Rroxscaffold_5G00386060

component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0003764
Physical Location & Seq
Forward (+)
65022899 .. 65029068
6170 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00386060.1

Sequence Viewer

Length: 1074 bp
ATGAGGCCGATTCTGATGAAGGGGCACGAGAGGCCCTTGACCTTCCTCAAGTACAACCGCGACGGCGACCTACTCTTCTCCTGCGCCAAGGATCACACCCCCAACGTATGGTTCGCCGACAACGGCGAGCGCCTCGGCACCTACCGCGGCCACAACGGCGCCGTTTGGTGCTGCGACGTCTCCAGAGACTCCTCCCGCCTCATCACCGGTAGCGCAGATCAGAGCGCCAAGCTTTGGGATGTACAGTCCGGCCAGCAGGTCTTCACCTTCAACTTCGACTCTCCGACGAGGTCGGTGGAGTTCTCCGTCGGCGATAAGCTCGCCGTCATCACCACCGACCCGTTCATGGAGCTCACTTCTGCGATTCACGTCAAACGCATCGCCGGAGACCCTGCCGATCGTAAGCATTTCAGCTCTTTGAACTTTGCTTTCTTCGAATTTGTGTTTAATTTGAATATGAATAATCGTTTTGGGTTTCGATTTCCAGAGACTGCCGAATCGATTCTGACACTCAAGGTTCCTCAGGGGAGAATGAAGATAAACAGAGCTGTTTGGGGGCCGTTGAACAGCACTATCATAAGCGCCGGAGAAGATGCTGTCATTCGTATCTGGGACTCTGAGACTGGAAAACTACTTCAAGAGAACCAGAAGGAAGAGGGTCACACGAAAACAATTACTTCACTAGTAAAATCTCCGGATGGTTCACACTTTCTAACCGGTTCCCTTGATAAATCTGCGAAGCTTTGGGATACTAGAACATTGACTCTTATCAAGACGTACCGGACTGAGAGTCCAGTCAATGCAGTTGCAATGTCGCCTCTTCTCAATCATGTTGTACTTGGTGGTGGTCAGGATGCAATCAATGTCACCACAACTGATCATCGTGCTGGGAAATTCGAAGCCAAATTCTATGACAAGATTCTTCAAGAAGAGATTGGAGGTGTTAAAGGGCATTTTGGACCTATTAATGCTTTGGCTTTCAACCCTGATGGTAAAAGTTTCTCAAGTGGAGGTGAAGATGGTTACATACGTCTACATCACTTTGACCATGATTACTTCAACATCAAGATTTAG

Protein Analysis

357

Amino Acids

39.89

Weight (kDa)

6.9

Isoelectric Point (pI)

35.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EIF3I PF24805 1 - 131 2.5e-53 EIF3I
Beta-prop_WDR3_1st PF25173 5 - 90 1.2e-15 WDR3 first beta-propeller domain
WD40_CDC20-Fz PF24807 6 - 106 4.7e-09 CDC20/Fizzy WD40 domain
Beta-prop_WDR5 PF25175 6 - 107 1.1e-16 WDR5 beta-propeller domain
WD40_Prp19 PF24814 6 - 93 1e-08 Prp19 WD40 domain
Beta-prop_WDR3_2nd PF25172 7 - 80 5.6e-10 WDR3 second beta-propeller domain
WDR55 PF24796 7 - 91 8.7e-06 WDR55
Beta-prop_EML_2 PF23414 9 - 87 6.8e-07 Echinoderm microtubule-associated protein second beta-propeller
WD40_Gbeta PF25391 9 - 90 5e-08 G protein beta WD-40 repeat protein
Beta-prop_WDR36-Utp21_2nd PF25168 11 - 108 1.9e-08 WDR36/Utp21 second beta-propeller domain
WD40_WDHD1_1st PF24817 12 - 93 1.1e-06 WDHD1 first WD40 domain
Beta-prop_THOC3 PF25174 13 - 123 8.9e-18 THOC3 beta-propeller domain
WD40 PF00400 42 - 80 5.3e-10 WD domain, G-beta repeat
Beta-prop_TEP1_2nd PF25047 47 - 85 7.7e-07 TEP-1 second beta-propeller
EIF3I PF24805 169 - 356 2.9e-69 EIF3I
Beta-prop_WDR3_1st PF25173 176 - 288 1.4e-10 WDR3 first beta-propeller domain
WD40_Prp19 PF24814 178 - 346 3e-15 Prp19 WD40 domain
Beta-prop_WDR5 PF25175 178 - 276 6.8e-14 WDR5 beta-propeller domain
WD40_CDC20-Fz PF24807 178 - 288 5.2e-10 CDC20/Fizzy WD40 domain
Beta-prop_THOC3 PF25174 179 - 301 8.3e-18 THOC3 beta-propeller domain
WD40_WDHD1_1st PF24817 188 - 349 2.7e-10 WDHD1 first WD40 domain
WDR55 PF24796 191 - 302 1.6e-06 WDR55
WD40_Gbeta PF25391 191 - 297 4.4e-12 G protein beta WD-40 repeat protein
Beta-prop_EML PF23409 197 - 346 9e-06 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_WDR36-Utp21_2nd PF25168 219 - 275 4.8e-07 WDR36/Utp21 second beta-propeller domain
WD40 PF00400 219 - 250 2.4e-08 WD domain, G-beta repeat
Beta-prop_EML_2 PF23414 227 - 354 1.6e-10 Echinoderm microtubule-associated protein second beta-propeller
WD40 PF00400 314 - 345 1e-06 WD domain, G-beta repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 180
Acc36I ACCTGC 1 cut(s) 247
AccB1I GGYRCC 2 cut(s) 137, 158
AccB7I CCANNNNNTGG 2 cut(s) 108, 234
AccI GTMKAC 1 cut(s) 1033
AccII CGCG 2 cut(s) 60, 147
AccIII TCCGGA 1 cut(s) 694
AciI CCGC 4 cut(s) 58, 145, 147, 196
AclWI GGATC 1 cut(s) 99
AcoI YGGCCR 2 cut(s) 148, 250
AcsI RAATTY 3 cut(s) 437, 893, 905
AcyI GRCGYC 2 cut(s) 159, 177
AfaI GTAC 4 cut(s) 53, 243, 779, 837
AfiI CCNNNNNNNGG 3 cut(s) 108, 234, 346
AgeI ACCGGT 2 cut(s) 206, 716
AgsI TTSAA 8 cut(s) 271, 421, 454, 565, 638, 926, 982, 1060
AhdI GACNNNNNGTC 1 cut(s) 789
AhlI ACTAGT 1 cut(s) 682
AjiI CACGTC 1 cut(s) 370
AjuI GAANNNNNNNTTGG 2 cut(s) 95, 127
AluBI AGCT 6 cut(s) 232, 319, 352, 414, 548, 742
AluI AGCT 6 cut(s) 232, 319, 352, 414, 548, 742
Alw21I GWGCWC 1 cut(s) 354
Alw26I GTCTC 5 cut(s) 180, 184, 381, 482, 614
AlwI GGATC 1 cut(s) 99
AlwNI CAGNNNCTG 1 cut(s) 491
Aor13HI TCCGGA 1 cut(s) 694
AoxI GGCC 5 cut(s) 5, 32, 148, 250, 557
ApeKI GCWGC 1 cut(s) 171
ApoI RAATTY 3 cut(s) 437, 893, 905
AseI ATTAAT 1 cut(s) 966
AsiGI ACCGGT 2 cut(s) 206, 716
Asp700I GAANNNNTTC 1 cut(s) 501
AspLEI GCGC 6 cut(s) 86, 132, 161, 215, 227, 584
AspS9I GGNCC 3 cut(s) 33, 557, 959
AsuHPI GGTGA 5 cut(s) 196, 256, 322, 859, 1025
AsuII TTCGAA 2 cut(s) 435, 897
AvaII GGWCC 1 cut(s) 959
AxyI CCTNAGG 1 cut(s) 522
BaeGI GKGCMC 1 cut(s) 27
BanI GGYRCC 2 cut(s) 137, 158
BanII GRGCYC 1 cut(s) 354
BauI CACGAG 1 cut(s) 26
BbsI GAAGAC 1 cut(s) 253
Bbv12I GWGCWC 1 cut(s) 354
BbvI GCAGC 1 cut(s) 158
BccI CCATC 3 cut(s) 692, 983, 1013
BceAI ACGGC 6 cut(s) 79, 139, 146, 172, 308, 544
BciVI GTATCC 1 cut(s) 742
BclI TGATCA 1 cut(s) 877
BcoDI GTCTC 5 cut(s) 180, 184, 381, 482, 614
BcuI ACTAGT 1 cut(s) 682
BfaI CTAG 2 cut(s) 683, 753
BfoI RGCGCY 4 cut(s) 133, 162, 228, 585
BfuAI ACCTGC 1 cut(s) 247
BfuI GTATCC 1 cut(s) 742
BglI GCCNNNNNGGC 1 cut(s) 156
BisI GCNGC 2 cut(s) 148, 172
BlsI GCNGC 2 cut(s) 149, 173
Bme18I GGWCC 1 cut(s) 959
BmeRI GACNNNNNGTC 1 cut(s) 789
BmgBI CACGTC 1 cut(s) 370
BmgT120I GGNCC 3 cut(s) 33, 557, 959
BmiI GGNNCC 5 cut(s) 139, 160, 519, 558, 721
BmsI GCATC 3 cut(s) 387, 583, 844
BpiI GAAGAC 1 cut(s) 253
BpmI CTGGAG 1 cut(s) 166
Bpu14I TTCGAA 2 cut(s) 435, 897
BpuEI CTTGAG 3 cut(s) 32, 497, 988
Bsa29I ATCGAT 1 cut(s) 500
BsaHI GRCGYC 2 cut(s) 159, 177
BsaI GGTCTC 1 cut(s) 381
BsaJI CCNNGG 3 cut(s) 87, 133, 145
BsaWI WCCGGW 4 cut(s) 206, 694, 716, 780
Bsc4I CCNNNNNNNGG 3 cut(s) 108, 234, 346
Bse118I RCCGGY 2 cut(s) 206, 716
Bse1I ACTGG 2 cut(s) 628, 794
Bse21I CCTNAGG 1 cut(s) 522
Bse3DI GCAATG 1 cut(s) 816
BseAI TCCGGA 1 cut(s) 694
BseCI ATCGAT 1 cut(s) 500
BseDI CCNNGG 3 cut(s) 87, 133, 145
BseGI GGATG 3 cut(s) 244, 703, 859
BseLI CCNNNNNNNGG 3 cut(s) 108, 234, 346
BseMI GCAATG 1 cut(s) 816
BseMII CTCAG 3 cut(s) 536, 609, 777
BseNI ACTGG 2 cut(s) 628, 794
BseRI GAGGAG 1 cut(s) 181
BseSI GKGCMC 1 cut(s) 27
BseXI GCAGC 1 cut(s) 158
BseYI CCCAGC 1 cut(s) 887
Bsh1236I CGCG 2 cut(s) 60, 147
Bsh1285I CGRYCG 1 cut(s) 400
BshFI GGCC 5 cut(s) 7, 34, 150, 252, 559
BshNI GGYRCC 2 cut(s) 137, 158
BshTI ACCGGT 2 cut(s) 206, 716
BshVI ATCGAT 1 cut(s) 500
BsiEI CGRYCG 1 cut(s) 400
BsiHKAI GWGCWC 1 cut(s) 354
BsiSI CCGG 7 cut(s) 207, 249, 384, 585, 695, 717, 781
BslFI GGGAC 1 cut(s) 626
BslI CCNNNNNNNGG 3 cut(s) 108, 234, 346
BsmAI GTCTC 5 cut(s) 180, 184, 381, 482, 614
BsmBI CGTCTC 1 cut(s) 184
BsmFI GGGAC 1 cut(s) 626
BsnI GGCC 5 cut(s) 7, 34, 150, 252, 559
Bso31I GGTCTC 1 cut(s) 381
Bsp119I TTCGAA 2 cut(s) 435, 897
Bsp1286I GDGCHC 2 cut(s) 27, 354
Bsp13I TCCGGA 1 cut(s) 694
Bsp1407I TGTACA 1 cut(s) 241
Bsp143I GATC 4 cut(s) 91, 217, 397, 877
BspACI CCGC 4 cut(s) 58, 145, 147, 196
BspANI GGCC 5 cut(s) 7, 34, 150, 252, 559
BspCNI CTCAG 3 cut(s) 535, 610, 778
BspDI ATCGAT 1 cut(s) 500
BspEI TCCGGA 1 cut(s) 694
BspFNI CGCG 2 cut(s) 60, 147
BspLI GGNNCC 5 cut(s) 139, 160, 519, 558, 721
BspMI ACCTGC 1 cut(s) 247
BspPI GGATC 1 cut(s) 99
BspT104I TTCGAA 2 cut(s) 435, 897
BspT107I GGYRCC 2 cut(s) 137, 158
BspTNI GGTCTC 1 cut(s) 381
BsrDI GCAATG 1 cut(s) 816
BsrFI RCCGGY 2 cut(s) 206, 716
BsrGI TGTACA 1 cut(s) 241
BsrI ACTGG 2 cut(s) 628, 794
BssAI RCCGGY 2 cut(s) 206, 716
BssECI CCNNGG 3 cut(s) 87, 133, 145
BssMI GATC 4 cut(s) 91, 217, 397, 877
BssNI GRCGYC 2 cut(s) 159, 177
BssSI CACGAG 1 cut(s) 26
BssT1I CCWWGG 1 cut(s) 87
Bst2BI CACGAG 1 cut(s) 26
Bst4CI ACNGT 1 cut(s) 246
Bst6I CTCTTC 4 cut(s) 80, 648, 825, 924
BstACI GRCGYC 2 cut(s) 159, 177
BstAUI TGTACA 1 cut(s) 241
BstBI TTCGAA 2 cut(s) 435, 897
BstC8I GCNNGC 3 cut(s) 128, 254, 321
BstDEI CTNAG 3 cut(s) 522, 618, 786
BstDSI CCRYGG 1 cut(s) 145
BstF5I GGATG 3 cut(s) 244, 703, 859
BstFNI CGCG 2 cut(s) 60, 147
BstH2I RGCGCY 4 cut(s) 133, 162, 228, 585
BstHHI GCGC 6 cut(s) 86, 132, 161, 215, 227, 584
BstKTI GATC 4 cut(s) 94, 220, 400, 880
BstMAI GTCTC 5 cut(s) 180, 184, 381, 482, 614
BstMBI GATC 4 cut(s) 91, 217, 397, 877
BstMCI CGRYCG 1 cut(s) 400
BstMWI GCNNNNNNNGC 3 cut(s) 31, 144, 156
BstSLI GKGCMC 1 cut(s) 27
BstUI CGCG 2 cut(s) 60, 147
BstV1I GCAGC 1 cut(s) 158
BstV2I GAAGAC 1 cut(s) 253
Bsu15I ATCGAT 1 cut(s) 500
Bsu36I CCTNAGG 1 cut(s) 522
BsuI GTATCC 1 cut(s) 742
BsuRI GGCC 5 cut(s) 7, 34, 150, 252, 559
BsuTUI ATCGAT 1 cut(s) 500
BtgI CCRYGG 1 cut(s) 145
BtgZI GCGATG 1 cut(s) 364
BtrI CACGTC 1 cut(s) 370
BtsCI GGATG 3 cut(s) 244, 703, 859
BveI ACCTGC 1 cut(s) 247
Cac8I GCNNGC 3 cut(s) 128, 254, 321
CaiI CAGNNNCTG 1 cut(s) 491
CfoI GCGC 6 cut(s) 86, 132, 161, 215, 227, 584
Cfr10I RCCGGY 2 cut(s) 206, 716
Cfr13I GGNCC 3 cut(s) 33, 557, 959
Cfr42I CCGCGG 1 cut(s) 148
ClaI ATCGAT 1 cut(s) 500
Csp6I GTAC 4 cut(s) 52, 242, 778, 836
CspAI ACCGGT 2 cut(s) 206, 716
CviAII CATG 3 cut(s) 346, 830, 1049
CviQI GTAC 4 cut(s) 52, 242, 778, 836
DdeI CTNAG 3 cut(s) 522, 618, 786
DinI GGCGCC 1 cut(s) 160
DpnI GATC 4 cut(s) 93, 219, 399, 879
DpnII GATC 4 cut(s) 91, 217, 397, 877
DriI GACNNNNNGTC 1 cut(s) 789
EaeI YGGCCR 2 cut(s) 148, 250
Eam1104I CTCTTC 4 cut(s) 80, 648, 825, 924
Eam1105I GACNNNNNGTC 1 cut(s) 789
EarI CTCTTC 4 cut(s) 80, 648, 825, 924
Ecl136II GAGCTC 1 cut(s) 352
Eco130I CCWWGG 1 cut(s) 87
Eco24I GRGCYC 1 cut(s) 354
Eco31I GGTCTC 1 cut(s) 381
Eco47I GGWCC 1 cut(s) 959
Eco53kI GAGCTC 1 cut(s) 352
Eco81I CCTNAGG 1 cut(s) 522
EcoICRI GAGCTC 1 cut(s) 352
EcoO109I RGGNCCY 1 cut(s) 33
EcoT14I CCWWGG 1 cut(s) 87
EcoT38I GRGCYC 1 cut(s) 354
EgeI GGCGCC 1 cut(s) 160
EheI GGCGCC 1 cut(s) 160
ErhI CCWWGG 1 cut(s) 87
Esp3I CGTCTC 1 cut(s) 184
FaeI CATG 3 cut(s) 349, 833, 1052
FaiI YATR 8 cut(s) 109, 347, 458, 578, 831, 912, 1028, 1050
FaqI GGGAC 1 cut(s) 626
FatI CATG 3 cut(s) 345, 829, 1048
FauI CCCGC 1 cut(s) 203
FbaI TGATCA 1 cut(s) 877
FblI GTMKAC 1 cut(s) 1033
Fnu4HI GCNGC 2 cut(s) 148, 172
FokI GGATG 3 cut(s) 251, 710, 866
FriOI GRGCYC 1 cut(s) 354
Fsp4HI GCNGC 2 cut(s) 148, 172
FspBI CTAG 2 cut(s) 683, 753
GlaI GCGC 6 cut(s) 85, 131, 160, 214, 226, 583
GluI GCNGC 2 cut(s) 148, 172
GsaI CCCAGC 1 cut(s) 891
GsuI CTGGAG 1 cut(s) 166
HaeII RGCGCY 4 cut(s) 133, 162, 228, 585
HaeIII GGCC 5 cut(s) 7, 34, 150, 252, 559
HapII CCGG 7 cut(s) 207, 249, 384, 585, 695, 717, 781
HhaI GCGC 6 cut(s) 86, 132, 161, 215, 227, 584
Hin1I GRCGYC 2 cut(s) 159, 177
Hin1II CATG 3 cut(s) 349, 833, 1052
Hin6I GCGC 6 cut(s) 84, 130, 159, 213, 225, 582
HinP1I GCGC 6 cut(s) 84, 130, 159, 213, 225, 582
HindIII AAGCTT 2 cut(s) 230, 740
HpaII CCGG 7 cut(s) 207, 249, 384, 585, 695, 717, 781
HphI GGTGA 5 cut(s) 196, 256, 322, 859, 1025
Hpy166II GTNNAC 2 cut(s) 704, 1034
Hpy188I TCNGA 5 cut(s) 15, 222, 285, 507, 619
Hpy188III TCNNGA 8 cut(s) 183, 485, 638, 695, 772, 851, 926, 1066
Hpy8I GTNNAC 2 cut(s) 704, 1034
Hpy99I CGWCG 4 cut(s) 65, 179, 289, 311
HpyAV CCTTC 4 cut(s) 13, 52, 277, 643
HpyCH4III ACNGT 1 cut(s) 246
HpyCH4IV ACGT 5 cut(s) 105, 177, 369, 776, 1030
HpyCH4V TGCA 3 cut(s) 803, 809, 857
HpyF10VI GCNNNNNNNGC 3 cut(s) 31, 144, 156
HpyF3I CTNAG 3 cut(s) 522, 618, 786
HpySE526I ACGT 5 cut(s) 105, 177, 369, 776, 1030
Hsp92I GRCGYC 2 cut(s) 159, 177
Hsp92II CATG 3 cut(s) 349, 833, 1052
HspAI GCGC 6 cut(s) 84, 130, 159, 213, 225, 582
KasI GGCGCC 1 cut(s) 158
Kpn2I TCCGGA 1 cut(s) 694
Ksp22I TGATCA 1 cut(s) 877
KspI CCGCGG 1 cut(s) 148
Kzo9I GATC 4 cut(s) 91, 217, 397, 877
LmnI GCTCC 1 cut(s) 349
Lsp1109I GCAGC 1 cut(s) 158
LweI GCATC 3 cut(s) 387, 583, 844
MaeI CTAG 2 cut(s) 683, 753
MaeII ACGT 5 cut(s) 105, 177, 369, 776, 1030
MaeIII GTNAC 3 cut(s) 659, 865, 1022
MalI GATC 4 cut(s) 93, 219, 399, 879
MboI GATC 4 cut(s) 91, 217, 397, 877
MhlI GDGCHC 2 cut(s) 27, 354
MluCI AATT 5 cut(s) 437, 448, 672, 893, 905
Mly113I GGCGCC 1 cut(s) 159
MlyI GAGTC 5 cut(s) 182, 272, 608, 757, 799
MmeI TCCRAC 1 cut(s) 308
MroI TCCGGA 1 cut(s) 694
MroXI GAANNNNTTC 1 cut(s) 501
MseI TTAA 3 cut(s) 447, 945, 966
MspA1I CMGCKG 1 cut(s) 147
MspI CCGG 7 cut(s) 207, 249, 384, 585, 695, 717, 781
MvnI CGCG 2 cut(s) 60, 147
MwoI GCNNNNNNNGC 3 cut(s) 31, 144, 156
NarI GGCGCC 1 cut(s) 159
NdeII GATC 4 cut(s) 91, 217, 397, 877
NlaIII CATG 3 cut(s) 349, 833, 1052
NlaIV GGNNCC 5 cut(s) 139, 160, 519, 558, 721
NmeAIII GCCGAG 1 cut(s) 114
NmuCI GTSAC 2 cut(s) 659, 865
NspV TTCGAA 2 cut(s) 435, 897
PdmI GAANNNNTTC 1 cut(s) 501
PfeI GAWTC 5 cut(s) 10, 364, 497, 502, 919
PflFI GACNNNGTC 1 cut(s) 289
PflMI CCANNNNNTGG 2 cut(s) 108, 234
PinAI ACCGGT 2 cut(s) 206, 716
PkrI GCNGC 2 cut(s) 149, 173
Ple19I CGATCG 1 cut(s) 400
PleI GAGTC 5 cut(s) 182, 272, 608, 757, 798
PluTI GGCGCC 1 cut(s) 162
PpsI GAGTC 5 cut(s) 182, 272, 608, 757, 798
PshBI ATTAAT 1 cut(s) 966
Psp124BI GAGCTC 1 cut(s) 354
PspFI CCCAGC 1 cut(s) 887
PspN4I GGNNCC 5 cut(s) 139, 160, 519, 558, 721
PspPI GGNCC 3 cut(s) 33, 557, 959
PstNI CAGNNNCTG 1 cut(s) 491
PsyI GACNNNGTC 1 cut(s) 289
PvuI CGATCG 1 cut(s) 400
RsaI GTAC 4 cut(s) 53, 243, 779, 837
RsaNI GTAC 4 cut(s) 52, 242, 778, 836
SacI GAGCTC 1 cut(s) 354
SacII CCGCGG 1 cut(s) 148
SaqAI TTAA 3 cut(s) 447, 945, 966
SatI GCNGC 2 cut(s) 148, 172
Sau3AI GATC 4 cut(s) 91, 217, 397, 877
Sau96I GGNCC 3 cut(s) 33, 557, 959
SchI GAGTC 5 cut(s) 182, 272, 608, 757, 799
SduI GDGCHC 2 cut(s) 27, 354
SfaNI GCATC 3 cut(s) 387, 583, 844
SfoI GGCGCC 1 cut(s) 160
Sfr303I CCGCGG 1 cut(s) 148
SfuI TTCGAA 2 cut(s) 435, 897
SgrBI CCGCGG 1 cut(s) 148
SinI GGWCC 1 cut(s) 959
SmlI CTYRAG 3 cut(s) 47, 512, 1003
SmoI CTYRAG 3 cut(s) 47, 512, 1003
SpeI ACTAGT 1 cut(s) 682
Sse9I AATT 5 cut(s) 437, 448, 672, 893, 905
SsiI CCGC 4 cut(s) 58, 145, 147, 196
SspDI GGCGCC 1 cut(s) 158
SspMI CTAG 2 cut(s) 683, 753
SstI GAGCTC 1 cut(s) 354
StyI CCWWGG 1 cut(s) 87
TaaI ACNGT 1 cut(s) 246
TaiI ACGT 5 cut(s) 108, 180, 372, 779, 1033
TaqI TCGA 5 cut(s) 276, 435, 478, 500, 897
TasI AATT 5 cut(s) 437, 448, 672, 893, 905
TatI WGTACW 3 cut(s) 51, 241, 835
TauI GCSGC 1 cut(s) 150
TfiI GAWTC 5 cut(s) 10, 364, 497, 502, 919
Tru1I TTAA 3 cut(s) 447, 945, 966
Tru9I TTAA 3 cut(s) 447, 945, 966
TseFI GTSAC 2 cut(s) 659, 865
TseI GCWGC 1 cut(s) 171
Tsp45I GTSAC 2 cut(s) 659, 865
TspDTI ATGAA 4 cut(s) 32, 334, 473, 548
TspGWI ACGGA 1 cut(s) 295
Tth111I GACNNNGTC 1 cut(s) 289
Van91I CCANNNNNTGG 2 cut(s) 108, 234
VpaK11BI GGWCC 1 cut(s) 959
VspI ATTAAT 1 cut(s) 966
XapI RAATTY 3 cut(s) 437, 893, 905
XmiI GTMKAC 1 cut(s) 1033
XmnI GAANNNNTTC 1 cut(s) 501
XspI CTAG 2 cut(s) 683, 753
ZraI GACGTC 1 cut(s) 178
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.