Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr00

Orthogroup ID

OG0003764

Physical Location & Seq

Genomic Coordinates Forward (+)

21142159 .. 21142599

Gene Structure 441 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD00G1099900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 441 bp

ATGAGGCCGATACTGATGAAGGGGCACGAGAGGCCCTTGACCTTCCTCAAGTACAACAGGGACGGCGACCTCCTCTTCTCTTGCGCCAAGGATCACAAACCCACCGTCTGGTTCGCCGACAATGGCGAGCACCTCGGCACCTACCGCGGCCACAATGGCGCCGTTTGGTGCTGCGACGTTTCCCGGGACTCCTCCCGCCTCATCACTGGCAGCGCTGATCAGACCGCCAAGCTCTGGGACGTTCAGACCGGCCGACTGCTCTTCACCTTCAACTTTGACTCCCCGGCCAGGGCTGTTGACTTATCCGTCGGGGATCGGCTGGCTGTTATCACCACCGACCCCTTCATGGGGGTCACTTCCGCGATTCACGTCAAGCGCATTGACAGAGACCCCGAAGATCGTAACAATAATCTCCTCTTATTTGCCTTGCCCTGTTTTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000151 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006950 GO:0006970 GO:0008150 GO:0009628 GO:0009651 GO:0010035 GO:0010038 GO:0031461 GO:0032991 GO:0042221 GO:0044424 GO:0044444 GO:0044464 GO:0046686 GO:0050896 GO:0080008 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

ko03013 map03013

Pfam Domains

Protein Families

WD40

Protein Analysis

147

Amino Acids

16.47

Weight (kDa)

6.95

Isoelectric Point (pI)

28.26

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
EIF3I	PF24805	1 - 136	5e-53	EIF3I
Beta-prop_WDR3_1st	PF25173	5 - 90	8.4e-17	WDR3 first beta-propeller domain
Beta-prop_WDR5	PF25175	6 - 103	5.6e-17	WDR5 beta-propeller domain
WD40_Prp19	PF24814	6 - 92	3.3e-09	Prp19 WD40 domain
WD40_CDC20-Fz	PF24807	6 - 94	9.6e-09	CDC20/Fizzy WD40 domain
Beta-prop_WDR3_2nd	PF25172	7 - 80	1.2e-10	WDR3 second beta-propeller domain
WDR55	PF24796	7 - 90	2.3e-06	WDR55
WD40_Gbeta	PF25391	8 - 90	2.3e-07	G protein beta WD-40 repeat protein
Beta-prop_WDR36-Utp21_2nd	PF25168	11 - 109	4.1e-09	WDR36/Utp21 second beta-propeller domain
Beta-prop_THOC3	PF25174	12 - 121	3.7e-17	THOC3 beta-propeller domain
WD40_WDHD1_1st	PF24817	12 - 91	1.4e-06	WDHD1 first WD40 domain
WD40	PF00400	42 - 80	5.8e-11	WD domain, G-beta repeat
Beta-prop_TEP1_2nd	PF25047	47 - 84	6.4e-07	TEP-1 second beta-propeller

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

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Genomic Context

Gene Family Tree

Species Filter

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Orthologous Genes (Group: OG0003764)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G46280 AT2G46280 AT2G46290
fragaria_vesca	FvH4_4g35950
malus_domestica	MD00G1099900.v1.1 MD13G1013500.v1.1 MD15G1352700.v1.1 MD16G1010400.v1.1
prunus_persica	Prupe.1G340200_v2.0.a1 Prupe.3G206800_v2.0.a1
pyrus_communis	pycom02g16760 pycom13g01140 pycom16g01020
rosa_chinensis	RchiOBHm_Chr4g0445591 RchiOBHm_Chr5g0012021
rosa_laevigata	RLG00000005728 RLG00000031879
rosa_multiflora	Rmu_sc0010148.1_g000009 Rmu_sc0023810.1_g000001 Rmu_ssc0000253.1_g000008
rosa_roxburghii	Rroxscaffold_1G00064570 Rroxscaffold_5G00386060
rosa_rugosa	Rorug04G0362700 Rorug04G0460400
rosa_samantha	Rh4AG424100 Rh4BG436200 Rh4CG450800 Rh4DG432700 Rh5AG092500 Rh5BG088300 Rh5CG102100 Rh5DG088800
rosa_wichuraiana	Rw4G036360 Rw5G008070

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	305
AccB1I	GGYRCC	2 cut(s)	137, 158
AccB7I	CCANNNNNTGG	2 cut(s)	108, 234
AccII	CGCG	2 cut(s)	147, 362
AciI	CCGC	5 cut(s)	145, 147, 196, 225, 360
AclWI	GGATC	2 cut(s)	99, 321
AcoI	YGGCCR	3 cut(s)	148, 250, 285
AcyI	GRCGYC	1 cut(s)	159
AfaI	GTAC	1 cut(s)	53
AfeI	AGCGCT	1 cut(s)	214
AfiI	CCNNNNNNNGG	7 cut(s)	108, 234, 288, 289, 346, 347, 348
AgsI	TTSAA	1 cut(s)	271
AjiI	CACGTC	1 cut(s)	370
AjnI	CCWGG	1 cut(s)	287
AluBI	AGCT	1 cut(s)	232
AluI	AGCT	1 cut(s)	232
Alw21I	GWGCWC	1 cut(s)	132
Alw26I	GTCTC	1 cut(s)	381
AlwI	GGATC	2 cut(s)	99, 321
Ama87I	CYCGRG	1 cut(s)	183
Aor51HI	AGCGCT	1 cut(s)	214
AoxI	GGCC	5 cut(s)	5, 32, 148, 250, 285
ApeKI	GCWGC	2 cut(s)	171, 210
ArsI	GACNNNNNNTTYG	2 cut(s)	90, 122
AspLEI	GCGC	4 cut(s)	86, 161, 215, 378
AspS9I	GGNCC	1 cut(s)	33
AsuC2I	CCSGG	3 cut(s)	184, 185, 284
AsuHPI	GGTGA	2 cut(s)	256, 322
AvaI	CYCGRG	1 cut(s)	183
BaeGI	GKGCMC	1 cut(s)	27
BanI	GGYRCC	2 cut(s)	137, 158
BauI	CACGAG	1 cut(s)	26
Bbv12I	GWGCWC	1 cut(s)	132
BbvI	GCAGC	2 cut(s)	158, 222
BceAI	ACGGC	2 cut(s)	79, 146
BciT130I	CCWGG	1 cut(s)	289
BclI	TGATCA	1 cut(s)	217
BcnI	CCSGG	3 cut(s)	184, 185, 284
BcoDI	GTCTC	1 cut(s)	381
BfoI	RGCGCY	2 cut(s)	162, 216
BglI	GCCNNNNNGGC	1 cut(s)	156
BisI	GCNGC	3 cut(s)	148, 172, 211
BlsI	GCNGC	3 cut(s)	149, 173, 212
Bme1390I	CCNGG	4 cut(s)	184, 185, 284, 289
BmeT110I	CYCGRG	1 cut(s)	183
BmgBI	CACGTC	1 cut(s)	370
BmgT120I	GGNCC	1 cut(s)	33
BmiI	GGNNCC	2 cut(s)	139, 160
BmrFI	CCNGG	4 cut(s)	184, 185, 284, 289
BpuEI	CTTGAG	1 cut(s)	32
BpuMI	CCSGG	3 cut(s)	184, 185, 284
BsaHI	GRCGYC	1 cut(s)	159
BsaI	GGTCTC	1 cut(s)	381
BsaJI	CCNNGG	6 cut(s)	87, 133, 145, 183, 282, 288
BsaXI	ACNNNNNCTCC	2 cut(s)	263, 293
Bsc4I	CCNNNNNNNGG	7 cut(s)	108, 234, 288, 289, 346, 347, 348
Bse118I	RCCGGY	1 cut(s)	248
Bse1I	ACTGG	1 cut(s)	211
BseBI	CCWGG	1 cut(s)	289
BseDI	CCNNGG	6 cut(s)	87, 133, 145, 183, 282, 288
BseLI	CCNNNNNNNGG	7 cut(s)	108, 234, 288, 289, 346, 347, 348
BseNI	ACTGG	1 cut(s)	211
BseRI	GAGGAG	3 cut(s)	62, 181, 404
BseSI	GKGCMC	1 cut(s)	27
BseX3I	CGGCCG	1 cut(s)	250
BseXI	GCAGC	2 cut(s)	158, 222
Bsh1236I	CGCG	2 cut(s)	147, 362
Bsh1285I	CGRYCG	1 cut(s)	253
BshFI	GGCC	5 cut(s)	7, 34, 150, 252, 287
BshNI	GGYRCC	2 cut(s)	137, 158
BsiEI	CGRYCG	1 cut(s)	253
BsiHKAI	GWGCWC	1 cut(s)	132
BsiHKCI	CYCGRG	1 cut(s)	183
BsiSI	CCGG	3 cut(s)	184, 249, 284
BslFI	GGGAC	3 cut(s)	74, 200, 251
BslI	CCNNNNNNNGG	7 cut(s)	108, 234, 288, 289, 346, 347, 348
BsmAI	GTCTC	1 cut(s)	381
BsmFI	GGGAC	3 cut(s)	74, 200, 251
BsnI	GGCC	5 cut(s)	7, 34, 150, 252, 287
Bso31I	GGTCTC	1 cut(s)	381
BsoBI	CYCGRG	1 cut(s)	183
Bsp1286I	GDGCHC	2 cut(s)	27, 132
Bsp143I	GATC	4 cut(s)	91, 217, 313, 397
BspACI	CCGC	5 cut(s)	145, 147, 196, 225, 360
BspANI	GGCC	5 cut(s)	7, 34, 150, 252, 287
BspFNI	CGCG	2 cut(s)	147, 362
BspLI	GGNNCC	2 cut(s)	139, 160
BspPI	GGATC	2 cut(s)	99, 321
BspQI	GCTCTTC	1 cut(s)	266
BspT107I	GGYRCC	2 cut(s)	137, 158
BspTNI	GGTCTC	1 cut(s)	381
BsrFI	RCCGGY	1 cut(s)	248
BsrI	ACTGG	1 cut(s)	211
BssAI	RCCGGY	1 cut(s)	248
BssECI	CCNNGG	6 cut(s)	87, 133, 145, 183, 282, 288
BssMI	GATC	4 cut(s)	91, 217, 313, 397
BssNI	GRCGYC	1 cut(s)	159
BssSI	CACGAG	1 cut(s)	26
BssT1I	CCWWGG	1 cut(s)	87
Bst2BI	CACGAG	1 cut(s)	26
Bst2UI	CCWGG	1 cut(s)	289
Bst4CI	ACNGT	1 cut(s)	106
Bst6I	CTCTTC	2 cut(s)	80, 266
BstACI	GRCGYC	1 cut(s)	159
BstC8I	GCNNGC	2 cut(s)	128, 321
BstDSI	CCRYGG	1 cut(s)	145
BstFNI	CGCG	2 cut(s)	147, 362
BstH2I	RGCGCY	2 cut(s)	162, 216
BstHHI	GCGC	4 cut(s)	86, 161, 215, 378
BstKTI	GATC	4 cut(s)	94, 220, 316, 400
BstMAI	GTCTC	1 cut(s)	381
BstMBI	GATC	4 cut(s)	91, 217, 313, 397
BstMCI	CGRYCG	1 cut(s)	253
BstMWI	GCNNNNNNNGC	3 cut(s)	31, 144, 156
BstNI	CCWGG	1 cut(s)	289
BstSCI	CCNGG	4 cut(s)	182, 183, 282, 287
BstSLI	GKGCMC	1 cut(s)	27
BstUI	CGCG	2 cut(s)	147, 362
BstV1I	GCAGC	2 cut(s)	158, 222
BstZI	CGGCCG	1 cut(s)	250
BsuRI	GGCC	5 cut(s)	7, 34, 150, 252, 287
BtgI	CCRYGG	1 cut(s)	145
BtrI	CACGTC	1 cut(s)	370
BtsIMutI	CAGTG	1 cut(s)	204
Cac8I	GCNNGC	2 cut(s)	128, 321
CfoI	GCGC	4 cut(s)	86, 161, 215, 378
Cfr10I	RCCGGY	1 cut(s)	248
Cfr13I	GGNCC	1 cut(s)	33
Cfr42I	CCGCGG	1 cut(s)	148
Cfr9I	CCCGGG	1 cut(s)	183
Csp6I	GTAC	1 cut(s)	52
CviAII	CATG	1 cut(s)	346
CviJI	RGCY	9 cut(s)	7, 34, 150, 232, 252, 287, 293, 319, 323
CviKI_1	RGCY	9 cut(s)	7, 34, 150, 232, 252, 287, 293, 319, 323
CviQI	GTAC	1 cut(s)	52
DinI	GGCGCC	1 cut(s)	160
DpnI	GATC	4 cut(s)	93, 219, 315, 399
DpnII	GATC	4 cut(s)	91, 217, 313, 397
DrdI	GACNNNNNNGTC	1 cut(s)	305
DseDI	GACNNNNNNGTC	1 cut(s)	305
EaeI	YGGCCR	3 cut(s)	148, 250, 285
EagI	CGGCCG	1 cut(s)	250
Eam1104I	CTCTTC	2 cut(s)	80, 266
EarI	CTCTTC	2 cut(s)	80, 266
EclXI	CGGCCG	1 cut(s)	250
Eco130I	CCWWGG	1 cut(s)	87
Eco31I	GGTCTC	1 cut(s)	381
Eco47III	AGCGCT	1 cut(s)	214
Eco52I	CGGCCG	1 cut(s)	250
Eco88I	CYCGRG	1 cut(s)	183
EcoO109I	RGGNCCY	1 cut(s)	33
EcoRII	CCWGG	1 cut(s)	287
EcoT14I	CCWWGG	1 cut(s)	87
EgeI	GGCGCC	1 cut(s)	160
EheI	GGCGCC	1 cut(s)	160
ErhI	CCWWGG	1 cut(s)	87
FaeI	CATG	1 cut(s)	349
FaiI	YATR	1 cut(s)	347
FaqI	GGGAC	3 cut(s)	74, 200, 251
FatI	CATG	1 cut(s)	345
FauI	CCCGC	1 cut(s)	203
FbaI	TGATCA	1 cut(s)	217
Fnu4HI	GCNGC	3 cut(s)	148, 172, 211
Fsp4HI	GCNGC	3 cut(s)	148, 172, 211
GlaI	GCGC	4 cut(s)	85, 160, 214, 377
GluI	GCNGC	3 cut(s)	148, 172, 211
HaeII	RGCGCY	2 cut(s)	162, 216
HaeIII	GGCC	5 cut(s)	7, 34, 150, 252, 287
HapII	CCGG	3 cut(s)	184, 249, 284
HhaI	GCGC	4 cut(s)	86, 161, 215, 378
Hin1I	GRCGYC	1 cut(s)	159
Hin1II	CATG	1 cut(s)	349
Hin6I	GCGC	4 cut(s)	84, 159, 213, 376
HinP1I	GCGC	4 cut(s)	84, 159, 213, 376
HincII	GTYRAC	1 cut(s)	298
HindII	GTYRAC	1 cut(s)	298
HinfI	GANTC	3 cut(s)	188, 278, 364
HpaII	CCGG	3 cut(s)	184, 249, 284
HphI	GGTGA	2 cut(s)	256, 322
Hpy166II	GTNNAC	1 cut(s)	298
Hpy188I	TCNGA	2 cut(s)	222, 246
Hpy8I	GTNNAC	1 cut(s)	298
Hpy99I	CGWCG	2 cut(s)	179, 311
HpyAV	CCTTC	4 cut(s)	13, 52, 277, 352
HpyCH4III	ACNGT	1 cut(s)	106
HpyCH4IV	ACGT	3 cut(s)	177, 240, 369
HpyF10VI	GCNNNNNNNGC	3 cut(s)	31, 144, 156
HpySE526I	ACGT	3 cut(s)	177, 240, 369
Hsp92I	GRCGYC	1 cut(s)	159
Hsp92II	CATG	1 cut(s)	349
HspAI	GCGC	4 cut(s)	84, 159, 213, 376
KasI	GGCGCC	1 cut(s)	158
Ksp22I	TGATCA	1 cut(s)	217
KspI	CCGCGG	1 cut(s)	148
Kzo9I	GATC	4 cut(s)	91, 217, 313, 397
LguI	GCTCTTC	1 cut(s)	266
Lsp1109I	GCAGC	2 cut(s)	158, 222
MaeII	ACGT	3 cut(s)	177, 240, 369
MaeIII	GTNAC	2 cut(s)	352, 401
MalI	GATC	4 cut(s)	93, 219, 315, 399
MboI	GATC	4 cut(s)	91, 217, 313, 397
MboII	GAAGA	3 cut(s)	67, 253, 407
MhlI	GDGCHC	2 cut(s)	27, 132
Mly113I	GGCGCC	1 cut(s)	159
MlyI	GAGTC	2 cut(s)	182, 272
MnlI	CCTC	8 cut(s)	24, 56, 80, 83, 143, 202, 209, 425
MspA1I	CMGCKG	1 cut(s)	147
MspI	CCGG	3 cut(s)	184, 249, 284
MspR9I	CCNGG	4 cut(s)	184, 185, 284, 289
MvaI	CCWGG	1 cut(s)	289
MvnI	CGCG	2 cut(s)	147, 362
MwoI	GCNNNNNNNGC	3 cut(s)	31, 144, 156
NarI	GGCGCC	1 cut(s)	159
NciI	CCSGG	3 cut(s)	184, 185, 284
NdeII	GATC	4 cut(s)	91, 217, 313, 397
NlaIII	CATG	1 cut(s)	349
NlaIV	GGNNCC	2 cut(s)	139, 160
NmeAIII	GCCGAG	1 cut(s)	114
NmuCI	GTSAC	1 cut(s)	352
PciSI	GCTCTTC	1 cut(s)	266
PfeI	GAWTC	1 cut(s)	364
PflMI	CCANNNNNTGG	2 cut(s)	108, 234
PkrI	GCNGC	3 cut(s)	149, 173, 212
PleI	GAGTC	2 cut(s)	182, 272
PluTI	GGCGCC	1 cut(s)	162
PpsI	GAGTC	2 cut(s)	182, 272
Psp6I	CCWGG	1 cut(s)	287
PspGI	CCWGG	1 cut(s)	287
PspN4I	GGNNCC	2 cut(s)	139, 160
PspPI	GGNCC	1 cut(s)	33
RsaI	GTAC	1 cut(s)	53
RsaNI	GTAC	1 cut(s)	52
SacII	CCGCGG	1 cut(s)	148
SapI	GCTCTTC	1 cut(s)	266
SatI	GCNGC	3 cut(s)	148, 172, 211
Sau3AI	GATC	4 cut(s)	91, 217, 313, 397
Sau96I	GGNCC	1 cut(s)	33
SchI	GAGTC	2 cut(s)	182, 272
ScrFI	CCNGG	4 cut(s)	184, 185, 284, 289
SduI	GDGCHC	2 cut(s)	27, 132
SetI	ASST	9 cut(s)	44, 72, 135, 143, 180, 234, 243, 269, 372
SfoI	GGCGCC	1 cut(s)	160
Sfr303I	CCGCGG	1 cut(s)	148
SgrBI	CCGCGG	1 cut(s)	148
SmaI	CCCGGG	1 cut(s)	185
SmlI	CTYRAG	1 cut(s)	47
SmoI	CTYRAG	1 cut(s)	47
SsiI	CCGC	5 cut(s)	145, 147, 196, 225, 360
SspDI	GGCGCC	1 cut(s)	158
StyD4I	CCNGG	4 cut(s)	182, 183, 282, 287
StyI	CCWWGG	1 cut(s)	87
TaaI	ACNGT	1 cut(s)	106
TaiI	ACGT	3 cut(s)	180, 243, 372
TatI	WGTACW	1 cut(s)	51
TauI	GCSGC	1 cut(s)	150
TfiI	GAWTC	1 cut(s)	364
TscAI	CASTG	1 cut(s)	211
TseFI	GTSAC	1 cut(s)	352
TseI	GCWGC	2 cut(s)	171, 210
Tsp45I	GTSAC	1 cut(s)	352
TspDTI	ATGAA	2 cut(s)	32, 334
TspGWI	ACGGA	1 cut(s)	295
TspMI	CCCGGG	1 cut(s)	183
TspRI	CASTG	1 cut(s)	211
Van91I	CCANNNNNTGG	2 cut(s)	108, 234
XmaI	CCCGGG	1 cut(s)	183

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.