AT2G46290

component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0003764
Physical Location & Seq
Reverse (-)
19005771 .. 19007952
2182 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G46290.1

Sequence Viewer

Length: 1068 bp
ATGACAATTGAGTGCGTTAATCCCGAAGGTCTTCGATTCAACCAAATGAAAAGCTCCTCTCATCAAACCCTAGAAACGAGGATGAGGCCGATTTTGATGAAGGGACATGAACGTCCATTGACGTTCCTGAGGTACAACAGAAATGGTGATCTGCTTTTCTCCTGCGCCAAAGACCACACTCCCACAGTCTGGTTTGCCGATAACGGCGAGCGTCTTGGCACTTACCGTGGCCACAGTGGTGCTGTTTGGTGCTGTGATATCTCCAGAGACTCGTCTAGATTGATCACTGGTAGTGCTGATCAGACTGCAAAGCTGTGGGATGTGAAATCTGGCAAAGAATTGTTCACTTTCAAGTTTGGTGCCCCTGCAAGGTCTGTGGATTTCTCTGTTGGTGATCATCTTGCAGTGATTACCACTGATCACTTCGTGGGAACTTCCTCTGCTATTCATGTCAAACGCATTGCAGAAGATCCCGAAGACCAGGTTGGTGATTCTGTGCTTGTCCTTCAAAGTCCTGATGGAAAGAAGAAGATCAATAGAGCTGTTTGGGGTCCCCTGAACCAAACCATTGTTAGTGGTGGTGAAGATGCTGCTATCAGAATCTGGGATGCAGAGACTGGAAAATTGCTTAAGCAATCAGATGAGGAAGTGGGTCACAAGGAGGCCATTACATCCCTCTGCAAAGCAGCTGATGACTCTCACTTCCTTACAGGTTCACATGACAAAACTGCAAAGCTTTGGGACATGAGAACGCTGACTCTTATTAAGACTTACACCACTGTGGTGCCTGTAAATGCTGTCGCCATGTCTCCACTTCTCAACCATGTTGTGCTTGGAGGTGGTCAAGATGCATCAGCTGTGACTACCACTGATCATCGTGCTGGGAAGTTTGAAGCTAAGTTTTACGACACGATTCTGCAAGAGGAAATTGGTGGCGTGAAAGGTCATTTTGGACCTATTAATGCTTTGGCATTCAGTCCTGATGGGAAGAGTTTCTCTAGTGGAGGTGAAGACGGCTACGTGAGACTGCATCATTTTGACTCCAATTACTTCAACATCAAGATTTAG

Protein Analysis

355

Amino Acids

39.0

Weight (kDa)

6.43

Isoelectric Point (pI)

36.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_WDR3_1st PF25173 25 - 117 1.3e-15 WDR3 first beta-propeller domain
EIF3I PF24805 28 - 354 6.4e-127 EIF3I
WD40_CDC20-Fz PF24807 31 - 202 3e-11 CDC20/Fizzy WD40 domain
WDR55 PF24796 33 - 118 7e-06 WDR55
Beta-prop_WDR5 PF25175 33 - 134 1.8e-16 WDR5 beta-propeller domain
WD40_Prp19 PF24814 33 - 118 1.4e-08 Prp19 WD40 domain
Beta-prop_WDR3_2nd PF25172 34 - 107 1e-09 WDR3 second beta-propeller domain
WD40_Gbeta PF25391 35 - 117 1.7e-07 G protein beta WD-40 repeat protein
Beta-prop_THOC3 PF25174 36 - 148 2.1e-17 THOC3 beta-propeller domain
Beta-prop_WDR36-Utp21_2nd PF25168 37 - 139 4.4e-08 WDR36/Utp21 second beta-propeller domain
WD40_WDHD1_1st PF24817 38 - 227 8.7e-13 WDHD1 first WD40 domain
WD40 PF00400 69 - 107 1.1e-10 WD domain, G-beta repeat
Beta-prop_TEP1_2nd PF25047 74 - 112 2.1e-06 TEP-1 second beta-propeller
Beta-prop_EML_2 PF23414 92 - 226 1.5e-06 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_CAF1B_HIR1 PF24105 144 - 276 2.1e-06 CAF1B/HIR1 beta-propeller domain
Beta-prop_WDR3_1st PF25173 175 - 279 2.2e-12 WDR3 first beta-propeller domain
WD40_Prp19 PF24814 175 - 344 3.6e-17 Prp19 WD40 domain
Beta-prop_THOC3 PF25174 175 - 300 4.3e-17 THOC3 beta-propeller domain
WD40_CDC20-Fz PF24807 175 - 291 2.1e-09 CDC20/Fizzy WD40 domain
Beta-prop_WDR5 PF25175 182 - 277 1.4e-13 WDR5 beta-propeller domain
Beta-prop_WDR3_2nd PF25172 182 - 257 9.2e-07 WDR3 second beta-propeller domain
WD40_Gbeta PF25391 187 - 295 2.7e-10 G protein beta WD-40 repeat protein
WD40_WDHD1_1st PF24817 188 - 348 2.5e-09 WDHD1 first WD40 domain
WDR55 PF24796 188 - 259 8.5e-08 WDR55
Beta-prop_WDR36-Utp21_2nd PF25168 195 - 273 6.3e-07 WDR36/Utp21 second beta-propeller domain
WD40 PF00400 218 - 248 1.1e-06 WD domain, G-beta repeat
Beta-prop_EML_2 PF23414 225 - 352 1.5e-10 Echinoderm microtubule-associated protein second beta-propeller
WD40 PF00400 312 - 343 4.7e-07 WD domain, G-beta repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 111
AccB1I GGYRCC 2 cut(s) 359, 784
AccB7I CCANNNNNTGG 1 cut(s) 189
AclWI GGATC 1 cut(s) 464
AcoI YGGCCR 1 cut(s) 229
AdeI CACNNNGTG 1 cut(s) 427
AfaI GTAC 1 cut(s) 134
AfiI CCNNNNNNNGG 2 cut(s) 189, 369
AflII CTTAAG 1 cut(s) 629
AgsI TTSAA 5 cut(s) 40, 352, 509, 893, 1054
AjnI CCWGG 1 cut(s) 480
AjuI GAANNNNNNNTTGG 2 cut(s) 468, 500
AleI CACNNNNGTG 3 cut(s) 237, 779, 782
AluBI AGCT 7 cut(s) 54, 313, 542, 689, 736, 857, 896
AluI AGCT 7 cut(s) 54, 313, 542, 689, 736, 857, 896
Alw26I GTCTC 4 cut(s) 261, 608, 813, 1018
AlwI GGATC 1 cut(s) 464
AlwNI CAGNNNCTG 2 cut(s) 603, 617
AoxI GGCC 3 cut(s) 86, 229, 663
ApeKI GCWGC 2 cut(s) 590, 686
AseI ATTAAT 1 cut(s) 960
Asp700I GAANNNNTTC 2 cut(s) 30, 992
AspLEI GCGC 1 cut(s) 167
AspS9I GGNCC 2 cut(s) 551, 953
AsuHPI GGTGA 5 cut(s) 158, 404, 500, 593, 1019
AvaII GGWCC 2 cut(s) 551, 953
AxyI CCTNAGG 1 cut(s) 128
BaeGI GKGCMC 1 cut(s) 364
BalI TGGCCA 1 cut(s) 231
BanI GGYRCC 2 cut(s) 359, 784
BbsI GAAGAC 3 cut(s) 23, 483, 1017
BbvI GCAGC 2 cut(s) 577, 698
BccI CCATC 2 cut(s) 512, 977
BceAI ACGGC 2 cut(s) 220, 1030
BciT130I CCWGG 1 cut(s) 482
BclI TGATCA 5 cut(s) 282, 298, 394, 418, 871
BcoDI GTCTC 4 cut(s) 261, 608, 813, 1018
BfaI CTAG 3 cut(s) 71, 276, 999
BfrI CTTAAG 1 cut(s) 629
BisI GCNGC 2 cut(s) 591, 687
BlsI GCNGC 2 cut(s) 592, 688
Bme1390I CCNGG 1 cut(s) 482
Bme18I GGWCC 2 cut(s) 551, 953
BmgT120I GGNCC 2 cut(s) 551, 953
BmiI GGNNCC 4 cut(s) 361, 552, 553, 786
BmrFI CCNGG 1 cut(s) 482
BmsI GCATC 5 cut(s) 577, 598, 838, 860, 1039
BpiI GAAGAC 3 cut(s) 23, 483, 1017
BpmI CTGGAG 1 cut(s) 247
BsaAI YACGTR 1 cut(s) 1021
BsaJI CCNNGG 1 cut(s) 226
Bsc4I CCNNNNNNNGG 2 cut(s) 189, 369
Bse1I ACTGG 2 cut(s) 292, 622
Bse21I CCTNAGG 1 cut(s) 128
Bse3DI GCAATG 1 cut(s) 459
BseBI CCWGG 1 cut(s) 482
BseDI CCNNGG 1 cut(s) 226
BseGI GGATG 4 cut(s) 87, 325, 613, 671
BseLI CCNNNNNNNGG 2 cut(s) 189, 369
BseMI GCAATG 1 cut(s) 459
BseMII CTCAG 1 cut(s) 119
BseNI ACTGG 2 cut(s) 292, 622
BseRI GAGGAG 1 cut(s) 46
BseSI GKGCMC 1 cut(s) 364
BseXI GCAGC 2 cut(s) 577, 698
BseYI CCCAGC 1 cut(s) 881
BshFI GGCC 3 cut(s) 88, 231, 665
BshNI GGYRCC 2 cut(s) 359, 784
BslFI GGGAC 3 cut(s) 117, 537, 755
BslI CCNNNNNNNGG 2 cut(s) 189, 369
BsmAI GTCTC 4 cut(s) 261, 608, 813, 1018
BsmFI GGGAC 3 cut(s) 117, 537, 755
BsmI GAATGC 1 cut(s) 971
BsnI GGCC 3 cut(s) 88, 231, 665
Bsp1286I GDGCHC 1 cut(s) 364
Bsp143I GATC 8 cut(s) 148, 282, 298, 394, 418, 469, 531, 871
BspANI GGCC 3 cut(s) 88, 231, 665
BspCNI CTCAG 1 cut(s) 120
BspLI GGNNCC 4 cut(s) 361, 552, 553, 786
BspPI GGATC 1 cut(s) 464
BspT107I GGYRCC 2 cut(s) 359, 784
BspTI CTTAAG 1 cut(s) 629
BsrDI GCAATG 1 cut(s) 459
BsrI ACTGG 2 cut(s) 292, 622
BssECI CCNNGG 1 cut(s) 226
BssMI GATC 8 cut(s) 148, 282, 298, 394, 418, 469, 531, 871
Bst2UI CCWGG 1 cut(s) 482
Bst4CI ACNGT 4 cut(s) 187, 227, 236, 781
Bst6I CTCTTC 1 cut(s) 983
BstAFI CTTAAG 1 cut(s) 629
BstBAI YACGTR 1 cut(s) 1021
BstC8I GCNNGC 1 cut(s) 209
BstDEI CTNAG 2 cut(s) 128, 897
BstDSI CCRYGG 1 cut(s) 226
BstF5I GGATG 4 cut(s) 87, 325, 613, 671
BstHHI GCGC 1 cut(s) 167
BstKTI GATC 8 cut(s) 151, 285, 301, 397, 421, 472, 534, 874
BstMAI GTCTC 4 cut(s) 261, 608, 813, 1018
BstMBI GATC 8 cut(s) 148, 282, 298, 394, 418, 469, 531, 871
BstNI CCWGG 1 cut(s) 482
BstSCI CCNGG 1 cut(s) 480
BstSLI GKGCMC 1 cut(s) 364
BstV1I GCAGC 2 cut(s) 577, 698
BstV2I GAAGAC 3 cut(s) 23, 483, 1017
BstX2I RGATCY 1 cut(s) 469
BstYI RGATCY 1 cut(s) 469
Bsu36I CCTNAGG 1 cut(s) 128
BsuRI GGCC 3 cut(s) 88, 231, 665
BtgI CCRYGG 1 cut(s) 226
BtsCI GGATG 4 cut(s) 87, 325, 613, 671
BtsI GCAGTG 1 cut(s) 411
BtsIMutI CAGTG 6 cut(s) 241, 285, 411, 414, 777, 867
Cac8I GCNNGC 1 cut(s) 209
CaiI CAGNNNCTG 2 cut(s) 603, 617
CfoI GCGC 1 cut(s) 167
Cfr13I GGNCC 2 cut(s) 551, 953
CseI GACGC 1 cut(s) 200
CsiI ACCWGGT 1 cut(s) 480
Csp6I GTAC 1 cut(s) 133
CspCI CAANNNNNGTGG 2 cut(s) 357, 392
CviAII CATG 6 cut(s) 107, 449, 719, 745, 805, 824
CviQI GTAC 1 cut(s) 133
DdeI CTNAG 2 cut(s) 128, 897
DpnI GATC 8 cut(s) 150, 284, 300, 396, 420, 471, 533, 873
DpnII GATC 8 cut(s) 148, 282, 298, 394, 418, 469, 531, 871
DraIII CACNNNGTG 1 cut(s) 427
DrdI GACNNNNNNGTC 1 cut(s) 111
DseDI GACNNNNNNGTC 1 cut(s) 111
EaeI YGGCCR 1 cut(s) 229
Eam1104I CTCTTC 1 cut(s) 983
EarI CTCTTC 1 cut(s) 983
Eco32I GATATC 1 cut(s) 259
Eco47I GGWCC 2 cut(s) 551, 953
Eco81I CCTNAGG 1 cut(s) 128
EcoO109I RGGNCCY 1 cut(s) 551
EcoRII CCWGG 1 cut(s) 480
EcoRV GATATC 1 cut(s) 259
EcoT22I ATGCAT 1 cut(s) 853
FaeI CATG 6 cut(s) 110, 452, 722, 748, 808, 827
FaiI YATR 6 cut(s) 108, 450, 720, 746, 806, 825
FaqI GGGAC 3 cut(s) 117, 537, 755
FatI CATG 6 cut(s) 106, 448, 718, 744, 804, 823
FbaI TGATCA 5 cut(s) 282, 298, 394, 418, 871
Fnu4HI GCNGC 2 cut(s) 591, 687
FokI GGATG 4 cut(s) 94, 332, 620, 658
Fsp4HI GCNGC 2 cut(s) 591, 687
FspBI CTAG 3 cut(s) 71, 276, 999
GlaI GCGC 1 cut(s) 166
GluI GCNGC 2 cut(s) 591, 687
GsaI CCCAGC 1 cut(s) 885
GsuI CTGGAG 1 cut(s) 247
HaeIII GGCC 3 cut(s) 88, 231, 665
HgaI GACGC 1 cut(s) 200
HhaI GCGC 1 cut(s) 167
Hin1II CATG 6 cut(s) 110, 452, 722, 748, 808, 827
Hin6I GCGC 1 cut(s) 165
HinP1I GCGC 1 cut(s) 165
HindIII AAGCTT 1 cut(s) 734
HinfI GANTC 8 cut(s) 36, 269, 491, 600, 695, 757, 913, 1040
HphI GGTGA 5 cut(s) 158, 404, 500, 593, 1019
Hpy166II GTNNAC 2 cut(s) 345, 716
Hpy188I TCNGA 3 cut(s) 303, 599, 640
Hpy188III TCNNGA 9 cut(s) 23, 127, 264, 276, 473, 515, 845, 980, 1060
Hpy8I GTNNAC 2 cut(s) 345, 716
HpyAV CCTTC 3 cut(s) 20, 94, 515
HpyCH4III ACNGT 4 cut(s) 187, 227, 236, 781
HpyCH4IV ACGT 3 cut(s) 112, 122, 1020
HpyF3I CTNAG 2 cut(s) 128, 897
HpySE526I ACGT 3 cut(s) 112, 122, 1020
Hsp92II CATG 6 cut(s) 110, 452, 722, 748, 808, 827
HspAI GCGC 1 cut(s) 165
KflI GGGWCCC 1 cut(s) 551
Ksp22I TGATCA 5 cut(s) 282, 298, 394, 418, 871
Kzo9I GATC 8 cut(s) 148, 282, 298, 394, 418, 469, 531, 871
LmnI GCTCC 1 cut(s) 59
Lsp1109I GCAGC 2 cut(s) 577, 698
LweI GCATC 5 cut(s) 577, 598, 838, 860, 1039
MabI ACCWGGT 1 cut(s) 480
MaeI CTAG 3 cut(s) 71, 276, 999
MaeII ACGT 3 cut(s) 112, 122, 1020
MaeIII GTNAC 2 cut(s) 653, 859
MalI GATC 8 cut(s) 150, 284, 300, 396, 420, 471, 533, 873
MboI GATC 8 cut(s) 148, 282, 298, 394, 418, 469, 531, 871
MboII GAAGA 8 cut(s) 23, 479, 488, 538, 541, 596, 1000, 1022
MfeI CAATTG 1 cut(s) 6
MflI RGATCY 1 cut(s) 469
MhlI GDGCHC 1 cut(s) 364
MlsI TGGCCA 1 cut(s) 231
MluCI AATT 5 cut(s) 6, 338, 623, 927, 1045
MluNI TGGCCA 1 cut(s) 231
MlyI GAGTC 4 cut(s) 263, 689, 751, 1034
Mox20I TGGCCA 1 cut(s) 231
Mph1103I ATGCAT 1 cut(s) 853
MroXI GAANNNNTTC 2 cut(s) 30, 992
MscI TGGCCA 1 cut(s) 231
MseI TTAA 4 cut(s) 18, 630, 765, 960
MslI CAYNNNNRTG 3 cut(s) 237, 779, 782
Msp20I TGGCCA 1 cut(s) 231
MspA1I CMGCKG 2 cut(s) 689, 857
MspCI CTTAAG 1 cut(s) 629
MspR9I CCNGG 1 cut(s) 482
MunI CAATTG 1 cut(s) 6
Mva1269I GAATGC 1 cut(s) 971
MvaI CCWGG 1 cut(s) 482
NdeII GATC 8 cut(s) 148, 282, 298, 394, 418, 469, 531, 871
NlaIII CATG 6 cut(s) 110, 452, 722, 748, 808, 827
NlaIV GGNNCC 4 cut(s) 361, 552, 553, 786
NmuCI GTSAC 2 cut(s) 653, 859
NsiI ATGCAT 1 cut(s) 853
OliI CACNNNNGTG 3 cut(s) 237, 779, 782
PctI GAATGC 1 cut(s) 971
PdmI GAANNNNTTC 2 cut(s) 30, 992
PfeI GAWTC 4 cut(s) 36, 491, 600, 913
PflMI CCANNNNNTGG 1 cut(s) 189
PkrI GCNGC 2 cut(s) 592, 688
PleI GAGTC 4 cut(s) 263, 689, 751, 1034
PpsI GAGTC 4 cut(s) 263, 689, 751, 1034
Ppu21I YACGTR 1 cut(s) 1021
PpuMI RGGWCCY 1 cut(s) 551
PshBI ATTAAT 1 cut(s) 960
Psp5II RGGWCCY 1 cut(s) 551
Psp6I CCWGG 1 cut(s) 480
PspFI CCCAGC 1 cut(s) 881
PspGI CCWGG 1 cut(s) 480
PspN4I GGNNCC 4 cut(s) 361, 552, 553, 786
PspPI GGNCC 2 cut(s) 551, 953
PspPPI RGGWCCY 1 cut(s) 551
PstNI CAGNNNCTG 2 cut(s) 603, 617
PsuI RGATCY 1 cut(s) 469
PvuII CAGCTG 2 cut(s) 689, 857
RsaI GTAC 1 cut(s) 134
RsaNI GTAC 1 cut(s) 133
RseI CAYNNNNRTG 3 cut(s) 237, 779, 782
SaqAI TTAA 4 cut(s) 18, 630, 765, 960
SatI GCNGC 2 cut(s) 591, 687
Sau3AI GATC 8 cut(s) 148, 282, 298, 394, 418, 469, 531, 871
Sau96I GGNCC 2 cut(s) 551, 953
SchI GAGTC 4 cut(s) 263, 689, 751, 1034
ScrFI CCNGG 1 cut(s) 482
SduI GDGCHC 1 cut(s) 364
SexAI ACCWGGT 1 cut(s) 480
SfaNI GCATC 5 cut(s) 577, 598, 838, 860, 1039
SinI GGWCC 2 cut(s) 551, 953
SmiMI CAYNNNNRTG 3 cut(s) 237, 779, 782
SmlI CTYRAG 1 cut(s) 629
SmoI CTYRAG 1 cut(s) 629
Sse9I AATT 5 cut(s) 6, 338, 623, 927, 1045
SspMI CTAG 3 cut(s) 71, 276, 999
StyD4I CCNGG 1 cut(s) 480
TaaI ACNGT 4 cut(s) 187, 227, 236, 781
TaiI ACGT 3 cut(s) 115, 125, 1023
TaqI TCGA 1 cut(s) 34
TasI AATT 5 cut(s) 6, 338, 623, 927, 1045
TfiI GAWTC 4 cut(s) 36, 491, 600, 913
Tru1I TTAA 4 cut(s) 18, 630, 765, 960
Tru9I TTAA 4 cut(s) 18, 630, 765, 960
TscAI CASTG 6 cut(s) 241, 292, 411, 421, 784, 874
TseFI GTSAC 2 cut(s) 653, 859
TseI GCWGC 2 cut(s) 590, 686
Tsp45I GTSAC 2 cut(s) 653, 859
TspDTI ATGAA 4 cut(s) 62, 113, 123, 437
TspRI CASTG 6 cut(s) 241, 292, 411, 421, 784, 874
Van91I CCANNNNNTGG 1 cut(s) 189
Vha464I CTTAAG 1 cut(s) 629
VpaK11BI GGWCC 2 cut(s) 551, 953
VspI ATTAAT 1 cut(s) 960
XbaI TCTAGA 1 cut(s) 275
XcmI CCANNNNNNNNNTGG 1 cut(s) 830
XmnI GAANNNNTTC 2 cut(s) 30, 992
XspI CTAG 3 cut(s) 71, 276, 999
Zsp2I ATGCAT 1 cut(s) 853
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.