Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0002254

Physical Location & Seq

Genomic Coordinates Forward (+)

13726781 .. 13731956

Gene Structure 5176 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT2G32320.3

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1578 bp

ATGGCGAATAGTAAGTACGAGTACGTGAAGTCATTCGAAGTAGAAGACGAAGTTATGTTTCCCAATCTGATAATTATCCGTATCGATGGCCGTGATTTTTCCAGATTTTCTCAAGTTCACAAGTTTGAAAAGCCTAATGATGAGACATCTCTAAATTTGATGAATTCATGTGCATCTTCGGTTTTAGTAGAGTATCCTGATATAGTCTTTGCATATGGATATAGTGACGAGTACAGCTTTGTATTCAAGAAAGCATCAAGATTCTACCAGAGACGAGCCAGTAAGATTCTGTCTTTGGTAGCCTCATTTTTTGCTGCGGTCTATGTAACGAAATGGAAAGAGTTCTTTCCTCATACAAAATTAGAATATGCTCCTTCATTTGCTTCAAAAGTTGTAAGTTGTGCATCGGTAGAGGTTCTTCAAGCTTACCTTGCGTGGAGACAACATGACTGCCACATCAGTAATCAGTATGACACATGTCTTTGGATGTTAGTAAAAAGTGGAAAGACCTTAAGTGAAACTCAGGAGATTTTGAAGGATACACAGAAACAACAGAGAAATGAGCTTCTTTTTCAACAATTTGGTATTAATTATAAAATGCTTCCTGTATTATTTCGTCAAGGATCATGCCTTTTTAAGACAAAGTTGGAAGAAACTGTAAAGCATGATGAGAATGGAAAGCCTGTAAAACGATTGAGGAGAAGAGAAACTTTAGTGCATTCAGAAAATGTTGCTGGAAGAAGCTTTTGGAATGAGCACTCCTCTCTTCATAAAGATCTTGGACATTTTGCAAAAGACATTGGCAAAATTGAACCAGATTATGTTAAGTCGTTTCAGTTTGAGAGTAGATTATTACCTTTAACTTGGGTTGTGGTTAGGATTGATGGCTGCCATTTTCACAGATTTTCTGAAGTTCATGAATTTGAGAAGCCAAATGATGAGCAAGCTCTGAAACTTATGAATTCATGTGCAGTGGCTGTTCTTGAGGAGTTTCAGGATATTGCCTTTGCCTACGGCGTTAGTGATGAGTTCAGCTTTGTTCTTAAGAATAAATCTGAGCTTTACAAAAGACAGTCCAGTAAGATAATATCTGCAGTAGTATCCTTCTTCACATCGACTTACATGATGAGATGGGGAGATTTTTTCCCTCACAAAAAATTGAAGTACCCTCCATCATTCGATGGACGAGCTGTTTGCTATCCAACATCAGATATTCTCCTGGATTATCTAGCTTGGAGACAAGTCGACTGCCATATCAACAATCAGTATAATACATGTTTCTGGATGCTTGTTAAGTCTGGAAAAAGCAAAATTCAAGCCCAGGATTACTTAAAGGGTACCCAGACACGAGAAAAAAACGAGTTACTTAGCCAGCAATTTGGAATTGAGTACAATTCATTACCTGTAATCTTCCGCATGGGTTCTTCCGTTTTCCGCCTAAAGACGCAGGAAGGTGTTACAGAGGAGAATGGAGAAGTTTCAGGAAAGCAAGTGGAAGCAGAAGTGGGCGTAGACTATTCAAACATTATCGACCAATGTTTCTGGCAACAACATCCTCACATTCTTAGCTTCTCCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000086 GO:0000166 GO:0000278 GO:0000287 GO:0000966 GO:0001882 GO:0001883 GO:0003674 GO:0003824 GO:0005488 GO:0005515 GO:0005524 GO:0005525 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005739 GO:0006139 GO:0006396 GO:0006399 GO:0006400 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0007049 GO:0008033 GO:0008144 GO:0008150 GO:0008152 GO:0008192 GO:0008193 GO:0008283 GO:0009451 GO:0009987 GO:0010467 GO:0016043 GO:0016070 GO:0016740 GO:0016772 GO:0016779 GO:0017076 GO:0019001 GO:0022402 GO:0022607 GO:0030554 GO:0031974 GO:0031981 GO:0032549 GO:0032550 GO:0032553 GO:0032555 GO:0032559 GO:0032561 GO:0033554 GO:0034470 GO:0034471 GO:0034641 GO:0034660 GO:0035639 GO:0036094 GO:0042802 GO:0043167 GO:0043168 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043412 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0044770 GO:0044772 GO:0044839 GO:0046483 GO:0046872 GO:0050896 GO:0051259 GO:0051260 GO:0051262 GO:0051289 GO:0051716 GO:0065003 GO:0070013 GO:0070568 GO:0071704 GO:0071840 GO:0090304 GO:0097159 GO:0097367 GO:0099116 GO:1901265 GO:1901360 GO:1901363 GO:1903047

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Thg1 Thg1C

Protein Analysis

525

Amino Acids

61.2

Weight (kDa)

7.58

Isoelectric Point (pI)

43.54

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Thg1	PF04446	6 - 133	7.1e-48	tRNAHis guanylyltransferase
Thg1C	PF14413	139 - 247	8.5e-30	Thg1 C terminal domain
Thg1	PF04446	280 - 408	2.4e-54	tRNAHis guanylyltransferase
Thg1C	PF14413	413 - 504	1.3e-35	Thg1 C terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002254)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G31580 AT2G31580 AT2G31580 AT2G31580 AT2G31580 AT2G32320 AT2G32320 AT2G32320
fragaria_vesca	FvH4_1g22971 FvH4_1g22971 FvH4_1g22971 FvH4_1g22971 FvH4_1g22971 FvH4_1g22971 FvH4_1g22971 FvH4_2g15830
malus_domestica	MD05G1030700.v1.1
prunus_persica	Prupe.1G573900_v2.0.a1
pyrus_communis	pycom05g02180 pycom10g02040
rosa_chinensis	RchiOBHm_Chr2g0117491 RchiOBHm_Chr2g0117501 RchiOBHm_Chr6g0279211
rosa_laevigata	RLG00000013155 RLG00000018350
rosa_multiflora	Rmu_co8223372.1_g000001 Rmu_sc0003454.1_g000019 Rmu_sc0003454.1_g000020 Rmu_sc0014203.1_g000009 Rmu_sc0014536.1_g000004 Rmu_sc0041068.1_g000002
rosa_roxburghii	Rroxscaffold_2G00126490 Rroxscaffold_7G00189030
rosa_rugosa	Rorug02G0212800 Rorug06G0123800
rosa_samantha	Rh2CG271800 Rh2DG275200 Rh2DG294700 Rh6AG232000 Rh6BG236900 Rh6CG238800 Rh6DG230400
rosa_wichuraiana	Rw6G020310

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	594
Acc65I	GGTACC	1 cut(s)	1337
AccB1I	GGYRCC	1 cut(s)	1337
AccI	GTMKAC	2 cut(s)	1245, 1512
AciI	CCGC	3 cut(s)	317, 1414, 1435
AclWI	GGATC	1 cut(s)	631
AcoI	YGGCCR	1 cut(s)	88
AcsI	RAATTY	5 cut(s)	154, 163, 920, 961, 1311
AcuI	CTGAAG	1 cut(s)	930
AfaI	GTAC	6 cut(s)	17, 23, 233, 1166, 1339, 1391
AflII	CTTAAG	2 cut(s)	511, 1043
AflIII	ACRYGT	2 cut(s)	476, 1274
AjnI	CCWGG	2 cut(s)	1218, 1320
AjuI	GAANNNNNNNTTGG	2 cut(s)	730, 762
Alw21I	GWGCWC	1 cut(s)	759
Alw26I	GTCTC	4 cut(s)	137, 265, 433, 1231
AlwI	GGATC	1 cut(s)	631
AlwNI	CAGNNNCTG	1 cut(s)	977
AoxI	GGCC	1 cut(s)	88
ApeKI	GCWGC	2 cut(s)	314, 888
ApoI	RAATTY	5 cut(s)	154, 163, 920, 961, 1311
ArsI	GACNNNNNNTTYG	2 cut(s)	1176, 1208
AseI	ATTAAT	1 cut(s)	588
Asp700I	GAANNNNTTC	2 cut(s)	32, 341
Asp718I	GGTACC	1 cut(s)	1337
AsuII	TTCGAA	1 cut(s)	36
BanI	GGYRCC	1 cut(s)	1337
BarI	GAAGNNNNNNTAC	2 cut(s)	402, 434
BauI	CACGAG	1 cut(s)	1347
BbsI	GAAGAC	1 cut(s)	51
Bbv12I	GWGCWC	1 cut(s)	759
BbvI	GCAGC	2 cut(s)	301, 875
BccI	CCATC	5 cut(s)	80, 878, 1125, 1175, 1180
BceAI	ACGGC	2 cut(s)	75, 1030
BcgI	CGANNNNNNTGC	2 cut(s)	1176, 1210
BciT130I	CCWGG	2 cut(s)	1220, 1322
BciVI	GTATCC	3 cut(s)	204, 532, 1111
BcoDI	GTCTC	4 cut(s)	137, 265, 433, 1231
BfaI	CTAG	1 cut(s)	1229
BfmI	CTRYAG	1 cut(s)	1092
BfrI	CTTAAG	2 cut(s)	511, 1043
BfuI	GTATCC	3 cut(s)	204, 532, 1111
BglII	AGATCT	1 cut(s)	775
BisI	GCNGC	2 cut(s)	315, 889
BlsI	GCNGC	2 cut(s)	316, 890
Bme1390I	CCNGG	2 cut(s)	1220, 1322
BmiI	GGNNCC	1 cut(s)	1339
BmrFI	CCNGG	2 cut(s)	1220, 1322
BmsI	GCATC	4 cut(s)	182, 263, 413, 1275
BoxI	GACNNNNGTC	1 cut(s)	477
BpiI	GAAGAC	1 cut(s)	51
BplI	GAGNNNNNCTC	2 cut(s)	746, 778
Bpu14I	TTCGAA	1 cut(s)	36
BpuEI	CTTGAG	2 cut(s)	96, 1004
Bsa29I	ATCGAT	1 cut(s)	84
BsaAI	YACGTR	1 cut(s)	25
BsaBI	GATNNNNATC	1 cut(s)	74
BsaJI	CCNNGG	1 cut(s)	1320
Bse1I	ACTGG	2 cut(s)	279, 1077
Bse8I	GATNNNNATC	1 cut(s)	74
BseBI	CCWGG	2 cut(s)	1220, 1322
BseCI	ATCGAT	1 cut(s)	84
BseDI	CCNNGG	1 cut(s)	1320
BseGI	GGATG	3 cut(s)	492, 1290, 1552
BseJI	GATNNNNATC	1 cut(s)	74
BseMII	CTCAG	2 cut(s)	536, 1047
BseNI	ACTGG	2 cut(s)	279, 1077
BseRI	GAGGAG	4 cut(s)	712, 751, 1001, 1478
BseXI	GCAGC	2 cut(s)	301, 875
BsgI	GTGCAG	1 cut(s)	990
BshFI	GGCC	1 cut(s)	90
BshNI	GGYRCC	1 cut(s)	1337
BshVI	ATCGAT	1 cut(s)	84
BsiHKAI	GWGCWC	1 cut(s)	759
BsmAI	GTCTC	4 cut(s)	137, 265, 433, 1231
BsmBI	CGTCTC	1 cut(s)	265
BsmI	GAATGC	1 cut(s)	718
BsnI	GGCC	1 cut(s)	90
Bsp119I	TTCGAA	1 cut(s)	36
Bsp1286I	GDGCHC	1 cut(s)	759
Bsp143I	GATC	2 cut(s)	623, 775
BspACI	CCGC	3 cut(s)	317, 1414, 1435
BspANI	GGCC	1 cut(s)	90
BspCNI	CTCAG	2 cut(s)	535, 1048
BspDI	ATCGAT	1 cut(s)	84
BspHI	TCATGA	1 cut(s)	916
BspLI	GGNNCC	1 cut(s)	1339
BspMAI	CTGCAG	1 cut(s)	1096
BspPI	GGATC	1 cut(s)	631
BspT104I	TTCGAA	1 cut(s)	36
BspT107I	GGYRCC	1 cut(s)	1337
BspTI	CTTAAG	2 cut(s)	511, 1043
BsrI	ACTGG	2 cut(s)	279, 1077
BssECI	CCNNGG	1 cut(s)	1320
BssMI	GATC	2 cut(s)	623, 775
BssSI	CACGAG	1 cut(s)	1347
Bst2BI	CACGAG	1 cut(s)	1347
Bst2UI	CCWGG	2 cut(s)	1220, 1322
Bst4CI	ACNGT	2 cut(s)	658, 1074
Bst6I	CTCTTC	2 cut(s)	697, 771
BstAFI	CTTAAG	2 cut(s)	511, 1043
BstBAI	YACGTR	1 cut(s)	25
BstBI	TTCGAA	1 cut(s)	36
BstC8I	GCNNGC	2 cut(s)	945, 1373
BstDEI	CTNAG	4 cut(s)	522, 1056, 1367, 1565
BstF5I	GGATG	3 cut(s)	492, 1290, 1552
BstKTI	GATC	2 cut(s)	626, 778
BstMAI	GTCTC	4 cut(s)	137, 265, 433, 1231
BstMBI	GATC	2 cut(s)	623, 775
BstMWI	GCNNNNNNNGC	1 cut(s)	431
BstNI	CCWGG	2 cut(s)	1220, 1322
BstNSI	RCATGY	2 cut(s)	480, 1278
BstPAI	GACNNNNGTC	1 cut(s)	477
BstSCI	CCNGG	2 cut(s)	1218, 1320
BstSFI	CTRYAG	1 cut(s)	1092
BstV1I	GCAGC	2 cut(s)	301, 875
BstV2I	GAAGAC	1 cut(s)	51
BstX2I	RGATCY	1 cut(s)	775
BstXI	CCANNNNNNTGG	1 cut(s)	1379
BstYI	RGATCY	1 cut(s)	775
Bsu15I	ATCGAT	1 cut(s)	84
BsuI	GTATCC	3 cut(s)	204, 532, 1111
BsuRI	GGCC	1 cut(s)	90
BsuTUI	ATCGAT	1 cut(s)	84
BtsCI	GGATG	3 cut(s)	492, 1290, 1552
BtsI	GCAGTG	1 cut(s)	978
BtsIMutI	CAGTG	1 cut(s)	978
Cac8I	GCNNGC	2 cut(s)	945, 1373
CaiI	CAGNNNCTG	1 cut(s)	977
CciI	TCATGA	1 cut(s)	916
ClaI	ATCGAT	1 cut(s)	84
CseI	GACGC	1 cut(s)	1453
Csp6I	GTAC	6 cut(s)	16, 22, 232, 1165, 1338, 1390
CviQI	GTAC	6 cut(s)	16, 22, 232, 1165, 1338, 1390
DdeI	CTNAG	4 cut(s)	522, 1056, 1367, 1565
DpnI	GATC	2 cut(s)	625, 777
DpnII	GATC	2 cut(s)	623, 775
EaeI	YGGCCR	1 cut(s)	88
Eam1104I	CTCTTC	2 cut(s)	697, 771
EarI	CTCTTC	2 cut(s)	697, 771
EciI	GGCGGA	1 cut(s)	1424
Eco57I	CTGAAG	1 cut(s)	930
EcoRI	GAATTC	2 cut(s)	163, 961
EcoRII	CCWGG	2 cut(s)	1218, 1320
Esp3I	CGTCTC	1 cut(s)	265
FalI	AAGNNNNNCTT	4 cut(s)	414, 446, 694, 726
FauNDI	CATATG	1 cut(s)	214
FblI	GTMKAC	2 cut(s)	1245, 1512
Fnu4HI	GCNGC	2 cut(s)	315, 889
FokI	GGATG	3 cut(s)	499, 1297, 1539
Fsp4HI	GCNGC	2 cut(s)	315, 889
FspBI	CTAG	1 cut(s)	1229
GluI	GCNGC	2 cut(s)	315, 889
HaeIII	GGCC	1 cut(s)	90
HgaI	GACGC	1 cut(s)	1453
HincII	GTYRAC	1 cut(s)	1246
HindII	GTYRAC	1 cut(s)	1246
HindIII	AAGCTT	2 cut(s)	423, 742
HinfI	GANTC	2 cut(s)	261, 286
Hpy166II	GTNNAC	3 cut(s)	118, 1246, 1513
Hpy188I	TCNGA	6 cut(s)	69, 724, 910, 951, 1057, 1210
Hpy8I	GTNNAC	3 cut(s)	118, 1246, 1513
HpyAV	CCTTC	4 cut(s)	384, 529, 1114, 1445
HpyCH4III	ACNGT	2 cut(s)	658, 1074
HpyCH4IV	ACGT	1 cut(s)	24
HpyCH4V	TGCA	7 cut(s)	173, 212, 404, 718, 791, 971, 1094
HpyF10VI	GCNNNNNNNGC	1 cut(s)	431
HpyF3I	CTNAG	4 cut(s)	522, 1056, 1367, 1565
HpySE526I	ACGT	1 cut(s)	24
KpnI	GGTACC	1 cut(s)	1341
Kzo9I	GATC	2 cut(s)	623, 775
LmnI	GCTCC	1 cut(s)	376
Lsp1109I	GCAGC	2 cut(s)	301, 875
LweI	GCATC	4 cut(s)	182, 263, 413, 1275
MaeI	CTAG	1 cut(s)	1229
MaeII	ACGT	1 cut(s)	24
MaeIII	GTNAC	4 cut(s)	224, 325, 1362, 1456
MalI	GATC	2 cut(s)	625, 777
MboI	GATC	2 cut(s)	623, 775
MflI	RGATCY	1 cut(s)	775
MhlI	GDGCHC	1 cut(s)	759
MmeI	TCCRAC	2 cut(s)	627, 1226
MroXI	GAANNNNTTC	2 cut(s)	32, 341
MseI	TTAA	8 cut(s)	512, 588, 636, 825, 860, 1044, 1293, 1331
MspCI	CTTAAG	2 cut(s)	511, 1043
MspR9I	CCNGG	2 cut(s)	1220, 1322
Mva1269I	GAATGC	1 cut(s)	718
MvaI	CCWGG	2 cut(s)	1220, 1322
MwoI	GCNNNNNNNGC	1 cut(s)	431
NdeI	CATATG	1 cut(s)	214
NdeII	GATC	2 cut(s)	623, 775
NlaIV	GGNNCC	1 cut(s)	1339
NmuCI	GTSAC	1 cut(s)	224
NspI	RCATGY	2 cut(s)	480, 1278
NspV	TTCGAA	1 cut(s)	36
PagI	TCATGA	1 cut(s)	916
PciI	ACATGT	2 cut(s)	476, 1274
PctI	GAATGC	1 cut(s)	718
PdmI	GAANNNNTTC	2 cut(s)	32, 341
PfeI	GAWTC	2 cut(s)	261, 286
PfoI	TCCNGGA	1 cut(s)	1218
PkrI	GCNGC	2 cut(s)	316, 890
Ppu21I	YACGTR	1 cut(s)	25
PscI	ACATGT	2 cut(s)	476, 1274
PshAI	GACNNNNGTC	1 cut(s)	477
PshBI	ATTAAT	1 cut(s)	588
PsiI	TTATAA	1 cut(s)	594
Psp6I	CCWGG	2 cut(s)	1218, 1320
PspGI	CCWGG	2 cut(s)	1218, 1320
PspN4I	GGNNCC	1 cut(s)	1339
PstI	CTGCAG	1 cut(s)	1096
PstNI	CAGNNNCTG	1 cut(s)	977
PsuI	RGATCY	1 cut(s)	775
RsaI	GTAC	6 cut(s)	17, 23, 233, 1166, 1339, 1391
RsaNI	GTAC	6 cut(s)	16, 22, 232, 1165, 1338, 1390
SalI	GTCGAC	1 cut(s)	1244
SaqAI	TTAA	8 cut(s)	512, 588, 636, 825, 860, 1044, 1293, 1331
SatI	GCNGC	2 cut(s)	315, 889
Sau3AI	GATC	2 cut(s)	623, 775
ScrFI	CCNGG	2 cut(s)	1220, 1322
SduI	GDGCHC	1 cut(s)	759
SfaNI	GCATC	4 cut(s)	182, 263, 413, 1275
SfcI	CTRYAG	1 cut(s)	1092
SfuI	TTCGAA	1 cut(s)	36
SmlI	CTYRAG	4 cut(s)	111, 511, 983, 1043
SmoI	CTYRAG	4 cut(s)	111, 511, 983, 1043
SsiI	CCGC	3 cut(s)	317, 1414, 1435
SspMI	CTAG	1 cut(s)	1229
StyD4I	CCNGG	2 cut(s)	1218, 1320
TaaI	ACNGT	2 cut(s)	658, 1074
TaiI	ACGT	1 cut(s)	27
TaqI	TCGA	6 cut(s)	36, 84, 1115, 1179, 1245, 1530
TatI	WGTACW	2 cut(s)	231, 1389
TfiI	GAWTC	2 cut(s)	261, 286
Tru1I	TTAA	8 cut(s)	512, 588, 636, 825, 860, 1044, 1293, 1331
Tru9I	TTAA	8 cut(s)	512, 588, 636, 825, 860, 1044, 1293, 1331
TscAI	CASTG	1 cut(s)	978
TseFI	GTSAC	1 cut(s)	224
TseI	GCWGC	2 cut(s)	314, 888
Tsp45I	GTSAC	1 cut(s)	224
TspDTI	ATGAA	9 cut(s)	156, 176, 366, 758, 905, 933, 954, 974, 1386
TspGWI	ACGGA	2 cut(s)	68, 1417
TspRI	CASTG	1 cut(s)	978
Vha464I	CTTAAG	2 cut(s)	511, 1043
VspI	ATTAAT	1 cut(s)	588
XapI	RAATTY	5 cut(s)	154, 163, 920, 961, 1311
XceI	RCATGY	2 cut(s)	480, 1278
XmiI	GTMKAC	2 cut(s)	1245, 1512
XmnI	GAANNNNTTC	2 cut(s)	32, 341
XspI	CTAG	1 cut(s)	1229

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.