 1506
BspQI	GCTCTTC	1 cut(s)	1140
BspT104I	TTCGAA	1 cut(s)	1112
BspTI	CTTAAG	1 cut(s)	188
BspTNI	GGTCTC	1 cut(s)	1097
BsrDI	GCAATG	3 cut(s)	655, 923, 1195
BsrI	ACTGG	5 cut(s)	371, 382, 649, 963, 1255
BssECI	CCNNGG	6 cut(s)	330, 478, 479, 609, 632, 681
BssMI	GATC	6 cut(s)	6, 34, 685, 718, 789, 1498
BssT1I	CCWWGG	3 cut(s)	609, 632, 681
Bst2UI	CCWGG	3 cut(s)	480, 875, 1503
Bst4CI	ACNGT	2 cut(s)	103, 1322
Bst6I	CTCTTC	2 cut(s)	146, 1140
BstAFI	CTTAAG	1 cut(s)	188
BstAPI	GCANNNNNTGC	1 cut(s)	284
BstBI	TTCGAA	1 cut(s)	1112
BstC8I	GCNNGC	1 cut(s)	861
BstDEI	CTNAG	4 cut(s)	255, 453, 840, 1219
BstF5I	GGATG	1 cut(s)	1435
BstKTI	GATC	6 cut(s)	9, 37, 688, 721, 792, 1501
BstMAI	GTCTC	2 cut(s)	1097, 1514
BstMBI	GATC	6 cut(s)	6, 34, 685, 718, 789, 1498
BstMWI	GCNNNNNNNGC	5 cut(s)	181, 224, 284, 772, 1147
BstNI	CCWGG	3 cut(s)	480, 875, 1503
BstNSI	RCATGY	3 cut(s)	772, 1457, 1541
BstSCI	CCNGG	3 cut(s)	478, 873, 1501
BstSLI	GKGCMC	1 cut(s)	280
BstV1I	GCAGC	5 cut(s)	86, 129, 430, 1125, 1147
BstV2I	GAAGAC	1 cut(s)	498
BstX2I	RGATCY	3 cut(s)	6, 789, 1498
BstXI	CCANNNNNNTGG	2 cut(s)	505, 636
BstYI	RGATCY	3 cut(s)	6, 789, 1498
BsuRI	GGCC	3 cut(s)	438, 640, 890
BtgZI	GCGATG	1 cut(s)	135
BtsCI	GGATG	1 cut(s)	1435
BtsI	GCAGTG	3 cut(s)	239, 510, 580
BtsIMutI	CAGTG	8 cut(s)	108, 239, 378, 510, 580, 642, 970, 1327
BveI	ACCTGC	1 cut(s)	1190
Cac8I	GCNNGC	1 cut(s)	861
CaiI	CAGNNNCTG	1 cut(s)	377
CciI	TCATGA	2 cut(s)	715, 1207
Cfr13I	GGNCC	5 cut(s)	437, 639, 692, 889, 1530
Csp6I	GTAC	2 cut(s)	960, 1070
CspCI	CAANNNNNGTGG	4 cut(s)	906, 941, 1501, 1536
CviAII	CATG	8 cut(s)	86, 716, 769, 1156, 1208, 1415, 1454, 1538
CviQI	GTAC	2 cut(s)	960, 1070
DdeI	CTNAG	4 cut(s)	255, 453, 840, 1219
DpnI	GATC	6 cut(s)	8, 36, 687, 720, 791, 1500
DpnII	GATC	6 cut(s)	6, 34, 685, 718, 789, 1498
Eam1104I	CTCTTC	2 cut(s)	146, 1140
EarI	CTCTTC	2 cut(s)	146, 1140
Ecl136II	GAGCTC	1 cut(s)	535
Eco130I	CCWWGG	3 cut(s)	609, 632, 681
Eco24I	GRGCYC	2 cut(s)	537, 1340
Eco31I	GGTCTC	1 cut(s)	1097
Eco47I	GGWCC	2 cut(s)	692, 1530
Eco53kI	GAGCTC	1 cut(s)	535
Eco57I	CTGAAG	4 cut(s)	200, 560, 743, 1164
Eco88I	CYCGRG	1 cut(s)	59
EcoICRI	GAGCTC	1 cut(s)	535
EcoO109I	RGGNCCY	2 cut(s)	692, 1530
EcoRII	CCWGG	3 cut(s)	478, 873, 1501
EcoT14I	CCWWGG	3 cut(s)	609, 632, 681
EcoT22I	ATGCAT	1 cut(s)	1080
EcoT38I	GRGCYC	2 cut(s)	537, 1340
ErhI	CCWWGG	3 cut(s)	609, 632, 681
FaeI	CATG	8 cut(s)	89, 719, 772, 1159, 1211, 1418, 1457, 1541
FaqI	GGGAC	2 cut(s)	338, 1425
FatI	CATG	8 cut(s)	85, 715, 768, 1155, 1207, 1414, 1453, 1537
FauI	CCCGC	1 cut(s)	1354
Fnu4HI	GCNGC	6 cut(s)	75, 118, 185, 444, 1136, 1139
FokI	GGATG	1 cut(s)	1442
FriOI	GRGCYC	2 cut(s)	537, 1340
Fsp4HI	GCNGC	6 cut(s)	75, 118, 185, 444, 1136, 1139
FspBI	CTAG	1 cut(s)	813
GluI	GCNGC	6 cut(s)	75, 118, 185, 444, 1136, 1139
GsaI	CCCAGC	1 cut(s)	1428
HaeIII	GGCC	3 cut(s)	438, 640, 890
Hin1II	CATG	8 cut(s)	89, 719, 772, 1159, 1211, 1418, 1457, 1541
HincII	GTYRAC	1 cut(s)	1260
HindII	GTYRAC	1 cut(s)	1260
HindIII	AAGCTT	1 cut(s)	797
HinfI	GANTC	5 cut(s)	494, 664, 673, 941, 1159
HphI	GGTGA	3 cut(s)	44, 751, 1244
Hpy166II	GTNNAC	6 cut(s)	368, 557, 663, 1260, 1268, 1384
Hpy188I	TCNGA	6 cut(s)	258, 388, 723, 1173, 1498, 1544
Hpy8I	GTNNAC	6 cut(s)	368, 557, 663, 1260, 1268, 1384
HpyAV	CCTTC	4 cut(s)	206, 403, 721, 730
HpyCH4III	ACNGT	2 cut(s)	103, 1322
HpyCH4IV	ACGT	1 cut(s)	601
HpyF10VI	GCNNNNNNNGC	5 cut(s)	181, 224, 284, 772, 1147
HpyF3I	CTNAG	4 cut(s)	255, 453, 840, 1219
HpySE526I	ACGT	1 cut(s)	601
Hsp92II	CATG	8 cut(s)	89, 719, 772, 1159, 1211, 1418, 1457, 1541
KflI	GGGWCCC	1 cut(s)	1530
Kzo9I	GATC	6 cut(s)	6, 34, 685, 718, 789, 1498
LguI	GCTCTTC	1 cut(s)	1140
LmnI	GCTCC	4 cut(s)	58, 215, 532, 540
Lsp1109I	GCAGC	5 cut(s)	86, 129, 430, 1125, 1147
LweI	GCATC	4 cut(s)	274, 518, 1065, 1087
MaeI	CTAG	1 cut(s)	813
MaeII	ACGT	1 cut(s)	601
MaeIII	GTNAC	4 cut(s)	270, 373, 1316, 1322
MalI	GATC	6 cut(s)	8, 36, 687, 720, 791, 1500
MboI	GATC	6 cut(s)	6, 34, 685, 718, 789, 1498
MfeI	CAATTG	1 cut(s)	668
MflI	RGATCY	3 cut(s)	6, 789, 1498
MhlI	GDGCHC	4 cut(s)	280, 537, 851, 1340
MluCI	AATT	8 cut(s)	17, 128, 447, 668, 883, 1108, 1279, 1312
MlyI	GAGTC	3 cut(s)	488, 658, 950
Mph1103I	ATGCAT	1 cut(s)	1080
MseI	TTAA	7 cut(s)	26, 189, 305, 870, 1032, 1086, 1311
MslI	CAYNNNNRTG	2 cut(s)	634, 755
MspA1I	CMGCKG	3 cut(s)	755, 1055, 1138
MspCI	CTTAAG	1 cut(s)	188
MspR9I	CCNGG	3 cut(s)	480, 875, 1503
MunI	CAATTG	1 cut(s)	668
Mva1269I	GAATGC	2 cut(s)	177, 1408
MvaI	CCWGG	3 cut(s)	480, 875, 1503
MwoI	GCNNNNNNNGC	5 cut(s)	181, 224, 284, 772, 1147
NdeII	GATC	6 cut(s)	6, 34, 685, 718, 789, 1498
NlaIII	CATG	8 cut(s)	89, 719, 772, 1159, 1211, 1418, 1457, 1541
NlaIV	GGNNCC	5 cut(s)	54, 693, 1500, 1531, 1532
NmeAIII	GCCGAG	1 cut(s)	355
NmuCI	GTSAC	3 cut(s)	270, 373, 1322
NsiI	ATGCAT	1 cut(s)	1080
NspI	RCATGY	3 cut(s)	772, 1457, 1541
NspV	TTCGAA	1 cut(s)	1112
OliI	CACNNNNGTG	2 cut(s)	634, 755
PaeR7I	CTCGAG	1 cut(s)	59
PagI	TCATGA	2 cut(s)	715, 1207
PaqCI	CACCTGC	1 cut(s)	1190
PasI	CCCWGGG	1 cut(s)	479
PciI	ACATGT	2 cut(s)	768, 1537
PciSI	GCTCTTC	1 cut(s)	1140
PctI	GAATGC	2 cut(s)	177, 1408
PfeI	GAWTC	2 cut(s)	673, 1159
PflMI	CCANNNNNTGG	1 cut(s)	377
PfoI	TCCNGGA	1 cut(s)	1501
PkrI	GCNGC	6 cut(s)	76, 119, 186, 445, 1137, 1140
PleI	GAGTC	3 cut(s)	488, 658, 949
PpsI	GAGTC	3 cut(s)	488, 658, 949
PpuMI	RGGWCCY	2 cut(s)	692, 1530
PscI	ACATGT	2 cut(s)	768, 1537
Psp124BI	GAGCTC	1 cut(s)	537
Psp5II	RGGWCCY	2 cut(s)	692, 1530
Psp6I	CCWGG	3 cut(s)	478, 873, 1501
PspFI	CCCAGC	1 cut(s)	1424
PspGI	CCWGG	3 cut(s)	478, 873, 1501
PspN4I	GGNNCC	5 cut(s)	54, 693, 1500, 1531, 1532
PspPI	GGNCC	5 cut(s)	437, 639, 692, 889, 1530
PspPPI	RGGWCCY	2 cut(s)	692, 1530
PspXI	VCTCGAGB	1 cut(s)	59
PstNI	CAGNNNCTG	1 cut(s)	377
PsuI	RGATCY	3 cut(s)	6, 789, 1498
PvuII	CAGCTG	2 cut(s)	755, 1138
RsaI	GTAC	2 cut(s)	961, 1071
RsaNI	GTAC	2 cut(s)	960, 1070
RseI	CAYNNNNRTG	2 cut(s)	634, 755
SacI	GAGCTC	1 cut(s)	537
SapI	GCTCTTC	1 cut(s)	1140
SaqAI	TTAA	7 cut(s)	26, 189, 305, 870, 1032, 1086, 1311
SatI	GCNGC	6 cut(s)	75, 118, 185, 444, 1136, 1139
Sau3AI	GATC	6 cut(s)	6, 34, 685, 718, 789, 1498
Sau96I	GGNCC	5 cut(s)	437, 639, 692, 889, 1530
SchI	GAGTC	3 cut(s)	488, 658, 950
ScrFI	CCNGG	3 cut(s)	480, 875, 1503
SduI	GDGCHC	4 cut(s)	280, 537, 851, 1340
SfaNI	GCATC	4 cut(s)	274, 518, 1065, 1087
Sfr274I	CTCGAG	1 cut(s)	59
SfuI	TTCGAA	1 cut(s)	1112
SinI	GGWCC	2 cut(s)	692, 1530
SlaI	CTCGAG	1 cut(s)	59
SmiMI	CAYNNNNRTG	2 cut(s)	634, 755
SmlI	CTYRAG	3 cut(s)	59, 146, 188
SmoI	CTYRAG	3 cut(s)	59, 146, 188
Sse9I	AATT	8 cut(s)	17, 128, 447, 668, 883, 1108, 1279, 1312
SsiI	CCGC	3 cut(s)	184, 1053, 1347
SspI	AATATT	1 cut(s)	1477
SspMI	CTAG	1 cut(s)	813
SstI	GAGCTC	1 cut(s)	537
StyD4I	CCNGG	3 cut(s)	478, 873, 1501
StyI	CCWWGG	3 cut(s)	609, 632, 681
TaaI	ACNGT	2 cut(s)	103, 1322
TaiI	ACGT	1 cut(s)	604
TaqI	TCGA	3 cut(s)	60, 1112, 1230
TasI	AATT	8 cut(s)	17, 128, 447, 668, 883, 1108, 1279, 1312
TauI	GCSGC	1 cut(s)	187
TfiI	GAWTC	2 cut(s)	673, 1159
Tru1I	TTAA	7 cut(s)	26, 189, 305, 870, 1032, 1086, 1311
Tru9I	TTAA	7 cut(s)	26, 189, 305, 870, 1032, 1086, 1311
TscAI	CASTG	8 cut(s)	108, 239, 378, 510, 587, 649, 970, 1327
TseFI	GTSAC	3 cut(s)	270, 373, 1322
TseI	GCWGC	5 cut(s)	74, 117, 443, 1135, 1138
Tsp45I	GTSAC	3 cut(s)	270, 373, 1322
TspDTI	ATGAA	4 cut(s)	138, 504, 794, 1020
TspRI	CASTG	8 cut(s)	108, 239, 378, 510, 587, 649, 970, 1327
Van91I	CCANNNNNTGG	1 cut(s)	377
Vha464I	CTTAAG	1 cut(s)	188
VpaK11BI	GGWCC	2 cut(s)	692, 1530
XapI	RAATTY	1 cut(s)	883
XbaI	TCTAGA	1 cut(s)	812
XceI	RCATGY	3 cut(s)	772, 1457, 1541
XcmI	CCANNNNNNNNNTGG	1 cut(s)	930
XhoI	CTCGAG	1 cut(s)	59
XspI	CTAG	1 cut(s)	813
Zsp2I	ATGCAT	1 cut(s)	1080

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.