AT2G46280

component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0003764
Physical Location & Seq
Reverse (-)
19003158 .. 19005732
2575 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G46280.3

Sequence Viewer

Length: 987 bp
ATGAGGCCGATCCTGATGAAGGGACATGAACGTCCATTGACGTTTCTGAGGTACAACAGAGAAGGAGATCTGCTTTTCTCCTGCGCCAAGGACCACACACCCACCCTCTGGTTTGCCGATAACGGCGAGCGCCTTGGAACTTACCGTGGTCACAACGGTGCTGTTTGGTGCTGTGATGTCTCCCGAGACTCGTCAAGATTGATCACTGGTAGTGCTGATCAGACTGCAAAGCTGTGGGATGTAAAATCTGGAAAAGAATTGTTCACTTTCAAGTTTAATGCCCCTACGAGGTCTGTGGATTTCGCTGTTGGAGATCGTCTTGCAGTGATTACCACTGATCACTTCGTGGACCGTACCGCTGCTATTCATGTGAAGCGTATTGCGGAAGATCCTGAGGAACAGGATGCTGAGTCTGTGCTTGTCCTTCATTGTCCTGATGGAAAGAAGAGGATCAATAGAGCTGTTTGGGGTCCCCTGAACCAAACTATTGTTAGTGGTGGTGAAGATAAAGTTATCAGAATCTGGGATGCCGAGACGGGAAAATTGCTCAAGCAATCAGATGAGGAAGTGGGTCACAAGAAGGACATTACATCGCTCTGCAAAGCAGCTGATGACTCTCACTTCCTTACAGGTTCACTTGACAAAACTGCAAAGCTTTGGGACATGAGAACGCTGACTCTTCTTAAGACTTACACCACTGTGGTGCCCGTAAATGCTGTCTCCCTGTCTCCACTTCTCAACCATGTTGTACTTGGAGGTGGTCAAGATGCATCAGCTGTGACTACCACTGATCATCGTGCTGGAAAGTTTGAAGCTAAGTTTTACGACAAGATTCTGCAAGAGGAAATTGGTGGTGTGAAAGGTCATTTTGGACCTATAAATGCTTTGGCATTCAATCCTGATGGGAAGAGTTTCTCTAGTGGAGGTGAAGACGGTTACGTGAGGCTGCATCATTTTGACTCCGACTACTTCAACATCAAGATTTAG

Protein Analysis

328

Amino Acids

36.39

Weight (kDa)

6.5

Isoelectric Point (pI)

30.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EIF3I PF24805 1 - 250 8.1e-80 EIF3I
Beta-prop_WDR3_1st PF25173 5 - 90 3.8e-15 WDR3 first beta-propeller domain
Beta-prop_WDR5 PF25175 6 - 106 1.4e-16 WDR5 beta-propeller domain
WD40_CDC20-Fz PF24807 6 - 175 1.7e-11 CDC20/Fizzy WD40 domain
WD40_Prp19 PF24814 6 - 93 1.5e-08 Prp19 WD40 domain
Beta-prop_WDR3_2nd PF25172 7 - 80 1.1e-09 WDR3 second beta-propeller domain
Beta-prop_TEP1_2nd PF25047 7 - 85 1.7e-07 TEP-1 second beta-propeller
WDR55 PF24796 7 - 92 6e-06 WDR55
WD40_Gbeta PF25391 8 - 90 6.5e-08 G protein beta WD-40 repeat protein
Beta-prop_WDR36-Utp21_2nd PF25168 11 - 111 1.3e-07 WDR36/Utp21 second beta-propeller domain
WD40_WDHD1_1st PF24817 12 - 200 1.3e-12 WDHD1 first WD40 domain
Beta-prop_THOC3 PF25174 13 - 129 7.7e-17 THOC3 beta-propeller domain
WD40 PF00400 42 - 80 1.1e-10 WD domain, G-beta repeat
Beta-prop_WDR3_1st PF25173 147 - 232 3.6e-11 WDR3 first beta-propeller domain
WD40_Prp19 PF24814 147 - 232 5.4e-11 Prp19 WD40 domain
WD40_CDC20-Fz PF24807 147 - 247 5.8e-08 CDC20/Fizzy WD40 domain
Beta-prop_WDR5 PF25175 149 - 244 2.7e-13 WDR5 beta-propeller domain
Beta-prop_CAF1B_HIR1 PF24105 149 - 253 1.5e-07 CAF1B/HIR1 beta-propeller domain
Beta-prop_THOC3 PF25174 149 - 244 1.4e-14 THOC3 beta-propeller domain
Beta-prop_WDR3_2nd PF25172 158 - 228 9.7e-06 WDR3 second beta-propeller domain
WD40_Gbeta PF25391 160 - 252 3.1e-10 G protein beta WD-40 repeat protein
WDR55 PF24796 161 - 226 6.8e-07 WDR55
WD40 PF00400 191 - 221 9.5e-07 WD domain, G-beta repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 30
AccB1I GGYRCC 1 cut(s) 703
AccB7I CCANNNNNTGG 1 cut(s) 108
AciI CCGC 2 cut(s) 357, 383
AclWI GGATC 3 cut(s) 4, 383, 458
AdeI CACNNNGTG 1 cut(s) 346
AfaI GTAC 3 cut(s) 53, 355, 750
AfiI CCNNNNNNNGG 3 cut(s) 19, 108, 288
AflII CTTAAG 1 cut(s) 683
AgsI TTSAA 4 cut(s) 271, 812, 895, 973
AleI CACNNNNGTG 3 cut(s) 156, 698, 701
AluBI AGCT 6 cut(s) 232, 461, 608, 655, 776, 815
AluI AGCT 6 cut(s) 232, 461, 608, 655, 776, 815
Alw26I GTCTC 5 cut(s) 180, 184, 527, 724, 732
AlwI GGATC 3 cut(s) 4, 383, 458
AlwNI CAGNNNCTG 1 cut(s) 522
Ama87I CYCGRG 1 cut(s) 183
AoxI GGCC 1 cut(s) 5
ApeKI GCWGC 3 cut(s) 359, 605, 946
Asp700I GAANNNNTTC 1 cut(s) 911
AspLEI GCGC 2 cut(s) 86, 132
AspS9I GGNCC 4 cut(s) 91, 349, 470, 872
AsuHPI GGTGA 2 cut(s) 512, 938
AvaI CYCGRG 1 cut(s) 183
AvaII GGWCC 4 cut(s) 91, 349, 470, 872
AxyI CCTNAGG 1 cut(s) 393
BaeGI GKGCMC 1 cut(s) 708
BanI GGYRCC 1 cut(s) 703
BbsI GAAGAC 1 cut(s) 936
BbvI GCAGC 3 cut(s) 346, 617, 933
BccI CCATC 2 cut(s) 431, 896
BceAI ACGGC 1 cut(s) 139
BclI TGATCA 4 cut(s) 201, 217, 337, 790
BcoDI GTCTC 5 cut(s) 180, 184, 527, 724, 732
BfaI CTAG 1 cut(s) 918
BfoI RGCGCY 1 cut(s) 133
BfrI CTTAAG 1 cut(s) 683
BglII AGATCT 1 cut(s) 67
BisI GCNGC 3 cut(s) 360, 606, 947
BlsI GCNGC 3 cut(s) 361, 607, 948
Bme18I GGWCC 4 cut(s) 91, 349, 470, 872
BmeT110I CYCGRG 1 cut(s) 183
BmgT120I GGNCC 4 cut(s) 91, 349, 470, 872
BmiI GGNNCC 3 cut(s) 471, 472, 705
BmsI GCATC 5 cut(s) 394, 517, 757, 779, 958
BpiI GAAGAC 1 cut(s) 936
BpuEI CTTGAG 1 cut(s) 533
BsaAI YACGTR 1 cut(s) 940
BsaJI CCNNGG 3 cut(s) 87, 133, 145
Bsc4I CCNNNNNNNGG 3 cut(s) 19, 108, 288
Bse1I ACTGG 1 cut(s) 211
Bse21I CCTNAGG 1 cut(s) 393
BseDI CCNNGG 3 cut(s) 87, 133, 145
BseGI GGATG 3 cut(s) 244, 409, 532
BseLI CCNNNNNNNGG 3 cut(s) 19, 108, 288
BseMII CTCAG 3 cut(s) 38, 384, 399
BseNI ACTGG 1 cut(s) 211
BseSI GKGCMC 1 cut(s) 708
BseXI GCAGC 3 cut(s) 346, 617, 933
BshFI GGCC 1 cut(s) 7
BshNI GGYRCC 1 cut(s) 703
BsiHKCI CYCGRG 1 cut(s) 183
BslFI GGGAC 3 cut(s) 36, 456, 674
BslI CCNNNNNNNGG 3 cut(s) 19, 108, 288
BsmAI GTCTC 5 cut(s) 180, 184, 527, 724, 732
BsmBI CGTCTC 1 cut(s) 527
BsmFI GGGAC 3 cut(s) 36, 456, 674
BsmI GAATGC 1 cut(s) 890
BsnI GGCC 1 cut(s) 7
BsoBI CYCGRG 1 cut(s) 183
Bsp1286I GDGCHC 1 cut(s) 708
Bsp143I GATC 9 cut(s) 9, 67, 201, 217, 313, 337, 388, 450, 790
BspACI CCGC 2 cut(s) 357, 383
BspANI GGCC 1 cut(s) 7
BspCNI CTCAG 3 cut(s) 39, 385, 400
BspLI GGNNCC 3 cut(s) 471, 472, 705
BspPI GGATC 3 cut(s) 4, 383, 458
BspT107I GGYRCC 1 cut(s) 703
BspTI CTTAAG 1 cut(s) 683
BsrI ACTGG 1 cut(s) 211
BssECI CCNNGG 3 cut(s) 87, 133, 145
BssMI GATC 9 cut(s) 9, 67, 201, 217, 313, 337, 388, 450, 790
BssT1I CCWWGG 2 cut(s) 87, 133
Bst4CI ACNGT 5 cut(s) 146, 158, 353, 700, 935
Bst6I CTCTTC 3 cut(s) 440, 684, 902
BstAFI CTTAAG 1 cut(s) 683
BstBAI YACGTR 1 cut(s) 940
BstC8I GCNNGC 1 cut(s) 128
BstDEI CTNAG 4 cut(s) 47, 393, 408, 816
BstDSI CCRYGG 1 cut(s) 145
BstENI CCTNNNNNAGG 1 cut(s) 17
BstF5I GGATG 3 cut(s) 244, 409, 532
BstH2I RGCGCY 1 cut(s) 133
BstHHI GCGC 2 cut(s) 86, 132
BstKTI GATC 9 cut(s) 12, 70, 204, 220, 316, 340, 391, 453, 793
BstMAI GTCTC 5 cut(s) 180, 184, 527, 724, 732
BstMBI GATC 9 cut(s) 9, 67, 201, 217, 313, 337, 388, 450, 790
BstSLI GKGCMC 1 cut(s) 708
BstV1I GCAGC 3 cut(s) 346, 617, 933
BstV2I GAAGAC 1 cut(s) 936
BstX2I RGATCY 2 cut(s) 67, 388
BstYI RGATCY 2 cut(s) 67, 388
Bsu36I CCTNAGG 1 cut(s) 393
BsuRI GGCC 1 cut(s) 7
BtgI CCRYGG 1 cut(s) 145
BtgZI GCGATG 1 cut(s) 576
BtsCI GGATG 3 cut(s) 244, 409, 532
BtsI GCAGTG 1 cut(s) 330
BtsIMutI CAGTG 5 cut(s) 204, 330, 333, 696, 786
Cac8I GCNNGC 1 cut(s) 128
CaiI CAGNNNCTG 1 cut(s) 522
CfoI GCGC 2 cut(s) 86, 132
Cfr13I GGNCC 4 cut(s) 91, 349, 470, 872
Csp6I GTAC 3 cut(s) 52, 354, 749
CviAII CATG 4 cut(s) 26, 368, 664, 743
CviJI RGCY 8 cut(s) 7, 232, 461, 608, 655, 776, 815, 946
CviKI_1 RGCY 8 cut(s) 7, 232, 461, 608, 655, 776, 815, 946
CviQI GTAC 3 cut(s) 52, 354, 749
DdeI CTNAG 4 cut(s) 47, 393, 408, 816
DpnI GATC 9 cut(s) 11, 69, 203, 219, 315, 339, 390, 452, 792
DpnII GATC 9 cut(s) 9, 67, 201, 217, 313, 337, 388, 450, 790
DraIII CACNNNGTG 1 cut(s) 346
DrdI GACNNNNNNGTC 1 cut(s) 30
DseDI GACNNNNNNGTC 1 cut(s) 30
Eam1104I CTCTTC 3 cut(s) 440, 684, 902
EarI CTCTTC 3 cut(s) 440, 684, 902
Eco130I CCWWGG 2 cut(s) 87, 133
Eco47I GGWCC 4 cut(s) 91, 349, 470, 872
Eco81I CCTNAGG 1 cut(s) 393
Eco88I CYCGRG 1 cut(s) 183
EcoNI CCTNNNNNAGG 1 cut(s) 17
EcoO109I RGGNCCY 1 cut(s) 470
EcoT14I CCWWGG 2 cut(s) 87, 133
EcoT22I ATGCAT 1 cut(s) 772
ErhI CCWWGG 2 cut(s) 87, 133
Esp3I CGTCTC 1 cut(s) 527
FaeI CATG 4 cut(s) 29, 371, 667, 746
FaiI YATR 5 cut(s) 27, 369, 665, 744, 878
FaqI GGGAC 3 cut(s) 36, 456, 674
FatI CATG 4 cut(s) 25, 367, 663, 742
FbaI TGATCA 4 cut(s) 201, 217, 337, 790
Fnu4HI GCNGC 3 cut(s) 360, 606, 947
FokI GGATG 3 cut(s) 251, 416, 539
Fsp4HI GCNGC 3 cut(s) 360, 606, 947
FspBI CTAG 1 cut(s) 918
GlaI GCGC 2 cut(s) 85, 131
GluI GCNGC 3 cut(s) 360, 606, 947
HaeII RGCGCY 1 cut(s) 133
HaeIII GGCC 1 cut(s) 7
HhaI GCGC 2 cut(s) 86, 132
Hin1II CATG 4 cut(s) 29, 371, 667, 746
Hin6I GCGC 2 cut(s) 84, 130
HinP1I GCGC 2 cut(s) 84, 130
HindIII AAGCTT 1 cut(s) 653
HinfI GANTC 7 cut(s) 188, 410, 519, 614, 676, 832, 959
HphI GGTGA 2 cut(s) 512, 938
Hpy166II GTNNAC 3 cut(s) 264, 349, 635
Hpy188I TCNGA 5 cut(s) 48, 222, 518, 559, 964
Hpy188III TCNNGA 9 cut(s) 13, 183, 195, 249, 392, 434, 764, 899, 979
Hpy8I GTNNAC 3 cut(s) 264, 349, 635
HpyAV CCTTC 4 cut(s) 13, 56, 434, 574
HpyCH4III ACNGT 5 cut(s) 146, 158, 353, 700, 935
HpyCH4IV ACGT 3 cut(s) 31, 41, 939
HpyCH4V TGCA 7 cut(s) 227, 323, 600, 650, 770, 838, 949
HpyF3I CTNAG 4 cut(s) 47, 393, 408, 816
HpySE526I ACGT 3 cut(s) 31, 41, 939
Hsp92II CATG 4 cut(s) 29, 371, 667, 746
HspAI GCGC 2 cut(s) 84, 130
KflI GGGWCCC 1 cut(s) 470
Ksp22I TGATCA 4 cut(s) 201, 217, 337, 790
Kzo9I GATC 9 cut(s) 9, 67, 201, 217, 313, 337, 388, 450, 790
Lsp1109I GCAGC 3 cut(s) 346, 617, 933
LweI GCATC 5 cut(s) 394, 517, 757, 779, 958
MaeI CTAG 1 cut(s) 918
MaeII ACGT 3 cut(s) 31, 41, 939
MaeIII GTNAC 4 cut(s) 149, 572, 778, 935
MalI GATC 9 cut(s) 11, 69, 203, 219, 315, 339, 390, 452, 792
MboI GATC 9 cut(s) 9, 67, 201, 217, 313, 337, 388, 450, 790
MboII GAAGA 6 cut(s) 398, 457, 515, 671, 919, 941
MflI RGATCY 2 cut(s) 67, 388
MhlI GDGCHC 1 cut(s) 708
MluCI AATT 3 cut(s) 257, 542, 846
MlyI GAGTC 5 cut(s) 182, 419, 608, 670, 953
MmeI TCCRAC 2 cut(s) 289, 987
Mph1103I ATGCAT 1 cut(s) 772
MroXI GAANNNNTTC 1 cut(s) 911
MseI TTAA 2 cut(s) 276, 684
MslI CAYNNNNRTG 3 cut(s) 156, 698, 701
MspA1I CMGCKG 3 cut(s) 359, 608, 776
MspCI CTTAAG 1 cut(s) 683
Mva1269I GAATGC 1 cut(s) 890
NdeII GATC 9 cut(s) 9, 67, 201, 217, 313, 337, 388, 450, 790
NlaIII CATG 4 cut(s) 29, 371, 667, 746
NlaIV GGNNCC 3 cut(s) 471, 472, 705
NmeAIII GCCGAG 1 cut(s) 556
NmuCI GTSAC 3 cut(s) 149, 572, 778
NsiI ATGCAT 1 cut(s) 772
OliI CACNNNNGTG 3 cut(s) 156, 698, 701
PctI GAATGC 1 cut(s) 890
PdmI GAANNNNTTC 1 cut(s) 911
PfeI GAWTC 2 cut(s) 519, 832
PflMI CCANNNNNTGG 1 cut(s) 108
PkrI GCNGC 3 cut(s) 361, 607, 948
PleI GAGTC 5 cut(s) 182, 418, 608, 670, 953
PpsI GAGTC 5 cut(s) 182, 418, 608, 670, 953
Ppu21I YACGTR 1 cut(s) 940
PpuMI RGGWCCY 1 cut(s) 470
Psp5II RGGWCCY 1 cut(s) 470
PspN4I GGNNCC 3 cut(s) 471, 472, 705
PspPI GGNCC 4 cut(s) 91, 349, 470, 872
PspPPI RGGWCCY 1 cut(s) 470
PstNI CAGNNNCTG 1 cut(s) 522
PsuI RGATCY 2 cut(s) 67, 388
PvuII CAGCTG 2 cut(s) 608, 776
RsaI GTAC 3 cut(s) 53, 355, 750
RsaNI GTAC 3 cut(s) 52, 354, 749
RseI CAYNNNNRTG 3 cut(s) 156, 698, 701
SaqAI TTAA 2 cut(s) 276, 684
SatI GCNGC 3 cut(s) 360, 606, 947
Sau3AI GATC 9 cut(s) 9, 67, 201, 217, 313, 337, 388, 450, 790
Sau96I GGNCC 4 cut(s) 91, 349, 470, 872
SchI GAGTC 5 cut(s) 182, 419, 608, 670, 953
SduI GDGCHC 1 cut(s) 708
SfaNI GCATC 5 cut(s) 394, 517, 757, 779, 958
SinI GGWCC 4 cut(s) 91, 349, 470, 872
SmiMI CAYNNNNRTG 3 cut(s) 156, 698, 701
SmlI CTYRAG 2 cut(s) 548, 683
SmoI CTYRAG 2 cut(s) 548, 683
Sse9I AATT 3 cut(s) 257, 542, 846
SsiI CCGC 2 cut(s) 357, 383
SspMI CTAG 1 cut(s) 918
StyI CCWWGG 2 cut(s) 87, 133
TaaI ACNGT 5 cut(s) 146, 158, 353, 700, 935
TaiI ACGT 3 cut(s) 34, 44, 942
TasI AATT 3 cut(s) 257, 542, 846
TatI WGTACW 1 cut(s) 748
TfiI GAWTC 2 cut(s) 519, 832
Tru1I TTAA 2 cut(s) 276, 684
Tru9I TTAA 2 cut(s) 276, 684
TscAI CASTG 5 cut(s) 211, 330, 340, 703, 793
TseFI GTSAC 3 cut(s) 149, 572, 778
TseI GCWGC 3 cut(s) 359, 605, 946
Tsp45I GTSAC 3 cut(s) 149, 572, 778
TspDTI ATGAA 4 cut(s) 32, 42, 356, 416
TspRI CASTG 5 cut(s) 211, 330, 340, 703, 793
Van91I CCANNNNNTGG 1 cut(s) 108
Vha464I CTTAAG 1 cut(s) 683
VpaK11BI GGWCC 4 cut(s) 91, 349, 470, 872
XagI CCTNNNNNAGG 1 cut(s) 17
XcmI CCANNNNNNNNNTGG 1 cut(s) 749
XmnI GAANNNNTTC 1 cut(s) 911
XspI CTAG 1 cut(s) 918
Zsp2I ATGCAT 1 cut(s) 772
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.