MD13G1013500.v1.1

component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0003764
Physical Location & Seq
Reverse (-)
845712 .. 848179
2468 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1013500.v1.1.491

Sequence Viewer

Length: 981 bp
ATGAGGCCGATACTGATGAAAGGGCACGAGAGGCCCCTGACTTTCCTCAAATACAACAGGGACGGCGACCTCCTCTTCTCCTGCGCCAAGGACCACAACCCCACCGTCTGGTTTGCCGACAACGGCGAGCGCCTCGGGACTTACCGCGGCCACAACGGCGCCGTTTGGTGCTGCGATGTGTCCAGAGACTCCGCCCGCCTCATTACCGGCAGCGCCGACCAGACCGCCAAGCTGTGGAACGTTCAGACCGGTCAGCAGCTTTTCACCTTCAATTTTGACTCGCCGGCGAGGGCTGTCGACTTCTCCGTCGGCGATAAGCTCGCCGTCATCACCACCGACCCTTTCATGGACGTTACGTCGGCGATTCACGTCAAGCGTATCGCCGGAGATCCCGAAGATCAGATTGGTGAATCGGCTCTGATACTCAAGGGACCTCAGGGAAGAATTAACAGAGCTGTTTGGGGTCCTCTGAACAGAACCATCATAAGCGCCGGAGAAGACGCAGTTATTCGCATTTGGGATTCTGAGACTGGTAAACTACTTAAGGAGAATGATCCGGAAGTGGGTCACAAAAAGACCATAACTTCGCTTGCGAAATCTATGGATGGTTCACATTTCCTCACCGGTTCGCTTGATAAGTCTGCCAAGCTGTGGGATACTAGAACGTTGACTCTTATCAAGACCTACGTGACCGAGCGTCCAGTCAATGCCGTTGCAATGTCTCCTCTTTGTGATCATGTGGTACTTGGTGGTGGTCAGGATGCATCAACTGTCACCACAACTGATCATCGTTCTGGGAAATTTGAAGCCAAATTCTATGACAAGATTCTTCAAGAAGAAATTGGAGGTGTTAAAGGGCATTTTGGACCTATAAATGCTTTGGCGTTTAATCCTGATGGAAAAAGTTTCTCAAGCGGGGGTGAGGATGGTTACGTTCGTCTACATCACTTTGATTCTGATTACTTCCACATCAAGATTTAG

Protein Analysis

327

Amino Acids

35.91

Weight (kDa)

6.46

Isoelectric Point (pI)

32.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EIF3I PF24805 1 - 325 6e-128 EIF3I
Beta-prop_WDR3_1st PF25173 5 - 90 1.1e-15 WDR3 first beta-propeller domain
Beta-prop_WDR5 PF25175 6 - 107 3.9e-16 WDR5 beta-propeller domain
WD40_CDC20-Fz PF24807 6 - 173 2.7e-12 CDC20/Fizzy WD40 domain
WD40_Prp19 PF24814 6 - 92 1.2e-07 Prp19 WD40 domain
Beta-prop_WDR3_2nd PF25172 7 - 80 9.9e-10 WDR3 second beta-propeller domain
Beta-prop_WDR36-Utp21_2nd PF25168 11 - 109 8.5e-07 WDR36/Utp21 second beta-propeller domain
WD40_WDHD1_1st PF24817 12 - 91 5.6e-06 WDHD1 first WD40 domain
Beta-prop_THOC3 PF25174 13 - 121 1.5e-17 THOC3 beta-propeller domain
WD40_Gbeta PF25391 14 - 80 2.7e-07 G protein beta WD-40 repeat protein
WD40 PF00400 42 - 80 8e-10 WD domain, G-beta repeat
Beta-prop_EML PF23409 48 - 265 8.1e-07 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_WDR3_2nd PF25172 50 - 175 2.2e-08 WDR3 second beta-propeller domain
Beta-prop_WDR3_1st PF25173 136 - 252 5.5e-11 WDR3 first beta-propeller domain
Beta-prop_WDR5 PF25175 141 - 265 1.1e-14 WDR5 beta-propeller domain
WD40_Prp19 PF24814 142 - 315 5.1e-16 Prp19 WD40 domain
WD40_CDC20-Fz PF24807 145 - 267 1.1e-08 CDC20/Fizzy WD40 domain
Beta-prop_THOC3 PF25174 149 - 271 2e-16 THOC3 beta-propeller domain
WD40_WDHD1_1st PF24817 158 - 319 1.3e-08 WDHD1 first WD40 domain
WD40_Gbeta PF25391 160 - 264 1.2e-10 G protein beta WD-40 repeat protein
Beta-prop_IFT122_1st PF23381 161 - 248 3.8e-06 IFT122 first beta-propeller
Beta-prop_WDR36-Utp21_2nd PF25168 189 - 247 4.1e-06 WDR36/Utp21 second beta-propeller domain
WD40 PF00400 189 - 219 9.2e-07 WD domain, G-beta repeat
Beta-prop_EML_2 PF23414 196 - 323 2.9e-09 Echinoderm microtubule-associated protein second beta-propeller
WD40 PF00400 283 - 314 9.5e-07 WD domain, G-beta repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 305
AccB1I GGYRCC 1 cut(s) 158
AccB7I CCANNNNNTGG 3 cut(s) 108, 234, 651
AccI GTMKAC 2 cut(s) 297, 940
AccII CGCG 1 cut(s) 147
AccIII TCCGGA 1 cut(s) 556
AciI CCGC 6 cut(s) 145, 147, 192, 196, 225, 915
AclI AACGTT 2 cut(s) 240, 665
AclWI GGATC 2 cut(s) 383, 548
AcoI YGGCCR 1 cut(s) 148
AcsI RAATTY 2 cut(s) 800, 812
AcyI GRCGYC 1 cut(s) 159
AfaI GTAC 1 cut(s) 744
AfiI CCNNNNNNNGG 5 cut(s) 108, 234, 346, 563, 651
AflII CTTAAG 1 cut(s) 542
AgeI ACCGGT 2 cut(s) 248, 623
AgsI TTSAA 3 cut(s) 271, 806, 833
AhdI GACNNNNNGTC 2 cut(s) 355, 696
AjiI CACGTC 1 cut(s) 370
AjuI GAANNNNNNNTTGG 2 cut(s) 387, 419
AluBI AGCT 5 cut(s) 232, 259, 319, 455, 649
AluI AGCT 5 cut(s) 232, 259, 319, 455, 649
Alw26I GTCTC 3 cut(s) 180, 521, 726
AlwI GGATC 2 cut(s) 383, 548
Ama87I CYCGRG 1 cut(s) 134
Aor13HI TCCGGA 1 cut(s) 556
AoxI GGCC 3 cut(s) 5, 32, 148
ApeKI GCWGC 3 cut(s) 171, 210, 256
ApoI RAATTY 2 cut(s) 800, 812
ArsI GACNNNNNNTTYG 2 cut(s) 568, 600
AsiGI ACCGGT 2 cut(s) 248, 623
AspLEI GCGC 5 cut(s) 86, 132, 161, 215, 491
AspS9I GGNCC 5 cut(s) 33, 91, 431, 464, 866
AsuHPI GGTGA 6 cut(s) 256, 322, 419, 613, 766, 932
AvaI CYCGRG 1 cut(s) 134
AvaII GGWCC 4 cut(s) 91, 431, 464, 866
AxyI CCTNAGG 1 cut(s) 435
BaeGI GKGCMC 1 cut(s) 27
BanI GGYRCC 1 cut(s) 158
BauI CACGAG 1 cut(s) 26
BbsI GAAGAC 1 cut(s) 504
BbvI GCAGC 3 cut(s) 158, 222, 268
BccI CCATC 4 cut(s) 488, 599, 890, 920
BceAI ACGGC 6 cut(s) 79, 139, 146, 172, 308, 695
BciVI GTATCC 1 cut(s) 649
BclI TGATCA 2 cut(s) 733, 784
BcoDI GTCTC 3 cut(s) 180, 521, 726
BfaI CTAG 1 cut(s) 660
BfoI RGCGCY 4 cut(s) 133, 162, 216, 492
BfrI CTTAAG 1 cut(s) 542
BfuI GTATCC 1 cut(s) 649
BglI GCCNNNNNGGC 1 cut(s) 156
BisI GCNGC 4 cut(s) 148, 172, 211, 257
BlsI GCNGC 4 cut(s) 149, 173, 212, 258
Bme18I GGWCC 4 cut(s) 91, 431, 464, 866
BmeRI GACNNNNNGTC 2 cut(s) 355, 696
BmeT110I CYCGRG 1 cut(s) 134
BmgBI CACGTC 1 cut(s) 370
BmgT120I GGNCC 5 cut(s) 33, 91, 431, 464, 866
BmiI GGNNCC 4 cut(s) 35, 160, 432, 465
BmsI GCATC 2 cut(s) 751, 773
BpiI GAAGAC 1 cut(s) 504
BpuEI CTTGAG 2 cut(s) 410, 895
BsaAI YACGTR 1 cut(s) 688
BsaHI GRCGYC 1 cut(s) 159
BsaJI CCNNGG 3 cut(s) 87, 133, 145
BsaWI WCCGGW 3 cut(s) 248, 556, 623
Bsc4I CCNNNNNNNGG 5 cut(s) 108, 234, 346, 563, 651
Bse118I RCCGGY 4 cut(s) 206, 248, 283, 623
Bse1I ACTGG 2 cut(s) 535, 701
Bse21I CCTNAGG 1 cut(s) 435
Bse3DI GCAATG 1 cut(s) 723
BseAI TCCGGA 1 cut(s) 556
BseDI CCNNGG 3 cut(s) 87, 133, 145
BseGI GGATG 3 cut(s) 610, 766, 931
BseLI CCNNNNNNNGG 5 cut(s) 108, 234, 346, 563, 651
BseMI GCAATG 1 cut(s) 723
BseMII CTCAG 2 cut(s) 449, 516
BseNI ACTGG 2 cut(s) 535, 701
BseRI GAGGAG 2 cut(s) 62, 714
BseSI GKGCMC 1 cut(s) 27
BseXI GCAGC 3 cut(s) 158, 222, 268
Bsh1236I CGCG 1 cut(s) 147
BshFI GGCC 3 cut(s) 7, 34, 150
BshNI GGYRCC 1 cut(s) 158
BshTI ACCGGT 2 cut(s) 248, 623
BsiHKCI CYCGRG 1 cut(s) 134
BsiSI CCGG 7 cut(s) 207, 249, 284, 384, 492, 557, 624
BslFI GGGAC 3 cut(s) 74, 151, 444
BslI CCNNNNNNNGG 5 cut(s) 108, 234, 346, 563, 651
BsmAI GTCTC 3 cut(s) 180, 521, 726
BsmFI GGGAC 3 cut(s) 74, 151, 444
BsnI GGCC 3 cut(s) 7, 34, 150
BsoBI CYCGRG 1 cut(s) 134
Bsp1286I GDGCHC 1 cut(s) 27
Bsp13I TCCGGA 1 cut(s) 556
Bsp143I GATC 5 cut(s) 388, 397, 553, 733, 784
BspACI CCGC 6 cut(s) 145, 147, 192, 196, 225, 915
BspANI GGCC 3 cut(s) 7, 34, 150
BspCNI CTCAG 2 cut(s) 448, 517
BspEI TCCGGA 1 cut(s) 556
BspFNI CGCG 1 cut(s) 147
BspLI GGNNCC 4 cut(s) 35, 160, 432, 465
BspPI GGATC 2 cut(s) 383, 548
BspT107I GGYRCC 1 cut(s) 158
BspTI CTTAAG 1 cut(s) 542
BsrDI GCAATG 1 cut(s) 723
BsrFI RCCGGY 4 cut(s) 206, 248, 283, 623
BsrI ACTGG 2 cut(s) 535, 701
BssAI RCCGGY 4 cut(s) 206, 248, 283, 623
BssECI CCNNGG 3 cut(s) 87, 133, 145
BssMI GATC 5 cut(s) 388, 397, 553, 733, 784
BssNI GRCGYC 1 cut(s) 159
BssSI CACGAG 1 cut(s) 26
BssT1I CCWWGG 1 cut(s) 87
Bst2BI CACGAG 1 cut(s) 26
Bst4CI ACNGT 2 cut(s) 106, 772
Bst6I CTCTTC 1 cut(s) 80
BstACI GRCGYC 1 cut(s) 159
BstAFI CTTAAG 1 cut(s) 542
BstBAI YACGTR 1 cut(s) 688
BstC8I GCNNGC 5 cut(s) 128, 196, 285, 321, 591
BstDEI CTNAG 2 cut(s) 435, 525
BstDSI CCRYGG 1 cut(s) 145
BstF5I GGATG 3 cut(s) 610, 766, 931
BstFNI CGCG 1 cut(s) 147
BstH2I RGCGCY 4 cut(s) 133, 162, 216, 492
BstHHI GCGC 5 cut(s) 86, 132, 161, 215, 491
BstKTI GATC 5 cut(s) 391, 400, 556, 736, 787
BstMAI GTCTC 3 cut(s) 180, 521, 726
BstMBI GATC 5 cut(s) 388, 397, 553, 733, 784
BstMWI GCNNNNNNNGC 2 cut(s) 31, 156
BstSLI GKGCMC 1 cut(s) 27
BstUI CGCG 1 cut(s) 147
BstV1I GCAGC 3 cut(s) 158, 222, 268
BstV2I GAAGAC 1 cut(s) 504
BstX2I RGATCY 1 cut(s) 388
BstYI RGATCY 1 cut(s) 388
Bsu36I CCTNAGG 1 cut(s) 435
BsuI GTATCC 1 cut(s) 649
BsuRI GGCC 3 cut(s) 7, 34, 150
BtgI CCRYGG 1 cut(s) 145
BtgZI GCGATG 1 cut(s) 189
BtrI CACGTC 1 cut(s) 370
BtsCI GGATG 3 cut(s) 610, 766, 931
Cac8I GCNNGC 5 cut(s) 128, 196, 285, 321, 591
CfoI GCGC 5 cut(s) 86, 132, 161, 215, 491
Cfr10I RCCGGY 4 cut(s) 206, 248, 283, 623
Cfr13I GGNCC 5 cut(s) 33, 91, 431, 464, 866
Cfr42I CCGCGG 1 cut(s) 148
CseI GACGC 2 cut(s) 509, 686
Csp6I GTAC 1 cut(s) 743
CspAI ACCGGT 2 cut(s) 248, 623
CviAII CATG 2 cut(s) 346, 737
CviQI GTAC 1 cut(s) 743
DdeI CTNAG 2 cut(s) 435, 525
DinI GGCGCC 1 cut(s) 160
DpnI GATC 5 cut(s) 390, 399, 555, 735, 786
DpnII GATC 5 cut(s) 388, 397, 553, 733, 784
DrdI GACNNNNNNGTC 1 cut(s) 305
DriI GACNNNNNGTC 2 cut(s) 355, 696
DseDI GACNNNNNNGTC 1 cut(s) 305
EaeI YGGCCR 1 cut(s) 148
Eam1104I CTCTTC 1 cut(s) 80
Eam1105I GACNNNNNGTC 2 cut(s) 355, 696
EarI CTCTTC 1 cut(s) 80
EciI GGCGGA 1 cut(s) 181
Eco130I CCWWGG 1 cut(s) 87
Eco47I GGWCC 4 cut(s) 91, 431, 464, 866
Eco81I CCTNAGG 1 cut(s) 435
Eco88I CYCGRG 1 cut(s) 134
EcoO109I RGGNCCY 3 cut(s) 33, 431, 464
EcoT14I CCWWGG 1 cut(s) 87
EcoT22I ATGCAT 1 cut(s) 766
EgeI GGCGCC 1 cut(s) 160
EheI GGCGCC 1 cut(s) 160
ErhI CCWWGG 1 cut(s) 87
FaeI CATG 2 cut(s) 349, 740
FaiI YATR 7 cut(s) 347, 485, 581, 602, 738, 819, 872
FaqI GGGAC 3 cut(s) 74, 151, 444
FatI CATG 2 cut(s) 345, 736
FauI CCCGC 2 cut(s) 203, 908
FbaI TGATCA 2 cut(s) 733, 784
FblI GTMKAC 2 cut(s) 297, 940
Fnu4HI GCNGC 4 cut(s) 148, 172, 211, 257
FokI GGATG 3 cut(s) 617, 773, 938
Fsp4HI GCNGC 4 cut(s) 148, 172, 211, 257
FspBI CTAG 1 cut(s) 660
GlaI GCGC 5 cut(s) 85, 131, 160, 214, 490
GluI GCNGC 4 cut(s) 148, 172, 211, 257
HaeII RGCGCY 4 cut(s) 133, 162, 216, 492
HaeIII GGCC 3 cut(s) 7, 34, 150
HapII CCGG 7 cut(s) 207, 249, 284, 384, 492, 557, 624
HgaI GACGC 2 cut(s) 509, 686
HhaI GCGC 5 cut(s) 86, 132, 161, 215, 491
Hin1I GRCGYC 1 cut(s) 159
Hin1II CATG 2 cut(s) 349, 740
Hin6I GCGC 5 cut(s) 84, 130, 159, 213, 489
HinP1I GCGC 5 cut(s) 84, 130, 159, 213, 489
HincII GTYRAC 2 cut(s) 298, 669
HindII GTYRAC 2 cut(s) 298, 669
HinfI GANTC 8 cut(s) 188, 278, 364, 410, 521, 670, 826, 953
HpaII CCGG 7 cut(s) 207, 249, 284, 384, 492, 557, 624
HphI GGTGA 6 cut(s) 256, 322, 419, 613, 766, 932
Hpy166II GTNNAC 5 cut(s) 298, 536, 611, 669, 941
Hpy188I TCNGA 6 cut(s) 246, 402, 420, 471, 526, 958
Hpy188III TCNNGA 9 cut(s) 136, 183, 392, 557, 679, 758, 833, 893, 973
Hpy8I GTNNAC 5 cut(s) 298, 536, 611, 669, 941
Hpy99I CGWCG 2 cut(s) 311, 361
HpyAV CCTTC 1 cut(s) 277
HpyCH4III ACNGT 2 cut(s) 106, 772
HpyCH4IV ACGT 7 cut(s) 240, 351, 356, 369, 665, 687, 933
HpyCH4V TGCA 2 cut(s) 716, 764
HpyF10VI GCNNNNNNNGC 2 cut(s) 31, 156
HpyF3I CTNAG 2 cut(s) 435, 525
HpySE526I ACGT 7 cut(s) 240, 351, 356, 369, 665, 687, 933
Hsp92I GRCGYC 1 cut(s) 159
Hsp92II CATG 2 cut(s) 349, 740
HspAI GCGC 5 cut(s) 84, 130, 159, 213, 489
KasI GGCGCC 1 cut(s) 158
Kpn2I TCCGGA 1 cut(s) 556
KroI GCCGGC 1 cut(s) 283
KroNI GCCGGC 1 cut(s) 285
Ksp22I TGATCA 2 cut(s) 733, 784
KspI CCGCGG 1 cut(s) 148
Kzo9I GATC 5 cut(s) 388, 397, 553, 733, 784
Lsp1109I GCAGC 3 cut(s) 158, 222, 268
LweI GCATC 2 cut(s) 751, 773
MaeI CTAG 1 cut(s) 660
MaeII ACGT 7 cut(s) 240, 351, 356, 369, 665, 687, 933
MaeIII GTNAC 5 cut(s) 352, 566, 688, 772, 929
MalI GATC 5 cut(s) 390, 399, 555, 735, 786
MboI GATC 5 cut(s) 388, 397, 553, 733, 784
MboII GAAGA 6 cut(s) 67, 407, 453, 509, 821, 848
MflI RGATCY 1 cut(s) 388
MhlI GDGCHC 1 cut(s) 27
MluCI AATT 5 cut(s) 271, 444, 800, 812, 840
Mly113I GGCGCC 1 cut(s) 159
MlyI GAGTC 3 cut(s) 182, 272, 664
Mph1103I ATGCAT 1 cut(s) 766
MreI CGCCGGCG 1 cut(s) 283
MroI TCCGGA 1 cut(s) 556
MroNI GCCGGC 1 cut(s) 283
MseI TTAA 4 cut(s) 447, 543, 852, 888
MspA1I CMGCKG 1 cut(s) 147
MspCI CTTAAG 1 cut(s) 542
MspI CCGG 7 cut(s) 207, 249, 284, 384, 492, 557, 624
MvnI CGCG 1 cut(s) 147
MwoI GCNNNNNNNGC 2 cut(s) 31, 156
NaeI GCCGGC 1 cut(s) 285
NarI GGCGCC 1 cut(s) 159
NdeII GATC 5 cut(s) 388, 397, 553, 733, 784
NgoMIV GCCGGC 1 cut(s) 283
NlaIII CATG 2 cut(s) 349, 740
NlaIV GGNNCC 4 cut(s) 35, 160, 432, 465
NmuCI GTSAC 3 cut(s) 566, 688, 772
NsiI ATGCAT 1 cut(s) 766
PcsI WCGNNNNNNNCGW 1 cut(s) 303
PdiI GCCGGC 1 cut(s) 285
PfeI GAWTC 5 cut(s) 364, 410, 521, 826, 953
PflMI CCANNNNNTGG 3 cut(s) 108, 234, 651
PinAI ACCGGT 2 cut(s) 248, 623
PkrI GCNGC 4 cut(s) 149, 173, 212, 258
PleI GAGTC 3 cut(s) 182, 272, 664
PluTI GGCGCC 1 cut(s) 162
PpsI GAGTC 3 cut(s) 182, 272, 664
Ppu21I YACGTR 1 cut(s) 688
PpuMI RGGWCCY 2 cut(s) 431, 464
Psp1406I AACGTT 2 cut(s) 240, 665
Psp5II RGGWCCY 2 cut(s) 431, 464
PspN4I GGNNCC 4 cut(s) 35, 160, 432, 465
PspPI GGNCC 5 cut(s) 33, 91, 431, 464, 866
PspPPI RGGWCCY 2 cut(s) 431, 464
PsuI RGATCY 1 cut(s) 388
RsaI GTAC 1 cut(s) 744
RsaNI GTAC 1 cut(s) 743
SacII CCGCGG 1 cut(s) 148
SalI GTCGAC 1 cut(s) 296
SaqAI TTAA 4 cut(s) 447, 543, 852, 888
SatI GCNGC 4 cut(s) 148, 172, 211, 257
Sau3AI GATC 5 cut(s) 388, 397, 553, 733, 784
Sau96I GGNCC 5 cut(s) 33, 91, 431, 464, 866
SchI GAGTC 3 cut(s) 182, 272, 664
SduI GDGCHC 1 cut(s) 27
SfaNI GCATC 2 cut(s) 751, 773
SfoI GGCGCC 1 cut(s) 160
Sfr303I CCGCGG 1 cut(s) 148
SgrAI CRCCGGYG 1 cut(s) 283
SgrBI CCGCGG 1 cut(s) 148
SinI GGWCC 4 cut(s) 91, 431, 464, 866
SmlI CTYRAG 3 cut(s) 425, 542, 910
SmoI CTYRAG 3 cut(s) 425, 542, 910
Sse9I AATT 5 cut(s) 271, 444, 800, 812, 840
SsiI CCGC 6 cut(s) 145, 147, 192, 196, 225, 915
SspDI GGCGCC 1 cut(s) 158
SspMI CTAG 1 cut(s) 660
StyI CCWWGG 1 cut(s) 87
TaaI ACNGT 2 cut(s) 106, 772
TaiI ACGT 7 cut(s) 243, 354, 359, 372, 668, 690, 936
TaqI TCGA 1 cut(s) 297
TaqII GACCGA 1 cut(s) 707
TasI AATT 5 cut(s) 271, 444, 800, 812, 840
TauI GCSGC 1 cut(s) 150
TfiI GAWTC 5 cut(s) 364, 410, 521, 826, 953
Tru1I TTAA 4 cut(s) 447, 543, 852, 888
Tru9I TTAA 4 cut(s) 447, 543, 852, 888
TseFI GTSAC 3 cut(s) 566, 688, 772
TseI GCWGC 3 cut(s) 171, 210, 256
Tsp45I GTSAC 3 cut(s) 566, 688, 772
TspDTI ATGAA 2 cut(s) 32, 334
TspGWI ACGGA 1 cut(s) 295
Van91I CCANNNNNTGG 3 cut(s) 108, 234, 651
Vha464I CTTAAG 1 cut(s) 542
VpaK11BI GGWCC 4 cut(s) 91, 431, 464, 866
XapI RAATTY 2 cut(s) 800, 812
XmiI GTMKAC 2 cut(s) 297, 940
XspI CTAG 1 cut(s) 660
Zsp2I ATGCAT 1 cut(s) 766
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.