AT2G28420

lactoylglutathione

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0003747
Physical Location & Seq
Forward (+)
12157613 .. 12158807
1195 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G28420.1

Sequence Viewer

Length: 555 bp
ATGGAGGAGAAGAAGAAGAAAGGCGATGATGAATTGAACTCGAAACCGCCGTTAATGGCGTTGAACCATGTGTCAAGGCTTTGTAAAGACGTCAAAAAGTCTCTCGAGTTCTACACAAAGGTATTAGGGTTCGTGGAGATAGAGCGTCCAGCTTCTTTCGACTTCGACGGTGCGTGGCTTTTCAACTACGGCGTTGGGATCCACTTGGTGCAGGCCAAGGACCAAGATAAGTTACCTTCTGACACGGACCATTTGGACCCAATGGATAACCACATCTCTTTCCAGTGTGAAGATATGGAAGCTTTGGAGAAGAGGCTCAAGGAGGTGAAGGTAAAGTACATCAAGAGGACGGTGGGTGATGAGAAAGATGCAGCTATCGACCAGCTCTTCTTCAATGACCCTGACGGCTTTATGGTTGAAATCTGTAACTGCGAGAATCTCGAACTTGTCCCGTGCCATTCAGCCGACGCCATACGCCTCCCAGAAGACCGACATGCACCTCCTGTTGCCCTCCCTGACTCCTCCAACCGTAGGATGCCCCAGCCCAATTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyoxalase

Protein Analysis

184

Amino Acids

21.0

Weight (kDa)

5.07

Isoelectric Point (pI)

45.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyoxalase PF00903 21 - 141 6.5e-13 Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily
Glyoxalase_2 PF12681 27 - 143 4.8e-06 Glyoxalase-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 93
AciI CCGC 1 cut(s) 47
AclWI GGATC 2 cut(s) 193, 206
AcyI GRCGYC 2 cut(s) 90, 468
AdeI CACNNNGTG 1 cut(s) 208
AfaI GTAC 1 cut(s) 338
AfiI CCNNNNNNNGG 1 cut(s) 531
AgsI TTSAA 5 cut(s) 37, 64, 184, 394, 419
AluBI AGCT 4 cut(s) 152, 302, 374, 385
AluI AGCT 4 cut(s) 152, 302, 374, 385
Alw26I GTCTC 1 cut(s) 105
AlwI GGATC 2 cut(s) 193, 206
Ama87I CYCGRG 1 cut(s) 104
AoxI GGCC 1 cut(s) 213
ApeKI GCWGC 1 cut(s) 371
AspS9I GGNCC 3 cut(s) 220, 247, 256
AsuHPI GGTGA 2 cut(s) 337, 368
AvaI CYCGRG 1 cut(s) 104
AvaII GGWCC 3 cut(s) 220, 247, 256
BamHI GGATCC 1 cut(s) 198
BarI GAAGNNNNNNTAC 2 cut(s) 320, 352
BbsI GAAGAC 1 cut(s) 492
BbvI GCAGC 1 cut(s) 383
BceAI ACGGC 3 cut(s) 34, 205, 421
BcoDI GTCTC 1 cut(s) 105
BisI GCNGC 1 cut(s) 372
BlsI GCNGC 1 cut(s) 373
Bme18I GGWCC 3 cut(s) 220, 247, 256
BmeT110I CYCGRG 1 cut(s) 104
BmgT120I GGNCC 3 cut(s) 220, 247, 256
BmiI GGNNCC 2 cut(s) 200, 258
BmsI GCATC 2 cut(s) 358, 525
BpiI GAAGAC 1 cut(s) 492
BpuEI CTTGAG 1 cut(s) 302
BsaHI GRCGYC 2 cut(s) 90, 468
BsaJI CCNNGG 1 cut(s) 216
Bsc4I CCNNNNNNNGG 1 cut(s) 531
Bse1I ACTGG 1 cut(s) 283
BseDI CCNNGG 1 cut(s) 216
BseGI GGATG 1 cut(s) 540
BseLI CCNNNNNNNGG 1 cut(s) 531
BseNI ACTGG 1 cut(s) 283
BseRI GAGGAG 2 cut(s) 20, 511
BseXI GCAGC 1 cut(s) 383
BseYI CCCAGC 1 cut(s) 540
BsgI GTGCAG 1 cut(s) 230
BshFI GGCC 1 cut(s) 215
BsiHKCI CYCGRG 1 cut(s) 104
BslFI GGGAC 1 cut(s) 434
BslI CCNNNNNNNGG 1 cut(s) 531
BsmAI GTCTC 1 cut(s) 105
BsmFI GGGAC 1 cut(s) 434
BsnI GGCC 1 cut(s) 215
BsoBI CYCGRG 1 cut(s) 104
Bsp143I GATC 1 cut(s) 198
BspACI CCGC 1 cut(s) 47
BspANI GGCC 1 cut(s) 215
BspLI GGNNCC 2 cut(s) 200, 258
BspPI GGATC 2 cut(s) 193, 206
BspQI GCTCTTC 1 cut(s) 392
BsrI ACTGG 1 cut(s) 283
BssECI CCNNGG 1 cut(s) 216
BssMI GATC 1 cut(s) 198
BssNI GRCGYC 2 cut(s) 90, 468
BssT1I CCWWGG 1 cut(s) 216
Bst4CI ACNGT 3 cut(s) 170, 352, 530
Bst6I CTCTTC 2 cut(s) 305, 392
BstACI GRCGYC 2 cut(s) 90, 468
BstC8I GCNNGC 1 cut(s) 213
BstF5I GGATG 1 cut(s) 540
BstKTI GATC 1 cut(s) 201
BstMAI GTCTC 1 cut(s) 105
BstMBI GATC 1 cut(s) 198
BstNSI RCATGY 1 cut(s) 497
BstV1I GCAGC 1 cut(s) 383
BstV2I GAAGAC 1 cut(s) 492
BstX2I RGATCY 1 cut(s) 198
BstYI RGATCY 1 cut(s) 198
BsuRI GGCC 1 cut(s) 215
BtgZI GCGATG 1 cut(s) 39
BtsCI GGATG 1 cut(s) 540
BtsIMutI CAGTG 1 cut(s) 290
Cac8I GCNNGC 1 cut(s) 213
Cfr13I GGNCC 3 cut(s) 220, 247, 256
CseI GACGC 2 cut(s) 134, 476
Csp6I GTAC 1 cut(s) 337
CviAII CATG 2 cut(s) 68, 494
CviQI GTAC 1 cut(s) 337
DpnI GATC 1 cut(s) 200
DpnII GATC 1 cut(s) 198
DraIII CACNNNGTG 1 cut(s) 208
Eam1104I CTCTTC 2 cut(s) 305, 392
EarI CTCTTC 2 cut(s) 305, 392
Eco130I CCWWGG 1 cut(s) 216
Eco47I GGWCC 3 cut(s) 220, 247, 256
Eco88I CYCGRG 1 cut(s) 104
EcoT14I CCWWGG 1 cut(s) 216
ErhI CCWWGG 1 cut(s) 216
FaeI CATG 2 cut(s) 71, 497
FaiI YATR 5 cut(s) 69, 296, 413, 473, 495
FaqI GGGAC 1 cut(s) 434
FatI CATG 2 cut(s) 67, 493
Fnu4HI GCNGC 1 cut(s) 372
FokI GGATG 1 cut(s) 547
Fsp4HI GCNGC 1 cut(s) 372
GluI GCNGC 1 cut(s) 372
GsaI CCCAGC 1 cut(s) 544
HaeIII GGCC 1 cut(s) 215
HgaI GACGC 2 cut(s) 134, 476
Hin1I GRCGYC 2 cut(s) 90, 468
Hin1II CATG 2 cut(s) 71, 497
HindIII AAGCTT 1 cut(s) 300
HinfI GANTC 2 cut(s) 436, 518
HphI GGTGA 2 cut(s) 337, 368
Hpy188I TCNGA 1 cut(s) 241
Hpy188III TCNNGA 3 cut(s) 104, 343, 440
Hpy99I CGWCG 2 cut(s) 170, 470
HpyAV CCTTC 2 cut(s) 246, 322
HpyCH4III ACNGT 3 cut(s) 170, 352, 530
HpyCH4IV ACGT 1 cut(s) 90
HpyCH4V TGCA 3 cut(s) 211, 371, 497
HpySE526I ACGT 1 cut(s) 90
Hsp92I GRCGYC 2 cut(s) 90, 468
Hsp92II CATG 2 cut(s) 71, 497
Kzo9I GATC 1 cut(s) 198
LguI GCTCTTC 1 cut(s) 392
LpnPI CCDG 8 cut(s) 162, 197, 296, 395, 414, 495, 516, 528
Lsp1109I GCAGC 1 cut(s) 383
LweI GCATC 2 cut(s) 358, 525
MaeII ACGT 1 cut(s) 90
MaeIII GTNAC 2 cut(s) 231, 425
MalI GATC 1 cut(s) 200
MboI GATC 1 cut(s) 198
MboII GAAGA 8 cut(s) 22, 25, 28, 302, 322, 379, 382, 497
MflI RGATCY 1 cut(s) 198
MluCI AATT 2 cut(s) 32, 547
MlyI GAGTC 1 cut(s) 512
MmeI TCCRAC 1 cut(s) 549
MnlI CCTC 7 cut(s) 306, 316, 339, 488, 510, 521, 532
MseI TTAA 2 cut(s) 53, 553
NdeII GATC 1 cut(s) 198
NlaIII CATG 2 cut(s) 71, 497
NlaIV GGNNCC 2 cut(s) 200, 258
NspI RCATGY 1 cut(s) 497
PaeR7I CTCGAG 1 cut(s) 104
PciSI GCTCTTC 1 cut(s) 392
PcsI WCGNNNNNNNCGW 1 cut(s) 47
PfeI GAWTC 1 cut(s) 436
PkrI GCNGC 1 cut(s) 373
PleI GAGTC 1 cut(s) 512
PpsI GAGTC 1 cut(s) 512
PspFI CCCAGC 1 cut(s) 540
PspN4I GGNNCC 2 cut(s) 200, 258
PspPI GGNCC 3 cut(s) 220, 247, 256
PsuI RGATCY 1 cut(s) 198
RsaI GTAC 1 cut(s) 338
RsaNI GTAC 1 cut(s) 337
SapI GCTCTTC 1 cut(s) 392
SaqAI TTAA 2 cut(s) 53, 553
SatI GCNGC 1 cut(s) 372
Sau3AI GATC 1 cut(s) 198
Sau96I GGNCC 3 cut(s) 220, 247, 256
SchI GAGTC 1 cut(s) 512
SfaNI GCATC 2 cut(s) 358, 525
Sfr274I CTCGAG 1 cut(s) 104
SinI GGWCC 3 cut(s) 220, 247, 256
SlaI CTCGAG 1 cut(s) 104
SmlI CTYRAG 2 cut(s) 104, 317
SmoI CTYRAG 2 cut(s) 104, 317
Sse9I AATT 2 cut(s) 32, 547
SsiI CCGC 1 cut(s) 47
StyI CCWWGG 1 cut(s) 216
TaaI ACNGT 3 cut(s) 170, 352, 530
TaiI ACGT 1 cut(s) 93
TaqI TCGA 6 cut(s) 41, 105, 159, 165, 378, 441
TaqII GACCGA 1 cut(s) 504
TasI AATT 2 cut(s) 32, 547
TatI WGTACW 1 cut(s) 336
TfiI GAWTC 1 cut(s) 436
Tru1I TTAA 2 cut(s) 53, 553
Tru9I TTAA 2 cut(s) 53, 553
TscAI CASTG 1 cut(s) 290
TseI GCWGC 1 cut(s) 371
TspDTI ATGAA 1 cut(s) 45
TspGWI ACGGA 1 cut(s) 260
TspRI CASTG 1 cut(s) 290
VpaK11BI GGWCC 3 cut(s) 220, 247, 256
XceI RCATGY 1 cut(s) 497
XhoI CTCGAG 1 cut(s) 104
ZraI GACGTC 1 cut(s) 91
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.