MD00G1106800.v1.1
ERF Family

Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0003747
Physical Location & Seq
Forward (+)
22438655 .. 22440725
2071 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1106800.v1.1.491

Sequence Viewer

Length: 525 bp
ATGAAAGAGAGCATGGGAAATCCGCTGCATTTGAAGTCCTTGAATCACATCTCGATAGCTTGCAGATCAGTTGAGAAATCCCTTGATTTCTACCAGAATGATCTAGGGTTCTTCCCAATCAGGAGGCCTGGCTCGTTAGATTTCACTGGTGCATTGCTATTCAATTATGGCATTGGCATCCATCTTCTCCAATCTGAAGACCCTGATAAAATTCCCAAGATCAGCCAGATTAATCCCAAAGATAACCACATTTCTTTCCAGTGTGAGAGCATGGCCTTAGTGGAGAAAAAGTTGAAGGAGATGCGGATCGAGTACGTGAAGCGCAGGGTGAAGGAGGGTGGGATCAACGTTGATCAGCTTTTCTTCCATGACCCAGATGGCACAATGATTGAGATCTGTAACTGTGACAACCTTCCCGTTATACCCCTAGAAAGCCAACCTATATCAGATCCTGCAGCACGTATCAGCTGCAGTGCTCATAAGAACAAGTGCCACACCAGATCATCAAACAAGCAGTCCAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyoxalase

Protein Analysis

175

Amino Acids

19.7

Weight (kDa)

8.2

Isoelectric Point (pI)

61.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyoxalase PF00903 14 - 132 5.9e-11 Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 23, 304
AclI AACGTT 1 cut(s) 348
AclWI GGATC 3 cut(s) 314, 350, 443
AcsI RAATTY 1 cut(s) 210
AcuI CTGAAG 1 cut(s) 216
AfaI GTAC 1 cut(s) 314
AgsI TTSAA 4 cut(s) 34, 43, 163, 295
AjnI CCWGG 1 cut(s) 127
AluBI AGCT 3 cut(s) 59, 358, 468
AluI AGCT 3 cut(s) 59, 358, 468
Alw21I GWGCWC 1 cut(s) 478
AlwI GGATC 3 cut(s) 314, 350, 443
AlwNI CAGNNNCTG 1 cut(s) 452
AoxI GGCC 2 cut(s) 125, 273
ApeKI GCWGC 3 cut(s) 25, 455, 468
ApoI RAATTY 1 cut(s) 210
ArsI GACNNNNNNTTYG 1 cut(s) 500
AseI ATTAAT 1 cut(s) 231
AspLEI GCGC 1 cut(s) 324
AsuHPI GGTGA 1 cut(s) 340
BbsI GAAGAC 1 cut(s) 204
Bbv12I GWGCWC 1 cut(s) 478
BbvI GCAGC 3 cut(s) 12, 455, 467
BccI CCATC 2 cut(s) 189, 371
BciT130I CCWGG 1 cut(s) 129
BclI TGATCA 1 cut(s) 352
BfaI CTAG 2 cut(s) 104, 428
BfmI CTRYAG 2 cut(s) 453, 469
BglII AGATCT 1 cut(s) 393
BisI GCNGC 3 cut(s) 26, 456, 469
BlsI GCNGC 3 cut(s) 27, 457, 470
Bme1390I CCNGG 1 cut(s) 129
BmrFI CCNGG 1 cut(s) 129
BmsI GCATC 2 cut(s) 186, 291
BpiI GAAGAC 1 cut(s) 204
BsaAI YACGTR 2 cut(s) 316, 461
BsaBI GATNNNNATC 2 cut(s) 305, 392
BsaXI ACNNNNNCTCC 2 cut(s) 275, 305
Bse1I ACTGG 2 cut(s) 151, 259
Bse3DI GCAATG 1 cut(s) 152
Bse8I GATNNNNATC 2 cut(s) 305, 392
BseBI CCWGG 1 cut(s) 129
BseGI GGATG 1 cut(s) 177
BseJI GATNNNNATC 2 cut(s) 305, 392
BseMI GCAATG 1 cut(s) 152
BseNI ACTGG 2 cut(s) 151, 259
BseXI GCAGC 3 cut(s) 12, 455, 467
BshFI GGCC 2 cut(s) 127, 275
BsiHKAI GWGCWC 1 cut(s) 478
BsnI GGCC 2 cut(s) 127, 275
Bsp1286I GDGCHC 1 cut(s) 478
Bsp143I GATC 9 cut(s) 65, 100, 219, 306, 342, 352, 393, 448, 500
BspACI CCGC 2 cut(s) 23, 304
BspANI GGCC 2 cut(s) 127, 275
BspMAI CTGCAG 2 cut(s) 457, 473
BspPI GGATC 3 cut(s) 314, 350, 443
BsrDI GCAATG 1 cut(s) 152
BsrI ACTGG 2 cut(s) 151, 259
BssMI GATC 9 cut(s) 65, 100, 219, 306, 342, 352, 393, 448, 500
Bst2UI CCWGG 1 cut(s) 129
Bst4CI ACNGT 1 cut(s) 404
BstBAI YACGTR 2 cut(s) 316, 461
BstC8I GCNNGC 1 cut(s) 61
BstDEI CTNAG 1 cut(s) 277
BstF5I GGATG 1 cut(s) 177
BstHHI GCGC 1 cut(s) 324
BstKTI GATC 9 cut(s) 68, 103, 222, 309, 345, 355, 396, 451, 503
BstMBI GATC 9 cut(s) 65, 100, 219, 306, 342, 352, 393, 448, 500
BstNI CCWGG 1 cut(s) 129
BstSCI CCNGG 1 cut(s) 127
BstSFI CTRYAG 2 cut(s) 453, 469
BstV1I GCAGC 3 cut(s) 12, 455, 467
BstV2I GAAGAC 1 cut(s) 204
BstX2I RGATCY 2 cut(s) 393, 448
BstYI RGATCY 2 cut(s) 393, 448
BsuRI GGCC 2 cut(s) 127, 275
BtsCI GGATG 1 cut(s) 177
BtsI GCAGTG 1 cut(s) 478
BtsIMutI CAGTG 3 cut(s) 144, 266, 478
Cac8I GCNNGC 1 cut(s) 61
CaiI CAGNNNCTG 1 cut(s) 452
CfoI GCGC 1 cut(s) 324
Csp6I GTAC 1 cut(s) 313
CviAII CATG 3 cut(s) 13, 271, 368
CviJI RGCY 8 cut(s) 59, 127, 132, 225, 275, 358, 435, 468
CviKI_1 RGCY 8 cut(s) 59, 127, 132, 225, 275, 358, 435, 468
CviQI GTAC 1 cut(s) 313
DdeI CTNAG 1 cut(s) 277
DpnI GATC 9 cut(s) 67, 102, 221, 308, 344, 354, 395, 450, 502
DpnII GATC 9 cut(s) 65, 100, 219, 306, 342, 352, 393, 448, 500
Eco147I AGGCCT 1 cut(s) 127
Eco57I CTGAAG 1 cut(s) 216
EcoRII CCWGG 1 cut(s) 127
FaeI CATG 3 cut(s) 16, 274, 371
FaiI YATR 7 cut(s) 14, 168, 272, 369, 422, 443, 480
FatI CATG 3 cut(s) 12, 270, 367
FbaI TGATCA 1 cut(s) 352
Fnu4HI GCNGC 3 cut(s) 26, 456, 469
FokI GGATG 1 cut(s) 164
Fsp4HI GCNGC 3 cut(s) 26, 456, 469
FspBI CTAG 2 cut(s) 104, 428
GlaI GCGC 1 cut(s) 323
GluI GCNGC 3 cut(s) 26, 456, 469
HaeIII GGCC 2 cut(s) 127, 275
HhaI GCGC 1 cut(s) 324
Hin1II CATG 3 cut(s) 16, 274, 371
Hin6I GCGC 1 cut(s) 322
HinP1I GCGC 1 cut(s) 322
HinfI GANTC 1 cut(s) 43
HphI GGTGA 1 cut(s) 340
Hpy188I TCNGA 2 cut(s) 196, 448
Hpy188III TCNNGA 2 cut(s) 52, 121
HpyAV CCTTC 3 cut(s) 289, 325, 422
HpyCH4III ACNGT 1 cut(s) 404
HpyCH4IV ACGT 3 cut(s) 315, 348, 460
HpyCH4V TGCA 5 cut(s) 28, 63, 152, 455, 471
HpyF3I CTNAG 1 cut(s) 277
HpySE526I ACGT 3 cut(s) 315, 348, 460
Hsp92II CATG 3 cut(s) 16, 274, 371
HspAI GCGC 1 cut(s) 322
Ksp22I TGATCA 1 cut(s) 352
Kzo9I GATC 9 cut(s) 65, 100, 219, 306, 342, 352, 393, 448, 500
Lsp1109I GCAGC 3 cut(s) 12, 455, 467
LweI GCATC 2 cut(s) 186, 291
MaeI CTAG 2 cut(s) 104, 428
MaeII ACGT 3 cut(s) 315, 348, 460
MaeIII GTNAC 2 cut(s) 398, 404
MalI GATC 9 cut(s) 67, 102, 221, 308, 344, 354, 395, 450, 502
MboI GATC 9 cut(s) 65, 100, 219, 306, 342, 352, 393, 448, 500
MboII GAAGA 4 cut(s) 103, 176, 209, 355
MflI RGATCY 2 cut(s) 393, 448
MhlI GDGCHC 1 cut(s) 478
MluCI AATT 2 cut(s) 163, 210
MnlI CCTC 2 cut(s) 117, 328
MseI TTAA 1 cut(s) 231
MspA1I CMGCKG 2 cut(s) 25, 468
MspR9I CCNGG 1 cut(s) 129
MvaI CCWGG 1 cut(s) 129
NdeII GATC 9 cut(s) 65, 100, 219, 306, 342, 352, 393, 448, 500
NlaIII CATG 3 cut(s) 16, 274, 371
NmuCI GTSAC 1 cut(s) 404
PceI AGGCCT 1 cut(s) 127
PfeI GAWTC 1 cut(s) 43
PkrI GCNGC 3 cut(s) 27, 457, 470
Ppu21I YACGTR 2 cut(s) 316, 461
PshBI ATTAAT 1 cut(s) 231
Psp1406I AACGTT 1 cut(s) 348
Psp6I CCWGG 1 cut(s) 127
PspGI CCWGG 1 cut(s) 127
PstI CTGCAG 2 cut(s) 457, 473
PstNI CAGNNNCTG 1 cut(s) 452
PsuI RGATCY 2 cut(s) 393, 448
PvuII CAGCTG 1 cut(s) 468
RsaI GTAC 1 cut(s) 314
RsaNI GTAC 1 cut(s) 313
SaqAI TTAA 1 cut(s) 231
SatI GCNGC 3 cut(s) 26, 456, 469
Sau3AI GATC 9 cut(s) 65, 100, 219, 306, 342, 352, 393, 448, 500
ScrFI CCNGG 1 cut(s) 129
SduI GDGCHC 1 cut(s) 478
SetI ASST 8 cut(s) 61, 318, 351, 360, 414, 442, 463, 470
SfaNI GCATC 2 cut(s) 186, 291
SfcI CTRYAG 2 cut(s) 453, 469
Sse9I AATT 2 cut(s) 163, 210
SseBI AGGCCT 1 cut(s) 127
SsiI CCGC 2 cut(s) 23, 304
SspMI CTAG 2 cut(s) 104, 428
StuI AGGCCT 1 cut(s) 127
StyD4I CCNGG 1 cut(s) 127
TaaI ACNGT 1 cut(s) 404
TaiI ACGT 3 cut(s) 318, 351, 463
TaqI TCGA 2 cut(s) 53, 309
TasI AATT 2 cut(s) 163, 210
TfiI GAWTC 1 cut(s) 43
Tru1I TTAA 1 cut(s) 231
Tru9I TTAA 1 cut(s) 231
TscAI CASTG 3 cut(s) 151, 266, 478
TseFI GTSAC 1 cut(s) 404
TseI GCWGC 3 cut(s) 25, 455, 468
Tsp45I GTSAC 1 cut(s) 404
TspDTI ATGAA 1 cut(s) 17
TspRI CASTG 3 cut(s) 151, 266, 478
VspI ATTAAT 1 cut(s) 231
XapI RAATTY 1 cut(s) 210
XcmI CCANNNNNNNNNTGG 1 cut(s) 374
XspI CTAG 2 cut(s) 104, 428
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.