Rh1DG037200
ERF Family

Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0003747
Physical Location & Seq
Reverse (-)
6019912 .. 6020993
1082 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG037200.1

Sequence Viewer

Length: 624 bp
ATGTCTCAGATGCAGCAGATGAAGGAAGAAAATCTAATGGGCAAGAGAAGAAGTTATCTACCAACCAATAAGGAAAACCAACACAAAGAAAAGGATCATGAAAGGAAAGATCAAAAGGAAGAGCACCAACTTCCTCTAATGGCCTTGAATCATGTATCGAGGCTTTGTAGGAATGTGGAGGAGTCGGTAGATTTCTACACCAAAATGTTGGGGTTTATCAAGATTGAGAGGCCACCGGCTTTTGATTTCGACGGCGCTTGGCTGTTTAACTATGGAGTTGGGATTCACTTGGTGCAGTCCAAGGATAAGGATAGGCTCCCTGATCAAGATCATTTGGATCCCATGGACAACCACATCTCATTTCAGTGTGAGGACATGGATGCAATGGTGAAGAAATTGGAGGAGCTAAACATAGAGTACATGAAGAGGACAGTGGAGGATGATAAAAATGGGACAAAAATTGATCAAATCTTCTTCAATGACCCAGATGGATTCATGATTGAGATATGCAATTGTGAGAATCTCAAGCTTGTTCCTGCAGGTTCCTTGGGCAAAATAAAGCTGCCAATTGATAGGCATAATCCTCTTGCGGATATGGGTGACAATGGTACTGGTGCCAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyoxalase

Protein Analysis

207

Amino Acids

24.07

Weight (kDa)

5.39

Isoelectric Point (pI)

51.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyoxalase PF00903 49 - 169 1.3e-12 Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily
Glyoxalase_4 PF13669 50 - 154 5.6e-07 Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily
Glyoxalase_2 PF12681 55 - 171 3.8e-07 Glyoxalase-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 530
AccB1I GGYRCC 1 cut(s) 614
AciI CCGC 1 cut(s) 590
AclWI GGATC 3 cut(s) 102, 332, 345
AdeI CACNNNGTG 1 cut(s) 292
AfaI GTAC 2 cut(s) 419, 610
AgsI TTSAA 2 cut(s) 148, 478
AluBI AGCT 3 cut(s) 406, 529, 562
AluI AGCT 3 cut(s) 406, 529, 562
Alw21I GWGCWC 1 cut(s) 126
Alw26I GTCTC 1 cut(s) 9
AlwI GGATC 3 cut(s) 102, 332, 345
AoxI GGCC 2 cut(s) 141, 230
ApeKI GCWGC 2 cut(s) 13, 562
AspLEI GCGC 1 cut(s) 257
AsuHPI GGTGA 2 cut(s) 400, 611
BamHI GGATCC 1 cut(s) 337
BanI GGYRCC 1 cut(s) 614
Bbv12I GWGCWC 1 cut(s) 126
BbvI GCAGC 2 cut(s) 25, 549
BccI CCATC 1 cut(s) 482
BceAI ACGGC 1 cut(s) 268
BclI TGATCA 2 cut(s) 322, 463
BcoDI GTCTC 1 cut(s) 9
BfmI CTRYAG 1 cut(s) 537
BfoI RGCGCY 1 cut(s) 258
BfuAI ACCTGC 1 cut(s) 530
BisI GCNGC 2 cut(s) 14, 563
BlsI GCNGC 2 cut(s) 15, 564
BmiI GGNNCC 4 cut(s) 317, 339, 544, 616
BmsI GCATC 1 cut(s) 370
BpuEI CTTGAG 1 cut(s) 509
BsaBI GATNNNNATC 1 cut(s) 327
BsaJI CCNNGG 3 cut(s) 300, 342, 546
Bse118I RCCGGY 1 cut(s) 235
Bse1I ACTGG 1 cut(s) 616
Bse3DI GCAATG 1 cut(s) 390
Bse8I GATNNNNATC 1 cut(s) 327
BseDI CCNNGG 3 cut(s) 300, 342, 546
BseGI GGATG 2 cut(s) 385, 445
BseJI GATNNNNATC 1 cut(s) 327
BseMI GCAATG 1 cut(s) 390
BseMII CTCAG 1 cut(s) 20
BseNI ACTGG 1 cut(s) 616
BseRI GAGGAG 2 cut(s) 194, 416
BseXI GCAGC 2 cut(s) 25, 549
BsgI GTGCAG 1 cut(s) 314
BshFI GGCC 2 cut(s) 143, 232
BshNI GGYRCC 1 cut(s) 614
BsiHKAI GWGCWC 1 cut(s) 126
BsiSI CCGG 1 cut(s) 236
BslFI GGGAC 1 cut(s) 466
BsmAI GTCTC 1 cut(s) 9
BsmFI GGGAC 1 cut(s) 466
BsnI GGCC 2 cut(s) 143, 232
Bsp1286I GDGCHC 1 cut(s) 126
Bsp143I GATC 6 cut(s) 94, 109, 322, 328, 337, 463
Bsp19I CCATGG 1 cut(s) 342
BspACI CCGC 1 cut(s) 590
BspANI GGCC 2 cut(s) 143, 232
BspCNI CTCAG 1 cut(s) 19
BspHI TCATGA 2 cut(s) 97, 495
BspLI GGNNCC 4 cut(s) 317, 339, 544, 616
BspMAI CTGCAG 1 cut(s) 541
BspMI ACCTGC 1 cut(s) 530
BspPI GGATC 3 cut(s) 102, 332, 345
BspQI GCTCTTC 1 cut(s) 114
BspT107I GGYRCC 1 cut(s) 614
BsrDI GCAATG 1 cut(s) 390
BsrFI RCCGGY 1 cut(s) 235
BsrI ACTGG 1 cut(s) 616
BssAI RCCGGY 1 cut(s) 235
BssECI CCNNGG 3 cut(s) 300, 342, 546
BssMI GATC 6 cut(s) 94, 109, 322, 328, 337, 463
BssT1I CCWWGG 3 cut(s) 300, 342, 546
Bst4CI ACNGT 1 cut(s) 433
Bst6I CTCTTC 2 cut(s) 114, 419
BstDEI CTNAG 1 cut(s) 6
BstDSI CCRYGG 1 cut(s) 342
BstF5I GGATG 2 cut(s) 385, 445
BstH2I RGCGCY 1 cut(s) 258
BstHHI GCGC 1 cut(s) 257
BstKTI GATC 6 cut(s) 97, 112, 325, 331, 340, 466
BstMAI GTCTC 1 cut(s) 9
BstMBI GATC 6 cut(s) 94, 109, 322, 328, 337, 463
BstSFI CTRYAG 1 cut(s) 537
BstV1I GCAGC 2 cut(s) 25, 549
BstX2I RGATCY 1 cut(s) 337
BstXI CCANNNNNNTGG 1 cut(s) 208
BstYI RGATCY 1 cut(s) 337
BsuRI GGCC 2 cut(s) 143, 232
BtgI CCRYGG 1 cut(s) 342
BtsCI GGATG 2 cut(s) 385, 445
BtsIMutI CAGTG 2 cut(s) 371, 438
BveI ACCTGC 1 cut(s) 530
CciI TCATGA 2 cut(s) 97, 495
CfoI GCGC 1 cut(s) 257
Cfr10I RCCGGY 1 cut(s) 235
Csp6I GTAC 2 cut(s) 418, 609
CviAII CATG 6 cut(s) 98, 152, 343, 376, 421, 496
CviJI RGCY 9 cut(s) 143, 163, 232, 239, 262, 316, 406, 529, 562
CviKI_1 RGCY 9 cut(s) 143, 163, 232, 239, 262, 316, 406, 529, 562
CviQI GTAC 2 cut(s) 418, 609
DdeI CTNAG 1 cut(s) 6
DpnI GATC 6 cut(s) 96, 111, 324, 330, 339, 465
DpnII GATC 6 cut(s) 94, 109, 322, 328, 337, 463
DraIII CACNNNGTG 1 cut(s) 292
Eam1104I CTCTTC 2 cut(s) 114, 419
EarI CTCTTC 2 cut(s) 114, 419
Eco130I CCWWGG 3 cut(s) 300, 342, 546
EcoT14I CCWWGG 3 cut(s) 300, 342, 546
ErhI CCWWGG 3 cut(s) 300, 342, 546
FaeI CATG 6 cut(s) 101, 155, 346, 379, 424, 499
FaqI GGGAC 1 cut(s) 466
FatI CATG 6 cut(s) 97, 151, 342, 375, 420, 495
FbaI TGATCA 2 cut(s) 322, 463
Fnu4HI GCNGC 2 cut(s) 14, 563
FokI GGATG 2 cut(s) 392, 452
Fsp4HI GCNGC 2 cut(s) 14, 563
GlaI GCGC 1 cut(s) 256
GluI GCNGC 2 cut(s) 14, 563
HaeII RGCGCY 1 cut(s) 258
HaeIII GGCC 2 cut(s) 143, 232
HapII CCGG 1 cut(s) 236
HhaI GCGC 1 cut(s) 257
Hin1II CATG 6 cut(s) 101, 155, 346, 379, 424, 499
Hin6I GCGC 1 cut(s) 255
HinP1I GCGC 1 cut(s) 255
HindIII AAGCTT 1 cut(s) 527
HinfI GANTC 5 cut(s) 148, 182, 283, 492, 520
HpaII CCGG 1 cut(s) 236
HphI GGTGA 2 cut(s) 400, 611
Hpy188I TCNGA 1 cut(s) 9
Hpy188III TCNNGA 4 cut(s) 98, 220, 326, 496
Hpy99I CGWCG 1 cut(s) 254
HpyAV CCTTC 1 cut(s) 16
HpyCH4III ACNGT 1 cut(s) 433
HpyCH4V TGCA 5 cut(s) 13, 295, 383, 510, 539
HpyF3I CTNAG 1 cut(s) 6
Hsp92II CATG 6 cut(s) 101, 155, 346, 379, 424, 499
HspAI GCGC 1 cut(s) 255
Ksp22I TGATCA 2 cut(s) 322, 463
Kzo9I GATC 6 cut(s) 94, 109, 322, 328, 337, 463
LguI GCTCTTC 1 cut(s) 114
LmnI GCTCC 2 cut(s) 321, 403
LpnPI CCDG 6 cut(s) 249, 333, 498, 525, 549, 597
Lsp1109I GCAGC 2 cut(s) 25, 549
LweI GCATC 1 cut(s) 370
MaeIII GTNAC 1 cut(s) 599
MalI GATC 6 cut(s) 96, 111, 324, 330, 339, 465
MboI GATC 6 cut(s) 94, 109, 322, 328, 337, 463
MboII GAAGA 7 cut(s) 38, 60, 131, 403, 436, 463, 466
MfeI CAATTG 2 cut(s) 511, 567
MflI RGATCY 1 cut(s) 337
MhlI GDGCHC 1 cut(s) 126
MluCI AATT 4 cut(s) 395, 459, 511, 567
MlyI GAGTC 1 cut(s) 191
MnlI CCTC 9 cut(s) 144, 153, 172, 222, 364, 394, 420, 430, 594
MseI TTAA 1 cut(s) 267
MslI CAYNNNNRTG 2 cut(s) 203, 364
MspI CCGG 1 cut(s) 236
MunI CAATTG 2 cut(s) 511, 567
NcoI CCATGG 1 cut(s) 342
NdeII GATC 6 cut(s) 94, 109, 322, 328, 337, 463
NlaIII CATG 6 cut(s) 101, 155, 346, 379, 424, 499
NlaIV GGNNCC 4 cut(s) 317, 339, 544, 616
NmuCI GTSAC 1 cut(s) 599
PagI TCATGA 2 cut(s) 97, 495
PciSI GCTCTTC 1 cut(s) 114
PfeI GAWTC 4 cut(s) 148, 283, 492, 520
PkrI GCNGC 2 cut(s) 15, 564
PleI GAGTC 1 cut(s) 190
PpsI GAGTC 1 cut(s) 190
PspN4I GGNNCC 4 cut(s) 317, 339, 544, 616
PstI CTGCAG 1 cut(s) 541
PsuI RGATCY 1 cut(s) 337
RsaI GTAC 2 cut(s) 419, 610
RsaNI GTAC 2 cut(s) 418, 609
RseI CAYNNNNRTG 2 cut(s) 203, 364
SapI GCTCTTC 1 cut(s) 114
SaqAI TTAA 1 cut(s) 267
SatI GCNGC 2 cut(s) 14, 563
Sau3AI GATC 6 cut(s) 94, 109, 322, 328, 337, 463
SbfI CCTGCAGG 1 cut(s) 541
SchI GAGTC 1 cut(s) 191
SdaI CCTGCAGG 1 cut(s) 541
SduI GDGCHC 1 cut(s) 126
SetI ASST 4 cut(s) 408, 531, 544, 564
SfaNI GCATC 1 cut(s) 370
SfcI CTRYAG 1 cut(s) 537
SmiMI CAYNNNNRTG 2 cut(s) 203, 364
SmlI CTYRAG 1 cut(s) 524
SmoI CTYRAG 1 cut(s) 524
Sse8387I CCTGCAGG 1 cut(s) 541
Sse9I AATT 4 cut(s) 395, 459, 511, 567
SsiI CCGC 1 cut(s) 590
StyI CCWWGG 3 cut(s) 300, 342, 546
TaaI ACNGT 1 cut(s) 433
TaqI TCGA 2 cut(s) 158, 249
TasI AATT 4 cut(s) 395, 459, 511, 567
TatI WGTACW 1 cut(s) 417
TfiI GAWTC 4 cut(s) 148, 283, 492, 520
Tru1I TTAA 1 cut(s) 267
Tru9I TTAA 1 cut(s) 267
TscAI CASTG 2 cut(s) 371, 438
TseFI GTSAC 1 cut(s) 599
TseI GCWGC 2 cut(s) 13, 562
Tsp45I GTSAC 1 cut(s) 599
TspDTI ATGAA 4 cut(s) 35, 114, 437, 484
TspRI CASTG 2 cut(s) 371, 438
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.