Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr7

Orthogroup ID

OG0001963

Physical Location & Seq

Genomic Coordinates Forward (+)

3605224 .. 3607056

Gene Structure 1833 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom07g04160.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1833 bp

ATGGGGATCTTTGAGGAAATGGGTTTCTGTGGTAATCTTGACTTTCTTACGGCGCCATCTGGGGAAGGTGATGCAGCTCCAGAGCATGAACCAGAGGCAGCAGTGGAGGAGGATTATAGTGATGAAGAAATGGATGTTGGTGAGCTTGAGAGGAGGATGTGGAGGGATCGAATGCTATTGAAGAGGTTGAGAGAACAAACTAAGGGAAAGGAAAGGGTTGATAATGCAAGACAGCGTCAGTCACAGGAACAAGCTCGGAGGAAGAAGATGTCACGGGCACAAGATGGAATCCTGAAATATATGCTGAAAATGATGGAAGTTTGCAAAGCTCAGGGTTTTGTTTATGGAATTATCCCTGAAAAAGGAAAACCAGTGAGTGGCGCCTCTGACAATCTGCGAGCATGGTGGAAGGAAAAAGTAAGATTTGATCGTAATGGTCCGGCTGCAATTTCTAAGTATCAGGCCGATCATTCAATCCCTGGGAAGAATGAAGACTGCAGTGCAGTGGTATCTACTCCTCACACTCTACAGGAGTTGCAAGACACCACTCTTGGTTCACTTTTGTCGGCTTTGATGCAGCACTGCGATCCACCCCAAAGACGTTTCCCCTTGGAGAAAGGTTTTGCCCCACCTTGGTGGCCTACTGGTAATGAGGAATGGTGGCCTCAATTAAATGTGCCCAAGGATCAGGGTCCTCCCCCATACAAGAAGCCTCATGATCTGAAGAAGGCTTGGAAGGTGAGTGTTCTTACGGCTGTGATAAAGCACATGTCACCCGATATTGCCAAGATCCGCAAGCTTGTTCGTCAGTCCAAGTGTTTGCAAGACAAAATGACTGCTAAGGAGAGCGCCACTTGGCTAGCCATTATACACCAGGAGGAAGCTTTGGCGCGAAGGTTATATCCTGATAGATGTCCACCTCCACCGGCTGGTGGTGGCGGGTCTCTTGCAATCAGTGGTACCAGTGATTATGACGTTGAGTGTGTTGATGATGACGAAAATGTTGAAATAGAGGATTGCAAACCTCTTGTTAATCACTTCAACATTGGAAGTGCTGGTCAAAGAGAGAGGCTGGTACCTCAGATTAAGGGAGAGCTGATTGAGATCAACTCGGATTTTGGTCAAAAGAGGAAGCAGTTGTCTGAAGAGCCACAAATGATGTTAAATCAGAATATTTTCACCTGCGAATATCTGCAGTGCCCGTATCATGATTATCACCTAGGCTTTCTCGATATAACTGCAAGAAACAATCACCAGTTGAATTGTCCATTCCAGAGTAATTCTACACAAGTGTTTGGAATGTCAAGCGGAATGTCTGGCTTTCAGCTTCACAATGAAAAACCTGTGGGATTCTCTCTACCCATTGCTCAACCACCTGCACCAGCAAGTCAACCACCAGTGAACCAGGCAAGTAGGTTTAATGCTTCGGGACTTGGACTTGTCGATAATGGTCAGAAATCTGAGCTTATGTCATTTTATGACAGTAATATTCAGCAGAATAAGAACTGCAATCCTGCAAACCTCCATATCGTAGATAACCGCAACTCGCAGCAGTCAAAATATCAGTTTCCAATGAATGACAACTTTTTTGGTCAAGGGGTGGATGTAGGACGCAACATCAACATGTCTGAACTGGCGCCTATGCCTATGCTTCATCCAGGTTTTGCATCCCCGGAAGTTCAGTTTGATCAGTGCATGGCATTTGATCCTCCATTTGGTAACAATACCAATGAAAATGTTGACATAAGATTTGAGTCCCCCTTGCACTTGGCACCGGCTGATTATAATGTTATGGACCAACCGCTGAAGCAAGATGCATCCGTTTGGTTCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000160 GO:0000976 GO:0001067 GO:0001666 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0006950 GO:0006952 GO:0007154 GO:0007165 GO:0008150 GO:0009605 GO:0009607 GO:0009617 GO:0009628 GO:0009719 GO:0009723 GO:0009725 GO:0009743 GO:0009755 GO:0009756 GO:0009873 GO:0009889 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010039 GO:0010182 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0023052 GO:0031323 GO:0031326 GO:0032870 GO:0035556 GO:0036293 GO:0042221 GO:0042742 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044212 GO:0044424 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051252 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0065007 GO:0070482 GO:0070887 GO:0071241 GO:0071248 GO:0071281 GO:0071310 GO:0071322 GO:0071369 GO:0071495 GO:0080090 GO:0097159 GO:0098542 GO:0140110 GO:1901363 GO:1901700 GO:1901701 GO:1903506 GO:1990837 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

ko04016 ko04075 map04016 map04075

Pfam Domains

Protein Families

EIN3

Protein Analysis

611

Amino Acids

68.79

Weight (kDa)

5.56

Isoelectric Point (pI)

59.11

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
EIN3_DNA-bd	PF04873	48 - 294	2.5e-131	Ethylene insensitive 3, DNA-binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001963)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G27050 AT3G20770
fragaria_vesca	FvH4_1g28720 FvH4_1g28720 FvH4_7g04940 FvH4_7g04940 FvH4_7g04940 FvH4_7g04940 FvH4_7g04940
malus_domestica	MD02G1266200.v1.1 MD02G1266300.v1.1 MD07G1053500.v1.1 MD07G1053800.v1.1 MD08G1245800.v1.1 MD15G1441000.v1.1
prunus_persica	Prupe.2G058400_v2.0.a1 Prupe.2G058500_v2.0.a1 Prupe.2G070300_v2.0.a1
pyrus_communis	pycom02g22750 pycom02g22760 pycom07g04140 pycom07g04160 pycom08g21330 pycom15g38880
rosa_chinensis	RchiOBHm_Chr1g0331011 RchiOBHm_Chr5g0049961
rosa_laevigata	RLG00000029759 RLG00000034672
rosa_multiflora	Rmu_sc0000333.1_g000038 Rmu_sc0013622.1_g000002 Rmu_sc0013622.1_g000003
rosa_roxburghii	Rroxscaffold_1G00031290 Rroxscaffold_4G00319550
rosa_rugosa	Rorug01G0091600 Rorug05G0255200
rosa_samantha	Rh1AG113300 Rh1BG087200 Rh1CG110200 Rh1DG118900 Rh5AG329100 Rh5BG339600 Rh5CG365600 Rh5DG352200
rosa_wichuraiana	Rw1G009240 Rw5G031070

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1785
AarI	CACCTGC	2 cut(s)	1190, 1384
Acc36I	ACCTGC	2 cut(s)	1190, 1384
Acc65I	GGTACC	2 cut(s)	959, 1075
AccB1I	GGYRCC	6 cut(s)	52, 380, 959, 1075, 1636, 1771
AccB7I	CCANNNNNTGG	2 cut(s)	377, 929
AccII	CGCG	1 cut(s)	892
AciI	CCGC	5 cut(s)	793, 939, 1308, 1540, 1802
AclWI	GGATC	6 cut(s)	14, 174, 581, 693, 784, 1700
AcuI	CTGAAG	3 cut(s)	743, 1164, 1826
AcyI	GRCGYC	3 cut(s)	53, 381, 1637
AfaI	GTAC	2 cut(s)	961, 1077
AfiI	CCNNNNNNNGG	6 cut(s)	362, 377, 633, 929, 932, 1715
AflIII	ACRYGT	2 cut(s)	768, 1623
AgsI	TTSAA	5 cut(s)	181, 474, 1007, 1042, 1261
AjnI	CCWGG	4 cut(s)	478, 873, 1404, 1657
AjuI	GAANNNNNNNTTGG	2 cut(s)	120, 152
AleI	CACNNNNGTG	1 cut(s)	634
AluBI	AGCT	9 cut(s)	77, 145, 254, 329, 799, 884, 1096, 1327, 1465
AluI	AGCT	9 cut(s)	77, 145, 254, 329, 799, 884, 1096, 1327, 1465
Alw26I	GTCTC	1 cut(s)	948
AlwI	GGATC	6 cut(s)	14, 174, 581, 693, 784, 1700
AoxI	GGCC	3 cut(s)	462, 638, 662
ApeKI	GCWGC	5 cut(s)	74, 98, 443, 577, 1549
ArsI	GACNNNNNNTTYG	4 cut(s)	1571, 1603, 1733, 1765
Asp700I	GAANNNNTTC	1 cut(s)	1175
Asp718I	GGTACC	2 cut(s)	959, 1075
AspA2I	CCTAGG	1 cut(s)	1219
AspLEI	GCGC	5 cut(s)	55, 383, 851, 892, 1639
AspS9I	GGNCC	3 cut(s)	437, 692, 1795
AsuC2I	CCSGG	1 cut(s)	1673
AsuHPI	GGTGA	7 cut(s)	80, 152, 751, 765, 1171, 1208, 1244
AsuNHI	GCTAGC	1 cut(s)	859
AvaII	GGWCC	3 cut(s)	437, 692, 1795
AvrII	CCTAGG	1 cut(s)	1219
BaeGI	GKGCMC	3 cut(s)	280, 681, 1202
BanI	GGYRCC	6 cut(s)	52, 380, 959, 1075, 1636, 1771
BbsI	GAAGAC	1 cut(s)	498
BbvI	GCAGC	5 cut(s)	86, 110, 430, 589, 1561
BccI	CCATC	3 cut(s)	64, 278, 307
BceAI	ACGGC	2 cut(s)	66, 768
BcgI	CGANNNNNNTGC	2 cut(s)	1220, 1254
BciT130I	CCWGG	4 cut(s)	480, 875, 1406, 1659
BclI	TGATCA	1 cut(s)	1687
BcnI	CCSGG	1 cut(s)	1673
BcoDI	GTCTC	1 cut(s)	948
BfaI	CTAG	2 cut(s)	860, 1220
BfmI	CTRYAG	3 cut(s)	496, 527, 1193
BfoI	RGCGCY	4 cut(s)	56, 384, 852, 1640
BfuAI	ACCTGC	2 cut(s)	1190, 1384
BisI	GCNGC	5 cut(s)	75, 99, 444, 578, 1550
BlnI	CCTAGG	1 cut(s)	1219
BlsI	GCNGC	5 cut(s)	76, 100, 445, 579, 1551
Bme1390I	CCNGG	5 cut(s)	480, 875, 1406, 1659, 1673
Bme18I	GGWCC	3 cut(s)	437, 692, 1795
BmgT120I	GGNCC	3 cut(s)	437, 692, 1795
BmiI	GGNNCC	7 cut(s)	54, 382, 693, 961, 1077, 1638, 1773
BmrFI	CCNGG	5 cut(s)	480, 875, 1406, 1659, 1673
BmsI	GCATC	5 cut(s)	61, 564, 1676, 1804, 1826
BmtI	GCTAGC	1 cut(s)	863
BpiI	GAAGAC	1 cut(s)	498
BplI	GAGNNNNNCTC	2 cut(s)	1094, 1126
BpmI	CTGGAG	1 cut(s)	63
Bpu10I	CCTNAGC	2 cut(s)	330, 840
BpuEI	CTTGAG	1 cut(s)	167
BpuMI	CCSGG	1 cut(s)	1673
BsaBI	GATNNNNATC	1 cut(s)	1103
BsaHI	GRCGYC	3 cut(s)	53, 381, 1637
BsaI	GGTCTC	1 cut(s)	948
BsaJI	CCNNGG	7 cut(s)	478, 479, 609, 632, 681, 1219, 1671
Bsc4I	CCNNNNNNNGG	6 cut(s)	362, 377, 633, 929, 932, 1715
Bse118I	RCCGGY	2 cut(s)	925, 1774
Bse1I	ACTGG	6 cut(s)	371, 649, 963, 1255, 1397, 1638
Bse3DI	GCAATG	1 cut(s)	1362
Bse8I	GATNNNNATC	1 cut(s)	1103
BseBI	CCWGG	4 cut(s)	480, 875, 1406, 1659
BseDI	CCNNGG	7 cut(s)	478, 479, 609, 632, 681, 1219, 1671
BseGI	GGATG	6 cut(s)	139, 162, 1609, 1654, 1667, 1817
BseJI	GATNNNNATC	1 cut(s)	1103
BseLI	CCNNNNNNNGG	6 cut(s)	362, 377, 633, 929, 932, 1715
BseMI	GCAATG	1 cut(s)	1362
BseMII	CTCAG	3 cut(s)	344, 1094, 1452
BseNI	ACTGG	6 cut(s)	371, 649, 963, 1255, 1397, 1638
BseRI	GAGGAG	3 cut(s)	122, 166, 507
BseSI	GKGCMC	3 cut(s)	280, 681, 1202
BseXI	GCAGC	5 cut(s)	86, 110, 430, 589, 1561
BsgI	GTGCAG	2 cut(s)	522, 1362
Bsh1236I	CGCG	1 cut(s)	892
BshFI	GGCC	3 cut(s)	464, 640, 664
BshNI	GGYRCC	6 cut(s)	52, 380, 959, 1075, 1636, 1771
BsiSI	CCGG	4 cut(s)	440, 926, 1673, 1775
BslFI	GGGAC	2 cut(s)	1443, 1741
BslI	CCNNNNNNNGG	6 cut(s)	362, 377, 633, 929, 932, 1715
BsmAI	GTCTC	1 cut(s)	948
BsmFI	GGGAC	2 cut(s)	1443, 1741
BsmI	GAATGC	1 cut(s)	177
BsnI	GGCC	3 cut(s)	464, 640, 664
Bso31I	GGTCTC	1 cut(s)	948
Bsp1286I	GDGCHC	3 cut(s)	280, 681, 1202
BspACI	CCGC	5 cut(s)	793, 939, 1308, 1540, 1802
BspANI	GGCC	3 cut(s)	464, 640, 664
BspCNI	CTCAG	3 cut(s)	343, 1093, 1453
BspFNI	CGCG	1 cut(s)	892
BspHI	TCATGA	2 cut(s)	715, 1207
BspLI	GGNNCC	7 cut(s)	54, 382, 693, 961, 1077, 1638, 1773
BspMAI	CTGCAG	2 cut(s)	500, 1197
BspMI	ACCTGC	2 cut(s)	1190, 1384
BspOI	GCTAGC	1 cut(s)	863
BspPI	GGATC	6 cut(s)	14, 174, 581, 693, 784, 1700
BspQI	GCTCTTC	1 cut(s)	1140
BspT107I	GGYRCC	6 cut(s)	52, 380, 959, 1075, 1636, 1771
BspTNI	GGTCTC	1 cut(s)	948
BsrDI	GCAATG	1 cut(s)	1362
BsrFI	RCCGGY	2 cut(s)	925, 1774
BsrI	ACTGG	6 cut(s)	371, 649, 963, 1255, 1397, 1638
BssAI	RCCGGY	2 cut(s)	925, 1774
BssECI	CCNNGG	7 cut(s)	478, 479, 609, 632, 681, 1219, 1671
BssNI	GRCGYC	3 cut(s)	53, 381, 1637
BssT1I	CCWWGG	4 cut(s)	609, 632, 681, 1219
Bst2UI	CCWGG	4 cut(s)	480, 875, 1406, 1659
Bst4CI	ACNGT	1 cut(s)	1484
Bst6I	CTCTTC	2 cut(s)	176, 1140
BstACI	GRCGYC	3 cut(s)	53, 381, 1637
BstC8I	GCNNGC	3 cut(s)	399, 797, 861
BstDEI	CTNAG	6 cut(s)	201, 330, 453, 840, 1080, 1461
BstENI	CCTNNNNNAGG	1 cut(s)	360
BstF5I	GGATG	6 cut(s)	139, 162, 1609, 1654, 1667, 1817
BstFNI	CGCG	1 cut(s)	892
BstH2I	RGCGCY	4 cut(s)	56, 384, 852, 1640
BstHHI	GCGC	5 cut(s)	55, 383, 851, 892, 1639
BstMAI	GTCTC	1 cut(s)	948
BstNI	CCWGG	4 cut(s)	480, 875, 1406, 1659
BstNSI	RCATGY	2 cut(s)	772, 1627
BstSCI	CCNGG	5 cut(s)	478, 873, 1404, 1657, 1671
BstSFI	CTRYAG	3 cut(s)	496, 527, 1193
BstSLI	GKGCMC	3 cut(s)	280, 681, 1202
BstUI	CGCG	1 cut(s)	892
BstV1I	GCAGC	5 cut(s)	86, 110, 430, 589, 1561
BstV2I	GAAGAC	1 cut(s)	498
BstX2I	RGATCY	2 cut(s)	6, 789
BstXI	CCANNNNNNTGG	1 cut(s)	636
BstYI	RGATCY	2 cut(s)	6, 789
BsuRI	GGCC	3 cut(s)	464, 640, 664
BtsCI	GGATG	6 cut(s)	139, 162, 1609, 1654, 1667, 1817
BtsI	GCAGTG	5 cut(s)	108, 505, 510, 580, 1202
BveI	ACCTGC	2 cut(s)	1190, 1384
Cac8I	GCNNGC	3 cut(s)	399, 797, 861
CciI	TCATGA	2 cut(s)	715, 1207
CfoI	GCGC	5 cut(s)	55, 383, 851, 892, 1639
Cfr10I	RCCGGY	2 cut(s)	925, 1774
Cfr13I	GGNCC	3 cut(s)	437, 692, 1795
CseI	GACGC	2 cut(s)	224, 1620
Csp6I	GTAC	2 cut(s)	960, 1076
CviAII	CATG	7 cut(s)	86, 402, 716, 769, 1208, 1624, 1696
CviQI	GTAC	2 cut(s)	960, 1076
DdeI	CTNAG	6 cut(s)	201, 330, 453, 840, 1080, 1461
DinI	GGCGCC	3 cut(s)	54, 382, 1638
Eam1104I	CTCTTC	2 cut(s)	176, 1140
EarI	CTCTTC	2 cut(s)	176, 1140
Eco130I	CCWWGG	4 cut(s)	609, 632, 681, 1219
Eco31I	GGTCTC	1 cut(s)	948
Eco47I	GGWCC	3 cut(s)	437, 692, 1795
Eco57I	CTGAAG	3 cut(s)	743, 1164, 1826
EcoNI	CCTNNNNNAGG	1 cut(s)	360
EcoO109I	RGGNCCY	1 cut(s)	692
EcoRII	CCWGG	4 cut(s)	478, 873, 1404, 1657
EcoT14I	CCWWGG	4 cut(s)	609, 632, 681, 1219
EcoT22I	ATGCAT	1 cut(s)	1819
EgeI	GGCGCC	3 cut(s)	54, 382, 1638
EheI	GGCGCC	3 cut(s)	54, 382, 1638
ErhI	CCWWGG	4 cut(s)	609, 632, 681, 1219
FaeI	CATG	7 cut(s)	89, 405, 719, 772, 1211, 1627, 1699
FaqI	GGGAC	2 cut(s)	1443, 1741
FatI	CATG	7 cut(s)	85, 401, 715, 768, 1207, 1623, 1695
FauI	CCCGC	1 cut(s)	932
FbaI	TGATCA	1 cut(s)	1687
Fnu4HI	GCNGC	5 cut(s)	75, 99, 444, 578, 1550
FokI	GGATG	6 cut(s)	146, 169, 1616, 1641, 1654, 1804
Fsp4HI	GCNGC	5 cut(s)	75, 99, 444, 578, 1550
FspBI	CTAG	2 cut(s)	860, 1220
GlaI	GCGC	5 cut(s)	54, 382, 850, 891, 1638
GluI	GCNGC	5 cut(s)	75, 99, 444, 578, 1550
GsuI	CTGGAG	1 cut(s)	63
HaeII	RGCGCY	4 cut(s)	56, 384, 852, 1640
HaeIII	GGCC	3 cut(s)	464, 640, 664
HapII	CCGG	4 cut(s)	440, 926, 1673, 1775
HgaI	GACGC	2 cut(s)	224, 1620
HhaI	GCGC	5 cut(s)	55, 383, 851, 892, 1639
Hin1I	GRCGYC	3 cut(s)	53, 381, 1637
Hin1II	CATG	7 cut(s)	89, 405, 719, 772, 1211, 1627, 1699
Hin6I	GCGC	5 cut(s)	53, 381, 849, 890, 1637
HinP1I	GCGC	5 cut(s)	53, 381, 849, 890, 1637
HincII	GTYRAC	2 cut(s)	1391, 1741
HindII	GTYRAC	2 cut(s)	1391, 1741
HindIII	AAGCTT	2 cut(s)	797, 882
HinfI	GANTC	3 cut(s)	288, 1350, 1754
HpaII	CCGG	4 cut(s)	440, 926, 1673, 1775
HphI	GGTGA	7 cut(s)	80, 152, 751, 765, 1171, 1208, 1244
Hpy166II	GTNNAC	5 cut(s)	557, 917, 1391, 1402, 1741
Hpy188III	TCNNGA	9 cut(s)	38, 80, 292, 716, 905, 1208, 1229, 1273, 1428
Hpy8I	GTNNAC	5 cut(s)	557, 917, 1391, 1402, 1741
HpyAV	CCTTC	5 cut(s)	59, 403, 721, 730, 888
HpyCH4III	ACNGT	1 cut(s)	1484
HpyCH4IV	ACGT	2 cut(s)	601, 975
HpyF3I	CTNAG	6 cut(s)	201, 330, 453, 840, 1080, 1461
HpySE526I	ACGT	2 cut(s)	601, 975
Hsp92I	GRCGYC	3 cut(s)	53, 381, 1637
Hsp92II	CATG	7 cut(s)	89, 405, 719, 772, 1211, 1627, 1699
HspAI	GCGC	5 cut(s)	53, 381, 849, 890, 1637
KasI	GGCGCC	3 cut(s)	52, 380, 1636
KpnI	GGTACC	2 cut(s)	963, 1079
Ksp22I	TGATCA	1 cut(s)	1687
LguI	GCTCTTC	1 cut(s)	1140
LmnI	GCTCC	1 cut(s)	82
Lsp1109I	GCAGC	5 cut(s)	86, 110, 430, 589, 1561
LweI	GCATC	5 cut(s)	61, 564, 1676, 1804, 1826
MaeI	CTAG	2 cut(s)	860, 1220
MaeII	ACGT	2 cut(s)	601, 975
MaeIII	GTNAC	4 cut(s)	240, 270, 771, 1718
MboII	GAAGA	8 cut(s)	137, 193, 274, 277, 496, 503, 736, 1157
MflI	RGATCY	2 cut(s)	6, 789
MhlI	GDGCHC	3 cut(s)	280, 681, 1202
MluCI	AATT	5 cut(s)	348, 447, 668, 1261, 1279
Mly113I	GGCGCC	3 cut(s)	53, 381, 1637
MlyI	GAGTC	1 cut(s)	1763
Mph1103I	ATGCAT	1 cut(s)	1819
MroXI	GAANNNNTTC	1 cut(s)	1175
MseI	TTAA	5 cut(s)	671, 1032, 1086, 1163, 1419
MslI	CAYNNNNRTG	2 cut(s)	634, 1622
MspA1I	CMGCKG	1 cut(s)	1804
MspI	CCGG	4 cut(s)	440, 926, 1673, 1775
MspR9I	CCNGG	5 cut(s)	480, 875, 1406, 1659, 1673
Mva1269I	GAATGC	1 cut(s)	177
MvaI	CCWGG	4 cut(s)	480, 875, 1406, 1659
MvnI	CGCG	1 cut(s)	892
NarI	GGCGCC	3 cut(s)	53, 381, 1637
NciI	CCSGG	1 cut(s)	1673
NheI	GCTAGC	1 cut(s)	859
NlaIII	CATG	7 cut(s)	89, 405, 719, 772, 1211, 1627, 1699
NlaIV	GGNNCC	7 cut(s)	54, 382, 693, 961, 1077, 1638, 1773
NmuCI	GTSAC	3 cut(s)	240, 270, 771
NsiI	ATGCAT	1 cut(s)	1819
NspI	RCATGY	2 cut(s)	772, 1627
OliI	CACNNNNGTG	1 cut(s)	634
PagI	TCATGA	2 cut(s)	715, 1207
PaqCI	CACCTGC	2 cut(s)	1190, 1384
PasI	CCCWGGG	1 cut(s)	479
PciI	ACATGT	2 cut(s)	768, 1623
PciSI	GCTCTTC	1 cut(s)	1140
PctI	GAATGC	1 cut(s)	177
PdmI	GAANNNNTTC	1 cut(s)	1175
PfeI	GAWTC	2 cut(s)	288, 1350
PflFI	GACNNNGTC	1 cut(s)	234
PflMI	CCANNNNNTGG	2 cut(s)	377, 929
PkrI	GCNGC	5 cut(s)	76, 100, 445, 579, 1551
PleI	GAGTC	1 cut(s)	1762
PluTI	GGCGCC	3 cut(s)	56, 384, 1640
PpsI	GAGTC	1 cut(s)	1762
PpuMI	RGGWCCY	1 cut(s)	692
PscI	ACATGT	2 cut(s)	768, 1623
PsiI	TTATAA	1 cut(s)	1785
Psp5II	RGGWCCY	1 cut(s)	692
Psp6I	CCWGG	4 cut(s)	478, 873, 1404, 1657
PspGI	CCWGG	4 cut(s)	478, 873, 1404, 1657
PspN4I	GGNNCC	7 cut(s)	54, 382, 693, 961, 1077, 1638, 1773
PspPI	GGNCC	3 cut(s)	437, 692, 1795
PspPPI	RGGWCCY	1 cut(s)	692
PstI	CTGCAG	2 cut(s)	500, 1197
PsuI	RGATCY	2 cut(s)	6, 789
PsyI	GACNNNGTC	1 cut(s)	234
RsaI	GTAC	2 cut(s)	961, 1077
RsaNI	GTAC	2 cut(s)	960, 1076
RseI	CAYNNNNRTG	2 cut(s)	634, 1622
SapI	GCTCTTC	1 cut(s)	1140
SaqAI	TTAA	5 cut(s)	671, 1032, 1086, 1163, 1419
SatI	GCNGC	5 cut(s)	75, 99, 444, 578, 1550
Sau96I	GGNCC	3 cut(s)	437, 692, 1795
SchI	GAGTC	1 cut(s)	1763
ScrFI	CCNGG	5 cut(s)	480, 875, 1406, 1659, 1673
SduI	GDGCHC	3 cut(s)	280, 681, 1202
SfaNI	GCATC	5 cut(s)	61, 564, 1676, 1804, 1826
SfcI	CTRYAG	3 cut(s)	496, 527, 1193
SfoI	GGCGCC	3 cut(s)	54, 382, 1638
SinI	GGWCC	3 cut(s)	437, 692, 1795
SmiMI	CAYNNNNRTG	2 cut(s)	634, 1622
SmlI	CTYRAG	1 cut(s)	146
SmoI	CTYRAG	1 cut(s)	146
Sse9I	AATT	5 cut(s)	348, 447, 668, 1261, 1279
SsiI	CCGC	5 cut(s)	793, 939, 1308, 1540, 1802
SspDI	GGCGCC	3 cut(s)	52, 380, 1636
SspI	AATATT	2 cut(s)	1174, 1489
SspMI	CTAG	2 cut(s)	860, 1220
StyD4I	CCNGG	5 cut(s)	478, 873, 1404, 1657, 1671
StyI	CCWWGG	4 cut(s)	609, 632, 681, 1219
TaaI	ACNGT	1 cut(s)	1484
TaiI	ACGT	2 cut(s)	604, 978
TaqI	TCGA	3 cut(s)	169, 1230, 1443
TasI	AATT	5 cut(s)	348, 447, 668, 1261, 1279
TfiI	GAWTC	2 cut(s)	288, 1350
Tru1I	TTAA	5 cut(s)	671, 1032, 1086, 1163, 1419
Tru9I	TTAA	5 cut(s)	671, 1032, 1086, 1163, 1419
TseFI	GTSAC	3 cut(s)	240, 270, 771
TseI	GCWGC	5 cut(s)	74, 98, 443, 577, 1549
Tsp45I	GTSAC	3 cut(s)	240, 270, 771
TspDTI	ATGAA	7 cut(s)	102, 138, 504, 1350, 1589, 1643, 1746
TspGWI	ACGGA	1 cut(s)	1810
Tth111I	GACNNNGTC	1 cut(s)	234
Van91I	CCANNNNNTGG	2 cut(s)	377, 929
VpaK11BI	GGWCC	3 cut(s)	437, 692, 1795
XagI	CCTNNNNNAGG	1 cut(s)	360
XceI	RCATGY	2 cut(s)	772, 1627
XmaJI	CCTAGG	1 cut(s)	1219
XmnI	GAANNNNTTC	1 cut(s)	1175
XspI	CTAG	2 cut(s)	860, 1220
Zsp2I	ATGCAT	1 cut(s)	1819

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.