Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr6

Orthogroup ID

OG0002254

Physical Location & Seq

Genomic Coordinates Reverse (-)

40577177 .. 40580244

Gene Structure 3068 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw6G020310.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1641 bp

ATGACCGTAGTCGTCAGTGGGAGATGCAGACATCAGGGGGAGGTGTCTACATGTATGGTCTCAAAACACTCGCGATCCAAGGAAGCCTCTTCCCCCATTTATGTAGACAAGATGGCAAACAGCAAATACGAGTACGTCAAGTCCTACGAGTTTGAAGACGAGGTTTTCCTGCCCAACTTGATCGTCGTTCGCATCGACGGCCGTGATTTTCGAACGTTTTCTGAAGTGCATGAGTTTGAGAAACCAAATGATGAGGTAGCACTCAACTTGATGAATTCATGTGCAGTTTCAGTGTCGGAGGAGTTTCCGGACATAGTGTTCTCTTATGGGTTCAGTGACGAGTTCAGCTTTGTCTTCAAGAAGACGTCCAAGTTCCACCAGAGGCGTGCCAGCAAAGTAGAGTCCATTGTTGTATCCTTCTTCACCTCGGTGTATGTCTCGAAATGGAGGGAATTCTTCCCTAGTAAGGAGCTGAGATACCCTCCTTCGTTTCGCTCACGGGTTATAGCCTGCGCTACGATTGAGGTTCTTCAATCGTATCTTCTGTGGAGACAGAGTAGTTGTCATACAAGCAACCTGCACGGTACTTGCCTGTGGGAGCTTGTAAAAGATGGGAAGACGGAAAGCGAAGCACTGGAGTTACTGAAGGGAGCCAGTAAAGAAGAGAAAAACAGCCTTCTTTTTGAAAAGTTTGACATCAACTATCAAAAATTGGATGCTATATTCCGTCAAGGATCCTGCATTTTGAAAACTGAGGTGATGGACGTTGTGAAGCACAATGAGAATGGTTCTCCTGTGGAGAGGCTTAGGAAAAAGTTAAGAATACTTCATTCGAAGAATATAGCTGGGCGAAGTTTTTGGAACGAGCATGCGAGTCTTAGGAATGAGCTCGGTATATTTACAAGGGACATTGGAAAAGTTGAACCCGATTATCTCAGGTCCTTCCAGTTTGGGAACAAATTGATGCCATCAACGTGGATTGTTATTAGAATCGATGGTTGCCACTTTCACAGGTTTACTGAAGTTCATGAGTTTGTGAAGCCGAATGATGAGCAAGCTCTCAACCTTATGAATTCATGTGCCGTTGCTGTGGTGAAAGAATTTCCAGACATTATTTTCTCCTATGGCGTGAGCGATGAATACAGCTTTGTTTTCAAGAAGAATTCTCAGTTCTGTCAAAGGCAAGCAAGTGAGATGGTATCTGTCATTGTGTCATTCTTCACTTCCTCATATGTGACGAAGTGGAGTGGTTTCTTCCCTCAGAAAGAGTTGAAGTGCCCTCCTTATTTTGATGGGCGTGCTGTATGCTATCCGTCATCTGATATTCTTCGAGATTATCTGGCATGGAGGCAAGTTGACTGCCATATCAACAATCAATACAATACTTGTTTTTGGGAGCTTGTTATGTCTGGAAAAGGCAAAAGTGAAGCTCAGAATTTTCTTAAGGGTACTCAAGCAGGCGACAAAGATAGACTGCTTAAGCAATTTGGTATTGACTACAACAAGTTACAAGTTATGTTTCGCCAAGGTTCATCCATTTTCTGGGATAAGGGAGATTCTGGGGAAAAAGTTGTTGTAGAACATTGTAATATTATTGAGCCAAGTTTTTGGTACGCACACCCAACTATTCTGCATGGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000086 GO:0000166 GO:0000278 GO:0000287 GO:0000966 GO:0001882 GO:0001883 GO:0003674 GO:0003824 GO:0005488 GO:0005515 GO:0005524 GO:0005525 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005739 GO:0006139 GO:0006396 GO:0006399 GO:0006400 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0007049 GO:0008033 GO:0008144 GO:0008150 GO:0008152 GO:0008192 GO:0008193 GO:0008283 GO:0009451 GO:0009987 GO:0010467 GO:0016043 GO:0016070 GO:0016740 GO:0016772 GO:0016779 GO:0017076 GO:0019001 GO:0022402 GO:0022607 GO:0030554 GO:0031974 GO:0031981 GO:0032549 GO:0032550 GO:0032553 GO:0032555 GO:0032559 GO:0032561 GO:0033554 GO:0034470 GO:0034471 GO:0034641 GO:0034660 GO:0035639 GO:0036094 GO:0042802 GO:0043167 GO:0043168 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043412 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0044770 GO:0044772 GO:0044839 GO:0046483 GO:0046872 GO:0050896 GO:0051259 GO:0051260 GO:0051262 GO:0051289 GO:0051716 GO:0065003 GO:0070013 GO:0070568 GO:0071704 GO:0071840 GO:0090304 GO:0097159 GO:0097367 GO:0099116 GO:1901265 GO:1901360 GO:1901363 GO:1903047

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Thg1 Thg1C

Protein Analysis

546

Amino Acids

63.22

Weight (kDa)

7.83

Isoelectric Point (pI)

43.53

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Thg1	PF04446	43 - 170	4.1e-47	tRNAHis guanylyltransferase
Thg1C	PF14413	176 - 262	2.8e-24	Thg1 C terminal domain
Thg1	PF04446	310 - 438	2.2e-51	tRNAHis guanylyltransferase
Thg1C	PF14413	442 - 520	1.8e-31	Thg1 C terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0002254)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G31580 AT2G31580 AT2G31580 AT2G31580 AT2G31580 AT2G32320 AT2G32320 AT2G32320
fragaria_vesca	FvH4_1g22971 FvH4_1g22971 FvH4_1g22971 FvH4_1g22971 FvH4_1g22971 FvH4_1g22971 FvH4_1g22971 FvH4_2g15830
malus_domestica	MD05G1030700.v1.1
prunus_persica	Prupe.1G573900_v2.0.a1
pyrus_communis	pycom05g02180 pycom10g02040
rosa_chinensis	RchiOBHm_Chr2g0117491 RchiOBHm_Chr2g0117501 RchiOBHm_Chr6g0279211
rosa_laevigata	RLG00000013155 RLG00000018350
rosa_multiflora	Rmu_co8223372.1_g000001 Rmu_sc0003454.1_g000019 Rmu_sc0003454.1_g000020 Rmu_sc0014203.1_g000009 Rmu_sc0014536.1_g000004 Rmu_sc0041068.1_g000002
rosa_roxburghii	Rroxscaffold_2G00126490 Rroxscaffold_7G00189030
rosa_rugosa	Rorug02G0212800 Rorug06G0123800
rosa_samantha	Rh2CG271800 Rh2DG275200 Rh2DG294700 Rh6AG232000 Rh6BG236900 Rh6CG238800 Rh6DG230400
rosa_wichuraiana	Rw6G020310

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	368
Acc36I	ACCTGC	1 cut(s)	585
AccB7I	CCANNNNNTGG	1 cut(s)	1542
AccI	GTMKAC	2 cut(s)	47, 105
AccII	CGCG	1 cut(s)	73
AccIII	TCCGGA	1 cut(s)	307
AclI	AACGTT	1 cut(s)	215
AclWI	GGATC	3 cut(s)	69, 729, 742
AcoI	YGGCCR	1 cut(s)	199
AcsI	RAATTY	6 cut(s)	274, 452, 1072, 1100, 1162, 1435
AcuI	CTGAAG	3 cut(s)	243, 665, 1041
AcyI	GRCGYC	1 cut(s)	365
AfaI	GTAC	4 cut(s)	134, 586, 1450, 1613
AfiI	CCNNNNNNNGG	2 cut(s)	466, 1542
AflII	CTTAAG	2 cut(s)	1442, 1478
AflIII	ACRYGT	1 cut(s)	50
AgsI	TTSAA	8 cut(s)	155, 358, 533, 686, 748, 923, 1156, 1273
AleI	CACNNNNGTG	1 cut(s)	428
AloI	GAACNNNNNNTCC	2 cut(s)	302, 334
AluBI	AGCT	9 cut(s)	348, 472, 601, 845, 889, 1058, 1146, 1399, 1430
AluI	AGCT	9 cut(s)	348, 472, 601, 845, 889, 1058, 1146, 1399, 1430
Alw21I	GWGCWC	1 cut(s)	891
Alw26I	GTCTC	3 cut(s)	64, 442, 544
AlwI	GGATC	3 cut(s)	69, 729, 742
Aor13HI	TCCGGA	1 cut(s)	307
AoxI	GGCC	1 cut(s)	199
ApoI	RAATTY	6 cut(s)	274, 452, 1072, 1100, 1162, 1435
Asp700I	GAANNNNTTC	1 cut(s)	217
AspLEI	GCGC	1 cut(s)	515
AspS9I	GGNCC	1 cut(s)	939
AsuHPI	GGTGA	3 cut(s)	415, 769, 1105
AsuII	TTCGAA	2 cut(s)	211, 833
AvaII	GGWCC	1 cut(s)	939
BaeGI	GKGCMC	1 cut(s)	1280
BamHI	GGATCC	1 cut(s)	734
BanII	GRGCYC	1 cut(s)	891
BbsI	GAAGAC	4 cut(s)	162, 346, 368, 623
Bbv12I	GWGCWC	1 cut(s)	891
BccI	CCATC	7 cut(s)	106, 605, 754, 976, 989, 1189, 1286
BceAI	ACGGC	3 cut(s)	186, 214, 1067
BciVI	GTATCC	1 cut(s)	424
BcoDI	GTCTC	3 cut(s)	64, 442, 544
BfaI	CTAG	1 cut(s)	462
BfrI	CTTAAG	2 cut(s)	1442, 1478
BfuAI	ACCTGC	1 cut(s)	585
BfuI	GTATCC	1 cut(s)	424
Bme18I	GGWCC	1 cut(s)	939
BmgT120I	GGNCC	1 cut(s)	939
BmiI	GGNNCC	2 cut(s)	652, 736
BmsI	GCATC	4 cut(s)	14, 201, 706, 954
BoxI	GACNNNNGTC	1 cut(s)	8
BpiI	GAAGAC	4 cut(s)	162, 346, 368, 623
BplI	GAGNNNNNCTC	2 cut(s)	466, 498
BpmI	CTGGAG	1 cut(s)	656
Bpu10I	CCTNAGC	1 cut(s)	806
Bpu14I	TTCGAA	2 cut(s)	211, 833
BpuEI	CTTGAG	1 cut(s)	1437
Bsa29I	ATCGAT	1 cut(s)	993
BsaHI	GRCGYC	1 cut(s)	365
BsaI	GGTCTC	1 cut(s)	64
BsaJI	CCNNGG	3 cut(s)	78, 426, 1525
BsaWI	WCCGGW	1 cut(s)	307
BsaXI	ACNNNNNCTCC	2 cut(s)	541, 571
Bsc4I	CCNNNNNNNGG	2 cut(s)	466, 1542
Bse1I	ACTGG	3 cut(s)	639, 654, 946
BseAI	TCCGGA	1 cut(s)	307
BseCI	ATCGAT	1 cut(s)	993
BseDI	CCNNGG	3 cut(s)	78, 426, 1525
BseGI	GGATG	2 cut(s)	721, 1532
BseLI	CCNNNNNNNGG	2 cut(s)	466, 1542
BseMII	CTCAG	6 cut(s)	464, 744, 949, 1181, 1274, 1445
BseNI	ACTGG	3 cut(s)	639, 654, 946
BseRI	GAGGAG	1 cut(s)	314
BseSI	GKGCMC	1 cut(s)	1280
BseX3I	CGGCCG	1 cut(s)	199
BseYI	CCCAGC	1 cut(s)	845
BsgI	GTGCAG	2 cut(s)	303, 563
Bsh1236I	CGCG	1 cut(s)	73
Bsh1285I	CGRYCG	1 cut(s)	202
BshFI	GGCC	1 cut(s)	201
BshVI	ATCGAT	1 cut(s)	993
BsiEI	CGRYCG	1 cut(s)	202
BsiHKAI	GWGCWC	1 cut(s)	891
BsiSI	CCGG	1 cut(s)	308
BslFI	GGGAC	1 cut(s)	920
BslI	CCNNNNNNNGG	2 cut(s)	466, 1542
BsmAI	GTCTC	3 cut(s)	64, 442, 544
BsmFI	GGGAC	1 cut(s)	920
BsnI	GGCC	1 cut(s)	201
Bso31I	GGTCTC	1 cut(s)	64
Bsp119I	TTCGAA	2 cut(s)	211, 833
Bsp1286I	GDGCHC	2 cut(s)	891, 1280
Bsp13I	TCCGGA	1 cut(s)	307
Bsp143I	GATC	3 cut(s)	74, 180, 734
Bsp68I	TCGCGA	1 cut(s)	73
BspANI	GGCC	1 cut(s)	201
BspCNI	CTCAG	6 cut(s)	465, 745, 948, 1180, 1273, 1444
BspDI	ATCGAT	1 cut(s)	993
BspEI	TCCGGA	1 cut(s)	307
BspFNI	CGCG	1 cut(s)	73
BspHI	TCATGA	1 cut(s)	1027
BspLI	GGNNCC	2 cut(s)	652, 736
BspMI	ACCTGC	1 cut(s)	585
BspPI	GGATC	3 cut(s)	69, 729, 742
BspT104I	TTCGAA	2 cut(s)	211, 833
BspTI	CTTAAG	2 cut(s)	1442, 1478
BspTNI	GGTCTC	1 cut(s)	64
BsrI	ACTGG	3 cut(s)	639, 654, 946
BssECI	CCNNGG	3 cut(s)	78, 426, 1525
BssMI	GATC	3 cut(s)	74, 180, 734
BssNI	GRCGYC	1 cut(s)	365
BssT1I	CCWWGG	2 cut(s)	78, 1525
Bst4CI	ACNGT	2 cut(s)	7, 584
Bst6I	CTCTTC	2 cut(s)	94, 657
BstACI	GRCGYC	1 cut(s)	365
BstAFI	CTTAAG	2 cut(s)	1442, 1478
BstBI	TTCGAA	2 cut(s)	211, 833
BstC8I	GCNNGC	8 cut(s)	387, 391, 511, 870, 1056, 1185, 1299, 1459
BstDEI	CTNAG	8 cut(s)	473, 753, 806, 878, 935, 1167, 1260, 1431
BstF5I	GGATG	2 cut(s)	721, 1532
BstFNI	CGCG	1 cut(s)	73
BstHHI	GCGC	1 cut(s)	515
BstKTI	GATC	3 cut(s)	77, 183, 737
BstMAI	GTCTC	3 cut(s)	64, 442, 544
BstMBI	GATC	3 cut(s)	74, 180, 734
BstMCI	CGRYCG	1 cut(s)	202
BstMWI	GCNNNNNNNGC	1 cut(s)	198
BstNSI	RCATGY	2 cut(s)	54, 872
BstPAI	GACNNNNGTC	1 cut(s)	8
BstSLI	GKGCMC	1 cut(s)	1280
BstUI	CGCG	1 cut(s)	73
BstV2I	GAAGAC	4 cut(s)	162, 346, 368, 623
BstX2I	RGATCY	1 cut(s)	734
BstXI	CCANNNNNNTGG	2 cut(s)	975, 1608
BstYI	RGATCY	1 cut(s)	734
BstZI	CGGCCG	1 cut(s)	199
Bsu15I	ATCGAT	1 cut(s)	993
BsuI	GTATCC	1 cut(s)	424
BsuRI	GGCC	1 cut(s)	201
BsuTUI	ATCGAT	1 cut(s)	993
BtgZI	GCGATG	1 cut(s)	1149
BtsCI	GGATG	2 cut(s)	721, 1532
BtsIMutI	CAGTG	4 cut(s)	22, 297, 340, 632
BtuMI	TCGCGA	1 cut(s)	73
BveI	ACCTGC	1 cut(s)	585
Cac8I	GCNNGC	8 cut(s)	387, 391, 511, 870, 1056, 1185, 1299, 1459
CciI	TCATGA	1 cut(s)	1027
CfoI	GCGC	1 cut(s)	515
Cfr13I	GGNCC	1 cut(s)	939
ClaI	ATCGAT	1 cut(s)	993
Csp6I	GTAC	4 cut(s)	133, 585, 1449, 1612
CviAII	CATG	8 cut(s)	51, 230, 279, 869, 1028, 1077, 1344, 1634
CviQI	GTAC	4 cut(s)	133, 585, 1449, 1612
DdeI	CTNAG	8 cut(s)	473, 753, 806, 878, 935, 1167, 1260, 1431
DpnI	GATC	3 cut(s)	76, 182, 736
DpnII	GATC	3 cut(s)	74, 180, 734
EaeI	YGGCCR	1 cut(s)	199
EagI	CGGCCG	1 cut(s)	199
Eam1104I	CTCTTC	2 cut(s)	94, 657
EarI	CTCTTC	2 cut(s)	94, 657
Ecl136II	GAGCTC	1 cut(s)	889
EclXI	CGGCCG	1 cut(s)	199
Eco130I	CCWWGG	2 cut(s)	78, 1525
Eco24I	GRGCYC	1 cut(s)	891
Eco31I	GGTCTC	1 cut(s)	64
Eco47I	GGWCC	1 cut(s)	939
Eco52I	CGGCCG	1 cut(s)	199
Eco53kI	GAGCTC	1 cut(s)	889
Eco57I	CTGAAG	3 cut(s)	243, 665, 1041
EcoICRI	GAGCTC	1 cut(s)	889
EcoO109I	RGGNCCY	1 cut(s)	939
EcoRI	GAATTC	4 cut(s)	274, 452, 1072, 1162
EcoT14I	CCWWGG	2 cut(s)	78, 1525
EcoT38I	GRGCYC	1 cut(s)	891
ErhI	CCWWGG	2 cut(s)	78, 1525
FaeI	CATG	8 cut(s)	54, 233, 282, 872, 1031, 1080, 1347, 1637
FaqI	GGGAC	1 cut(s)	920
FatI	CATG	8 cut(s)	50, 229, 278, 868, 1027, 1076, 1343, 1633
FauNDI	CATATG	1 cut(s)	1231
FblI	GTMKAC	2 cut(s)	47, 105
FokI	GGATG	2 cut(s)	728, 1519
FriOI	GRGCYC	1 cut(s)	891
FspBI	CTAG	1 cut(s)	462
GlaI	GCGC	1 cut(s)	514
GsaI	CCCAGC	1 cut(s)	849
GsuI	CTGGAG	1 cut(s)	656
HaeIII	GGCC	1 cut(s)	201
HapII	CCGG	1 cut(s)	308
HhaI	GCGC	1 cut(s)	515
Hin1I	GRCGYC	1 cut(s)	365
Hin1II	CATG	8 cut(s)	54, 233, 282, 872, 1031, 1080, 1347, 1637
Hin6I	GCGC	1 cut(s)	513
HinP1I	GCGC	1 cut(s)	513
HincII	GTYRAC	1 cut(s)	1357
HindII	GTYRAC	1 cut(s)	1357
HinfI	GANTC	4 cut(s)	401, 874, 990, 1556
HpaII	CCGG	1 cut(s)	308
HphI	GGTGA	3 cut(s)	415, 769, 1105
Hpy166II	GTNNAC	4 cut(s)	48, 106, 1017, 1357
Hpy188I	TCNGA	5 cut(s)	223, 298, 1263, 1321, 1434
Hpy188III	TCNNGA	9 cut(s)	72, 308, 358, 439, 1028, 1106, 1156, 1331, 1410
Hpy8I	GTNNAC	4 cut(s)	48, 106, 1017, 1357
Hpy99I	CGWCG	2 cut(s)	188, 200
HpyAV	CCTTC	5 cut(s)	427, 495, 640, 686, 952
HpyCH4III	ACNGT	2 cut(s)	7, 584
HpyCH4IV	ACGT	5 cut(s)	135, 215, 365, 765, 974
HpyCH4V	TGCA	6 cut(s)	27, 229, 284, 580, 741, 1633
HpyF10VI	GCNNNNNNNGC	1 cut(s)	198
HpyF3I	CTNAG	8 cut(s)	473, 753, 806, 878, 935, 1167, 1260, 1431
HpySE526I	ACGT	5 cut(s)	135, 215, 365, 765, 974
Hsp92I	GRCGYC	1 cut(s)	365
Hsp92II	CATG	8 cut(s)	54, 233, 282, 872, 1031, 1080, 1347, 1637
HspAI	GCGC	1 cut(s)	513
Kpn2I	TCCGGA	1 cut(s)	307
Kzo9I	GATC	3 cut(s)	74, 180, 734
LmnI	GCTCC	4 cut(s)	469, 598, 650, 1396
LweI	GCATC	4 cut(s)	14, 201, 706, 954
MaeI	CTAG	1 cut(s)	462
MaeII	ACGT	5 cut(s)	135, 215, 365, 765, 974
MaeIII	GTNAC	4 cut(s)	335, 639, 1234, 1506
MalI	GATC	3 cut(s)	76, 182, 736
MboI	GATC	3 cut(s)	74, 180, 734
MflI	RGATCY	1 cut(s)	734
MhlI	GDGCHC	2 cut(s)	891, 1280
MluCI	AATT	9 cut(s)	274, 452, 710, 959, 1072, 1100, 1162, 1435, 1484
MlyI	GAGTC	2 cut(s)	410, 883
MmeI	TCCRAC	1 cut(s)	276
MroI	TCCGGA	1 cut(s)	307
MroXI	GAANNNNTTC	1 cut(s)	217
MseI	TTAA	3 cut(s)	818, 1443, 1479
MslI	CAYNNNNRTG	2 cut(s)	428, 973
MspCI	CTTAAG	2 cut(s)	1442, 1478
MspI	CCGG	1 cut(s)	308
MvnI	CGCG	1 cut(s)	73
MwoI	GCNNNNNNNGC	1 cut(s)	198
NdeI	CATATG	1 cut(s)	1231
NdeII	GATC	3 cut(s)	74, 180, 734
NlaIII	CATG	8 cut(s)	54, 233, 282, 872, 1031, 1080, 1347, 1637
NlaIV	GGNNCC	2 cut(s)	652, 736
NmuCI	GTSAC	2 cut(s)	335, 1234
NruI	TCGCGA	1 cut(s)	73
NspI	RCATGY	2 cut(s)	54, 872
NspV	TTCGAA	2 cut(s)	211, 833
OliI	CACNNNNGTG	1 cut(s)	428
PaeI	GCATGC	1 cut(s)	872
PagI	TCATGA	1 cut(s)	1027
PciI	ACATGT	1 cut(s)	50
PcsI	WCGNNNNNNNCGW	1 cut(s)	192
PdmI	GAANNNNTTC	1 cut(s)	217
PfeI	GAWTC	2 cut(s)	990, 1556
PflMI	CCANNNNNTGG	1 cut(s)	1542
PleI	GAGTC	2 cut(s)	409, 882
PpsI	GAGTC	2 cut(s)	409, 882
PpuMI	RGGWCCY	1 cut(s)	939
PscI	ACATGT	1 cut(s)	50
PshAI	GACNNNNGTC	1 cut(s)	8
Psp124BI	GAGCTC	1 cut(s)	891
Psp1406I	AACGTT	1 cut(s)	215
Psp5II	RGGWCCY	1 cut(s)	939
PspFI	CCCAGC	1 cut(s)	845
PspN4I	GGNNCC	2 cut(s)	652, 736
PspPI	GGNCC	1 cut(s)	939
PspPPI	RGGWCCY	1 cut(s)	939
PsuI	RGATCY	1 cut(s)	734
RruI	TCGCGA	1 cut(s)	73
RsaI	GTAC	4 cut(s)	134, 586, 1450, 1613
RsaNI	GTAC	4 cut(s)	133, 585, 1449, 1612
RseI	CAYNNNNRTG	2 cut(s)	428, 973
SacI	GAGCTC	1 cut(s)	891
SaqAI	TTAA	3 cut(s)	818, 1443, 1479
Sau3AI	GATC	3 cut(s)	74, 180, 734
Sau96I	GGNCC	1 cut(s)	939
SchI	GAGTC	2 cut(s)	410, 883
SduI	GDGCHC	2 cut(s)	891, 1280
SfaNI	GCATC	4 cut(s)	14, 201, 706, 954
SfuI	TTCGAA	2 cut(s)	211, 833
SinI	GGWCC	1 cut(s)	939
SmiMI	CAYNNNNRTG	2 cut(s)	428, 973
SmlI	CTYRAG	3 cut(s)	1442, 1452, 1478
SmoI	CTYRAG	3 cut(s)	1442, 1452, 1478
SphI	GCATGC	1 cut(s)	872
Sse9I	AATT	9 cut(s)	274, 452, 710, 959, 1072, 1100, 1162, 1435, 1484
SspI	AATATT	1 cut(s)	1591
SspMI	CTAG	1 cut(s)	462
SstI	GAGCTC	1 cut(s)	891
StyI	CCWWGG	2 cut(s)	78, 1525
TaaI	ACNGT	2 cut(s)	7, 584
TaiI	ACGT	5 cut(s)	138, 218, 368, 768, 977
TaqI	TCGA	6 cut(s)	195, 211, 440, 833, 993, 1330
TasI	AATT	9 cut(s)	274, 452, 710, 959, 1072, 1100, 1162, 1435, 1484
TfiI	GAWTC	2 cut(s)	990, 1556
Tru1I	TTAA	3 cut(s)	818, 1443, 1479
Tru9I	TTAA	3 cut(s)	818, 1443, 1479
TscAI	CASTG	4 cut(s)	22, 297, 340, 639
TseFI	GTSAC	2 cut(s)	335, 1234
Tsp45I	GTSAC	2 cut(s)	335, 1234
TspDTI	ATGAA	8 cut(s)	267, 287, 818, 1016, 1065, 1085, 1152, 1521
TspGWI	ACGGA	3 cut(s)	635, 716, 1302
TspRI	CASTG	4 cut(s)	22, 297, 340, 639
Van91I	CCANNNNNTGG	1 cut(s)	1542
Vha464I	CTTAAG	2 cut(s)	1442, 1478
VpaK11BI	GGWCC	1 cut(s)	939
XapI	RAATTY	6 cut(s)	274, 452, 1072, 1100, 1162, 1435
XceI	RCATGY	2 cut(s)	54, 872
XmiI	GTMKAC	2 cut(s)	47, 105
XmnI	GAANNNNTTC	1 cut(s)	217
XspI	CTAG	1 cut(s)	462
ZraI	GACGTC	1 cut(s)	366

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.