RLG00000030535
ERF Family

Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0003747
Physical Location & Seq
Reverse (-)
63158108 .. 63158827
720 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000030535

Sequence Viewer

Length: 486 bp
ATGGCCTTGAATCATGTATCGAGGCTTTGTAGGAATGTGGAGGAGTCGGTAGATTTTTACACCAAAATGTTGGGGTTTGTCAAGATTGAGAGGCCACAAACTTTTGATTTCGACGGCGCTTGGCTGTTTAACTATGGAGTTGGGATTCACTTGGTGCAGTCCAAGGATAAGGATAGGCTCCCTGATCAAGATCATTTGGATCCCATGGACAACCACATCTCATTCCAGTGTGAGGACATGGATGCAATGGTGAAGAAATTGGAGGAGCTAAACATAGAGTACATGAAGAGGACAGTGGAGGATGATAAACATCGGACAAAAATTGATCAAATCTTCTTCAATGACCCAGATGGATTCATGATTGAGATATGCAATTGTGAGAATCTCAAGCTTGTTCCTGCAGGTTCGTTGGGCAAAATAAAGCTGCCAATTGATAGGCATAATCCTCCTGTGGATATGGTTGACAATGGTACTGATGCCAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyoxalase

Protein Analysis

162

Amino Acids

18.66

Weight (kDa)

4.97

Isoelectric Point (pI)

46.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyoxalase PF00903 3 - 123 3.4e-12 Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily
Glyoxalase_4 PF13669 4 - 105 7.2e-07 Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily
Glyoxalase_2 PF12681 9 - 125 1.8e-07 Glyoxalase-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 392
AclWI GGATC 2 cut(s) 194, 207
AdeI CACNNNGTG 1 cut(s) 154
AfaI GTAC 2 cut(s) 281, 472
AfiI CCNNNNNNNGG 1 cut(s) 232
AgsI TTSAA 2 cut(s) 10, 340
AluBI AGCT 3 cut(s) 268, 391, 424
AluI AGCT 3 cut(s) 268, 391, 424
AlwI GGATC 2 cut(s) 194, 207
AoxI GGCC 2 cut(s) 3, 92
ApeKI GCWGC 1 cut(s) 424
AspLEI GCGC 1 cut(s) 119
AsuHPI GGTGA 1 cut(s) 262
BamHI GGATCC 1 cut(s) 199
BbvI GCAGC 1 cut(s) 411
BccI CCATC 1 cut(s) 344
BceAI ACGGC 1 cut(s) 130
BclI TGATCA 2 cut(s) 184, 325
BfmI CTRYAG 1 cut(s) 399
BfoI RGCGCY 1 cut(s) 120
BfuAI ACCTGC 1 cut(s) 392
BisI GCNGC 1 cut(s) 425
BlsI GCNGC 1 cut(s) 426
BmiI GGNNCC 2 cut(s) 179, 201
BmsI GCATC 2 cut(s) 232, 466
BpuEI CTTGAG 1 cut(s) 371
BsaBI GATNNNNATC 2 cut(s) 189, 309
BsaJI CCNNGG 2 cut(s) 162, 204
Bsc4I CCNNNNNNNGG 1 cut(s) 232
Bse1I ACTGG 1 cut(s) 226
Bse3DI GCAATG 1 cut(s) 252
Bse8I GATNNNNATC 2 cut(s) 189, 309
BseDI CCNNGG 2 cut(s) 162, 204
BseGI GGATG 2 cut(s) 247, 307
BseJI GATNNNNATC 2 cut(s) 189, 309
BseLI CCNNNNNNNGG 1 cut(s) 232
BseMI GCAATG 1 cut(s) 252
BseNI ACTGG 1 cut(s) 226
BseRI GAGGAG 2 cut(s) 56, 278
BseXI GCAGC 1 cut(s) 411
BsgI GTGCAG 1 cut(s) 176
BshFI GGCC 2 cut(s) 5, 94
BslI CCNNNNNNNGG 1 cut(s) 232
BsnI GGCC 2 cut(s) 5, 94
Bsp143I GATC 4 cut(s) 184, 190, 199, 325
Bsp19I CCATGG 1 cut(s) 204
BspANI GGCC 2 cut(s) 5, 94
BspHI TCATGA 1 cut(s) 357
BspLI GGNNCC 2 cut(s) 179, 201
BspMAI CTGCAG 1 cut(s) 403
BspMI ACCTGC 1 cut(s) 392
BspPI GGATC 2 cut(s) 194, 207
BsrDI GCAATG 1 cut(s) 252
BsrI ACTGG 1 cut(s) 226
BssECI CCNNGG 2 cut(s) 162, 204
BssMI GATC 4 cut(s) 184, 190, 199, 325
BssT1I CCWWGG 2 cut(s) 162, 204
Bst4CI ACNGT 1 cut(s) 295
Bst6I CTCTTC 1 cut(s) 281
BstDSI CCRYGG 1 cut(s) 204
BstF5I GGATG 2 cut(s) 247, 307
BstH2I RGCGCY 1 cut(s) 120
BstHHI GCGC 1 cut(s) 119
BstKTI GATC 4 cut(s) 187, 193, 202, 328
BstMBI GATC 4 cut(s) 184, 190, 199, 325
BstSFI CTRYAG 1 cut(s) 399
BstV1I GCAGC 1 cut(s) 411
BstX2I RGATCY 1 cut(s) 199
BstXI CCANNNNNNTGG 1 cut(s) 70
BstYI RGATCY 1 cut(s) 199
BsuRI GGCC 2 cut(s) 5, 94
BtgI CCRYGG 1 cut(s) 204
BtsCI GGATG 2 cut(s) 247, 307
BtsIMutI CAGTG 2 cut(s) 233, 300
BveI ACCTGC 1 cut(s) 392
CciI TCATGA 1 cut(s) 357
CfoI GCGC 1 cut(s) 119
Csp6I GTAC 2 cut(s) 280, 471
CviAII CATG 5 cut(s) 14, 205, 238, 283, 358
CviJI RGCY 8 cut(s) 5, 25, 94, 124, 178, 268, 391, 424
CviKI_1 RGCY 8 cut(s) 5, 25, 94, 124, 178, 268, 391, 424
CviQI GTAC 2 cut(s) 280, 471
DpnI GATC 4 cut(s) 186, 192, 201, 327
DpnII GATC 4 cut(s) 184, 190, 199, 325
DraIII CACNNNGTG 1 cut(s) 154
Eam1104I CTCTTC 1 cut(s) 281
EarI CTCTTC 1 cut(s) 281
Eco130I CCWWGG 2 cut(s) 162, 204
EcoT14I CCWWGG 2 cut(s) 162, 204
ErhI CCWWGG 2 cut(s) 162, 204
FaeI CATG 5 cut(s) 17, 208, 241, 286, 361
FatI CATG 5 cut(s) 13, 204, 237, 282, 357
FbaI TGATCA 2 cut(s) 184, 325
Fnu4HI GCNGC 1 cut(s) 425
FokI GGATG 2 cut(s) 254, 314
Fsp4HI GCNGC 1 cut(s) 425
GlaI GCGC 1 cut(s) 118
GluI GCNGC 1 cut(s) 425
HaeII RGCGCY 1 cut(s) 120
HaeIII GGCC 2 cut(s) 5, 94
HhaI GCGC 1 cut(s) 119
Hin1II CATG 5 cut(s) 17, 208, 241, 286, 361
Hin6I GCGC 1 cut(s) 117
HinP1I GCGC 1 cut(s) 117
HincII GTYRAC 1 cut(s) 463
HindII GTYRAC 1 cut(s) 463
HindIII AAGCTT 1 cut(s) 389
HinfI GANTC 5 cut(s) 10, 44, 145, 354, 382
HphI GGTGA 1 cut(s) 262
Hpy166II GTNNAC 1 cut(s) 463
Hpy188I TCNGA 1 cut(s) 315
Hpy188III TCNNGA 3 cut(s) 82, 188, 358
Hpy8I GTNNAC 1 cut(s) 463
Hpy99I CGWCG 1 cut(s) 116
HpyCH4III ACNGT 1 cut(s) 295
HpyCH4V TGCA 4 cut(s) 157, 245, 372, 401
Hsp92II CATG 5 cut(s) 17, 208, 241, 286, 361
HspAI GCGC 1 cut(s) 117
Ksp22I TGATCA 2 cut(s) 184, 325
Kzo9I GATC 4 cut(s) 184, 190, 199, 325
LmnI GCTCC 2 cut(s) 183, 265
LpnPI CCDG 6 cut(s) 195, 239, 360, 387, 411, 462
Lsp1109I GCAGC 1 cut(s) 411
LweI GCATC 2 cut(s) 232, 466
MalI GATC 4 cut(s) 186, 192, 201, 327
MboI GATC 4 cut(s) 184, 190, 199, 325
MboII GAAGA 4 cut(s) 265, 298, 325, 328
MfeI CAATTG 2 cut(s) 373, 429
MflI RGATCY 1 cut(s) 199
MluCI AATT 4 cut(s) 257, 321, 373, 429
MlyI GAGTC 1 cut(s) 53
MnlI CCTC 8 cut(s) 15, 34, 84, 226, 256, 282, 292, 456
MseI TTAA 1 cut(s) 129
MslI CAYNNNNRTG 2 cut(s) 65, 226
MunI CAATTG 2 cut(s) 373, 429
NcoI CCATGG 1 cut(s) 204
NdeII GATC 4 cut(s) 184, 190, 199, 325
NlaIII CATG 5 cut(s) 17, 208, 241, 286, 361
NlaIV GGNNCC 2 cut(s) 179, 201
PagI TCATGA 1 cut(s) 357
PfeI GAWTC 4 cut(s) 10, 145, 354, 382
PkrI GCNGC 1 cut(s) 426
PleI GAGTC 1 cut(s) 52
PpsI GAGTC 1 cut(s) 52
PspN4I GGNNCC 2 cut(s) 179, 201
PstI CTGCAG 1 cut(s) 403
PsuI RGATCY 1 cut(s) 199
RsaI GTAC 2 cut(s) 281, 472
RsaNI GTAC 2 cut(s) 280, 471
RseI CAYNNNNRTG 2 cut(s) 65, 226
SaqAI TTAA 1 cut(s) 129
SatI GCNGC 1 cut(s) 425
Sau3AI GATC 4 cut(s) 184, 190, 199, 325
SbfI CCTGCAGG 1 cut(s) 403
SchI GAGTC 1 cut(s) 53
SdaI CCTGCAGG 1 cut(s) 403
SetI ASST 4 cut(s) 270, 393, 406, 426
SfaNI GCATC 2 cut(s) 232, 466
SfcI CTRYAG 1 cut(s) 399
SmiMI CAYNNNNRTG 2 cut(s) 65, 226
SmlI CTYRAG 1 cut(s) 386
SmoI CTYRAG 1 cut(s) 386
Sse8387I CCTGCAGG 1 cut(s) 403
Sse9I AATT 4 cut(s) 257, 321, 373, 429
StyI CCWWGG 2 cut(s) 162, 204
TaaI ACNGT 1 cut(s) 295
TaqI TCGA 2 cut(s) 20, 111
TasI AATT 4 cut(s) 257, 321, 373, 429
TatI WGTACW 1 cut(s) 279
TfiI GAWTC 4 cut(s) 10, 145, 354, 382
Tru1I TTAA 1 cut(s) 129
Tru9I TTAA 1 cut(s) 129
TscAI CASTG 2 cut(s) 233, 300
TseI GCWGC 1 cut(s) 424
TspDTI ATGAA 2 cut(s) 299, 346
TspRI CASTG 2 cut(s) 233, 300
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.