FvH4_1g03551
ERF Family

Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0003747
Physical Location & Seq
Forward (+)
1942668 .. 1943620
953 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g03551.t1

Sequence Viewer

Length: 588 bp
ATGAAAGAAAGAACGGGAAATCCTCTGCAATTGAAGTCCTTGAATCACATCTCACTTGTTTGCAGGTCGCTCGAGAAATCCCTCGATTTCTACCAGAATGTTCTTGGCTTCTTTCCCGTTAGGAGGCCTGACTCCCTAGACTTCAATGGTGCATGGTTATTCAACTATGGCATTGGCATTCATCTTCTCCAAGCTGAAGACCCTGAGACGCTGCCCAAGATCAGCCACATTAACCCCAAAGATAACCACATTTCTTTCCAGTGTGAGAGCATGATCAGAGTGGAGAACAAGTTGAAGGAGATGGGGATCGAATACGTGACGAGCAGCGTAGAGGAAGGAGGAATCAACGTTGATCAGCTGTTCTTCCACGACCCGGATGGCACCATGATCGAGATCTGTAACTGTGACAACCTCCCAGTGATACCCCTAGTCGGAGATCAAACTCTCAGGTCTTGCACACGTGTGAAATGCAATGTGCAGCAGCAGCAGCAAGAAGCAATAGCACAGATGAAGCCTCAACAGCAAACCATGACCTGCATGCGTTCTAATGCTATACACATGAAGGAAGATTATCTTCCTTGTGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyoxalase

Protein Analysis

196

Amino Acids

22.24

Weight (kDa)

5.49

Isoelectric Point (pI)

57.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyoxalase PF00903 14 - 132 1.2e-12 Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily
Glyoxalase_4 PF13669 15 - 114 3.7e-06 Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily
Glyoxalase_2 PF12681 17 - 134 8.8e-06 Glyoxalase-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 54, 542
AccB1I GGYRCC 1 cut(s) 380
AclI AACGTT 1 cut(s) 348
AclWI GGATC 1 cut(s) 314
AcuI CTGAAG 1 cut(s) 216
AcvI CACGTG 1 cut(s) 461
AfiI CCNNNNNNNGG 3 cut(s) 123, 373, 431
AflIII ACRYGT 2 cut(s) 458, 460
AgsI TTSAA 5 cut(s) 34, 43, 145, 163, 295
AleI CACNNNNGTG 1 cut(s) 461
AloI GAACNNNNNNTCC 1 cut(s) 36
AluBI AGCT 2 cut(s) 194, 358
AluI AGCT 2 cut(s) 194, 358
Alw26I GTCTC 1 cut(s) 200
AlwI GGATC 1 cut(s) 314
Ama87I CYCGRG 1 cut(s) 71
AoxI GGCC 1 cut(s) 125
ApeKI GCWGC 6 cut(s) 211, 324, 478, 481, 484, 487
AsuC2I CCSGG 1 cut(s) 374
AvaI CYCGRG 1 cut(s) 71
BanI GGYRCC 1 cut(s) 380
BbrPI CACGTG 1 cut(s) 461
BbsI GAAGAC 1 cut(s) 204
BbvI GCAGC 6 cut(s) 198, 336, 490, 493, 496, 499
BccI CCATC 2 cut(s) 295, 371
BcgI CGANNNNNNTGC 4 cut(s) 52, 86, 370, 404
BclI TGATCA 2 cut(s) 273, 352
BcnI CCSGG 1 cut(s) 374
BcoDI GTCTC 1 cut(s) 200
BfaI CTAG 2 cut(s) 137, 428
BfuAI ACCTGC 2 cut(s) 54, 542
BglII AGATCT 1 cut(s) 393
BisI GCNGC 6 cut(s) 212, 325, 479, 482, 485, 488
BlsI GCNGC 6 cut(s) 213, 326, 480, 483, 486, 489
Bme1390I CCNGG 1 cut(s) 374
BmeT110I CYCGRG 1 cut(s) 71
BmiI GGNNCC 1 cut(s) 382
BmrFI CCNGG 1 cut(s) 374
BmrI ACTGGG 1 cut(s) 410
BmuI ACTGGG 1 cut(s) 410
BpiI GAAGAC 1 cut(s) 204
BpuMI CCSGG 1 cut(s) 374
BsaAI YACGTR 2 cut(s) 316, 461
BsaBI GATNNNNATC 2 cut(s) 305, 392
BsaXI ACNNNNNCTCC 2 cut(s) 275, 305
Bsc4I CCNNNNNNNGG 3 cut(s) 123, 373, 431
Bse1I ACTGG 2 cut(s) 259, 416
Bse3DI GCAATG 1 cut(s) 478
Bse8I GATNNNNATC 2 cut(s) 305, 392
BseGI GGATG 1 cut(s) 382
BseJI GATNNNNATC 2 cut(s) 305, 392
BseLI CCNNNNNNNGG 3 cut(s) 123, 373, 431
BseMI GCAATG 1 cut(s) 478
BseMII CTCAG 2 cut(s) 195, 460
BseNI ACTGG 2 cut(s) 259, 416
BseXI GCAGC 6 cut(s) 198, 336, 490, 493, 496, 499
BsgI GTGCAG 1 cut(s) 497
BshFI GGCC 1 cut(s) 127
BshNI GGYRCC 1 cut(s) 380
BsiHKCI CYCGRG 1 cut(s) 71
BsiSI CCGG 1 cut(s) 374
BslI CCNNNNNNNGG 3 cut(s) 123, 373, 431
BsmAI GTCTC 1 cut(s) 200
BsmBI CGTCTC 1 cut(s) 200
BsmI GAATGC 1 cut(s) 177
BsnI GGCC 1 cut(s) 127
BsoBI CYCGRG 1 cut(s) 71
Bsp143I GATC 7 cut(s) 219, 273, 306, 352, 387, 393, 436
BspANI GGCC 1 cut(s) 127
BspCNI CTCAG 2 cut(s) 196, 459
BspLI GGNNCC 1 cut(s) 382
BspMI ACCTGC 2 cut(s) 54, 542
BspPI GGATC 1 cut(s) 314
BspT107I GGYRCC 1 cut(s) 380
BsrDI GCAATG 1 cut(s) 478
BsrI ACTGG 2 cut(s) 259, 416
BssMI GATC 7 cut(s) 219, 273, 306, 352, 387, 393, 436
Bst4CI ACNGT 1 cut(s) 404
BstBAI YACGTR 2 cut(s) 316, 461
BstC8I GCNNGC 1 cut(s) 539
BstDEI CTNAG 2 cut(s) 204, 446
BstF5I GGATG 1 cut(s) 382
BstKTI GATC 7 cut(s) 222, 276, 309, 355, 390, 396, 439
BstMAI GTCTC 1 cut(s) 200
BstMBI GATC 7 cut(s) 219, 273, 306, 352, 387, 393, 436
BstMWI GCNNNNNNNGC 3 cut(s) 484, 487, 520
BstNSI RCATGY 1 cut(s) 541
BstSCI CCNGG 1 cut(s) 372
BstV1I GCAGC 6 cut(s) 198, 336, 490, 493, 496, 499
BstV2I GAAGAC 1 cut(s) 204
BstX2I RGATCY 1 cut(s) 393
BstYI RGATCY 1 cut(s) 393
BsuRI GGCC 1 cut(s) 127
BtsCI GGATG 1 cut(s) 382
BtsIMutI CAGTG 2 cut(s) 266, 423
BveI ACCTGC 2 cut(s) 54, 542
Cac8I GCNNGC 1 cut(s) 539
CseI GACGC 1 cut(s) 217
CviAII CATG 6 cut(s) 153, 271, 385, 529, 538, 559
CviJI RGCY 6 cut(s) 108, 127, 194, 225, 358, 514
CviKI_1 RGCY 6 cut(s) 108, 127, 194, 225, 358, 514
DdeI CTNAG 2 cut(s) 204, 446
DpnI GATC 7 cut(s) 221, 275, 308, 354, 389, 395, 438
DpnII GATC 7 cut(s) 219, 273, 306, 352, 387, 393, 436
Eco147I AGGCCT 1 cut(s) 127
Eco57I CTGAAG 1 cut(s) 216
Eco72I CACGTG 1 cut(s) 461
Eco88I CYCGRG 1 cut(s) 71
Esp3I CGTCTC 1 cut(s) 200
FaeI CATG 6 cut(s) 156, 274, 388, 532, 541, 562
FaiI YATR 8 cut(s) 154, 168, 272, 386, 530, 539, 554, 560
FalI AAGNNNNNCTT 1 cut(s) 558
FatI CATG 6 cut(s) 152, 270, 384, 528, 537, 558
FbaI TGATCA 2 cut(s) 273, 352
Fnu4HI GCNGC 6 cut(s) 212, 325, 479, 482, 485, 488
FokI GGATG 1 cut(s) 389
Fsp4HI GCNGC 6 cut(s) 212, 325, 479, 482, 485, 488
FspBI CTAG 2 cut(s) 137, 428
GluI GCNGC 6 cut(s) 212, 325, 479, 482, 485, 488
HaeIII GGCC 1 cut(s) 127
HapII CCGG 1 cut(s) 374
HgaI GACGC 1 cut(s) 217
Hin1II CATG 6 cut(s) 156, 274, 388, 532, 541, 562
HinfI GANTC 3 cut(s) 43, 131, 342
HpaII CCGG 1 cut(s) 374
Hpy188I TCNGA 2 cut(s) 278, 434
Hpy188III TCNNGA 2 cut(s) 73, 391
HpyAV CCTTC 3 cut(s) 289, 329, 556
HpyCH4III ACNGT 1 cut(s) 404
HpyCH4IV ACGT 3 cut(s) 315, 348, 460
HpyCH4V TGCA 7 cut(s) 28, 63, 152, 456, 471, 478, 537
HpyF10VI GCNNNNNNNGC 3 cut(s) 484, 487, 520
HpyF3I CTNAG 2 cut(s) 204, 446
HpySE526I ACGT 3 cut(s) 315, 348, 460
Hsp92II CATG 6 cut(s) 156, 274, 388, 532, 541, 562
Ksp22I TGATCA 2 cut(s) 273, 352
Kzo9I GATC 7 cut(s) 219, 273, 306, 352, 387, 393, 436
LpnPI CCDG 9 cut(s) 49, 107, 141, 216, 272, 387, 429, 433, 547
Lsp1109I GCAGC 6 cut(s) 198, 336, 490, 493, 496, 499
MaeI CTAG 2 cut(s) 137, 428
MaeII ACGT 3 cut(s) 315, 348, 460
MaeIII GTNAC 3 cut(s) 316, 398, 404
MalI GATC 7 cut(s) 221, 275, 308, 354, 389, 395, 438
MboI GATC 7 cut(s) 219, 273, 306, 352, 387, 393, 436
MboII GAAGA 5 cut(s) 176, 209, 355, 566, 578
MfeI CAATTG 1 cut(s) 29
MflI RGATCY 1 cut(s) 393
MluCI AATT 1 cut(s) 29
MlyI GAGTC 1 cut(s) 125
MmeI TCCRAC 1 cut(s) 412
MnlI CCTC 7 cut(s) 33, 92, 117, 325, 332, 422, 525
MseI TTAA 1 cut(s) 231
MslI CAYNNNNRTG 1 cut(s) 461
MspA1I CMGCKG 1 cut(s) 358
MspI CCGG 1 cut(s) 374
MspR9I CCNGG 1 cut(s) 374
MunI CAATTG 1 cut(s) 29
Mva1269I GAATGC 1 cut(s) 177
MwoI GCNNNNNNNGC 3 cut(s) 484, 487, 520
NciI CCSGG 1 cut(s) 374
NdeII GATC 7 cut(s) 219, 273, 306, 352, 387, 393, 436
NlaIII CATG 6 cut(s) 156, 274, 388, 532, 541, 562
NlaIV GGNNCC 1 cut(s) 382
NmuCI GTSAC 2 cut(s) 316, 404
NspI RCATGY 1 cut(s) 541
OliI CACNNNNGTG 1 cut(s) 461
PaeI GCATGC 1 cut(s) 541
PaeR7I CTCGAG 1 cut(s) 71
PceI AGGCCT 1 cut(s) 127
PctI GAATGC 1 cut(s) 177
PfeI GAWTC 2 cut(s) 43, 342
PkrI GCNGC 6 cut(s) 213, 326, 480, 483, 486, 489
PleI GAGTC 1 cut(s) 125
PmaCI CACGTG 1 cut(s) 461
PmlI CACGTG 1 cut(s) 461
PpsI GAGTC 1 cut(s) 125
Ppu21I YACGTR 2 cut(s) 316, 461
Psp1406I AACGTT 1 cut(s) 348
PspCI CACGTG 1 cut(s) 461
PspN4I GGNNCC 1 cut(s) 382
PsuI RGATCY 1 cut(s) 393
PvuII CAGCTG 1 cut(s) 358
RseI CAYNNNNRTG 1 cut(s) 461
SaqAI TTAA 1 cut(s) 231
SatI GCNGC 6 cut(s) 212, 325, 479, 482, 485, 488
Sau3AI GATC 7 cut(s) 219, 273, 306, 352, 387, 393, 436
SchI GAGTC 1 cut(s) 125
ScrFI CCNGG 1 cut(s) 374
SetI ASST 9 cut(s) 68, 196, 318, 351, 360, 414, 452, 463, 536
Sfr274I CTCGAG 1 cut(s) 71
SlaI CTCGAG 1 cut(s) 71
SmiMI CAYNNNNRTG 1 cut(s) 461
SmlI CTYRAG 1 cut(s) 71
SmoI CTYRAG 1 cut(s) 71
SphI GCATGC 1 cut(s) 541
Sse9I AATT 1 cut(s) 29
SseBI AGGCCT 1 cut(s) 127
SspMI CTAG 2 cut(s) 137, 428
StuI AGGCCT 1 cut(s) 127
StyD4I CCNGG 1 cut(s) 372
TaaI ACNGT 1 cut(s) 404
TaiI ACGT 3 cut(s) 318, 351, 463
TaqI TCGA 4 cut(s) 72, 84, 309, 390
TasI AATT 1 cut(s) 29
TfiI GAWTC 2 cut(s) 43, 342
Tru1I TTAA 1 cut(s) 231
Tru9I TTAA 1 cut(s) 231
TscAI CASTG 2 cut(s) 266, 423
TseFI GTSAC 2 cut(s) 316, 404
TseI GCWGC 6 cut(s) 211, 324, 478, 481, 484, 487
Tsp45I GTSAC 2 cut(s) 316, 404
TspDTI ATGAA 4 cut(s) 17, 170, 524, 575
TspRI CASTG 2 cut(s) 266, 423
XceI RCATGY 1 cut(s) 541
XcmI CCANNNNNNNNNTGG 2 cut(s) 101, 374
XhoI CTCGAG 1 cut(s) 71
XspI CTAG 2 cut(s) 137, 428
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.