Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0001963

Physical Location & Seq

Genomic Coordinates Reverse (-)

32030794 .. 32034292

Gene Structure 3499 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1266200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1821 bp

ATGGGGATCTTTGAAGAAATGGGTTTCTGTGGTAATCTTGACTTTCTTTCGGCTCCATCTGGGGAAGGCGAAGCAGCTCCAGAGCATGAACCTGAGGCAACTGTGGAGGAGGATTATAGTGATGAAGAAATGGATGTTGATGAGCTGGAGAGGAGGATGTGGAGATACCGAATGCTATTGAAGAGGCTGAAGGAACAAACTAAGGGAAAGGAAAGAGTTGACAATGCGAGACAGCGTCAATCACAGGAACAAGCTCGGAGGAAGAAGATGTCACGGGCACAAGATGGGATCCTGAAATATATGCTGAAAATGATGGAAGTTTGCAAAGCTCAGGGTTTTGTTTATGGAATTATCCCAGAAAAAGGAAAACCAGTGAGTGGCGCCTCAGACAATCTGCGAGCATGGTGGAAGGAAAAAGTAAGATTTGATCGTAATGGCCCGGCTGCAATTTCCAAGTATCAGGCAGATCATTCAATCCCTGGGAAGAATGAAGACTGCAGTGTAGTGGCATCCACTCCTCACACTTTACAGGAGCTGCAAGACACCACTCTTGGTTCACTTTTGTCGGCTTTGATGCAGCACTGTGATCCACCCCAAAGACGTTTCCCATTGGAGAAAGGTGTTGCCCCACCTTGGTGGCCTACTGGTAATGAGGATTGGTGGCCTCAATTAAATCTGCCCAAGGATCAGGGTCCTCCCCCATACAAGAAGCCTCATGATCTGAAGAAGGCTTGGAAGGTTGGCGTCCTCACAGCTGTGATAAAGCACATGTCACCAGATATTGCCAAGATCCGCAAGCTAGTTCGTCAGTCCAAGTGTTTGCAAGACAAAATGACTGCTAAGGAGAGCGCCACTTGGCTAGCTATTATAAACCAGGAGGAAGCTTTGGCCCGAAGGTTATATCCTGATAGATGTCCACCTCCACTGGCTGCTGGTGGTGGGTCTCTCACCATCAGTGGTACCAGTGATTATGATGTCGAAGGTGTTGATGATGACGAAAATGTTGAAACAGAGGATTGCAAACCTCTTGTAAATCACTTCAACATTGGAACCACTGGTCAAAGAGAGATGCTGGTACCTCAGATTAAGGGAGAGCTGATTGAGATCAACTCAGATTTTGGTCAAAAGAGGAAGCAGCTGTCTGAAGAGCCACAAATGATGTTAAATCAGAAAATTTACACCTGTGAATATCCACAGTGCCCGTATCATGATTATCGCCTAGGCTTTCTCAACATAACTGCAAGAAACAATCACCAGATGAACTGTCAATACCGCAGTAATTCTTCTCAAGTGTTTGGAATGTCAAGCTTTCAGCTTCACAATGAGAAATCTGTGGGCTACTCTCTACCCATTGCTCAACCACCTGCACCCACAATTCAACAATCAGTGAACCAGGCCAGTAGGTTTAATGCTTCGGGAGTTGATGATGGGCAGAAAATGATATCTGAGCTTATGTCATTCTATGATAGTAATGTTCAGCAGAACAAGAACTGCAATCCTGGAAACCTCCATATCGTAGATAACCGCAACCAGCAGCAGTCGAAATATCAATTGCCAATGAATGATAACTTTTTTGGTCAAGGGGTGGATACAGGATGCAACATCAACATGTCTGAACCGGCCCCCATGCCTATGCTTCATCCAGGTTTCGCATCCCAGGAAGTTCAGTTTGATCAGTGCATCGCATTTGATTCTCCATTTGGAAACAATCCCATTGAAAACGTCGACATGAGATTCGACTCCCCCTTGCACTTGGCACCAGCTGATTATAATGTTATGGACGCTCCGCTGAAGCAAGATGCACCCCCTTGGTTCCAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000160 GO:0000976 GO:0001067 GO:0001666 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0006950 GO:0006952 GO:0007154 GO:0007165 GO:0008150 GO:0009605 GO:0009607 GO:0009617 GO:0009628 GO:0009719 GO:0009723 GO:0009725 GO:0009743 GO:0009755 GO:0009756 GO:0009873 GO:0009889 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010039 GO:0010182 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0023052 GO:0031323 GO:0031326 GO:0032870 GO:0035556 GO:0036293 GO:0042221 GO:0042742 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044212 GO:0044424 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051252 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0065007 GO:0070482 GO:0070887 GO:0071241 GO:0071248 GO:0071281 GO:0071310 GO:0071322 GO:0071369 GO:0071495 GO:0080090 GO:0097159 GO:0098542 GO:0140110 GO:1901363 GO:1901700 GO:1901701 GO:1903506 GO:1990837 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

ko04016 ko04075 map04016 map04075

Pfam Domains

Protein Families

EIN3

Protein Analysis

607

Amino Acids

68.64

Weight (kDa)

5.53

Isoelectric Point (pI)

54.04

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
EIN3_DNA-bd	PF04873	47 - 294	1.7e-131	Ethylene insensitive 3, DNA-binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0001963)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G27050 AT3G20770
fragaria_vesca	FvH4_1g28720 FvH4_1g28720 FvH4_7g04940 FvH4_7g04940 FvH4_7g04940 FvH4_7g04940 FvH4_7g04940
malus_domestica	MD02G1266200.v1.1 MD02G1266300.v1.1 MD07G1053500.v1.1 MD07G1053800.v1.1 MD08G1245800.v1.1 MD15G1441000.v1.1
prunus_persica	Prupe.2G058400_v2.0.a1 Prupe.2G058500_v2.0.a1 Prupe.2G070300_v2.0.a1
pyrus_communis	pycom02g22750 pycom02g22760 pycom07g04140 pycom07g04160 pycom08g21330 pycom15g38880
rosa_chinensis	RchiOBHm_Chr1g0331011 RchiOBHm_Chr5g0049961
rosa_laevigata	RLG00000029759 RLG00000034672
rosa_multiflora	Rmu_sc0000333.1_g000038 Rmu_sc0013622.1_g000002 Rmu_sc0013622.1_g000003
rosa_roxburghii	Rroxscaffold_1G00031290 Rroxscaffold_4G00319550
rosa_rugosa	Rorug01G0091600 Rorug05G0255200
rosa_samantha	Rh1AG113300 Rh1BG087200 Rh1CG110200 Rh1DG118900 Rh5AG329100 Rh5BG339600 Rh5CG365600 Rh5DG352200
rosa_wichuraiana	Rw1G009240 Rw5G031070

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	869, 1770
AarI	CACCTGC	1 cut(s)	1372
Acc36I	ACCTGC	1 cut(s)	1372
Acc65I	GGTACC	2 cut(s)	959, 1075
AccB1I	GGYRCC	4 cut(s)	380, 959, 1075, 1756
AccB7I	CCANNNNNTGG	1 cut(s)	377
AccI	GTMKAC	1 cut(s)	1725
AciI	CCGC	4 cut(s)	793, 1273, 1525, 1787
AclWI	GGATC	6 cut(s)	14, 283, 296, 581, 693, 784
AcsI	RAATTY	1 cut(s)	1173
AcuI	CTGAAG	4 cut(s)	209, 743, 1164, 1811
AcyI	GRCGYC	2 cut(s)	381, 744
AfaI	GTAC	2 cut(s)	961, 1077
AfiI	CCNNNNNNNGG	3 cut(s)	362, 377, 633
AflIII	ACRYGT	2 cut(s)	768, 1608
AgsI	TTSAA	7 cut(s)	14, 181, 474, 1007, 1042, 1379, 1718
AjnI	CCWGG	6 cut(s)	478, 873, 1392, 1498, 1642, 1656
AleI	CACNNNNGTG	2 cut(s)	634, 755
Alw26I	GTCTC	2 cut(s)	223, 948
AlwI	GGATC	6 cut(s)	14, 283, 296, 581, 693, 784
AlwNI	CAGNNNCTG	1 cut(s)	535
AoxI	GGCC	6 cut(s)	436, 638, 662, 888, 1395, 1620
ApeKI	GCWGC	7 cut(s)	74, 443, 535, 577, 929, 1135, 1534
ApoI	RAATTY	1 cut(s)	1173
ArsI	GACNNNNNNTTYG	2 cut(s)	1718, 1750
Asp718I	GGTACC	2 cut(s)	959, 1075
AspA2I	CCTAGG	1 cut(s)	1219
AspLEI	GCGC	2 cut(s)	383, 851
AspS9I	GGNCC	4 cut(s)	437, 692, 889, 1621
AsuC2I	CCSGG	1 cut(s)	440
AsuHPI	GGTGA	3 cut(s)	765, 940, 1244
AsuNHI	GCTAGC	1 cut(s)	859
AvaII	GGWCC	1 cut(s)	692
AvrII	CCTAGG	1 cut(s)	1219
AxyI	CCTNAGG	1 cut(s)	93
BaeGI	GKGCMC	2 cut(s)	280, 1202
BamHI	GGATCC	1 cut(s)	288
BanI	GGYRCC	4 cut(s)	380, 959, 1075, 1756
BbsI	GAAGAC	1 cut(s)	498
BbvI	GCAGC	7 cut(s)	86, 430, 522, 589, 916, 1147, 1546
BccI	CCATC	5 cut(s)	64, 278, 307, 959, 1421
BciT130I	CCWGG	6 cut(s)	480, 875, 1394, 1500, 1644, 1658
BciVI	GTATCC	1 cut(s)	1582
BclI	TGATCA	1 cut(s)	1672
BcnI	CCSGG	1 cut(s)	440
BcoDI	GTCTC	2 cut(s)	223, 948
BfaI	CTAG	3 cut(s)	800, 860, 1220
BfmI	CTRYAG	1 cut(s)	496
BfoI	RGCGCY	2 cut(s)	384, 852
BfuAI	ACCTGC	1 cut(s)	1372
BfuI	GTATCC	1 cut(s)	1582
BisI	GCNGC	7 cut(s)	75, 444, 536, 578, 930, 1136, 1535
BlnI	CCTAGG	1 cut(s)	1219
BlsI	GCNGC	7 cut(s)	76, 445, 537, 579, 931, 1137, 1536
Bme1390I	CCNGG	7 cut(s)	440, 480, 875, 1394, 1500, 1644, 1658
Bme18I	GGWCC	1 cut(s)	692
BmgT120I	GGNCC	4 cut(s)	437, 692, 889, 1621
BmrFI	CCNGG	7 cut(s)	440, 480, 875, 1394, 1500, 1644, 1658
BmsI	GCATC	7 cut(s)	518, 564, 1059, 1586, 1661, 1689, 1789
BmtI	GCTAGC	1 cut(s)	863
BpiI	GAAGAC	1 cut(s)	498
BplI	GAGNNNNNCTC	2 cut(s)	1094, 1126
BpmI	CTGGAG	2 cut(s)	63, 167
Bpu10I	CCTNAGC	2 cut(s)	330, 840
BpuEI	CTTGAG	1 cut(s)	1272
BpuMI	CCSGG	1 cut(s)	440
BsaBI	GATNNNNATC	1 cut(s)	1103
BsaHI	GRCGYC	2 cut(s)	381, 744
BsaI	GGTCTC	1 cut(s)	948
BsaJI	CCNNGG	7 cut(s)	478, 479, 632, 681, 1219, 1656, 1808
Bsc4I	CCNNNNNNNGG	3 cut(s)	362, 377, 633
Bse118I	RCCGGY	1 cut(s)	1618
Bse1I	ACTGG	6 cut(s)	371, 649, 930, 963, 1060, 1398
Bse21I	CCTNAGG	1 cut(s)	93
Bse3DI	GCAATG	1 cut(s)	1350
Bse8I	GATNNNNATC	1 cut(s)	1103
BseBI	CCWGG	6 cut(s)	480, 875, 1394, 1500, 1644, 1658
BseDI	CCNNGG	7 cut(s)	478, 479, 632, 681, 1219, 1656, 1808
BseGI	GGATG	6 cut(s)	139, 162, 509, 1601, 1639, 1652
BseJI	GATNNNNATC	1 cut(s)	1103
BseLI	CCNNNNNNNGG	3 cut(s)	362, 377, 633
BseMI	GCAATG	1 cut(s)	1350
BseMII	CTCAG	6 cut(s)	84, 344, 399, 1094, 1125, 1437
BseNI	ACTGG	6 cut(s)	371, 649, 930, 963, 1060, 1398
BseRI	GAGGAG	3 cut(s)	122, 166, 507
BseSI	GKGCMC	2 cut(s)	280, 1202
BseXI	GCAGC	7 cut(s)	86, 430, 522, 589, 916, 1147, 1546
BsgI	GTGCAG	1 cut(s)	1350
BshFI	GGCC	6 cut(s)	438, 640, 664, 890, 1397, 1622
BshNI	GGYRCC	4 cut(s)	380, 959, 1075, 1756
BsiSI	CCGG	2 cut(s)	440, 1619
BslI	CCNNNNNNNGG	3 cut(s)	362, 377, 633
BsmAI	GTCTC	2 cut(s)	223, 948
BsmI	GAATGC	1 cut(s)	177
BsnI	GGCC	6 cut(s)	438, 640, 664, 890, 1397, 1622
Bso31I	GGTCTC	1 cut(s)	948
Bsp1286I	GDGCHC	2 cut(s)	280, 1202
BspACI	CCGC	4 cut(s)	793, 1273, 1525, 1787
BspANI	GGCC	6 cut(s)	438, 640, 664, 890, 1397, 1622
BspCNI	CTCAG	6 cut(s)	85, 343, 398, 1093, 1124, 1438
BspHI	TCATGA	2 cut(s)	715, 1207
BspMAI	CTGCAG	1 cut(s)	500
BspMI	ACCTGC	1 cut(s)	1372
BspOI	GCTAGC	1 cut(s)	863
BspPI	GGATC	6 cut(s)	14, 283, 296, 581, 693, 784
BspQI	GCTCTTC	1 cut(s)	1140
BspT107I	GGYRCC	4 cut(s)	380, 959, 1075, 1756
BspTNI	GGTCTC	1 cut(s)	948
BsrDI	GCAATG	1 cut(s)	1350
BsrFI	RCCGGY	1 cut(s)	1618
BsrI	ACTGG	6 cut(s)	371, 649, 930, 963, 1060, 1398
BssAI	RCCGGY	1 cut(s)	1618
BssECI	CCNNGG	7 cut(s)	478, 479, 632, 681, 1219, 1656, 1808
BssNI	GRCGYC	2 cut(s)	381, 744
BssT1I	CCWWGG	4 cut(s)	632, 681, 1219, 1808
Bst2UI	CCWGG	6 cut(s)	480, 875, 1394, 1500, 1644, 1658
Bst4CI	ACNGT	4 cut(s)	103, 584, 1197, 1265
Bst6I	CTCTTC	2 cut(s)	176, 1140
BstACI	GRCGYC	2 cut(s)	381, 744
BstC8I	GCNNGC	3 cut(s)	399, 797, 861
BstDEI	CTNAG	8 cut(s)	93, 201, 330, 385, 840, 1080, 1111, 1446
BstF5I	GGATG	6 cut(s)	139, 162, 509, 1601, 1639, 1652
BstH2I	RGCGCY	2 cut(s)	384, 852
BstHHI	GCGC	2 cut(s)	383, 851
BstMAI	GTCTC	2 cut(s)	223, 948
BstNI	CCWGG	6 cut(s)	480, 875, 1394, 1500, 1644, 1658
BstNSI	RCATGY	2 cut(s)	772, 1612
BstSCI	CCNGG	7 cut(s)	438, 478, 873, 1392, 1498, 1642, 1656
BstSFI	CTRYAG	1 cut(s)	496
BstSLI	GKGCMC	2 cut(s)	280, 1202
BstV1I	GCAGC	7 cut(s)	86, 430, 522, 589, 916, 1147, 1546
BstV2I	GAAGAC	1 cut(s)	498
BstX2I	RGATCY	3 cut(s)	6, 288, 789
BstXI	CCANNNNNNTGG	1 cut(s)	636
BstYI	RGATCY	3 cut(s)	6, 288, 789
Bsu36I	CCTNAGG	1 cut(s)	93
BsuI	GTATCC	1 cut(s)	1582
BsuRI	GGCC	6 cut(s)	438, 640, 664, 890, 1397, 1622
BtgZI	GCGATG	1 cut(s)	1666
BtsCI	GGATG	6 cut(s)	139, 162, 509, 1601, 1639, 1652
BtsI	GCAGTG	1 cut(s)	505
BveI	ACCTGC	1 cut(s)	1372
Cac8I	GCNNGC	3 cut(s)	399, 797, 861
CaiI	CAGNNNCTG	1 cut(s)	535
CciI	TCATGA	2 cut(s)	715, 1207
CfoI	GCGC	2 cut(s)	383, 851
Cfr10I	RCCGGY	1 cut(s)	1618
Cfr13I	GGNCC	4 cut(s)	437, 692, 889, 1621
CseI	GACGC	3 cut(s)	224, 733, 1790
Csp6I	GTAC	2 cut(s)	960, 1076
CviAII	CATG	8 cut(s)	86, 402, 716, 769, 1208, 1609, 1627, 1729
CviQI	GTAC	2 cut(s)	960, 1076
DdeI	CTNAG	8 cut(s)	93, 201, 330, 385, 840, 1080, 1111, 1446
DinI	GGCGCC	1 cut(s)	382
Eam1104I	CTCTTC	2 cut(s)	176, 1140
EarI	CTCTTC	2 cut(s)	176, 1140
Eco130I	CCWWGG	4 cut(s)	632, 681, 1219, 1808
Eco31I	GGTCTC	1 cut(s)	948
Eco32I	GATATC	1 cut(s)	1443
Eco47I	GGWCC	1 cut(s)	692
Eco57I	CTGAAG	4 cut(s)	209, 743, 1164, 1811
Eco81I	CCTNAGG	1 cut(s)	93
EcoO109I	RGGNCCY	1 cut(s)	692
EcoRII	CCWGG	6 cut(s)	478, 873, 1392, 1498, 1642, 1656
EcoRV	GATATC	1 cut(s)	1443
EcoT14I	CCWWGG	4 cut(s)	632, 681, 1219, 1808
EgeI	GGCGCC	1 cut(s)	382
EheI	GGCGCC	1 cut(s)	382
ErhI	CCWWGG	4 cut(s)	632, 681, 1219, 1808
FaeI	CATG	8 cut(s)	89, 405, 719, 772, 1211, 1612, 1630, 1732
FatI	CATG	8 cut(s)	85, 401, 715, 768, 1207, 1608, 1626, 1728
FbaI	TGATCA	1 cut(s)	1672
FblI	GTMKAC	1 cut(s)	1725
Fnu4HI	GCNGC	7 cut(s)	75, 444, 536, 578, 930, 1136, 1535
FokI	GGATG	6 cut(s)	146, 169, 496, 1608, 1626, 1639
Fsp4HI	GCNGC	7 cut(s)	75, 444, 536, 578, 930, 1136, 1535
FspBI	CTAG	3 cut(s)	800, 860, 1220
GlaI	GCGC	2 cut(s)	382, 850
GluI	GCNGC	7 cut(s)	75, 444, 536, 578, 930, 1136, 1535
GsuI	CTGGAG	2 cut(s)	63, 167
HaeII	RGCGCY	2 cut(s)	384, 852
HaeIII	GGCC	6 cut(s)	438, 640, 664, 890, 1397, 1622
HapII	CCGG	2 cut(s)	440, 1619
HgaI	GACGC	3 cut(s)	224, 733, 1790
HhaI	GCGC	2 cut(s)	383, 851
Hin1I	GRCGYC	2 cut(s)	381, 744
Hin1II	CATG	8 cut(s)	89, 405, 719, 772, 1211, 1612, 1630, 1732
Hin6I	GCGC	2 cut(s)	381, 849
HinP1I	GCGC	2 cut(s)	381, 849
HincII	GTYRAC	2 cut(s)	220, 1726
HindII	GTYRAC	2 cut(s)	220, 1726
HindIII	AAGCTT	2 cut(s)	882, 1306
HinfI	GANTC	3 cut(s)	1691, 1734, 1739
HpaII	CCGG	2 cut(s)	440, 1619
HphI	GGTGA	3 cut(s)	765, 940, 1244
Hpy166II	GTNNAC	5 cut(s)	220, 557, 917, 1390, 1726
Hpy188I	TCNGA	9 cut(s)	258, 388, 723, 1083, 1114, 1144, 1170, 1447, 1615
Hpy188III	TCNNGA	7 cut(s)	38, 80, 292, 716, 905, 1208, 1416
Hpy8I	GTNNAC	5 cut(s)	220, 557, 917, 1390, 1726
Hpy99I	CGWCG	1 cut(s)	1727
HpyAV	CCTTC	7 cut(s)	59, 184, 403, 721, 730, 888, 974
HpyCH4III	ACNGT	4 cut(s)	103, 584, 1197, 1265
HpyCH4IV	ACGT	2 cut(s)	601, 1722
HpyF3I	CTNAG	8 cut(s)	93, 201, 330, 385, 840, 1080, 1111, 1446
HpySE526I	ACGT	2 cut(s)	601, 1722
Hsp92I	GRCGYC	2 cut(s)	381, 744
Hsp92II	CATG	8 cut(s)	89, 405, 719, 772, 1211, 1612, 1630, 1732
HspAI	GCGC	2 cut(s)	381, 849
KasI	GGCGCC	1 cut(s)	380
KpnI	GGTACC	2 cut(s)	963, 1079
Ksp22I	TGATCA	1 cut(s)	1672
LguI	GCTCTTC	1 cut(s)	1140
LmnI	GCTCC	4 cut(s)	58, 82, 532, 1789
Lsp1109I	GCAGC	7 cut(s)	86, 430, 522, 589, 916, 1147, 1546
LweI	GCATC	7 cut(s)	518, 564, 1059, 1586, 1661, 1689, 1789
MaeI	CTAG	3 cut(s)	800, 860, 1220
MaeII	ACGT	2 cut(s)	601, 1722
MaeIII	GTNAC	2 cut(s)	270, 771
MfeI	CAATTG	1 cut(s)	1550
MflI	RGATCY	3 cut(s)	6, 288, 789
MhlI	GDGCHC	2 cut(s)	280, 1202
MluCI	AATT	7 cut(s)	348, 447, 668, 1173, 1279, 1374, 1550
Mly113I	GGCGCC	1 cut(s)	381
MlyI	GAGTC	1 cut(s)	1733
MseI	TTAA	4 cut(s)	671, 1086, 1163, 1407
MslI	CAYNNNNRTG	4 cut(s)	634, 755, 1607, 1631
MspA1I	CMGCKG	4 cut(s)	755, 1138, 1763, 1789
MspI	CCGG	2 cut(s)	440, 1619
MspR9I	CCNGG	7 cut(s)	440, 480, 875, 1394, 1500, 1644, 1658
MunI	CAATTG	1 cut(s)	1550
Mva1269I	GAATGC	1 cut(s)	177
MvaI	CCWGG	6 cut(s)	480, 875, 1394, 1500, 1644, 1658
NarI	GGCGCC	1 cut(s)	381
NciI	CCSGG	1 cut(s)	440
NheI	GCTAGC	1 cut(s)	859
NlaIII	CATG	8 cut(s)	89, 405, 719, 772, 1211, 1612, 1630, 1732
NmuCI	GTSAC	2 cut(s)	270, 771
NspI	RCATGY	2 cut(s)	772, 1612
OliI	CACNNNNGTG	2 cut(s)	634, 755
PagI	TCATGA	2 cut(s)	715, 1207
PaqCI	CACCTGC	1 cut(s)	1372
PasI	CCCWGGG	1 cut(s)	479
PciI	ACATGT	2 cut(s)	768, 1608
PciSI	GCTCTTC	1 cut(s)	1140
PctI	GAATGC	1 cut(s)	177
PfeI	GAWTC	2 cut(s)	1691, 1734
PflFI	GACNNNGTC	1 cut(s)	234
PflMI	CCANNNNNTGG	1 cut(s)	377
PfoI	TCCNGGA	1 cut(s)	1498
PkrI	GCNGC	7 cut(s)	76, 445, 537, 579, 931, 1137, 1536
PleI	GAGTC	1 cut(s)	1733
PluTI	GGCGCC	1 cut(s)	384
PpsI	GAGTC	1 cut(s)	1733
PpuMI	RGGWCCY	1 cut(s)	692
PscI	ACATGT	2 cut(s)	768, 1608
PsiI	TTATAA	2 cut(s)	869, 1770
Psp5II	RGGWCCY	1 cut(s)	692
Psp6I	CCWGG	6 cut(s)	478, 873, 1392, 1498, 1642, 1656
PspGI	CCWGG	6 cut(s)	478, 873, 1392, 1498, 1642, 1656
PspPI	GGNCC	4 cut(s)	437, 692, 889, 1621
PspPPI	RGGWCCY	1 cut(s)	692
PsrI	GAACNNNNNNTAC	2 cut(s)	1253, 1285
PstI	CTGCAG	1 cut(s)	500
PstNI	CAGNNNCTG	1 cut(s)	535
PsuI	RGATCY	3 cut(s)	6, 288, 789
PsyI	GACNNNGTC	1 cut(s)	234
PvuII	CAGCTG	3 cut(s)	755, 1138, 1763
RsaI	GTAC	2 cut(s)	961, 1077
RsaNI	GTAC	2 cut(s)	960, 1076
RseI	CAYNNNNRTG	4 cut(s)	634, 755, 1607, 1631
SalI	GTCGAC	1 cut(s)	1724
SapI	GCTCTTC	1 cut(s)	1140
SaqAI	TTAA	4 cut(s)	671, 1086, 1163, 1407
SatI	GCNGC	7 cut(s)	75, 444, 536, 578, 930, 1136, 1535
Sau96I	GGNCC	4 cut(s)	437, 692, 889, 1621
SchI	GAGTC	1 cut(s)	1733
ScrFI	CCNGG	7 cut(s)	440, 480, 875, 1394, 1500, 1644, 1658
SduI	GDGCHC	2 cut(s)	280, 1202
SfaNI	GCATC	7 cut(s)	518, 564, 1059, 1586, 1661, 1689, 1789
SfcI	CTRYAG	1 cut(s)	496
SfoI	GGCGCC	1 cut(s)	382
SinI	GGWCC	1 cut(s)	692
SmiMI	CAYNNNNRTG	4 cut(s)	634, 755, 1607, 1631
SmlI	CTYRAG	1 cut(s)	1287
SmoI	CTYRAG	1 cut(s)	1287
Sse9I	AATT	7 cut(s)	348, 447, 668, 1173, 1279, 1374, 1550
SsiI	CCGC	4 cut(s)	793, 1273, 1525, 1787
SspDI	GGCGCC	1 cut(s)	380
SspMI	CTAG	3 cut(s)	800, 860, 1220
StyD4I	CCNGG	7 cut(s)	438, 478, 873, 1392, 1498, 1642, 1656
StyI	CCWWGG	4 cut(s)	632, 681, 1219, 1808
TaaI	ACNGT	4 cut(s)	103, 584, 1197, 1265
TaiI	ACGT	2 cut(s)	604, 1725
TaqI	TCGA	4 cut(s)	978, 1541, 1725, 1737
TasI	AATT	7 cut(s)	348, 447, 668, 1173, 1279, 1374, 1550
TfiI	GAWTC	2 cut(s)	1691, 1734
Tru1I	TTAA	4 cut(s)	671, 1086, 1163, 1407
Tru9I	TTAA	4 cut(s)	671, 1086, 1163, 1407
TseFI	GTSAC	2 cut(s)	270, 771
TseI	GCWGC	7 cut(s)	74, 443, 535, 577, 929, 1135, 1534
Tsp45I	GTSAC	2 cut(s)	270, 771
TspDTI	ATGAA	6 cut(s)	102, 138, 504, 1274, 1574, 1628
Tth111I	GACNNNGTC	1 cut(s)	234
Van91I	CCANNNNNTGG	1 cut(s)	377
VpaK11BI	GGWCC	1 cut(s)	692
XapI	RAATTY	1 cut(s)	1173
XceI	RCATGY	2 cut(s)	772, 1612
XmaJI	CCTAGG	1 cut(s)	1219
XmiI	GTMKAC	1 cut(s)	1725
XspI	CTAG	3 cut(s)	800, 860, 1220

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.